FastQCFastQC Report
Thu 26 May 2016
SRR1364287_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364287_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1123630
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT89340.7951015903811753No Hit
TATCAACGCAGAGTACTTTTTTTTT65530.5831990957877593No Hit
GGTATCAACGCAGAGTACTTTTTTT64580.5747443553482908No Hit
ACGCAGAGTACTTTTTTTTTTTTTT38730.3446864181269635No Hit
GTACTTTTTTTTTTTTTTTTTTTTT32570.289864101172094No Hit
GAGTACTTTTTTTTTTTTTTTTTTT29550.26298692630136256No Hit
GTGGTATCAACGCAGAGTACATGGG20230.18004147272678728No Hit
GGTATCAACGCAGAGTACATGGGAT12890.11471747817342007No Hit
TGCATGCACTGCCTCAGTGACCAGT12220.10875466123190017No Hit
GCAGAGTACTTTTTTTTTTTTTTTT11780.1048387814494095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGA901.3460522E-1015.8372316
CGCGGGT508.7352695E-515.19494315
TATCACG456.756779E-414.7787832
ACGGTGA602.56321E-514.25223710
TAACGCA1006.5847416E-1014.2516024
CGGTGAT602.5664414E-514.25033411
TGCCGCG602.5750764E-514.24525912
CGCGCCA759.64872E-713.93552110
ACCTACA551.95826E-413.8191212
AGACGAA500.001497322813.30208910
GCGAGAC500.001502859613.29557613
ACGAAAT500.001502859613.29557612
GGTATCA19650.013.1044221
TATAACG1102.739398E-912.9554262
ACCGAGT1051.9843355E-812.6697858
AGTCTAC905.387301E-712.669221
TAGACAG1301.9645086E-1012.4289035
GGTACAT3100.012.2605351
GTATTAC550.003062905712.0933471
ACGTCTT550.00307518812.08688716
Produced by FastQC (version 0.11.5)