##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364287_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1123630
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.591584418358355	31.0	30.0	34.0	26.0	34.0
2	29.601987308989614	31.0	30.0	34.0	26.0	34.0
3	29.639013732278418	31.0	30.0	34.0	26.0	34.0
4	33.007623505958364	35.0	33.0	37.0	27.0	37.0
5	32.580469549584826	35.0	33.0	37.0	25.0	37.0
6	32.47112305652217	35.0	33.0	37.0	25.0	37.0
7	32.201092886448386	35.0	32.0	37.0	22.0	37.0
8	32.09137349483371	35.0	32.0	37.0	21.0	37.0
9	32.87031407135801	37.0	32.0	39.0	17.0	39.0
10	32.944948960066924	37.0	32.0	39.0	17.0	39.0
11	32.904062725274336	37.0	32.0	39.0	17.0	39.0
12	32.82310191077134	37.0	32.0	39.0	17.0	39.0
13	32.878607726742786	37.0	32.0	39.0	17.0	39.0
14	33.77515908261616	38.0	32.0	41.0	17.0	41.0
15	33.8092726253304	38.0	33.0	41.0	17.0	41.0
16	33.74383471427427	38.0	33.0	41.0	17.0	41.0
17	33.70899851374563	38.0	33.0	41.0	17.0	41.0
18	33.66024225056291	38.0	33.0	41.0	16.0	41.0
19	33.574639338572304	38.0	32.0	41.0	11.0	41.0
20	33.481210896825466	38.0	32.0	41.0	10.0	41.0
21	33.41883805167181	38.0	32.0	41.0	10.0	41.0
22	33.36087858102756	38.0	32.0	41.0	8.0	41.0
23	33.09231063606347	38.0	32.0	41.0	2.0	41.0
24	32.99723663483531	38.0	32.0	41.0	2.0	41.0
25	32.830219912248694	38.0	32.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.5823863032124059
1101	2	0.7346171905832435
1101	3	-1.302629786209657
1101	4	-0.17061115712838415
1101	5	0.7026143988566389
1101	6	1.0593386935386278
1101	7	1.3370105253707791
1101	8	1.341914385716251
1101	9	2.166913013194126
1101	10	1.849241469014018
1101	11	1.7487303039619917
1101	12	1.8515093277091665
1101	13	1.6484027904472782
1101	14	1.8308833362431827
1101	15	1.8360519377317388
1101	16	1.7245753221863112
1101	17	1.8050420992962728
1101	18	1.799182551770869
1101	19	1.6176937453549058
1101	20	2.1545770764685415
1101	21	1.8950216659219166
1101	22	2.062085480104443
1101	23	2.4427988547783883
1101	24	2.228403663376781
1101	25	2.4616713780928166
1102	1	0.5320764026019482
1102	2	0.5990265421857011
1102	3	0.6422715814037296
1102	4	0.6068833711326249
1102	5	0.8256456779847738
1102	6	0.8733437744727155
1102	7	0.9485761559838011
1102	8	0.9064994035760208
1102	9	1.6643074903824342
1102	10	1.0889912660870422
1102	11	1.2049308400215395
1102	12	1.29937112479724
1102	13	1.3097142959757662
1102	14	1.3328583667410143
1102	15	1.214546098858733
1102	16	1.1889531334969874
1102	17	1.3600480859947979
1102	18	1.2597774091984064
1102	19	1.2244950988911683
1102	20	1.3575087559953474
1102	21	1.560057496759356
1102	22	1.5856565183412457
1102	23	1.835331894488121
1102	24	1.9080430255355125
1102	25	1.7572970280626734
1103	1	-2.1798600387440814
1103	2	-2.106575969450496
1103	3	-2.2313720048069463
1103	4	-2.029590878426948
1103	5	-4.996592996002931
1103	6	-8.20161800944706
1103	7	-9.80545624028439
1103	8	-9.74317738778132
1103	9	-10.701233601329303
1103	10	-8.156544478800914
1103	11	-11.41094442817651
1103	12	-10.229755355004787
1103	13	-10.54592488938765
1103	14	-10.836351468672781
1103	15	-11.963904144833041
1103	16	-5.375054516573961
1103	17	-3.643999733803529
1103	18	-2.7781679023911785
1103	19	-2.3885662319195617
1103	20	-7.588683688531358
1103	21	-4.388068030211258
1103	22	-10.622249240462164
1103	23	-11.52793679711668
1103	24	-13.73724662836047
1103	25	-15.300811978593437
1104	1	0.46649883154172755
1104	2	0.5533800596379521
1104	3	0.45034624312134497
1104	4	0.5100911780587651
1104	5	0.5323364814196978
1104	6	0.6265944038795084
1104	7	0.4317905041769663
1104	8	0.8422276519616219
1104	9	0.954558720673063
1104	10	0.8377460674531036
1104	11	0.825832165769036
1104	12	0.8385111763667652
1104	13	0.6943423184594693
1104	14	0.741734105485854
1104	15	0.6622450180284503
1104	16	0.609298603669572
1104	17	0.4951343774767025
1104	18	0.7450216798851628
1104	19	0.7329423085844127
1104	20	1.173132648353473
1104	21	1.0039055872418103
1104	22	0.6920649824365341
1104	23	1.0800117907405848
1104	24	1.245310449265105
1104	25	1.420662738132421
1105	1	0.3975810386969947
1105	2	0.4888224592812662
1105	3	0.0626796225780133
1105	4	0.3626650138289449
1105	5	0.6083452756020051
1105	6	0.7938926527302712
1105	7	0.8067772052823869
1105	8	0.5261828815789542
1105	9	0.7173971506347812
1105	10	0.3389661833764137
1105	11	0.9622317783330274
1105	12	1.173162714579945
1105	13	0.9967238199277304
1105	14	0.8084296761083607
1105	15	0.5995077519121921
1105	16	0.9072125990264865
1105	17	0.9497151932447352
1105	18	0.9126289550723428
1105	19	0.7764169330636008
1105	20	0.911877478696475
1105	21	0.8072181650749357
1105	22	0.812094193881272
1105	23	1.3003460311661499
1105	24	1.208881317767478
1105	25	1.4343170898689692
1106	1	0.048632123830476104
1106	2	0.24728032129718258
1106	3	0.10834224127433956
1106	4	-1.183599756218598
1106	5	-0.009535151641713924
1106	6	-0.14082594397334702
1106	7	0.4088245198765179
1106	8	0.2575578043396405
1106	9	0.7844648311856091
1106	10	0.3851048484289379
1106	11	0.26574443296927797
1106	12	0.6469473776904522
1106	13	0.3457660524905606
1106	14	-1.7446770177542703E-4
1106	15	0.44127687888730804
1106	16	0.1128356207543817
1106	17	0.20367678166324765
1106	18	0.3423619907978903
1106	19	-0.9935114392992261
1106	20	0.18162425986648145
1106	21	-0.30743964902107734
1106	22	0.4338750486209264
1106	23	0.24419526003410397
1106	24	0.5603189147106917
1106	25	0.7959559503842328
1107	1	0.32013651081460637
1107	2	0.08457847920348627
1107	3	-0.02255129663837252
1107	4	0.15010465463173261
1107	5	0.2776549803132369
1107	6	0.2183179503621986
1107	7	0.1406202629003701
1107	8	-0.0936973692869536
1107	9	0.893287787956389
1107	10	0.4941121401806541
1107	11	0.4997477993593904
1107	12	0.36506967311630945
1107	13	0.19094790664296823
1107	14	0.47729709697876643
1107	15	0.45013846121627665
1107	16	0.27919056137150733
1107	17	-0.09370610124792478
1107	18	0.056711228649682255
1107	19	0.3986381515210766
1107	20	0.594089638177131
1107	21	0.36972134104932763
1107	22	0.44362313461461156
1107	23	0.5763769446108853
1107	24	0.791633565416312
1107	25	0.5427519132283294
1108	1	-0.1342098971399679
1108	2	0.09885310476105857
1108	3	0.1829712545622968
1108	4	-0.1198188198055945
1108	5	0.04085648019933075
1108	6	-0.054885902432843636
1108	7	-0.40898534474148107
1108	8	-0.05173000127082972
1108	9	0.4993218167465301
1108	10	0.20205762738842026
1108	11	0.24966625674746723
1108	12	0.04991276894692476
1108	13	-0.4431794193444816
1108	14	-0.2705193039170837
1108	15	-0.36900189291520036
1108	16	-0.9018424403552032
1108	17	-0.9858980538013284
1108	18	-0.8816213930013106
1108	19	-0.6504719758223345
1108	20	-0.19505174924240265
1108	21	-0.42182617588477456
1108	22	-0.3211961457936283
1108	23	-0.7572048550781325
1108	24	-0.42692740044658706
1108	25	-0.618589481411771
1109	1	0.23648547800303987
1109	2	0.2948411468260588
1109	3	0.18965215918910516
1109	4	0.1971274426688865
1109	5	0.14624096610813808
1109	6	0.4732903783127327
1109	7	0.5169782789372803
1109	8	0.4935202444986473
1109	9	1.2297901570036913
1109	10	0.3971315045939434
1109	11	0.3874477939104892
1109	12	0.5130272231191526
1109	13	0.45866586419909083
1109	14	0.15779346121512106
1109	15	-0.11335041476123564
1109	16	0.252120851565266
1109	17	0.16650829156393598
1109	18	0.1309500509034791
1109	19	0.3511130238239488
1109	20	0.7359278890922667
1109	21	0.8247428091056079
1109	22	0.45944453801876506
1109	23	0.784937596078997
1109	24	0.3664408488209858
1109	25	0.5647783287639427
1110	1	0.16889863970095575
1110	2	0.21241935702112613
1110	3	0.21835379685175127
1110	4	0.4267129819615221
1110	5	0.44752727306263296
1110	6	0.4199207430180252
1110	7	0.5421070414510325
1110	8	0.33007274053809965
1110	9	-3.310912937850361
1110	10	-1.1232422122747252
1110	11	0.044393659729045964
1110	12	0.34945149590155467
1110	13	-0.009725305412715102
1110	14	0.4754174483113047
1110	15	0.4895361663753235
1110	16	-0.1744102180084397
1110	17	0.1842953738497144
1110	18	0.2729842709345931
1110	19	0.1642000646468773
1110	20	0.7535442067599973
1110	21	0.3444670212515817
1110	22	0.6705689241648969
1110	23	0.844007174571999
1110	24	0.9097263840392387
1110	25	0.8957531131211809
1111	1	-0.003204227644435065
1111	2	-0.02966326574004796
1111	3	-0.07404719410511262
1111	4	-0.16103426428504264
1111	5	0.10248259515348934
1111	6	9.259690845411228E-4
1111	7	0.042559078876024614
1111	8	0.21555460107992985
1111	9	0.7800287460353559
1111	10	0.3853303652767366
1111	11	0.6150516206667973
1111	12	0.6309731142254122
1111	13	0.27212973906203075
1111	14	0.23236561906851705
1111	15	0.2212709264698276
1111	16	0.14439447911752268
1111	17	0.32067921785886
1111	18	0.07611465251034844
1111	19	0.2450550099303328
1111	20	0.43418379938255214
1111	21	0.39008396121217004
1111	22	0.3296457639542325
1111	23	0.6120528192974746
1111	24	0.6172955161871343
1111	25	0.8258677686943585
1112	1	-0.5438956590638568
1112	2	-0.5900408551011722
1112	3	-0.5928980116881455
1112	4	-0.6890120482306301
1112	5	-0.604850433768565
1112	6	-0.732785789248716
1112	7	-0.45522349387677963
1112	8	-0.98547651751214
1112	9	-0.5848603975244053
1112	10	-0.7206205767567937
1112	11	-0.46949532880390876
1112	12	-1.1033841750816968
1112	13	-1.0290052374953191
1112	14	-2.068855010465942
1112	15	-1.2405890264359556
1112	16	-1.2142166295065664
1112	17	-1.2427651659022914
1112	18	-1.4131608188048617
1112	19	-0.8900639044554879
1112	20	-0.9441785276327508
1112	21	-1.2227371255360922
1112	22	-0.7690413878007618
1112	23	-0.5178173880901653
1112	24	-0.7951701256282284
1112	25	-0.6543234135256348
1113	1	-2.303956158673536
1113	2	-2.444031877739736
1113	3	-2.3681224457240546
1113	4	-2.420618215287618
1113	5	-2.288788763198699
1113	6	-2.2476277096234263
1113	7	-2.7278327834942644
1113	8	-3.2349983754356373
1113	9	-11.937124253886623
1113	10	-5.4476599990831005
1113	11	-4.008673705072454
1113	12	-14.234473168055935
1113	13	-5.7026488752783
1113	14	-4.717810904298883
1113	15	-4.167872398801293
1113	16	-4.136562453081897
1113	17	-4.027884213521343
1113	18	-4.133701412091355
1113	19	-5.180188806875471
1113	20	-16.493114905462573
1113	21	-18.429470146613376
1113	22	-13.303223667269926
1113	23	-18.771896232742783
1113	24	-20.25272281883666
1113	25	-17.00284019728832
1114	1	-0.017640692387185908
1114	2	0.10469742263265758
1114	3	0.12122962621953093
1114	4	-0.40594921124673533
1114	5	0.08432152917116298
1114	6	0.1438042292086834
1114	7	0.10446754928443625
1114	8	0.21675564564034744
1114	9	0.9758580771489562
1114	10	0.16462805257683044
1114	11	0.18954652311865772
1114	12	0.1698153193856271
1114	13	0.13338531377169716
1114	14	0.17493630273700944
1114	15	0.1326941173226288
1114	16	0.02668332775955662
1114	17	-0.15457880982908279
1114	18	-0.1337994629589545
1114	19	-0.21889017760678797
1114	20	-0.3934843294384862
1114	21	-0.5971331685609229
1114	22	-0.6862885987333556
1114	23	-0.4337316657512602
1114	24	-0.05144518631942674
1114	25	-0.36714175817890293
1115	1	-0.4722437044610821
1115	2	-0.35098242719028505
1115	3	-0.49448691458852423
1115	4	-0.19861272078212266
1115	5	-0.6785872979972325
1115	6	-0.69937679387251
1115	7	-0.8127686809301622
1115	8	-0.5785240164612802
1115	9	-0.13232571908808666
1115	10	-0.611112380035479
1115	11	-0.40454366844241463
1115	12	-0.4567259153086809
1115	13	-0.7247093148387407
1115	14	-0.8769592559472343
1115	15	-0.9960408969697951
1115	16	-0.7767639182833648
1115	17	-0.5807138838510113
1115	18	-0.8641318150217572
1115	19	-0.6698957665824281
1115	20	-1.119680839528506
1115	21	-0.686301648444875
1115	22	-1.176456268002525
1115	23	-0.8316215074680571
1115	24	-0.4680158591297001
1115	25	-0.2806790251271494
1116	1	-1.2779104701278463
1116	2	-0.9376451338529925
1116	3	-1.0036919437935552
1116	4	-0.944958627128031
1116	5	-2.3126948121047484
1116	6	-1.879915354411068
1116	7	-3.092552860714342
1116	8	-2.147820125757388
1116	9	-1.2527613195236889
1116	10	-2.2147932037163045
1116	11	-1.8261930100917567
1116	12	-2.9353418939246616
1116	13	-2.716500226629652
1116	14	-2.0956583946463745
1116	15	-3.5981812791101753
1116	16	-3.870764512283955
1116	17	-4.904283827420954
1116	18	-3.68619497708492
1116	19	-2.9673831440698066
1116	20	-3.434315034162701
1116	21	-2.5398626820059107
1116	22	-2.3373452888915445
1116	23	-2.4693544052009564
1116	24	-2.947027838141679
1116	25	-2.5594039038520293
1201	1	0.8661969025509535
1201	2	0.8922290552048686
1201	3	0.6229489828473724
1201	4	1.076794321155532
1201	5	1.337085872369812
1201	6	1.442131765595235
1201	7	1.5566788783133134
1201	8	1.4377785924943893
1201	9	2.177307232993847
1201	10	1.8370703216166078
1201	11	1.8904881025077245
1201	12	2.03892333340179
1201	13	1.7882038944010006
1201	14	2.020636617567007
1201	15	2.0261509248138623
1201	16	1.951994398230056
1201	17	1.9341055823970237
1201	18	1.9977490252264332
1201	19	2.0601902018708884
1201	20	2.2774754735461755
1201	21	2.471914693036773
1201	22	2.253080997453111
1201	23	2.596398227898618
1201	24	2.53904102955984
1201	25	2.657363884344335
1202	1	0.9087444833810387
1202	2	1.0337971752297648
1202	3	1.0757579716114165
1202	4	1.1107822662533167
1202	5	1.1163075449874995
1202	6	1.3805223706333933
1202	7	1.275116654707979
1202	8	1.2665465683845838
1202	9	2.0945771907361745
1202	10	1.6412846237098364
1202	11	1.883721961059166
1202	12	1.9014417597129238
1202	13	1.6440806592321309
1202	14	1.664994071624072
1202	15	1.678355530572702
1202	16	1.6633974185071878
1202	17	1.6362610513261728
1202	18	1.674946260460814
1202	19	1.6379492348580769
1202	20	1.8681411137990622
1202	21	1.9453693397429674
1202	22	1.8524143744636206
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2211	9	0.8531903219704446
2211	10	0.13124642987009594
2211	11	0.5887751381740927
2211	12	0.6170519903772913
2211	13	0.48435864552799046
2211	14	0.3816708472472712
2211	15	0.3854337018324401
2211	16	0.36744800732888194
2211	17	0.12673421054523715
2211	18	0.26850615452695337
2211	19	-0.0570804614528555
2211	20	0.42355971467944187
2211	21	0.8622932343135545
2211	22	0.6358453943472426
2211	23	1.0441742414116035
2211	24	1.0093238330683576
2211	25	1.0808087299469236
2212	1	-0.13379667978763976
2212	2	-0.14035188372955432
2212	3	-0.08386443434340407
2212	4	-4.737109450019261E-4
2212	5	-0.08761326155149618
2212	6	0.1274059830989458
2212	7	-0.014284565464912191
2212	8	-0.1412090672595383
2212	9	0.2598658629148147
2212	10	-0.0818880613771995
2212	11	0.05714315626327959
2212	12	-0.04183315607630078
2212	13	-0.3075717563804261
2212	14	-0.23828484859358667
2212	15	0.08116923042901192
2212	16	-0.13763313292616175
2212	17	-0.26530185353332314
2212	18	-0.21434307127074703
2212	19	-0.05127296380868529
2212	20	0.1648103715664746
2212	21	0.43600604386281816
2212	22	0.042437458815733464
2212	23	0.2729600116417643
2212	24	0.25960871755147963
2212	25	0.26545869459547333
2213	1	-0.35411256459968854
2213	2	-0.366724170669805
2213	3	-0.38898008754489055
2213	4	-0.37866976207505587
2213	5	-0.20187888371761886
2213	6	-0.03734617895634784
2213	7	-0.037901295047195305
2213	8	-0.2381754307822277
2213	9	0.5782092841497644
2213	10	-0.08285517064985015
2213	11	0.11350810962010627
2213	12	0.1893907300019464
2213	13	-0.3392027633569086
2213	14	-0.239954337169074
2213	15	-0.32944835642776127
2213	16	-0.5150628752819415
2213	17	-0.5744864626961288
2213	18	-0.6846465644671866
2213	19	-0.4545535877152673
2213	20	-0.02358527469570504
2213	21	-0.19837511322118928
2213	22	0.15550531868233008
2213	23	-0.16286690903049106
2213	24	0.12152105926267609
2213	25	0.1969026445018045
2214	1	-1.2008299719947182
2214	2	-0.8725397003265059
2214	3	-0.8540313467645362
2214	4	-0.7804030941514455
2214	5	-0.8682924466497504
2214	6	-0.8929464990211606
2214	7	-0.8590329429838981
2214	8	-0.913978970863603
2214	9	-10.976294148319674
2214	10	-3.983882183331602
2214	11	-1.9404795454309252
2214	12	-1.2495734186825018
2214	13	-1.0921178127735516
2214	14	-1.1043235875220958
2214	15	-1.199682985875043
2214	16	-1.2113118265154839
2214	17	-1.0370235391080342
2214	18	-1.37114445150808
2214	19	-1.6633018926201366
2214	20	-1.1367077891870352
2214	21	-1.716705279901138
2214	22	-1.5470823670496756
2214	23	-1.9067542110218163
2214	24	-2.1006171759415437
2214	25	-9.990988598594615
2215	1	-0.5282747809783075
2215	2	-0.19950005992498987
2215	3	-0.27348968306557353
2215	4	-0.3556975579347963
2215	5	-0.2129529398925527
2215	6	-0.25398420643356445
2215	7	-0.4149936088193087
2215	8	-0.34916598425418144
2215	9	0.411842070913508
2215	10	0.3072105896272248
2215	11	0.32748978519646244
2215	12	0.3555756892484325
2215	13	0.23664390514647948
2215	14	0.050063399181695445
2215	15	-0.3839468557180368
2215	16	-0.19818120459606803
2215	17	-0.17248063603607733
2215	18	-0.08792999159551584
2215	19	-0.11254364819017582
2215	20	0.26290645446636063
2215	21	0.2647699406029673
2215	22	0.10293901544024209
2215	23	0.530043927153848
2215	24	0.7799005357623869
2215	25	0.7990212256301987
2216	1	-1.4863087988700165
2216	2	-1.5862392047966338
2216	3	-1.412008037209933
2216	4	-1.429884486265923
2216	5	-2.354292242527393
2216	6	-2.8738915246050034
2216	7	-2.1912640155859577
2216	8	-3.2241459399055543
2216	9	-1.719256877709789
2216	10	-2.313557461219819
2216	11	-2.0531425957805993
2216	12	-3.126706078412056
2216	13	-2.965476341508346
2216	14	-3.3843121322972465
2216	15	-2.8552444823051815
2216	16	-3.7032939307732597
2216	17	-4.149164401814424
2216	18	-3.6426095168805475
2216	19	-4.955526506425205
2216	20	-3.798287848565316
2216	21	-4.100908951361635
2216	22	-3.8671358653869916
2216	23	-3.4738917300821193
2216	24	-3.7127003055333425
2216	25	-3.5583661899203314
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	39673.0
3	4.0
4	572.0
5	5950.0
6	7788.0
7	6111.0
8	4901.0
9	3894.0
10	3541.0
11	3171.0
12	2928.0
13	2659.0
14	2608.0
15	2701.0
16	2814.0
17	2989.0
18	3449.0
19	4226.0
20	5434.0
21	7254.0
22	8913.0
23	10791.0
24	12140.0
25	13974.0
26	15705.0
27	17059.0
28	19331.0
29	22491.0
30	27712.0
31	33989.0
32	41540.0
33	51733.0
34	64337.0
35	79824.0
36	103872.0
37	149441.0
38	338111.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.055038684469864	17.945125115839758	11.52237254065853	24.47746365903185
2	17.802386752093014	21.394618638524268	35.90816816020377	24.894826449178947
3	20.192786053047758	25.61868730693138	28.715616987457604	25.47290965256326
4	12.595296877697818	15.925592340293736	35.178009138532246	36.3011016434762
5	12.988368014443392	39.13754515784994	32.7974631481676	15.076623679539065
6	32.27134930566805	35.59294577962593	17.723132352967355	14.412572561738667
7	29.563977397750456	32.510672691084785	19.80821200824619	18.117137902918568
8	28.76410395645188	33.45033404400212	19.159393068086224	18.62616893145978
9	28.713273228572877	14.426032774217212	18.389848841898722	38.47084515531119
10	16.038169243808806	30.743014650892803	30.50361108539113	22.715205019907266
11	36.14218304843305	22.292111823361825	21.203614672364672	20.362090455840455
12	24.059116457284755	26.78842413966508	28.21355619935116	20.938903203699006
13	29.50177419684997	20.87108748877499	25.654877550602656	23.972260763772386
14	22.589057250294818	20.57557350421645	27.584540417862623	29.2508288276261
15	24.953896707263674	27.863121281920744	23.599723736253566	23.58325827456202
16	23.65690259634495	28.113526787184444	24.888099321698096	23.34147129477251
17	23.05537012807847	27.498865183840216	26.252525522237946	23.19323916584337
18	22.18759735462451	25.92183146857504	29.04252004948953	22.84805112731092
19	23.59777791426224	25.570008073352685	28.709892482880843	22.122321529504234
20	23.83336997572701	25.09713201319838	28.75250006453215	22.316997946542454
21	24.619406359367268	24.60365213242807	28.228904467079897	22.548037041124765
22	24.12705391878938	24.85556592966944	28.042861605859425	22.97451854568175
23	22.939596383579786	25.092252893082765	28.742494717598454	23.225656005738994
24	23.246855772730953	25.06712995308691	28.535814953625373	23.150199320556762
25	23.025964712223328	24.737969772424577	28.955665331830716	23.280400183521383
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	485.0
1	485.0
2	718.5
3	952.0
4	952.0
5	952.0
6	2624.5
7	4297.0
8	4297.0
9	4297.0
10	4843.5
11	5390.0
12	5390.0
13	5390.0
14	5116.0
15	4842.0
16	4842.0
17	4842.0
18	7892.5
19	10943.0
20	10943.0
21	10943.0
22	17287.0
23	23631.0
24	23631.0
25	23631.0
26	34033.5
27	44436.0
28	44436.0
29	44436.0
30	53218.5
31	62001.0
32	62001.0
33	62001.0
34	71534.0
35	81067.0
36	81067.0
37	81067.0
38	90313.0
39	99559.0
40	99559.0
41	99559.0
42	110649.5
43	121740.0
44	121740.0
45	121740.0
46	133096.0
47	144452.0
48	144452.0
49	144452.0
50	146452.0
51	148452.0
52	148452.0
53	148452.0
54	138590.0
55	128728.0
56	128728.0
57	128728.0
58	114418.5
59	100109.0
60	100109.0
61	100109.0
62	84258.5
63	68408.0
64	68408.0
65	68408.0
66	52627.5
67	36847.0
68	36847.0
69	36847.0
70	28002.5
71	19158.0
72	19158.0
73	19158.0
74	13939.0
75	8720.0
76	8720.0
77	8720.0
78	7106.0
79	5492.0
80	5492.0
81	5492.0
82	3944.0
83	2396.0
84	2396.0
85	2396.0
86	1709.5
87	1023.0
88	1023.0
89	1023.0
90	744.0
91	465.0
92	465.0
93	465.0
94	249.0
95	33.0
96	33.0
97	33.0
98	18.5
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02767815028078638
2	9.789699456226693E-4
3	8.899726778387903E-5
4	0.003203901640219645
5	0.030971049188789904
6	0.00925571584952342
7	0.017977448092343567
8	0.024474248640566736
9	0.03283999181225136
10	0.03755684700479695
11	0.03826882514706798
12	0.0075647677616297185
13	0.0020469371590292177
14	0.004894849728113346
15	0.005873819673736016
16	0.007920756832765235
17	0.008899726778387903
18	0.014239562845420644
19	0.016108505468882105
20	0.014150565577636767
21	0.010857666669633241
22	0.009522707652875056
23	0.007831759564981354
24	0.005606827870384379
25	0.10190187161254151
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1123630.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.6410776412218
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.41418358228958	34.70757714069701
2	12.959776160682528	12.089158522464984
3	4.881281080963781	6.830046296575764
4	2.3181176028765575	4.32478012388994
5	1.3505438305839095	3.1495409830068613
6	0.8459016546743987	2.3672258851503973
7	0.5758688280841927	1.8801399905283969
8	0.4105602645158868	1.531917853894883
9	0.3102078488716309	1.3021585527724322
>10	1.6836891743363396	14.476721701355702
>50	0.15104776000886522	4.88522278226898
>100	0.09306557894542998	7.814564162342205
>500	0.003070204355681924	0.8768170477581506
>1k	0.002110765494531323	1.800083829902999
>5k	5.756633166903607E-4	1.9640451273913049
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	8934	0.7951015903811753	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6553	0.5831990957877593	No Hit
GGTATCAACGCAGAGTACTTTTTTT	6458	0.5747443553482908	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3873	0.3446864181269635	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3257	0.289864101172094	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2955	0.26298692630136256	No Hit
GTGGTATCAACGCAGAGTACATGGG	2023	0.18004147272678728	No Hit
GGTATCAACGCAGAGTACATGGGAT	1289	0.11471747817342007	No Hit
TGCATGCACTGCCTCAGTGACCAGT	1222	0.10875466123190017	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	1178	0.1048387814494095	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.899726778387903E-5	0.0	0.0	0.0	0.0
2	8.899726778387903E-5	0.0	0.0	0.0	0.0
3	8.899726778387903E-5	0.0	0.0	0.0	0.0
4	8.899726778387903E-5	0.0	0.0	8.899726778387903E-5	0.0
5	8.899726778387903E-5	0.0	0.0	8.899726778387903E-5	0.0
6	8.899726778387903E-5	0.0	0.0	8.899726778387903E-5	0.0
7	1.7799453556775806E-4	0.0	0.0	8.899726778387903E-5	0.0
8	1.7799453556775806E-4	0.0	0.0	8.899726778387903E-5	0.0
9	1.7799453556775806E-4	0.0	0.0	8.899726778387903E-5	0.0
10	1.7799453556775806E-4	0.0	0.0	8.899726778387903E-5	0.0
11	1.7799453556775806E-4	0.0	0.0	8.899726778387903E-5	0.0
12	2.669918033516371E-4	0.0	0.0	8.899726778387903E-5	0.0
13	2.669918033516371E-4	0.0	0.0	8.899726778387903E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACCGA	90	1.3460522E-10	15.837231	6
CGCGGGT	50	8.7352695E-5	15.194943	15
TATCACG	45	6.756779E-4	14.778783	2
ACGGTGA	60	2.56321E-5	14.252237	10
TAACGCA	100	6.5847416E-10	14.251602	4
CGGTGAT	60	2.5664414E-5	14.250334	11
TGCCGCG	60	2.5750764E-5	14.245259	12
CGCGCCA	75	9.64872E-7	13.935521	10
ACCTACA	55	1.95826E-4	13.819121	2
AGACGAA	50	0.0014973228	13.302089	10
GCGAGAC	50	0.0015028596	13.295576	13
ACGAAAT	50	0.0015028596	13.295576	12
GGTATCA	1965	0.0	13.104422	1
TATAACG	110	2.739398E-9	12.955426	2
ACCGAGT	105	1.9843355E-8	12.669785	8
AGTCTAC	90	5.387301E-7	12.66922	1
TAGACAG	130	1.9645086E-10	12.428903	5
GGTACAT	310	0.0	12.260535	1
GTATTAC	55	0.0030629057	12.093347	1
ACGTCTT	55	0.003075188	12.086887	16
>>END_MODULE