FastQCFastQC Report
Thu 26 May 2016
SRR1364286_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364286_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2136143
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT145200.6797297746452368No Hit
TATCAACGCAGAGTACTTTTTTTTT86270.4038587304314365No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA78380.3669230009414164No Hit
GGTATCAACGCAGAGTACTTTTTTT60240.28200359245612305No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN56820.2659934283425782No Hit
GTACTTTTTTTTTTTTTTTTTTTTT56340.26374638776523857No Hit
ACGCAGAGTACTTTTTTTTTTTTTT35200.16478297567157255No Hit
GAGTACTTTTTTTTTTTTTTTTTTT33410.1564033868519102No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA28090.13149868711972934No Hit
GTGGTATCAACGCAGAGTACATGGG25350.11867183049074899No Hit
GAACAAAAAAAAAAAAAAAAAAAAA22370.10472145357309881No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGG1200.015.05339614
CGCATAT1850.014.3767428
AAGACGG400.005267485514.2550785
CGCGCCA954.9130904E-913.99840810
CGACGGT1500.013.94452815
CTCAACG1500.013.9252053
CGGTTTG2400.013.8649714
TAACGCA2400.013.8591034
CGCGACC551.9625803E-413.8166110
CTCGACG1450.013.74413813
CGAGGTT500.001495843513.304744
TTAGGCA3150.013.2745694
CATTCCG2800.013.2306389
AAATCCG1550.012.88130819
GGTCGGT2600.012.77469911
TATAACG2700.012.6592762
CGCCAGT1001.4633224E-712.33699912
GGTATCA30700.012.2469221
ATAACGC2800.012.2071593
GTCTCGG1950.012.18927218
Produced by FastQC (version 0.11.5)