FastQCFastQC Report
Thu 26 May 2016
SRR1364286_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364286_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2136143
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT333851.562863534885071No Hit
TATCAACGCAGAGTACTTTTTTTTT249251.166822633128962No Hit
GGTATCAACGCAGAGTACTTTTTTT228131.0679528477260183No Hit
GTACTTTTTTTTTTTTTTTTTTTTT160250.7501838594139063No Hit
ACGCAGAGTACTTTTTTTTTTTTTT116240.5441583264790794No Hit
GAGTACTTTTTTTTTTTTTTTTTTT104980.4914464996023206No Hit
GTGGTATCAACGCAGAGTACTTTTT43200.2022336519605663No Hit
ATCAACGCAGAGTACTTTTTTTTTT42440.19867583771311192No Hit
GTGGTATCAACGCAGAGTACATGGG39390.18439776737793304No Hit
GCAGAGTACTTTTTTTTTTTTTTTT36010.1685748566458332No Hit
GGTATCAACGCAGAGTACATGGGAT26040.12190195132067469No Hit
GTATAACGCAGAGTACTTTTTTTTT25950.12148063121242353No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAC250.006042212218.99487314
TATCACG2150.016.789312
TAACGCA3550.015.522394
TATAACG3550.015.5198452
CGTATCA508.722513E-515.1987452
TCGACCG456.780056E-414.77309818
ACAACGC851.7180355E-814.5292323
ATAACGC4000.014.4873223
ATCACGC2500.014.4398213
TCACGCA2600.014.251164
GTATCAC3050.013.7063541
GGTATCA55600.013.6704971
TTGCGCG2800.012.88877418
CGTCTTA1052.0025254E-812.66324915
GGCGTAG853.9396145E-612.29569310
TTTGCGC2950.012.23398517
ATTTGCG3050.012.14426316
ACGATTA550.003063457312.0938766
CATTCCG2200.012.0933099
CCGGCTA550.003070451612.09019411
Produced by FastQC (version 0.11.5)