##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364286_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2136143 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.643826747553884 31.0 30.0 34.0 26.0 34.0 2 29.651431575507818 31.0 30.0 34.0 26.0 34.0 3 29.686361821282564 31.0 30.0 34.0 26.0 34.0 4 33.02667096725266 35.0 33.0 37.0 27.0 37.0 5 32.641310062107266 35.0 33.0 37.0 26.0 37.0 6 32.500957566979366 35.0 33.0 37.0 25.0 37.0 7 32.222938258346936 35.0 32.0 37.0 22.0 37.0 8 32.09981166991161 35.0 32.0 37.0 19.0 37.0 9 32.76957488332944 37.0 32.0 39.0 16.0 39.0 10 32.944435367856926 37.0 32.0 39.0 17.0 39.0 11 32.87538521531564 37.0 32.0 39.0 17.0 39.0 12 32.777044888848735 37.0 32.0 39.0 17.0 39.0 13 32.853469547684774 37.0 32.0 39.0 17.0 39.0 14 33.76107404794529 38.0 32.0 41.0 17.0 41.0 15 33.78796363352079 38.0 33.0 41.0 17.0 41.0 16 33.76855716120129 38.0 33.0 41.0 17.0 41.0 17 33.78850807272734 38.0 33.0 41.0 17.0 41.0 18 33.78378366991348 38.0 33.0 41.0 17.0 41.0 19 33.7284765111699 39.0 33.0 41.0 15.0 41.0 20 33.57604898173952 39.0 32.0 41.0 10.0 41.0 21 33.52547277967814 39.0 32.0 41.0 10.0 41.0 22 33.47961114962809 39.0 33.0 41.0 8.0 41.0 23 33.18570151904624 38.0 32.0 41.0 2.0 41.0 24 33.099135685204594 38.0 32.0 41.0 2.0 41.0 25 32.958220025532 38.0 32.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.16803908990661398 1101 2 0.2768560226916641 1101 3 -1.4834213704591832 1101 4 -0.5469238959892238 1101 5 0.23716296516736435 1101 6 0.47011606448483434 1101 7 0.6379622985842559 1101 8 0.8060745098540245 1101 9 1.3822864876731416 1101 10 1.10941798107676 1101 11 0.9068958432837348 1101 12 1.0826757958451694 1101 13 0.8906789305107665 1101 14 1.028056782819995 1101 15 1.122575089579108 1101 16 1.0078106500875208 1101 17 0.9359896279048527 1101 18 0.9912917612700696 1101 19 1.0113286949999463 1101 20 1.1494218842218373 1101 21 1.0504482807594613 1101 22 1.1250622510629071 1101 23 1.3466111480591891 1101 24 1.313116849873154 1101 25 1.2925229440202983 1102 1 0.5834651435456024 1102 2 0.5448309569133158 1102 3 0.6166042800155758 1102 4 0.5797212160645202 1102 5 0.7273908623548877 1102 6 0.821041161738215 1102 7 0.9025265970811063 1102 8 1.0247393933086464 1102 9 1.6687250966034597 1102 10 1.1168617525851658 1102 11 1.037323653625542 1102 12 1.0764055242227002 1102 13 0.9801783654162506 1102 14 0.9628153416590806 1102 15 1.0380216785326724 1102 16 1.2011189004675131 1102 17 1.1213173883271779 1102 18 1.2126323962145307 1102 19 1.233930944998363 1102 20 1.219045188144861 1102 21 1.4614554147277445 1102 22 1.3731448127351271 1102 23 1.6823054825152397 1102 24 1.7371771632121806 1102 25 1.7177229673185508 1103 1 -2.0050581781445835 1103 2 -1.9341656087240189 1103 3 -1.9390228756145085 1103 4 -1.7767574515882778 1103 5 -4.4626212933850375 1103 6 -8.269693015662359 1103 7 -9.461108213247854 1103 8 -9.933042599109886 1103 9 -10.889707276719324 1103 10 -8.632617112781471 1103 11 -11.082455541508256 1103 12 -10.359048839272983 1103 13 -11.62998485834456 1103 14 -10.773857891059873 1103 15 -10.903269514902249 1103 16 -5.691917910920804 1103 17 -3.5584868749690806 1103 18 -3.121756631399503 1103 19 -2.839635690668729 1103 20 -7.070218706134373 1103 21 -4.112304663186155 1103 22 -9.49784995639125 1103 23 -10.384504709755365 1103 24 -11.573389177104598 1103 25 -12.200872019537144 1104 1 0.3882599917146159 1104 2 0.512387994682431 1104 3 0.56107593753649 1104 4 0.6892265758417722 1104 5 0.7214227469695231 1104 6 0.8599577192528756 1104 7 0.599028985478121 1104 8 0.8364390501361676 1104 9 1.2377338021505437 1104 10 1.0244351757432284 1104 11 1.0126099843413883 1104 12 1.0018622543521758 1104 13 0.9091997088599015 1104 14 0.9761872720060722 1104 15 0.9607035739726015 1104 16 0.6442213925859122 1104 17 0.6007308158585118 1104 18 0.898642525233285 1104 19 1.0213905839299855 1104 20 1.233836634079914 1104 21 1.0069415925228782 1104 22 0.824094201447906 1104 23 1.3528408416523234 1104 24 1.4798177643070858 1104 25 1.511761757101489 1105 1 0.2744514478473761 1105 2 0.38267364966536377 1105 3 0.028202490030842853 1105 4 0.3602127629948342 1105 5 0.46928504446770347 1105 6 0.39058597796775985 1105 7 0.5541937333020925 1105 8 0.4379406399207575 1105 9 0.5947238823141632 1105 10 0.09283749737475233 1105 11 0.7692223347239064 1105 12 0.8558367519871126 1105 13 0.6640438239087416 1105 14 0.6186014520240022 1105 15 0.31026381131390224 1105 16 0.6514684454366204 1105 17 0.6577509813349636 1105 18 0.6015806163852773 1105 19 0.6608803052033068 1105 20 0.9328594761367199 1105 21 0.8741548934132553 1105 22 0.621291711165064 1105 23 1.079695852502205 1105 24 1.0973520776550174 1105 25 1.195128122806544 1106 1 -0.2284797306736408 1106 2 -0.04926107219676368 1106 3 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1108 25 -0.18372376519865696 1109 1 0.4543042065706011 1109 2 0.5123300525076537 1109 3 0.4470300967448786 1109 4 0.436299385161 1109 5 0.46829044809952336 1109 6 0.7090926479155684 1109 7 0.8311939486270816 1109 8 0.49869304694650296 1109 9 1.4245197337194142 1109 10 0.6116835605162407 1109 11 0.8863898883244516 1109 12 0.8243787967542033 1109 13 0.8604355923420925 1109 14 0.5177575831466683 1109 15 0.3258389853682644 1109 16 0.7243559535113064 1109 17 0.7239121469608989 1109 18 0.5758390268767286 1109 19 0.8248502827479811 1109 20 1.1212996004099978 1109 21 1.1345648311740817 1109 22 0.813879404829386 1109 23 1.0050806177466995 1109 24 0.9458401886396715 1109 25 1.0109175964907564 1110 1 -0.010575491030270001 1110 2 0.07716815205854743 1110 3 -0.07084666959987018 1110 4 0.11385745558452953 1110 5 0.11330656144748019 1110 6 0.2098776123781505 1110 7 0.1878294403418863 1110 8 0.10840786008023073 1110 9 -3.610034513736746 1110 10 -1.3589520772547203 1110 11 -0.4643502459843134 1110 12 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2210 17 0.4832658554768443 2210 18 0.16368114506830267 2210 19 0.5192241595919 2210 20 0.6282486236682203 2210 21 0.6937187956726518 2210 22 0.7595186503270668 2210 23 0.8921185885026262 2210 24 0.831968322164343 2210 25 1.025410350113944 2211 1 0.08405455593996791 2211 2 0.20764121657521528 2211 3 0.19980858351011932 2211 4 0.18512189036047744 2211 5 -0.41404203360649916 2211 6 0.23639332626915888 2211 7 0.44255209624079583 2211 8 0.37134115302338344 2211 9 1.0340617502819214 2211 10 0.2687878254759468 2211 11 0.61769270964529 2211 12 0.7239861721331664 2211 13 0.6199171694783843 2211 14 0.6399329139209087 2211 15 0.5097765866095685 2211 16 0.6154366104520719 2211 17 0.521371079739616 2211 18 0.5432313878386736 2211 19 0.29008636714922176 2211 20 0.6474174349685455 2211 21 0.8863656733669458 2211 22 0.731612374599564 2211 23 1.175582976779637 2211 24 1.231258324173922 2211 25 1.380327665572807 2212 1 0.15633389515122076 2212 2 0.18889658787101027 2212 3 0.2263809806199646 2212 4 0.2563755110117185 2212 5 0.1066712744751186 2212 6 0.24341977691689465 2212 7 0.3167412295125871 2212 8 0.16278209264205756 2212 9 0.7268856990994408 2212 10 0.4294102591901776 2212 11 0.4496666409563588 2212 12 0.41244305853224716 2212 13 0.13987292591598788 2212 14 0.1326215856591375 2212 15 0.35438141838854165 2212 16 0.3803648340252934 2212 17 0.26610402687015267 2212 18 0.3095171566899282 2212 19 0.42430669372400587 2212 20 0.7017335695453681 2212 21 0.9604139593267789 2212 22 0.29892227051578857 2212 23 0.7389483522133204 2212 24 0.8507191256508335 2212 25 0.850632755173983 2213 1 -0.19034791147969798 2213 2 -0.2539959387552777 2213 3 -0.33201808074514005 2213 4 -0.291951560466714 2213 5 -0.3082624452918665 2213 6 -0.03717785494393411 2213 7 -0.12978488853409686 2213 8 -0.08387977040988659 2213 9 0.5268781398396669 2213 10 -0.10662191752280137 2213 11 0.17181422665746027 2213 12 -0.008273977465911742 2213 13 -0.2833042470495286 2213 14 -0.22503465780692977 2213 15 -0.1242948748078021 2213 16 -0.2843853726480816 2213 17 -0.4736019500917976 2213 18 -0.5664466489309419 2213 19 -0.5089450424932096 2213 20 -0.022422447142901092 2213 21 -0.3462866811194445 2213 22 -0.17285426811524474 2213 23 -0.5334206232979 2213 24 -0.37843131005203645 2213 25 -0.10562124560446051 2214 1 -1.0301524193791707 2214 2 -0.9610022503592326 2214 3 -0.8895723577184391 2214 4 -0.8189426077611088 2214 5 -0.9413213960683571 2214 6 -0.891371600791885 2214 7 -0.9111075240980568 2214 8 -0.8918802560924668 2214 9 -10.800338905478924 2214 10 -3.924062007215209 2214 11 -1.8907099480469078 2214 12 -1.2668423194564795 2214 13 -1.0291760262007763 2214 14 -1.1252054086274512 2214 15 -1.1429075734294472 2214 16 -1.4804270272240174 2214 17 -1.3196585674520662 2214 18 -1.667565566915755 2214 19 -1.9112004512236318 2214 20 -1.5318508655393153 2214 21 -2.1316295720460943 2214 22 -2.047009308498552 2214 23 -2.3831074585441847 2214 24 -2.713300368667568 2214 25 -10.406831901830358 2215 1 -0.5549647384563592 2215 2 -0.33369144250499616 2215 3 -0.15750320921027594 2215 4 -0.31104368314004205 2215 5 -0.19391757402092935 2215 6 -0.2446769063980767 2215 7 -0.27418357601895593 2215 8 -0.2750836696940695 2215 9 0.5744003295928835 2215 10 0.44319409736075244 2215 11 0.5630971700293159 2215 12 0.4108876407961972 2215 13 0.28786325362584364 2215 14 0.15433409563821954 2215 15 -0.12633964886224902 2215 16 -0.0032942078505726613 2215 17 -0.029704592420323195 2215 18 0.014505709607121275 2215 19 -0.07145425687870244 2215 20 0.14268740514282996 2215 21 0.22067889166692822 2215 22 0.23210874910281376 2215 23 0.6348508243873425 2215 24 0.698152274027457 2215 25 0.820242729640718 2216 1 -0.6117842753549354 2216 2 -0.5785240827046216 2216 3 -0.6801845192241096 2216 4 -0.45774469425519015 2216 5 -1.309648957963109 2216 6 -1.726778388402014 2216 7 -1.1392986752643104 2216 8 -2.0697256033233096 2216 9 -0.6612171257907171 2216 10 -1.1640057150711804 2216 11 -0.8502823356423477 2216 12 -1.9701714700918025 2216 13 -1.491527075936606 2216 14 -1.793300463551816 2216 15 -1.329268398977014 2216 16 -2.3716958629806086 2216 17 -2.779854780803273 2216 18 -2.1174306095503326 2216 19 -3.666221633912617 2216 20 -2.35576262492712 2216 21 -2.6419324224116316 2216 22 -2.4492071544713205 2216 23 -2.159689531388487 2216 24 -2.2996240665460626 2216 25 -2.3518772719664547 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 75595.0 3 2.0 4 1049.0 5 10770.0 6 15661.0 7 12515.0 8 9818.0 9 7801.0 10 6663.0 11 5976.0 12 5126.0 13 4934.0 14 4747.0 15 4721.0 16 5064.0 17 5255.0 18 6102.0 19 8114.0 20 11013.0 21 14677.0 22 17957.0 23 20765.0 24 23643.0 25 26473.0 26 29502.0 27 32146.0 28 35426.0 29 40822.0 30 50097.0 31 62062.0 32 76319.0 33 94080.0 34 117374.0 35 146868.0 36 193219.0 37 289341.0 38 664446.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.29356504213895 20.00483246970107 13.214837929642611 22.48676455851737 2 15.634987474913547 22.04532250868751 39.60956185370343 22.71012816269551 3 18.0036804686582 29.051687131139754 30.144779768751217 22.799852631450825 4 11.027134677153942 17.801511107657177 37.68958483630291 33.48176937888597 5 11.230044688284814 40.680762309180366 34.4038447940232 13.685348208511613 6 28.464267622264277 39.481372307582824 18.283435694267443 13.770924375885455 7 26.46292454274609 34.29117509208756 22.26615705383476 16.97974331133158 8 26.807895332018163 35.009465763809644 19.798828521004257 18.383810383167937 9 27.139763092765644 15.402622884798648 20.74378288264452 36.71383113979119 10 15.1930098807834 31.508501857066523 31.762604566824187 21.535883695325886 11 32.536243015059185 24.961972699020468 23.372646943672763 19.12913734224758 12 22.657989910930606 29.447442034668008 29.458397219068576 18.43617083533281 13 27.818919869125548 23.172914287907876 27.484009357270494 21.524156485696082 14 21.658417695681322 22.731392531170062 30.06169406336258 25.548495709786035 15 23.36333614541423 29.82516097680642 25.601889868175515 21.209613009603835 16 21.35238664177246 29.681428408237558 27.404291816559397 21.561893133430587 17 19.760115995278912 29.386703521479212 29.195734542350966 21.65744594089091 18 19.41493607886377 26.823701269058702 32.91167154766168 20.84969110441584 19 20.998817308313583 26.58645026748684 32.869277453102015 19.545454971097563 20 20.7369854239745 26.140864748042453 33.16172215469727 19.96042767328578 21 21.973574741644196 25.28682140782083 32.764286526919896 19.975317323615073 22 21.16468454431474 25.75739983501301 32.761575073293734 20.316340547378513 23 20.4343748961254 25.890910486898576 32.986031295104986 20.68868332187104 24 20.38589327589347 26.478969640421884 32.76853191737386 20.366605166310787 25 20.326757352930837 25.83691562353406 33.358623763225346 20.477703260309752 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2613.0 1 2613.0 2 3379.5 3 4146.0 4 4146.0 5 4146.0 6 12043.0 7 19940.0 8 19940.0 9 19940.0 10 19170.0 11 18400.0 12 18400.0 13 18400.0 14 17262.5 15 16125.0 16 16125.0 17 16125.0 18 27119.5 19 38114.0 20 38114.0 21 38114.0 22 56788.5 23 75463.0 24 75463.0 25 75463.0 26 111227.5 27 146992.0 28 146992.0 29 146992.0 30 167156.5 31 187321.0 32 187321.0 33 187321.0 34 201617.5 35 215914.0 36 215914.0 37 215914.0 38 219592.0 39 223270.0 40 223270.0 41 223270.0 42 230017.0 43 236764.0 44 236764.0 45 236764.0 46 243971.0 47 251178.0 48 251178.0 49 251178.0 50 237444.0 51 223710.0 52 223710.0 53 223710.0 54 201526.5 55 179343.0 56 179343.0 57 179343.0 58 152560.5 59 125778.0 60 125778.0 61 125778.0 62 105592.0 63 85406.0 64 85406.0 65 85406.0 66 64571.5 67 43737.0 68 43737.0 69 43737.0 70 32993.5 71 22250.0 72 22250.0 73 22250.0 74 15880.0 75 9510.0 76 9510.0 77 9510.0 78 7702.5 79 5895.0 80 5895.0 81 5895.0 82 4231.5 83 2568.0 84 2568.0 85 2568.0 86 1833.5 87 1099.0 88 1099.0 89 1099.0 90 830.0 91 561.0 92 561.0 93 561.0 94 301.5 95 42.0 96 42.0 97 42.0 98 23.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.027573060417771654 2 0.001404400360837266 3 9.36266907224844E-5 4 0.004400454463956767 5 0.03202032822708967 6 0.011094762850614401 7 0.01891259152594185 8 0.025840966639405695 9 0.03314384851575948 10 0.03679528945393637 11 0.039089143376637235 12 0.00786464202068869 13 0.00234066726806211 14 0.005570788097987822 15 0.005664414788710307 16 0.008005082056772417 17 0.009081789000080987 18 0.0144185103712626 19 0.01577609738673862 20 0.014371697025901355 21 0.01086069612380819 22 0.010158495943389557 23 0.0072092551856313 24 0.005243094680459126 25 0.10607904058857483 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2136143.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.5804784149326 #Duplication Level Percentage of deduplicated Percentage of total 1 76.00909475563464 27.044399552914243 2 11.065460428958252 7.8742875188767965 3 4.383839131129146 4.679372807390323 4 2.2821275944249786 3.2479676645424007 5 1.3441103828928056 2.3912045232902126 6 0.8714580644611334 1.8604136911246978 7 0.6126022757994037 1.525767743571349 8 0.4432516287742731 1.2616884007989393 9 0.341230383438394 1.0927026263204056 >10 2.1339187809103866 14.763023071311693 >50 0.2741738131602059 6.880827722210084 >100 0.216196505883014 14.806071327432987 >500 0.016378091957346037 3.8820763364698023 >1k 0.005372014162009501 3.129921715317389 >5k 0.0 0.0 >10k+ 7.861484139526098E-4 5.560275298428683 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 33385 1.562863534885071 No Hit TATCAACGCAGAGTACTTTTTTTTT 24925 1.166822633128962 No Hit GGTATCAACGCAGAGTACTTTTTTT 22813 1.0679528477260183 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 16025 0.7501838594139063 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 11624 0.5441583264790794 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 10498 0.4914464996023206 No Hit GTGGTATCAACGCAGAGTACTTTTT 4320 0.2022336519605663 No Hit ATCAACGCAGAGTACTTTTTTTTTT 4244 0.19867583771311192 No Hit GTGGTATCAACGCAGAGTACATGGG 3939 0.18439776737793304 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 3601 0.1685748566458332 No Hit GGTATCAACGCAGAGTACATGGGAT 2604 0.12190195132067469 No Hit GTATAACGCAGAGTACTTTTTTTTT 2595 0.12148063121242353 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 4.68133453612422E-5 0.0 0.0 0.0 0.0 3 4.68133453612422E-5 0.0 0.0 0.0 0.0 4 4.68133453612422E-5 0.0 0.0 0.0 0.0 5 4.68133453612422E-5 0.0 0.0 0.0 0.0 6 4.68133453612422E-5 0.0 0.0 0.0 0.0 7 4.68133453612422E-5 0.0 0.0 0.0 0.0 8 4.68133453612422E-5 0.0 0.0 0.0 0.0 9 4.68133453612422E-5 0.0 0.0 0.0 0.0 10 4.68133453612422E-5 0.0 0.0 9.36266907224844E-5 0.0 11 4.68133453612422E-5 0.0 0.0 9.36266907224844E-5 0.0 12 9.36266907224844E-5 0.0 0.0 9.36266907224844E-5 0.0 13 1.4044003608372661E-4 0.0 0.0 9.36266907224844E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGATCAC 25 0.0060422122 18.994873 14 TATCACG 215 0.0 16.78931 2 TAACGCA 355 0.0 15.52239 4 TATAACG 355 0.0 15.519845 2 CGTATCA 50 8.722513E-5 15.198745 2 TCGACCG 45 6.780056E-4 14.773098 18 ACAACGC 85 1.7180355E-8 14.529232 3 ATAACGC 400 0.0 14.487322 3 ATCACGC 250 0.0 14.439821 3 TCACGCA 260 0.0 14.25116 4 GTATCAC 305 0.0 13.706354 1 GGTATCA 5560 0.0 13.670497 1 TTGCGCG 280 0.0 12.888774 18 CGTCTTA 105 2.0025254E-8 12.663249 15 GGCGTAG 85 3.9396145E-6 12.295693 10 TTTGCGC 295 0.0 12.233985 17 ATTTGCG 305 0.0 12.144263 16 ACGATTA 55 0.0030634573 12.093876 6 CATTCCG 220 0.0 12.093309 9 CCGGCTA 55 0.0030704516 12.090194 11 >>END_MODULE