FastQCFastQC Report
Thu 26 May 2016
SRR1364285_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364285_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5629918
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT237580.42199548909948603No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN155090.2754747049601788No Hit
TATCAACGCAGAGTACTTTTTTTTT135140.24003902010650957No Hit
GGTATCAACGCAGAGTACTTTTTTT101840.18089073411015932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54200.013.138431
AGGACCG4000.012.5935055
CTATACG802.8547318E-511.8809844
CGCATAT3200.011.8750518
TGGTATC16900.011.2360372
GTATCAA136350.011.162461
TATAACG2650.010.7484362
GTGGTAT19850.010.7144291
CTCAACG3100.010.413273
GTATAAT10750.010.1571271
CAGGACC10250.09.8293414
GGACCGA3000.09.8169716
ACGCATA10400.09.6828197
ACCGAGT2750.09.6727698
GTATTAG6450.09.5683081
TAACGCA3700.09.5047874
ATAACCG1701.6550985E-89.5045335
CCGAGTG2900.09.4997019
CCTAGAC6600.09.4944523
CCCGTAC1304.275744E-69.49445153
Produced by FastQC (version 0.11.5)