##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364285_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5629918
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.470502589913387	30.0	25.0	31.0	2.0	34.0
2	25.39154264769043	30.0	25.0	31.0	2.0	34.0
3	24.765402089337712	30.0	22.0	31.0	2.0	34.0
4	28.486974055394768	35.0	27.0	37.0	2.0	37.0
5	28.976058798724956	35.0	28.0	37.0	2.0	37.0
6	29.151657626274485	35.0	29.0	37.0	2.0	37.0
7	28.67301868339823	35.0	28.0	37.0	2.0	37.0
8	28.258106245952426	35.0	27.0	37.0	2.0	37.0
9	29.255841736948923	35.0	27.0	39.0	2.0	39.0
10	28.504623335544142	35.0	25.0	39.0	2.0	39.0
11	29.171301074012092	35.0	27.0	39.0	2.0	39.0
12	28.69015463457905	35.0	25.0	39.0	2.0	39.0
13	28.311787134377447	35.0	23.0	39.0	2.0	39.0
14	28.542185694356473	34.0	19.0	40.0	2.0	41.0
15	29.235407513928266	36.0	26.0	40.0	2.0	41.0
16	29.566466332191695	36.0	27.0	40.0	2.0	41.0
17	29.72905306968947	36.0	27.0	40.0	2.0	41.0
18	29.570991620126616	36.0	27.0	40.0	2.0	41.0
19	29.538101265418074	36.0	27.0	40.0	2.0	41.0
20	29.42849291943506	36.0	26.0	40.0	2.0	41.0
21	29.318069463889174	36.0	25.0	40.0	2.0	41.0
22	29.38380665579854	36.0	26.0	40.0	2.0	41.0
23	29.3776253934782	37.0	27.0	40.0	2.0	41.0
24	29.215510421288553	37.0	26.0	40.0	2.0	41.0
25	29.026264503319588	36.0	25.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.24396925260366586
1101	2	0.3140601705585908
1101	3	1.074745426782009
1101	4	0.4064284070710755
1101	5	-0.029602067604113103
1101	6	-0.25037531483595643
1101	7	-0.01383366555387866
1101	8	0.3183759678260003
1101	9	0.22600705142289712
1101	10	0.5379343997877726
1101	11	0.012905604454335418
1101	12	0.25457865987092276
1101	13	0.16055024311183175
1101	14	0.6883797815327348
1101	15	-0.12761000306078074
1101	16	-0.5757896201300099
1101	17	-1.2971117455255232
1101	18	-6.9893899489273
1101	19	-8.508456107625125
1101	20	-9.537300790781394
1101	21	-11.232104356667737
1101	22	-11.613163544522816
1101	23	-12.899241184041959
1101	24	-12.672644945561586
1101	25	-13.138616515757112
1102	1	1.5111397320617037
1102	2	1.699612027940816
1102	3	2.5106653600629087
1102	4	1.8905205941733172
1102	5	1.266217445072897
1102	6	1.409091312315983
1102	7	1.6010223526532918
1102	8	1.8837620153272638
1102	9	2.02149980768921
1102	10	-1.6340355044270076
1102	11	0.5379270715906195
1102	12	1.5318644349485275
1102	13	2.088918398799862
1102	14	2.558836917550792
1102	15	2.1935958230820134
1102	16	1.9211269961933723
1102	17	1.5802067051931523
1102	18	1.7936057044525384
1102	19	1.501314478978621
1102	20	1.880495085248743
1102	21	1.9227616542064112
1102	22	1.7938606734465061
1102	23	1.7672088165279654
1102	24	1.9143828249338277
1102	25	2.006170104707717
1103	1	-0.4134532700632043
1103	2	-0.08844280390736614
1103	3	-2.482227320040046
1103	4	-0.3051558188981005
1103	5	-3.18924789033127
1103	6	-7.30054493666578
1103	7	-8.20181636124958
1103	8	-2.595533892621898
1103	9	-7.340376429624186
1103	10	-5.025233211946102
1103	11	-8.60028961146045
1103	12	-2.7197764076989266
1103	13	-0.5556261976967392
1103	14	0.03767867264046032
1103	15	-0.3840017298355214
1103	16	-0.4074536317742137
1103	17	-0.4094127565064447
1103	18	-0.08895200344573695
1103	19	-5.93823641813486
1103	20	-1.6664197022742897
1103	21	-6.797732941525371
1103	22	-2.6702704931381867
1103	23	-1.7026277853917549
1103	24	-6.6405768453012115
1103	25	-3.519644672021645
1104	1	1.163845707131408
1104	2	1.153233211395193
1104	3	2.1024975408764597
1104	4	1.4730146646611182
1104	5	1.1461741392606157
1104	6	0.966026247885825
1104	7	1.0367290945422063
1104	8	1.3697477478425242
1104	9	1.566068891620077
1104	10	1.765276485607945
1104	11	1.4719887358992274
1104	12	1.735849407517474
1104	13	1.9447058547110316
1104	14	2.350891256657686
1104	15	1.4473661157268012
1104	16	0.5995304151008476
1104	17	0.503198615790474
1104	18	0.48084217375898675
1104	19	0.9809771439813915
1104	20	-9.195654088088727
1104	21	-3.6188544393749886
1104	22	-1.394781041976838
1104	23	-0.3134440696479821
1104	24	0.24559851975614677
1104	25	0.7383035607059405
1105	1	1.110230899956452
1105	2	1.0848965537084219
1105	3	1.900738517007266
1105	4	1.2294270516543655
1105	5	1.060437450836261
1105	6	1.0061174107371293
1105	7	1.2264401312734847
1105	8	1.470483791070734
1105	9	1.380928748155366
1105	10	1.9438755592568349
1105	11	1.4677281363457944
1105	12	1.6435563670887596
1105	13	1.7333035167227138
1105	14	2.3365079326031406
1105	15	1.7377810724460296
1105	16	1.177496710214033
1105	17	0.4736420370847725
1105	18	1.094062545915584
1105	19	1.101592476573174
1105	20	1.0223566306278045
1105	21	0.9568671993579407
1105	22	0.9559010439339772
1105	23	0.9604780614059116
1105	24	0.8453181375628667
1105	25	0.869468573101237
1106	1	0.8362448526492798
1106	2	1.1096428311154298
1106	3	1.7075921261038722
1106	4	1.2480260179831006
1106	5	0.6494445962994391
1106	6	0.41108213877761557
1106	7	0.952508207884609
1106	8	0.8376455154549483
1106	9	1.1841281735352176
1106	10	-2.5592712673413693
1106	11	-0.3644374297579631
1106	12	0.4987102619102295
1106	13	1.3255458224595245
1106	14	0.4219873496142661
1106	15	1.129945521117527
1106	16	0.7373146108097757
1106	17	-5.866971837106526
1106	18	-1.9374493168553215
1106	19	-0.4898311024954438
1106	20	0.2903921873113511
1106	21	0.5880602389346627
1106	22	0.6631042861696841
1106	23	0.1630873449222463
1106	24	0.5682093149395513
1106	25	0.6682529804809896
1107	1	0.6411653645455573
1107	2	1.0465944275854753
1107	3	1.5449296059866988
1107	4	1.050072164509963
1107	5	0.4128102679774166
1107	6	0.6930113241605973
1107	7	0.6037091366556595
1107	8	0.7899411095844755
1107	9	0.9670178244567396
1107	10	-2.1539293655859275
1107	11	-0.08198483409664448
1107	12	0.2841167622300027
1107	13	1.1692197886912261
1107	14	1.6296491989343878
1107	15	1.2838138379988244
1107	16	-5.436712194671472
1107	17	-1.9625602150222718
1107	18	-0.18840343810849092
1107	19	0.6254423267753353
1107	20	0.9951625107109621
1107	21	1.17038671666824
1107	22	0.7422260242506447
1107	23	0.5161081002989967
1107	24	0.6524618692215789
1107	25	0.9043031900444554
1108	1	-0.605476803816817
1108	2	-3.0796879574160343
1108	3	-0.1837195915786829
1108	4	-0.8428667107747074
1108	5	-1.1791127899936917
1108	6	-0.770697978339367
1108	7	-0.4630121075625482
1108	8	-0.1846731519207303
1108	9	-0.7530246699783838
1108	10	-3.8465357944894265
1108	11	-1.8295057599547278
1108	12	-6.155460157303725
1108	13	-2.1869473675130493
1108	14	-6.356696665276235
1108	15	-3.1350278967424927
1108	16	-7.817064222556905
1108	17	-3.7108176432466315
1108	18	-2.3568398314267824
1108	19	-0.9534716710008553
1108	20	-0.8630900542732789
1108	21	-0.42441309780021896
1108	22	-0.47333050609533345
1108	23	-0.5282435248158919
1108	24	-0.24283176764649284
1108	25	-0.5111465418346199
1109	1	-8.273212077349854
1109	2	-5.941056469028428
1109	3	-5.238630698394523
1109	4	-4.873422834822168
1109	5	-2.5179175420806885
1109	6	-1.5774246272185515
1109	7	-1.199151011611221
1109	8	-0.7719744008342779
1109	9	-5.688270757490766
1109	10	-1.7342768034741276
1109	11	-1.3138303852553967
1109	12	-6.0463588229900616
1109	13	-7.903931884320194
1109	14	-8.065319139908862
1109	15	-4.022636731359356
1109	16	-2.330722872224399
1109	17	-1.898133830864122
1109	18	-1.3425640522138806
1109	19	-1.050174512702391
1109	20	-0.7745600732735447
1109	21	-0.6877071230766347
1109	22	-0.8677947214249073
1109	23	-1.1631035431592274
1109	24	-0.8247558727139044
1109	25	-0.8001738269194121
1110	1	-2.3846621450947687
1110	2	-2.2685766254189303
1110	3	-1.5780678206837138
1110	4	-2.4192414597117917
1110	5	-5.410386920164143
1110	6	-3.9976096751361823
1110	7	-8.785846274935398
1110	8	-9.667243155239216
1110	9	-11.189223401365165
1110	10	-9.056790101831236
1110	11	-11.716781453478273
1110	12	-6.647714088359752
1110	13	-4.919477975165215
1110	14	-4.432729443717967
1110	15	-9.7658268231846
1110	16	-11.26320531829175
1110	17	-12.034917851165318
1110	18	-8.948251511202386
1110	19	-11.911617998355453
1110	20	-12.88742649326366
1110	21	-13.969895615453558
1110	22	-14.700834164357158
1110	23	-15.278698809825086
1110	24	-15.724668533418209
1110	25	-16.206184415986254
1111	1	-0.6306096017744771
1111	2	0.008337851942723518
1111	3	-2.7039482007843283
1111	4	-3.0701078655250953
1111	5	-1.092667523853354
1111	6	-0.555463224605159
1111	7	-0.09160368130767438
1111	8	0.12001229473283459
1111	9	-4.2270098742550495
1111	10	-4.304079090092834
1111	11	-1.4327121410132087
1111	12	-5.773035496967914
1111	13	-1.2863868628119448
1111	14	0.028645626153465997
1111	15	0.06882940934754345
1111	16	-0.08165137754273033
1111	17	-0.011217459885273229
1111	18	0.21429739880320398
1111	19	0.3154362218793132
1111	20	0.35807378622208574
1111	21	0.5614626852731028
1111	22	0.1447779094270807
1111	23	0.10855664014696487
1111	24	0.2638166538372424
1111	25	0.5509983676917791
1112	1	-0.2387349850741316
1112	2	0.28114714856338097
1112	3	-2.61686858845302
1112	4	-0.4446892657017578
1112	5	-0.3851376635511805
1112	6	-0.14449923716005486
1112	7	0.26561232532340284
1112	8	-3.442433142354215
1112	9	-0.849961091072295
1112	10	-3.0528796038410704
1112	11	-0.6557889418442961
1112	12	0.47609835999920236
1112	13	0.8760573604798338
1112	14	1.5458416764886813
1112	15	1.068688634122779
1112	16	0.7819018041462797
1112	17	0.5280224139651111
1112	18	0.6054379321448486
1112	19	0.7712747961178472
1112	20	0.7774326195170644
1112	21	0.7211228626398771
1112	22	0.6691855554363286
1112	23	0.4673923001506459
1112	24	0.634242056545105
1112	25	0.7540765149596815
1113	1	-1.0098979886460029
1113	2	-0.6131944226997739
1113	3	-2.982248987686674
1113	4	-1.41046462838991
1113	5	-1.6353803503038762
1113	6	-1.4872463637458893
1113	7	-1.064992170510891
1113	8	-8.212781912645838
1113	9	-9.240128912593164
1113	10	-3.5367810558803647
1113	11	-9.212765016152233
1113	12	-7.844828068595703
1113	13	-3.2123039387509955
1113	14	-1.4060985057126025
1113	15	-7.26489037651141
1113	16	-3.2940711367708424
1113	17	-2.340532741141807
1113	18	-1.4559516886325383
1113	19	-1.1700304942171336
1113	20	-6.432631022363434
1113	21	-2.6264281900381015
1113	22	-2.141255659484166
1113	23	-1.9890777834610596
1113	24	-7.4575157514745705
1113	25	-4.655576863922466
1114	1	-4.148907600124183
1114	2	-3.7425874578329683
1114	3	-0.7218764473784951
1114	4	-0.5425325177452578
1114	5	-0.5460116299573841
1114	6	-0.6425557521322851
1114	7	-0.3968256531264416
1114	8	-0.02702637633462146
1114	9	-0.43570732773077836
1114	10	-3.0901286601185554
1114	11	-1.1696654678794296
1114	12	-4.636589274067433
1114	13	-1.259953278385069
1114	14	-5.69418734882689
1114	15	-2.010608560590242
1114	16	-1.0789556093611203
1114	17	-0.965955987907293
1114	18	-0.5561560409417368
1114	19	-0.3270566776053876
1114	20	-0.07095940998085126
1114	21	0.07248249524251449
1114	22	-2.1722545067248689E-4
1114	23	-0.38271920242615565
1114	24	-0.27642033479503425
1114	25	-0.07292059672234075
1115	1	1.0380969489013907
1115	2	1.110411341671064
1115	3	1.8588253687588363
1115	4	0.7591978883114763
1115	5	0.632918645067889
1115	6	0.10508620956635539
1115	7	-1.8353601422773949
1115	8	0.31327167565182634
1115	9	0.6527305773427514
1115	10	1.3334138260813724
1115	11	0.49897940072774105
1115	12	1.2433093173443766
1115	13	1.4158676158764862
1115	14	2.095893377252594
1115	15	1.149597309450204
1115	16	0.8168163147082126
1115	17	0.44938474785875826
1115	18	0.820081072968339
1115	19	1.1008363862686927
1115	20	1.046105935858666
1115	21	1.2094881236819717
1115	22	0.7442548144444672
1115	23	0.8139414781865391
1115	24	0.6654882731083411
1115	25	0.9158919236339962
1116	1	0.6683764022328944
1116	2	0.8568708171284705
1116	3	1.6885415574673601
1116	4	1.0480806279626549
1116	5	0.24925704349829658
1116	6	-0.6884414931721778
1116	7	-4.178476342545071
1116	8	-0.4058449847110488
1116	9	-0.5374002443461734
1116	10	0.23182530538850088
1116	11	-1.530348432204562
1116	12	0.1501869003412004
1116	13	-5.798099146219435
1116	14	-0.9714131570871203
1116	15	-0.6077174491479944
1116	16	-0.158500202289364
1116	17	0.18504963141050368
1116	18	0.39676683873332763
1116	19	0.6564847531748583
1116	20	0.6971477301481919
1116	21	0.813355478190207
1116	22	0.605386137673495
1116	23	0.4280921988793409
1116	24	0.6032831181117828
1116	25	0.5406819030057903
1201	1	1.934682558310218
1201	2	2.0419046140800106
1201	3	2.704925613240185
1201	4	2.275661510531357
1201	5	1.7467694088714616
1201	6	1.6170884174411384
1201	7	2.039838917553695
1201	8	2.0626344666524687
1201	9	2.3466402603648397
1201	10	2.6568597841254302
1201	11	2.2051485402732496
1201	12	2.5589965639235928
1201	13	2.6797252870359003
1201	14	3.222981312261844
1201	15	2.718095725902465
1201	16	2.234898129905549
1201	17	2.053105076365039
1201	18	2.0785100024744523
1201	19	2.1272906356808257
1201	20	2.2816533024393237
1201	21	2.146709313949845
1201	22	1.237314530131627
1201	23	0.6659629473268964
1201	24	1.1946448201939788
1201	25	1.2921764702520768
1202	1	2.002404861118027
1202	2	2.214219286899297
1202	3	2.945408228530809
1202	4	2.4916877419576373
1202	5	1.8911706014760483
1202	6	1.7757462213873012
1202	7	2.082397299223082
1202	8	2.358536821985602
1202	9	2.4271926859288904
1202	10	-1.160474128796217
1202	11	1.160700590025467
1202	12	2.1877981866005847
1202	13	2.7319823809597743
1202	14	3.234621461778815
1202	15	2.7909697503980517
1202	16	2.401047107461906
1202	17	1.9543326813389967
1202	18	2.1106550041678
1202	19	2.30366194777282
1202	20	2.281666086693079
1202	21	2.464355583040323
1202	22	2.2469967465748155
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2211	19	0.6874435944847228
2211	20	0.9167919455524114
2211	21	0.9562496156112452
2211	22	0.7615478853850703
2211	23	0.6616415661136692
2211	24	0.9040246636642166
2211	25	0.9167485843192047
2212	1	0.8584010872911065
2212	2	0.9564068630091285
2212	3	1.6192360334634657
2212	4	1.095532864789405
2212	5	0.7030893792020834
2212	6	0.6156928291046029
2212	7	0.9007866531401518
2212	8	1.1550166971286693
2212	9	1.0168292822450802
2212	10	1.7294680829078075
2212	11	0.9336548441467514
2212	12	1.2290757722486916
2212	13	1.3362215615999524
2212	14	-4.308562884052954
2212	15	-0.9628229502473182
2212	16	-0.29975208219221017
2212	17	0.014475533761046933
2212	18	0.3967460133186371
2212	19	0.9288904782156777
2212	20	1.1227141876358928
2212	21	1.1433497732682056
2212	22	0.9831374656069087
2212	23	0.9575103828458396
2212	24	0.9253863080077132
2212	25	0.9758414833613784
2213	1	0.7910356967111447
2213	2	0.8488128870480658
2213	3	1.4887120229453004
2213	4	0.911532910464576
2213	5	0.4813326042603272
2213	6	0.22537864820829512
2213	7	0.3704831128085999
2213	8	-5.077812391825663
2213	9	-1.659305283311312
2213	10	0.1972507619299364
2213	11	0.14318149784617162
2213	12	0.7982876477524918
2213	13	-7.615685602764696
2213	14	-2.026067544081233
2213	15	-0.7662876294937178
2213	16	-0.2543550686685805
2213	17	-0.02243232543375484
2213	18	0.29276112731312764
2213	19	0.5861457305040823
2213	20	0.8301371755922169
2213	21	0.8362604069828095
2213	22	0.44442942480394976
2213	23	0.3891013449187035
2213	24	0.4893044739414556
2213	25	0.5803490819788877
2214	1	-0.9089085716364522
2214	2	-0.6049092226971062
2214	3	-6.765601972742438
2214	4	-2.4815122056656236
2214	5	-1.4798348296704127
2214	6	-0.9955566040738155
2214	7	-0.5472568551113177
2214	8	-5.984763724255586
2214	9	-1.9003106517973798
2214	10	-0.22454111938977306
2214	11	-0.5923835350184028
2214	12	-0.209871191982117
2214	13	-6.3444972654714284
2214	14	-1.6004027854095568
2214	15	-0.7834222843220431
2214	16	-0.7504891788466672
2214	17	-0.5721927230282091
2214	18	-0.5942262439130701
2214	19	-0.17625181705476578
2214	20	0.03595177885124201
2214	21	0.13895448579642533
2214	22	0.01589493190442326
2214	23	-0.06486841176463187
2214	24	0.10024805070103682
2214	25	0.19649283406114648
2215	1	0.14771338966146175
2215	2	0.43166271666896705
2215	3	-2.621524512937942
2215	4	-0.7750966438768572
2215	5	-0.292428012891893
2215	6	-0.7858295017126835
2215	7	-0.09425994182457842
2215	8	0.3427795268927305
2215	9	0.589721334278952
2215	10	1.2060943720057864
2215	11	0.4873226686530181
2215	12	0.28422814095944915
2215	13	0.9453649012533525
2215	14	0.9434420623422994
2215	15	0.5606804758926529
2215	16	0.29498305920242274
2215	17	0.20250132172574808
2215	18	0.28700901124899403
2215	19	0.3623212163294056
2215	20	0.5919161688061081
2215	21	0.7645515154207985
2215	22	0.6049075367737906
2215	23	0.4523141927154981
2215	24	0.28579926323775595
2215	25	0.37786153161517433
2216	1	0.3489467741836805
2216	2	0.17438304767282986
2216	3	1.3361783580243305
2216	4	-0.015396552293299948
2216	5	-0.24101747467253176
2216	6	-0.5014629523032568
2216	7	-1.138745320943702
2216	8	-6.079503742229981
2216	9	-1.8457437775979066
2216	10	-0.5339826965878522
2216	11	-1.2091333505490667
2216	12	0.05089116259701143
2216	13	0.6641836142340765
2216	14	1.4557463247195948
2216	15	1.0381123750390984
2216	16	0.31245155303237127
2216	17	0.274576841798325
2216	18	0.34232360455443
2216	19	0.555053582972846
2216	20	0.5342540975174366
2216	21	0.4776930075266712
2216	22	0.17364605030978808
2216	23	-0.03126252995255285
2216	24	0.14203064299396928
2216	25	0.12383650382402678
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	635172.0
3	14.0
4	4390.0
5	27965.0
6	38899.0
7	35499.0
8	30887.0
9	26334.0
10	23238.0
11	21363.0
12	19672.0
13	18599.0
14	18785.0
15	19385.0
16	19974.0
17	21773.0
18	25194.0
19	32089.0
20	43779.0
21	55171.0
22	67487.0
23	79616.0
24	90664.0
25	101598.0
26	113096.0
27	124088.0
28	134747.0
29	155717.0
30	175747.0
31	196797.0
32	240157.0
33	297172.0
34	365199.0
35	445987.0
36	543397.0
37	622337.0
38	757930.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.86469421624288	17.741806130217945	13.366847120953324	23.026652532585853
2	14.269666545890642	19.650496877794208	42.41439531400905	23.6654412623061
3	16.951074548273706	25.79234912289176	29.943919329962277	27.312656998872253
4	10.766306683452852	16.02580956117365	37.99249184828478	35.215391907088716
5	12.907098623485243	38.65352185988142	33.93846861396622	14.500910902667108
6	31.844183636438462	36.792189798486355	17.633152202476044	13.730474362599141
7	29.135962384265962	32.242467543690076	20.51457939312736	18.106990678916606
8	25.90268948550708	35.49794108759607	20.14373902483523	18.455630402061622
9	26.671723072855364	16.369731974838412	19.705303097520936	37.25324185478529
10	13.41663261168888	30.641078490673873	33.10734265752976	22.83494624010748
11	34.46020692013126	24.33794306017232	22.048312958992796	19.153537060703616
12	22.836871067641642	26.97740812574852	29.80419853026854	20.3815222763413
13	27.76587801623027	22.51171413397716	26.631476311358572	23.090931538433992
14	21.085954034534407	21.891259214018582	28.85039656825968	28.17239018318733
15	23.56581029636105	29.718304414230275	23.70883065182044	23.007054637588237
16	22.64455394936592	29.788690024958402	25.288552040205676	22.27820398547
17	21.734009082314284	29.121143307703257	26.399833824040535	22.74501378594192
18	21.32084432962105	28.197563696519918	28.772702056452776	21.708889917406253
19	22.66645715942023	27.341586422362692	28.60479397474262	21.387162443474462
20	22.675288892030473	26.976494868326494	28.842114958482874	21.50610128116016
21	23.550914861454675	26.333383513164186	28.42962367799157	21.686077947389563
22	23.0228029208576	26.75297687924605	28.202217384650552	22.0220028152458
23	22.589029084253443	26.905954061067348	28.057936913154848	22.447079941524365
24	22.344934921115936	26.9860332354019	28.467422076634506	22.201609766847653
25	22.396301050060703	26.530346121883287	28.65180899209671	22.4215438359593
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	20243.0
1	20243.0
2	14415.5
3	8588.0
4	8588.0
5	8588.0
6	13864.0
7	19140.0
8	19140.0
9	19140.0
10	23290.0
11	27440.0
12	27440.0
13	27440.0
14	35906.5
15	44373.0
16	44373.0
17	44373.0
18	66400.5
19	88428.0
20	88428.0
21	88428.0
22	123360.5
23	158293.0
24	158293.0
25	158293.0
26	213473.0
27	268653.0
28	268653.0
29	268653.0
30	325297.0
31	381941.0
32	381941.0
33	381941.0
34	427000.5
35	472060.0
36	472060.0
37	472060.0
38	519167.0
39	566274.0
40	566274.0
41	566274.0
42	609462.0
43	652650.0
44	652650.0
45	652650.0
46	680592.5
47	708535.0
48	708535.0
49	708535.0
50	692702.0
51	676869.0
52	676869.0
53	676869.0
54	620789.5
55	564710.0
56	564710.0
57	564710.0
58	491185.5
59	417661.0
60	417661.0
61	417661.0
62	344270.5
63	270880.0
64	270880.0
65	270880.0
66	211194.5
67	151509.0
68	151509.0
69	151509.0
70	112618.0
71	73727.0
72	73727.0
73	73727.0
74	53130.0
75	32533.0
76	32533.0
77	32533.0
78	23996.5
79	15460.0
80	15460.0
81	15460.0
82	10772.0
83	6084.0
84	6084.0
85	6084.0
86	4397.0
87	2710.0
88	2710.0
89	2710.0
90	1826.5
91	943.0
92	943.0
93	943.0
94	543.5
95	144.0
96	144.0
97	144.0
98	107.0
99	70.0
100	70.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.33559991459911137
2	0.33714523017919623
3	0.34826439745658816
4	0.35078663667925536
5	0.3569856612476416
6	0.2992761173430945
7	0.3085835353196974
8	0.3063277298177345
9	0.30615010733726494
10	0.42251059429284765
11	0.3058836736165607
12	0.30536856842319904
13	0.2988853478860616
14	0.3044271692767106
15	0.2971801720735542
16	0.3015852095891983
17	0.2946934573469809
18	0.2959190524622206
19	0.2974643680423054
20	0.4911616829943171
21	0.2979261864915262
22	0.30034185222591164
23	0.29556380750128153
24	0.2985301029251225
25	0.29188702215556245
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	5629918.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.07474768017372
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.05901894046391	59.73611224075429
2	5.5514725540893695	7.447272416379079
3	2.0578802740801554	4.140954004197995
4	1.0188225923875598	2.733490732610242
5	0.5545587301232222	1.859844344842634
6	0.3713973330500179	1.4946829442051635
7	0.24265604737494237	1.1393265205519778
8	0.18155562521123705	0.974223820076793
9	0.1366998668131069	0.8252198166962247
>10	0.7240041369915503	8.8744713852872
>50	0.06068492014867767	2.8347866795741266
>100	0.03809180369326356	4.766777916868192
>500	0.0023152667751578466	1.0562226025137902
>1k	6.8405089812754E-4	0.8128092206869773
>5k	5.261929984382193E-5	0.19265373240929215
>10k+	1.0523859968764386E-4	1.1111516223459814
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	23758	0.42199548909948603	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	15509	0.2754747049601788	No Hit
TATCAACGCAGAGTACTTTTTTTTT	13514	0.24003902010650957	No Hit
GGTATCAACGCAGAGTACTTTTTTT	10184	0.18089073411015932	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	1.7762248046952018E-5	0.0	0.0	0.0	0.0
7	3.5524496093904035E-5	0.0	0.0	0.0	1.7762248046952018E-5
8	3.5524496093904035E-5	0.0	0.0	0.0	1.7762248046952018E-5
9	3.5524496093904035E-5	0.0	0.0	0.0	1.7762248046952018E-5
10	3.5524496093904035E-5	0.0	0.0	0.0	3.5524496093904035E-5
11	3.5524496093904035E-5	0.0	0.0	1.7762248046952018E-5	3.5524496093904035E-5
12	3.5524496093904035E-5	0.0	0.0	3.5524496093904035E-5	3.5524496093904035E-5
13	7.104899218780807E-5	0.0	0.0	5.328674414085605E-5	3.5524496093904035E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5420	0.0	13.13843	1
AGGACCG	400	0.0	12.593505	5
CTATACG	80	2.8547318E-5	11.880984	4
CGCATAT	320	0.0	11.875051	8
TGGTATC	1690	0.0	11.236037	2
GTATCAA	13635	0.0	11.16246	1
TATAACG	265	0.0	10.748436	2
GTGGTAT	1985	0.0	10.714429	1
CTCAACG	310	0.0	10.41327	3
GTATAAT	1075	0.0	10.157127	1
CAGGACC	1025	0.0	9.829341	4
GGACCGA	300	0.0	9.816971	6
ACGCATA	1040	0.0	9.682819	7
ACCGAGT	275	0.0	9.672769	8
GTATTAG	645	0.0	9.568308	1
TAACGCA	370	0.0	9.504787	4
ATAACCG	170	1.6550985E-8	9.504533	5
CCGAGTG	290	0.0	9.499701	9
CCTAGAC	660	0.0	9.494452	3
CCCGTAC	130	4.275744E-6	9.4944515	3
>>END_MODULE