FastQCFastQC Report
Thu 26 May 2016
SRR1364285_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364285_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5629918
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT474080.8420726554099012No Hit
TATCAACGCAGAGTACTTTTTTTTT338910.6019803485592508No Hit
GGTATCAACGCAGAGTACTTTTTTT318790.5662427054887833No Hit
ACGCAGAGTACTTTTTTTTTTTTTT160020.28423149324732616No Hit
GTACTTTTTTTTTTTTTTTTTTTTT95730.17003800055347165No Hit
GAGTACTTTTTTTTTTTTTTTTTTT91100.16181407970773287No Hit
GTGGTATCAACGCAGAGTACATGGG83860.14895421212173962No Hit
GTGGTATCAACGCAGAGTACTTTTT56440.10025012797699717No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG3950.014.1905772
GGACCGA5200.014.0683796
GGTATCA85150.013.5240131
ACGACCG500.001497029713.3039695
GTGGTAT27600.012.7373831
TGGTATC25800.012.66729452
AGGACCG6550.012.4770065
CGAGTGG5150.012.35928610
TAACGCA4700.012.3316044
ATAACGC4700.012.3307273
ACCGAGT5600.012.0451338
GCGTATC658.019243E-411.694031
TATCACG1900.011.500572
TTGCGCG4700.011.11441118
GCGCGGT1053.4769528E-610.85555811
ATTTGCG5050.010.72063716
CGCGCCA1701.4006218E-1010.617702510
ATCGGAC909.516969E-510.556363
CGTATCA909.519541E-510.5560792
GACCGAG6750.010.27485757
Produced by FastQC (version 0.11.5)