##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364285_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5629918
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.79042980732579	31.0	30.0	34.0	26.0	34.0
2	29.84331192745614	31.0	30.0	34.0	26.0	34.0
3	29.880204294272136	31.0	30.0	34.0	26.0	34.0
4	33.24448206883297	35.0	33.0	37.0	27.0	37.0
5	32.80710020998529	35.0	33.0	37.0	26.0	37.0
6	32.69169106903511	35.0	33.0	37.0	25.0	37.0
7	32.423866031441314	35.0	33.0	37.0	23.0	37.0
8	32.32487418111596	35.0	33.0	37.0	23.0	37.0
9	33.08798867052771	37.0	32.0	39.0	17.0	39.0
10	33.22885573111367	37.0	32.0	39.0	18.0	39.0
11	33.15883055490329	37.0	33.0	39.0	17.0	39.0
12	33.11277002613537	37.0	32.0	39.0	17.0	39.0
13	33.16220627014461	37.0	33.0	39.0	17.0	39.0
14	34.10425036385965	38.0	33.0	41.0	17.0	41.0
15	34.13789401550786	38.0	33.0	41.0	17.0	41.0
16	34.10172581554474	38.0	33.0	41.0	17.0	41.0
17	34.07836988034284	38.0	33.0	41.0	17.0	41.0
18	34.03890198756003	38.0	33.0	41.0	17.0	41.0
19	33.954998101215686	39.0	33.0	41.0	18.0	41.0
20	33.85988694684363	39.0	33.0	41.0	17.0	41.0
21	33.825764602610555	39.0	33.0	41.0	16.0	41.0
22	33.756242808509825	39.0	33.0	41.0	11.0	41.0
23	33.50446098859699	39.0	33.0	41.0	9.0	41.0
24	33.42032548253811	39.0	33.0	41.0	2.0	41.0
25	33.25008925529644	38.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.10754451047025526
1101	2	0.2923507883378207
1101	3	-1.6781211944114318
1101	4	-0.6337648173001327
1101	5	0.1514418797035404
1101	6	0.5126765785389154
1101	7	0.5809776916731977
1101	8	0.7646486336071732
1101	9	1.4229243155627458
1101	10	1.0798869348684548
1101	11	0.9878201646168918
1101	12	1.0821805763703196
1101	13	0.888988196177074
1101	14	0.9623665866011493
1101	15	0.933461613354055
1101	16	0.9048390280894694
1101	17	0.8806932595049304
1101	18	0.9173879869321269
1101	19	0.9839761573934425
1101	20	1.2067523804512703
1101	21	0.9933456402033869
1101	22	1.0872619681401758
1101	23	1.3870588087765796
1101	24	1.2876755629835799
1101	25	1.4173422499597237
1102	1	0.6381450583571677
1102	2	0.6138029166239072
1102	3	0.6793362607640496
1102	4	0.7307333836398584
1102	5	0.7609076519695179
1102	6	0.8803919053143261
1102	7	1.0036428081466582
1102	8	1.0484713416579154
1102	9	1.656645957095762
1102	10	1.2775436924569945
1102	11	1.254087599541073
1102	12	1.4094300662883654
1102	13	1.2262015719127533
1102	14	1.2545246227191313
1102	15	1.2535574175737736
1102	16	1.1944807548831164
1102	17	1.1709630425158792
1102	18	1.2177400099722533
1102	19	1.2207856470075313
1102	20	1.409444986257654
1102	21	1.5309410549750595
1102	22	1.4811323408324597
1102	23	1.8386343635975635
1102	24	1.7850775645565662
1102	25	1.6352121211189115
1103	1	-2.4067934101782065
1103	2	-2.321110299892176
1103	3	-2.3107289084477465
1103	4	-2.18682839289065
1103	5	-4.753023185375891
1103	6	-9.092940143981512
1103	7	-10.054551020760517
1103	8	-10.384600724515685
1103	9	-11.31709314932186
1103	10	-9.077893829316167
1103	11	-11.802884411673137
1103	12	-10.918998329985428
1103	13	-11.540348475710818
1103	14	-11.667770340083642
1103	15	-11.96374865021436
1103	16	-6.522459082143225
1103	17	-4.601114226379458
1103	18	-3.937927647514627
1103	19	-3.5388349500742144
1103	20	-8.035229306643796
1103	21	-4.924667722067557
1103	22	-10.52918313382299
1103	23	-11.942346154149622
1103	24	-12.682591900924042
1103	25	-13.323206737513946
1104	1	0.23525268043540848
1104	2	0.44346589254355706
1104	3	0.4444101493425663
1104	4	0.5774107131191357
1104	5	0.5461491149283546
1104	6	0.6209188079701775
1104	7	0.49224142067189547
1104	8	0.7015748975979221
1104	9	1.147384397778879
1104	10	0.9637875841675907
1104	11	0.9132760933909765
1104	12	0.7881075829723301
1104	13	0.7142411886144231
1104	14	0.7480148414377013
1104	15	0.7089327613414795
1104	16	0.3496655884231288
1104	17	0.33589246929314953
1104	18	0.5893182796968972
1104	19	0.760189714258523
1104	20	1.0097204913421294
1104	21	0.8363560815551807
1104	22	0.6178428860530261
1104	23	1.0937774033631769
1104	24	1.1803688429453274
1104	25	1.255597860965267
1105	1	0.26664314841346126
1105	2	0.30139514000769196
1105	3	-0.05271708566917965
1105	4	0.23026562112988813
1105	5	0.2873691811747108
1105	6	0.4363625187200526
1105	7	0.529850540261279
1105	8	0.3378727068155811
1105	9	0.5927641171955003
1105	10	0.20933626036966047
1105	11	0.7480928626816947
1105	12	0.8797314047732172
1105	13	0.6967623087122377
1105	14	0.5473025075856981
1105	15	0.43776514151436174
1105	16	0.644534123962444
1105	17	0.6713153099295681
1105	18	0.61628188270668
1105	19	0.7093498167677694
1105	20	0.900485392858279
1105	21	0.8294711691589569
1105	22	0.6098425471002145
1105	23	1.0462766174363267
1105	24	0.9917232159129341
1105	25	1.0728028872464321
1106	1	-0.1627786545331773
1106	2	0.07154324306470983
1106	3	-0.07763174253139127
1106	4	-1.4588060538616112
1106	5	-0.36756635831725504
1106	6	-0.11990825810932648
1106	7	0.1721221069120915
1106	8	0.24169633362357956
1106	9	0.7694385817206353
1106	10	0.3422641667779871
1106	11	0.47258516046171195
1106	12	0.62090775536808
1106	13	0.44875250622071405
1106	14	-0.141714278871099
1106	15	0.443046224130363
1106	16	0.2517434681101278
1106	17	0.2510747203742767
1106	18	0.23311909911028295
1106	19	-1.151762037341129
1106	20	0.13970291228455523
1106	21	-0.2039682369503737
1106	22	0.4281558308943261
1106	23	0.2865678221398582
1106	24	0.42403010770506455
1106	25	0.7406433766315175
1107	1	0.08912964185243766
1107	2	-0.01745251986860552
1107	3	-0.12477789169833642
1107	4	0.02679458697856063
1107	5	0.16594809022021906
1107	6	0.08941231876536193
1107	7	-0.0278314760870515
1107	8	-0.15247530205981263
1107	9	0.7691545166797198
1107	10	0.4768121950387467
1107	11	0.45377066359452556
1107	12	0.29537754283047946
1107	13	0.1816431539089436
1107	14	0.3648576599371225
1107	15	0.22788282982184427
1107	16	0.08593967813371961
1107	17	-0.0494430056398798
1107	18	0.0352954853299039
1107	19	0.24942462689019607
1107	20	0.26098987549200814
1107	21	0.22547171216603346
1107	22	0.23874898251439447
1107	23	0.4479050870443473
1107	24	0.6513898181793536
1107	25	0.2098461410696828
1108	1	-0.11464890290329777
1108	2	-0.046746953708190375
1108	3	0.0385880917580792
1108	4	-0.15105897807637803
1108	5	0.12147204542699086
1108	6	0.09234975864436024
1108	7	-0.2970008903772623
1108	8	0.007659518953673228
1108	9	0.37672547820870506
1108	10	0.19317833703250642
1108	11	0.22727827017339308
1108	12	0.2989720557244908
1108	13	-0.20441921768116345
1108	14	-0.0035856426099698524
1108	15	-0.30376127740938585
1108	16	-0.8835333137444508
1108	17	-0.8880056075376146
1108	18	-0.8662629474641932
1108	19	-0.8063675563618347
1108	20	-0.1619986284082202
1108	21	-0.3955834931076865
1108	22	-0.29104511175533787
1108	23	-0.691062267117367
1108	24	-0.3089666337549062
1108	25	-0.5526228209606288
1109	1	0.21386030287561297
1109	2	0.20787567504891058
1109	3	0.13097371118685786
1109	4	0.20642594425498828
1109	5	0.09658031155194635
1109	6	0.24605323074025165
1109	7	0.35179570700918816
1109	8	0.32526473417207313
1109	9	1.1864913479760588
1109	10	0.50588504700562
1109	11	0.4276702453416732
1109	12	0.5393931250451303
1109	13	0.582272828990753
1109	14	0.1909641307512544
1109	15	-0.049344095310061675
1109	16	0.36296540778042186
1109	17	0.27120749860108617
1109	18	0.1564478303843302
1109	19	0.40658856364301954
1109	20	0.6691404840874853
1109	21	0.6790629680933691
1109	22	0.3360878382881154
1109	23	0.6534474591474648
1109	24	0.45164047769982574
1109	25	0.4788187292283652
1110	1	0.015448827454157765
1110	2	0.09489643547291848
1110	3	-0.004530244144028472
1110	4	0.12541010217253756
1110	5	0.058319674891521345
1110	6	0.21900612422783183
1110	7	0.1860919370907652
1110	8	0.10719490475964477
1110	9	-3.2814107013835567
1110	10	-1.2875913025257333
1110	11	-0.38725159738076087
1110	12	0.0022415582034867043
1110	13	-0.2911299757904757
1110	14	-0.016125782142353273
1110	15	0.12269978597735332
1110	16	-0.2533786857541429
1110	17	-0.11854064018586286
1110	18	-0.005058843350489894
1110	19	-0.18464391280321735
1110	20	0.20970301750425335
1110	21	-0.005467166748374552
1110	22	0.2963176368356031
1110	23	0.40385652349804957
1110	24	0.5690405666086988
1110	25	0.6320519919814203
1111	1	0.008517580947728476
1111	2	0.08897133765290732
1111	3	0.05807456301080549
1111	4	-0.11908402862678713
1111	5	0.052394577071510184
1111	6	-0.04125100265869719
1111	7	0.11733844351893907
1111	8	0.17415968955561567
1111	9	0.8005345194101849
1111	10	0.2742868416889124
1111	11	0.47021067204349265
1111	12	0.4488597806741339
1111	13	0.2673943394694476
1111	14	0.11377919270393022
1111	15	0.23874226781078534
1111	16	0.18787341038895278
1111	17	0.15423744813163154
1111	18	0.19628268823139905
1111	19	0.35208051425099995
1111	20	0.542738232708281
1111	21	0.4279593285003642
1111	22	0.3696546966455543
1111	23	0.6607598214298491
1111	24	0.575636430219653
1111	25	0.7628513212156349
1112	1	-0.13594945844167938
1112	2	-0.20889661218306088
1112	3	-0.1361369724739987
1112	4	-0.1153942912157575
1112	5	-0.07926142884380027
1112	6	-0.17512341125272002
1112	7	-0.01623599710601553
1112	8	-0.4083181496085011
1112	9	0.2388367176434727
1112	10	0.0639600091677579
1112	11	0.14843813802813344
1112	12	-0.4507070570876124
1112	13	-0.2475232419546316
1112	14	-1.040960609006973
1112	15	-0.4432146654816549
1112	16	-0.41319168870492007
1112	17	-0.5004902656799715
1112	18	-0.6262190364215172
1112	19	-0.22161868200380042
1112	20	-0.15643403399682398
1112	21	-0.43425519967801307
1112	22	-0.1365581081938103
1112	23	0.004188415167945436
1112	24	-0.059689742222111875
1112	25	-0.032354162436107003
1113	1	-2.232049994739949
1113	2	-2.24004480475104
1113	3	-2.2971850274164503
1113	4	-2.199995240008626
1113	5	-2.172397703650148
1113	6	-2.2013174969235294
1113	7	-2.50618790607594
1113	8	-2.709884372558239
1113	9	-12.39256771715348
1113	10	-5.119824856728524
1113	11	-3.4367522754505835
1113	12	-13.631068524392273
1113	13	-5.51390669617145
1113	14	-4.4117186169661515
1113	15	-3.7725889151430714
1113	16	-3.6917840683459104
1113	17	-3.807864887074061
1113	18	-4.057090429883264
1113	19	-4.7007204299012955
1113	20	-15.816448465025186
1113	21	-18.44497317349569
1113	22	-13.211964403061458
1113	23	-19.24124788268824
1113	24	-20.349090566045255
1113	25	-16.99493059355916
1114	1	-0.1695237119622064
1114	2	-0.1255013905518858
1114	3	-0.09343152997562854
1114	4	-0.4697378133172805
1114	5	-0.15356106947711368
1114	6	-0.09152512789788858
1114	7	-0.25012623628504116
1114	8	-0.14625837084287951
1114	9	0.5739676247186409
1114	10	-0.15874776968788495
1114	11	-0.22875408465603186
1114	12	-0.25547797858956045
1114	13	-0.18592099241864446
1114	14	-0.29521587365791646
1114	15	-0.15804693634985512
1114	16	-0.23298917775750283
1114	17	-0.19785641520920194
1114	18	-0.25008313445732
1114	19	-0.5229952968738161
1114	20	-0.8542430994183832
1114	21	-0.9818418225668566
1114	22	-1.1075963835890406
1114	23	-0.6483357390351898
1114	24	-0.3059521628185422
1114	25	-0.6098493416634341
1115	1	-0.18220438472803124
1115	2	-0.1309181710263232
1115	3	-0.1981871225128451
1115	4	-0.07038163677022169
1115	5	-0.3794730451518191
1115	6	-0.33404461366511384
1115	7	-0.5755898680152995
1115	8	-0.26335875070270376
1115	9	0.17713399149596398
1115	10	-0.2479255073865545
1115	11	-0.16833866752826765
1115	12	-0.1849632600772324
1115	13	-0.3254243493958384
1115	14	-0.4522334366604852
1115	15	-0.4752597747109135
1115	16	-0.23653011288660508
1115	17	-0.30505107248590946
1115	18	-0.3044509358226364
1115	19	-0.24549190141317467
1115	20	-0.7154042624821528
1115	21	-0.17523320490182215
1115	22	-0.620491519468473
1115	23	-0.3989525536754215
1115	24	-0.06121285065861315
1115	25	-0.033775453905583674
1116	1	-0.5644424503842522
1116	2	-0.5453429049812684
1116	3	-0.505895091050597
1116	4	-0.3840296705274966
1116	5	-1.334059922923302
1116	6	-1.1470086475509191
1116	7	-2.2148774675980363
1116	8	-1.4449544998409074
1116	9	-0.3563762680479172
1116	10	-1.532376594622626
1116	11	-1.1805342674875234
1116	12	-2.111255963514651
1116	13	-1.8227834688017808
1116	14	-1.287930840267876
1116	15	-2.4603694839118972
1116	16	-2.7828212539079367
1116	17	-3.3628141400355354
1116	18	-2.4917922089396676
1116	19	-2.002974301806592
1116	20	-2.416319138852007
1116	21	-1.6340249690160569
1116	22	-1.517191608137857
1116	23	-1.5772870317586154
1116	24	-2.2621961962796995
1116	25	-1.66649945253711
1201	1	0.6599850877923146
1201	2	0.7423426023260724
1201	3	0.29711733197865087
1201	4	0.7627580034386554
1201	5	0.8185433754520517
1201	6	0.9568564619053248
1201	7	1.068800475866901
1201	8	1.1054319996753748
1201	9	1.79017507932425
1201	10	1.3453514320914337
1201	11	1.3556160611223476
1201	12	1.4503060893043838
1201	13	1.2544363296396952
1201	14	1.3922291849999269
1201	15	1.4191316444752218
1201	16	1.299884345321665
1201	17	1.3204317219383555
1201	18	1.3117586605615656
1201	19	1.371259127547333
1201	20	1.6172589034245988
1201	21	1.673819297386217
1201	22	1.6129308853908313
1201	23	1.8559533826406778
1201	24	1.8978312828518682
1201	25	1.9612277977267567
1202	1	0.7615064565593954
1202	2	0.8414265021242358
1202	3	0.8209424583931053
1202	4	0.9224673222337572
1202	5	1.0079056311573993
1202	6	0.9912943813695279
1202	7	1.107134027503669
1202	8	1.1250321231172506
1202	9	1.8791192832801684
1202	10	1.4877223095986238
1202	11	1.650557096445759
1202	12	1.5479889626001366
1202	13	1.5220595165671185
1202	14	1.6099672293347638
1202	15	1.586539438676887
1202	16	1.5183729357676228
1202	17	1.515200306857686
1202	18	1.5106220246657074
1202	19	1.553890945908016
1202	20	1.7847641027313799
1202	21	1.76992562342609
1202	22	1.7331301686265448
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2211	4	0.12380561947646385
2211	5	-0.6415373910810658
2211	6	0.12405162118258062
2211	7	0.3447937102148373
2211	8	0.23416835168421812
2211	9	0.7798019906831115
2211	10	0.1477495290801727
2211	11	0.47596971109936703
2211	12	0.6147957670094684
2211	13	0.5084470701030739
2211	14	0.3788140267505469
2211	15	0.3945142347522079
2211	16	0.384043253416813
2211	17	0.3460549129183548
2211	18	0.31677022536381827
2211	19	0.011149225591651657
2211	20	0.4844112346820495
2211	21	0.7486232548313083
2211	22	0.5942232600056983
2211	23	0.942256499512439
2211	24	0.9738394096000462
2211	25	1.07329328272764
2212	1	-0.028647547014514885
2212	2	0.09722487023031334
2212	3	0.07933680710953439
2212	4	0.12635568723518276
2212	5	-0.10918844959440577
2212	6	-0.014720421192905064
2212	7	-0.012670400307882801
2212	8	-0.055083491669371654
2212	9	0.5396706325468159
2212	10	0.11491284954242786
2212	11	0.3548713078586374
2212	12	0.23762138409371403
2212	13	-0.0035199624706194754
2212	14	0.014053012439262602
2212	15	0.13026159207215215
2212	16	0.13000835738010608
2212	17	-0.05859503743911176
2212	18	-0.02606428348982348
2212	19	0.19481847739341873
2212	20	0.3630357053776123
2212	21	0.5254232326693113
2212	22	0.09351874574135621
2212	23	0.4464133398954573
2212	24	0.5183075900290177
2212	25	0.5296301153595806
2213	1	-0.23791667217113144
2213	2	-0.2276703867454053
2213	3	-0.27403901587217305
2213	4	-0.20736682352217883
2213	5	-0.22988522072219553
2213	6	-0.011617549414829398
2213	7	-0.1555451697040766
2213	8	-0.10314317018235641
2213	9	0.45829867966084237
2213	10	-0.03365296526474282
2213	11	0.1792718944435734
2213	12	0.1768293145056603
2213	13	-0.19915392771596174
2213	14	-0.09968466646772356
2213	15	-0.12469766633807211
2213	16	-0.2959039427266674
2213	17	-0.27306661156104894
2213	18	-0.46337018294614296
2213	19	-0.4515954951346046
2213	20	-0.06319398780582475
2213	21	-0.29364661739666786
2213	22	-0.07126801341274103
2213	23	-0.373667050871326
2213	24	-0.2579654895182486
2213	25	0.037029072254007644
2214	1	-0.7889599359182036
2214	2	-0.6415986015643504
2214	3	-0.668279077280804
2214	4	-0.46986112064397645
2214	5	-0.6731314368582133
2214	6	-0.48187315348602766
2214	7	-0.4953321968864799
2214	8	-0.4253252706724915
2214	9	-10.339295012414055
2214	10	-3.5044921722825677
2214	11	-1.4750156307057125
2214	12	-0.8268486329404396
2214	13	-0.6124896110776561
2214	14	-0.6281026850051745
2214	15	-0.674792444973896
2214	16	-0.80910903247905
2214	17	-0.771338240456565
2214	18	-1.0173200040953176
2214	19	-1.2165840632026956
2214	20	-0.9536923266924688
2214	21	-1.4182040322341507
2214	22	-1.2275538612442531
2214	23	-1.6816959757852565
2214	24	-1.8435795803894592
2214	25	-9.67577952729701
2215	1	-0.46036915386504873
2215	2	-0.2969981642641706
2215	3	-0.18675876240155276
2215	4	-0.35660128087057785
2215	5	-0.33107183265001083
2215	6	-0.41562617650799893
2215	7	-0.49006573339976356
2215	8	-0.5090139295954152
2215	9	0.36479508359745694
2215	10	0.14807891925109118
2215	11	0.26551032973678446
2215	12	0.24212528393061916
2215	13	0.10543330246031957
2215	14	-0.08489208598015097
2215	15	-0.4126795631501565
2215	16	-0.12170855999400487
2215	17	-0.15735298378180573
2215	18	-0.13711843979574923
2215	19	-0.19566496337530026
2215	20	-0.05521538158401995
2215	21	0.015218952061893276
2215	22	-0.061916505394052024
2215	23	0.2904660320928656
2215	24	0.41352731522086117
2215	25	0.5404551042729366
2216	1	-0.4239193910824888
2216	2	-0.4506062500304715
2216	3	-0.4501062162606786
2216	4	-0.2684402649675093
2216	5	-1.1216208884906251
2216	6	-1.5633122345534147
2216	7	-0.9360893676276056
2216	8	-1.7915205896975088
2216	9	-0.350667121060539
2216	10	-0.9638490510998565
2216	11	-0.45111190103159515
2216	12	-1.562599545426547
2216	13	-1.249833465245846
2216	14	-1.433886057947234
2216	15	-1.0553382104006204
2216	16	-2.040989610579434
2216	17	-2.415921091326716
2216	18	-2.000085023331099
2216	19	-3.3661774879242223
2216	20	-2.1741683971588586
2216	21	-2.371620371005136
2216	22	-2.1072381721690157
2216	23	-1.664488847905556
2216	24	-1.8049553771420328
2216	25	-1.8404904092333076
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	181572.0
3	4.0
4	2583.0
5	26609.0
6	35720.0
7	28509.0
8	22774.0
9	18724.0
10	16134.0
11	14337.0
12	13067.0
13	12308.0
14	12221.0
15	12686.0
16	13316.0
17	13857.0
18	15961.0
19	19475.0
20	25783.0
21	34497.0
22	42642.0
23	51581.0
24	60552.0
25	68764.0
26	77125.0
27	83950.0
28	94056.0
29	109765.0
30	134763.0
31	166080.0
32	204798.0
33	253370.0
34	313882.0
35	392601.0
36	514012.0
37	743855.0
38	1797985.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.28408381432129	19.017807362944637	13.381160291140596	24.316948531593475
2	15.950129434972915	20.263278585910044	39.27506823445155	24.511523744665492
3	19.356866475875965	24.803016179316735	28.84793417436603	26.99218317044127
4	12.201591983101077	16.468580608443638	36.28799220137172	35.041835207083565
5	13.168326718711798	38.43899433113667	33.87295093618691	14.519728013964622
6	32.263256889404296	37.11625261314881	16.90861831044563	13.711872187001264
7	29.016420557578016	32.19995888953419	20.239655894386253	18.543964658501544
8	26.51407135056076	36.086200025549225	18.70855246945331	18.691176154436707
9	26.287583427417395	15.527085725413855	19.259388771676203	38.92594207549255
10	15.025200856109464	30.686282436162713	31.582585333306092	22.70593137442173
11	35.077760192304176	23.31394903576935	21.690938351750106	19.917352420176375
12	23.13491455862484	26.93390181746998	29.2925413635448	20.63864226036038
13	28.791539529763092	21.767116810349574	25.78299102732302	23.658352632564313
14	21.803760805948908	21.490662288805936	27.82770095876429	28.877875946480863
15	23.998622967802813	29.293429005570303	23.615373875254953	23.092574151371934
16	22.899096818935323	29.094530961893874	25.38735674408591	22.619015475084893
17	21.96273361657438	28.459827515120274	26.824100701979958	22.753338166325392
18	21.531353609047947	27.33206573846698	29.106686708001178	22.02989394448389
19	22.889646728258683	26.58308917530132	28.883729375071503	21.64353472136849
20	22.661230508218637	26.245790752312807	29.336211299570525	21.756767439898027
21	23.546432307062624	25.654553128837144	28.793249607758426	22.005764956341807
22	22.962822295182615	25.952465291014725	28.981651855296832	22.10306055850583
23	22.41690730908719	25.891271075160017	29.176484214236176	22.515337401516618
24	22.310368722574246	26.305436150206134	29.15228784281138	22.231907284408237
25	22.397190206763838	25.7192386800359	29.205216653131828	22.678354460068434
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1628.0
1	1628.0
2	2539.5
3	3451.0
4	3451.0
5	3451.0
6	8829.5
7	14208.0
8	14208.0
9	14208.0
10	18175.5
11	22143.0
12	22143.0
13	22143.0
14	27478.5
15	32814.0
16	32814.0
17	32814.0
18	53399.0
19	73984.0
20	73984.0
21	73984.0
22	111123.5
23	148263.0
24	148263.0
25	148263.0
26	208473.0
27	268683.0
28	268683.0
29	268683.0
30	321144.5
31	373606.0
32	373606.0
33	373606.0
34	418579.0
35	463552.0
36	463552.0
37	463552.0
38	504124.0
39	544696.0
40	544696.0
41	544696.0
42	587354.0
43	630012.0
44	630012.0
45	630012.0
46	670186.0
47	710360.0
48	710360.0
49	710360.0
50	705732.0
51	701104.0
52	701104.0
53	701104.0
54	646894.5
55	592685.0
56	592685.0
57	592685.0
58	518339.0
59	443993.0
60	443993.0
61	443993.0
62	370296.0
63	296599.0
64	296599.0
65	296599.0
66	230438.0
67	164277.0
68	164277.0
69	164277.0
70	122588.0
71	80899.0
72	80899.0
73	80899.0
74	57397.5
75	33896.0
76	33896.0
77	33896.0
78	25830.5
79	17765.0
80	17765.0
81	17765.0
82	12340.0
83	6915.0
84	6915.0
85	6915.0
86	4934.0
87	2953.0
88	2953.0
89	2953.0
90	2148.0
91	1343.0
92	1343.0
93	1343.0
94	713.0
95	83.0
96	83.0
97	83.0
98	44.5
99	6.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02792225392980857
2	0.001136783875004929
3	2.486714726573282E-4
4	0.003730072089859923
5	0.035204775629058896
6	0.0123270001445847
7	0.020977214943450333
8	0.027833442689573808
9	0.035027153148589375
10	0.037886875084148654
11	0.03849079151774502
12	0.007886438132846695
13	0.0028419596875123225
14	0.006749654257841767
15	0.007460144179719846
16	0.008561403558630871
17	0.00985804766605837
18	0.015719589521552536
19	0.018650360449299616
20	0.016749799908275752
21	0.01303749006646278
22	0.012149377664115179
23	0.009627138441447993
24	0.007122661466827758
25	0.10939768572117746
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	5629918.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.82201086779362
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.60427056427511	27.021777482911357
2	13.52828413576459	10.503903874825058
3	5.918020738969427	6.892483963322975
4	3.1460150338880872	4.885385193433819
5	1.860377779958478	3.6111803195874903
6	1.1995404801321152	2.7941144133628413
7	0.8511387762725984	2.313004317571877
8	0.6047043723379376	1.8780671771764588
9	0.4674041305911229	1.6331011413714185
>10	2.490727000312384	17.523977959382904
>50	0.19379933094277002	5.196385904620351
>100	0.1249429421596849	9.11863280954325
>500	0.007965924747754323	2.137493120921519
>1k	0.002394378060427593	1.5006800466205736
>5k	2.3022865965362395E-4	0.68060621911346
>10k+	1.8418292772289917E-4	2.309206056234616
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	47408	0.8420726554099012	No Hit
TATCAACGCAGAGTACTTTTTTTTT	33891	0.6019803485592508	No Hit
GGTATCAACGCAGAGTACTTTTTTT	31879	0.5662427054887833	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	16002	0.28423149324732616	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	9573	0.17003800055347165	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	9110	0.16181407970773287	No Hit
GTGGTATCAACGCAGAGTACATGGG	8386	0.14895421212173962	No Hit
GTGGTATCAACGCAGAGTACTTTTT	5644	0.10025012797699717	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	1.7762248046952018E-5
6	0.0	0.0	0.0	0.0	1.7762248046952018E-5
7	0.0	0.0	0.0	0.0	1.7762248046952018E-5
8	0.0	0.0	0.0	0.0	1.7762248046952018E-5
9	0.0	0.0	0.0	0.0	1.7762248046952018E-5
10	0.0	0.0	0.0	0.0	1.7762248046952018E-5
11	0.0	0.0	0.0	1.7762248046952018E-5	1.7762248046952018E-5
12	1.7762248046952018E-5	0.0	0.0	3.5524496093904035E-5	1.7762248046952018E-5
13	3.5524496093904035E-5	0.0	0.0	3.5524496093904035E-5	1.7762248046952018E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAACG	395	0.0	14.190577	2
GGACCGA	520	0.0	14.068379	6
GGTATCA	8515	0.0	13.524013	1
ACGACCG	50	0.0014970297	13.303969	5
GTGGTAT	2760	0.0	12.737383	1
TGGTATC	2580	0.0	12.6672945	2
AGGACCG	655	0.0	12.477006	5
CGAGTGG	515	0.0	12.359286	10
TAACGCA	470	0.0	12.331604	4
ATAACGC	470	0.0	12.330727	3
ACCGAGT	560	0.0	12.045133	8
GCGTATC	65	8.019243E-4	11.69403	1
TATCACG	190	0.0	11.50057	2
TTGCGCG	470	0.0	11.114411	18
GCGCGGT	105	3.4769528E-6	10.855558	11
ATTTGCG	505	0.0	10.720637	16
CGCGCCA	170	1.4006218E-10	10.6177025	10
ATCGGAC	90	9.516969E-5	10.55636	3
CGTATCA	90	9.519541E-5	10.556079	2
GACCGAG	675	0.0	10.2748575	7
>>END_MODULE