FastQCFastQC Report
Thu 26 May 2016
SRR1364284_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364284_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1174058
Sequences flagged as poor quality0
Sequence length25
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA101550.8649487504024503No Hit
GTATCAACGCAGAGTACTTTTTTTT97580.8311344073291099No Hit
GTACTTTTTTTTTTTTTTTTTTTTT64800.5519318466379004No Hit
TATCAACGCAGAGTACTTTTTTTTT60440.5147956915246096No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA46370.3949549340833247No Hit
GGTATCAACGCAGAGTACTTTTTTT40460.34461670547792356No Hit
GAACAAAAAAAAAAAAAAAAAAAAA38330.3264745012597333No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN32680.2783508140142991No Hit
GAGTACTTTTTTTTTTTTTTTTTTT28890.24606961495939722No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20970.1786112781480983No Hit
AAACAAAAAAAAAAAAAAAAAAAAA19970.1700938113789949No Hit
GTGGTATCAACGCAGAGTACATGGG14360.12231082280432483No Hit
GATATAGGCTTACTAGGAGGGTGAA13070.11132329067218144No Hit
GAAAACAAAAAAAAAAAAAAAAAAA12860.10953462265066974No Hit
GTATCAACGCAGATTACTTTTTTTT12150.10348722124460631No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG402.7533737E-416.630545
ACGTCAT350.00218544416.26817311
CGACGGG651.893768E-716.09054415
ATAACGC1850.014.8828453
CTCAACG456.7917776E-414.7688013
TATAACG1800.014.7688012
GCCACGT653.373705E-614.6140038
TATCACG1300.014.6065062
TAGGGTC400.005266410314.254754
CTGGTCG1500.013.931429
CGCATAT1101.8553692E-1013.8168768
TAACGCA2150.013.7022414
TTAGACT707.2339135E-613.5759534
GTTATCA3450.013.4839811
CCCGCCC500.001512358813.28453813
ATCACGC1600.013.0545653
GTTCTAG802.012839E-613.0540071
GAACAAA11150.012.8571061
TAAGACT751.4727853E-512.6708894
ACCGAGT604.0939433E-412.6654688
Produced by FastQC (version 0.11.5)