##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364284_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1174058
Sequences flagged as poor quality	0
Sequence length	25
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.833899177042362	30.0	26.0	31.0	7.0	34.0
2	25.7448337305312	30.0	25.0	31.0	7.0	34.0
3	25.334404262821767	30.0	23.0	31.0	7.0	34.0
4	28.852359934517715	35.0	27.0	37.0	7.0	37.0
5	29.670164506353178	35.0	30.0	37.0	2.0	37.0
6	29.6213858259132	35.0	30.0	37.0	2.0	37.0
7	29.23198428016333	35.0	29.0	37.0	2.0	37.0
8	28.651184183404908	35.0	27.0	37.0	2.0	37.0
9	29.582492517405445	35.0	27.0	39.0	2.0	39.0
10	29.101303342765007	35.0	26.0	39.0	2.0	39.0
11	29.625343040974126	35.0	27.0	39.0	2.0	39.0
12	29.23539722909771	35.0	27.0	39.0	2.0	39.0
13	28.807487364338048	35.0	25.0	39.0	2.0	39.0
14	29.11590483604728	36.0	23.0	40.0	2.0	41.0
15	29.81861884165859	36.0	27.0	40.0	2.0	41.0
16	30.15212451173622	36.0	27.0	40.0	2.0	41.0
17	30.33932139638757	37.0	27.0	40.0	2.0	41.0
18	30.18570717971344	37.0	27.0	40.0	2.0	41.0
19	30.168607513427787	37.0	27.0	40.0	2.0	41.0
20	30.060379470179498	37.0	27.0	40.0	2.0	41.0
21	29.95518620034104	37.0	27.0	40.0	2.0	41.0
22	30.040211812363616	37.0	27.0	40.0	2.0	41.0
23	30.048528266916968	37.0	27.0	40.0	2.0	41.0
24	29.94016394420037	37.0	27.0	40.0	2.0	41.0
25	29.7722753049679	37.0	27.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.5995900341852334
1101	2	0.7092388475221512
1101	3	1.4418517523373566
1101	4	0.9149057459567302
1101	5	0.3801283917873164
1101	6	0.23384570248241587
1101	7	0.49814692369490743
1101	8	0.7468930027440734
1101	9	0.7705803860657312
1101	10	1.1479419291422417
1101	11	0.7445820576750712
1101	12	1.0251303692234188
1101	13	0.9183562591666536
1101	14	1.4145363578120502
1101	15	0.8995175506310389
1101	16	0.356498029601962
1101	17	-0.03250386487278689
1101	18	-5.43569419752891
1101	19	-6.794568684854614
1101	20	-8.257765151707403
1101	21	-9.716961031411433
1101	22	-9.95703469415227
1101	23	-11.28073135035434
1101	24	-10.842850157337956
1101	25	-11.220212596807805
1102	1	2.0962260048215455
1102	2	2.2050522760485833
1102	3	2.975294880036177
1102	4	2.4815994135728054
1102	5	1.795465460082422
1102	6	1.866521897411772
1102	7	2.1658325862518133
1102	8	2.2787981750841944
1102	9	2.6861219453491927
1102	10	-0.6519209092903466
1102	11	1.4078610920381038
1102	12	2.1686918941523956
1102	13	2.7086022221482935
1102	14	3.312427292696018
1102	15	2.937227520146621
1102	16	2.6966007268075813
1102	17	2.442567157594368
1102	18	2.564277723111495
1102	19	2.2421459934474868
1102	20	2.6714272492853866
1102	21	2.74169363763669
1102	22	2.6746370532506916
1102	23	2.7351359295004265
1102	24	2.913704847075387
1102	25	2.997308740453388
1103	1	-0.37614834242952355
1103	2	0.03139583672867019
1103	3	-2.399922248459351
1103	4	-0.11039444183847635
1103	5	-2.4795430614440903
1103	6	-6.065687654878584
1103	7	-6.739772533026834
1103	8	-1.7507576602299189
1103	9	-5.974554959268794
1103	10	-3.8515146029062457
1103	11	-7.242240030076509
1103	12	-1.8914705375049614
1103	13	-0.12213162899086427
1103	14	0.3277569507896487
1103	15	0.024450684155329583
1103	16	0.330102369757892
1103	17	-0.040061199318980556
1103	18	-0.02139520479412127
1103	19	-5.018516367765251
1103	20	-1.3103971300066242
1103	21	-5.687096850976282
1103	22	-2.3774028865759647
1103	23	-1.7929114820006795
1103	24	-5.919707717517785
1103	25	-3.1921391100767664
1104	1	1.208415843266252
1104	2	1.227383355863136
1104	3	2.1100439303818774
1104	4	1.3858554283406512
1104	5	1.210587391596878
1104	6	1.0092201158600176
1104	7	1.2131027170194528
1104	8	1.4752742463532407
1104	9	1.650755369053833
1104	10	1.8122389587010943
1104	11	1.3583343426064722
1104	12	1.4923811009473056
1104	13	1.7430118061370834
1104	14	2.1382907290510857
1104	15	1.1807766858080058
1104	16	0.6580998771971913
1104	17	0.5221774091819178
1104	18	0.646668861240677
1104	19	1.0461104931886958
1104	20	-9.416339087660187
1104	21	-3.7386874388503344
1104	22	-1.118395124416832
1104	23	-0.16526392647121213
1104	24	0.5148575970551157
1104	25	1.0806138599989588
1105	1	1.2865304695532878
1105	2	1.147419956300162
1105	3	2.001489003064158
1105	4	1.463728337044067
1105	5	1.1934058127620801
1105	6	1.1444926004296434
1105	7	1.4951880160783055
1105	8	1.5394020677834241
1105	9	1.4708937545678893
1105	10	1.9933888546599867
1105	11	1.6035871557596586
1105	12	1.870855461596971
1105	13	1.9663474267535683
1105	14	2.44851517774455
1105	15	1.9568260770605832
1105	16	1.4775781539647035
1105	17	0.9040211664517557
1105	18	1.4689056483715746
1105	19	1.4777117417991867
1105	20	1.4230174572023024
1105	21	1.4963209436224751
1105	22	1.4641905446896715
1105	23	1.411283783891939
1105	24	1.3033277562136938
1105	25	1.548678132850938
1106	1	1.0857448747271903
1106	2	1.360023773803718
1106	3	2.052489388646684
1106	4	1.659875705265918
1106	5	0.9094970231363817
1106	6	0.5911781283110464
1106	7	1.1342053010569089
1106	8	1.0761495017672011
1106	9	1.0281811460193104
1106	10	-1.8221128639026176
1106	11	0.009083362621133517
1106	12	0.5710360703442525
1106	13	1.4325184740004389
1106	14	0.7216075495975005
1106	15	1.1197914668892466
1106	16	0.7293759620581497
1106	17	-5.747724481551611
1106	18	-1.690587133192377
1106	19	-0.23995314204112006
1106	20	0.2958698453301487
1106	21	0.6681192484786855
1106	22	0.8490841073539279
1106	23	0.17386607500910856
1106	24	0.5247981976465468
1106	25	0.5579037270146863
1107	1	0.6097012478104524
1107	2	1.0043910445048319
1107	3	1.453402215413437
1107	4	0.9577510716173094
1107	5	0.6734198361543697
1107	6	0.699249014457969
1107	7	0.6894260931118303
1107	8	0.7796568965459407
1107	9	1.052728587429371
1107	10	-1.549856104288704
1107	11	-0.09264843253280475
1107	12	0.3506648303740185
1107	13	1.1498286575470118
1107	14	1.603732930842412
1107	15	1.1090979194630215
1107	16	-4.808568930749388
1107	17	-1.683207504690884
1107	18	-0.17640441364729043
1107	19	0.7094509419373054
1107	20	1.0416181833885219
1107	21	1.0910064420372088
1107	22	0.6359248354659996
1107	23	0.4419590736529244
1107	24	0.6529779641862383
1107	25	0.8589245464035962
1108	1	-0.7161833792455496
1108	2	-3.2071765955015508
1108	3	-0.31901275110992344
1108	4	-0.9209857793506799
1108	5	-1.0372251570836468
1108	6	-0.738161856285533
1108	7	-0.17219618652731938
1108	8	-0.1473461327524781
1108	9	-0.6124339545150974
1108	10	-3.0254103501302154
1108	11	-1.3296441122305431
1108	12	-5.567631816348744
1108	13	-1.5329492459399248
1108	14	-5.628104872767139
1108	15	-2.669120425070119
1108	16	-7.347257825134399
1108	17	-3.381133654359626
1108	18	-2.004864032064443
1108	19	-0.7845571163122251
1108	20	-0.6902117268551358
1108	21	-0.25631919789550395
1108	22	-0.22484583769778155
1108	23	-0.27158190782573044
1108	24	0.025756200948624297
1108	25	-0.24686497436385224
1109	1	-8.501142264248646
1109	2	-5.792623365902408
1109	3	-5.082572246912179
1109	4	-4.8630146903587175
1109	5	-2.4694749416133064
1109	6	-1.6008991092657077
1109	7	-1.153191388206988
1109	8	-0.6890528520573085
1109	9	-5.366941982463128
1109	10	-1.599594118773613
1109	11	-1.1704662179740772
1109	12	-6.160606198081659
1109	13	-7.666556279440208
1109	14	-7.548853767142976
1109	15	-3.463055670306499
1109	16	-2.1498749973067675
1109	17	-1.8102803232652427
1109	18	-1.3073181378810865
1109	19	-1.0818390771751574
1109	20	-0.8693988053742991
1109	21	-0.7005050969679978
1109	22	-0.9104633280066139
1109	23	-1.1416611449379204
1109	24	-0.8401635589811463
1109	25	-0.7085652281305137
1110	1	-2.744073079464936
1110	2	-2.551490335117638
1110	3	-1.7637608012580372
1110	4	-2.5876027027099653
1110	5	-5.3165629878980845
1110	6	-4.17279400452745
1110	7	-8.331688667569118
1110	8	-8.813965629041455
1110	9	-10.529029007972003
1110	10	-8.922410828695138
1110	11	-11.224334099931937
1110	12	-6.223739179558937
1110	13	-4.88329650568404
1110	14	-4.377032389907136
1110	15	-9.47587367939736
1110	16	-10.973949740817776
1110	17	-11.723321296406787
1110	18	-8.518551160383307
1110	19	-11.570961927347692
1110	20	-12.548015336847023
1110	21	-13.200006343367235
1110	22	-13.706512855998174
1110	23	-14.271806610352177
1110	24	-14.815922001019494
1110	25	-15.345698922627381
1111	1	-0.8224685944859225
1111	2	-0.11649532396617346
1111	3	-2.9021837347566724
1111	4	-3.62714117225989
1111	5	-1.1453612448782238
1111	6	-0.7485152015503473
1111	7	-0.3207385418523607
1111	8	-0.06612679043091418
1111	9	-4.677368374428617
1111	10	-4.198295684455733
1111	11	-1.819580728386505
1111	12	-5.819753558804436
1111	13	-1.5459578913837113
1111	14	-0.5221779464902596
1111	15	-0.4391037910456461
1111	16	-0.4103666364590879
1111	17	-0.5894046172784435
1111	18	-0.10882926884808697
1111	19	-0.06450510699441736
1111	20	-0.030405662240248432
1111	21	0.11188816873228902
1111	22	-0.31209752593355944
1111	23	-0.30703988322896336
1111	24	-0.21870740200289518
1111	25	-0.04256016691833153
1112	1	-0.42139686254954256
1112	2	0.14584653610226894
1112	3	-2.231472473727454
1112	4	-0.42926438463760164
1112	5	-0.4970233221567888
1112	6	-0.3860488090489902
1112	7	0.12231625070672436
1112	8	-2.9527637701172402
1112	9	-0.6184935703119159
1112	10	-2.6946210364181624
1112	11	-0.7052208210984183
1112	12	0.5042244395149744
1112	13	0.9422043966815394
1112	14	1.6821530781726395
1112	15	0.9196180714986149
1112	16	0.6990616039370003
1112	17	0.4074526929693292
1112	18	0.5345963827794193
1112	19	0.6191636880758544
1112	20	0.628022639195553
1112	21	0.44108477766222975
1112	22	0.48548906632169775
1112	23	0.29122094119513875
1112	24	0.6057997022786132
1112	25	0.47102117021658074
1113	1	-1.4981845174943444
1113	2	-1.1629342373083809
1113	3	-3.719690453138991
1113	4	-1.902012947976992
1113	5	-2.3805645852952644
1113	6	-1.7688521920689269
1113	7	-1.337310879220329
1113	8	-8.614110882559743
1113	9	-8.916984124243331
1113	10	-3.4252767471691534
1113	11	-8.915105607534258
1113	12	-7.165654411798172
1113	13	-3.035280234157838
1113	14	-1.299199407962032
1113	15	-7.047002878682445
1113	16	-3.1202740633432313
1113	17	-2.37230011719096
1113	18	-1.9330130861474046
1113	19	-1.3976110539668696
1113	20	-6.348280982432353
1113	21	-2.561618109293736
1113	22	-2.016826834593836
1113	23	-1.841775934845014
1113	24	-7.5080804643860475
1113	25	-4.331906689971181
1114	1	-4.204945327672064
1114	2	-3.8188125281230008
1114	3	-0.932337767858602
1114	4	-0.7684972901791234
1114	5	-0.9867072364430598
1114	6	-0.8578362655503504
1114	7	-0.6735707331579484
1114	8	-0.2172134405780639
1114	9	-0.5656931494057318
1114	10	-3.0834913870732663
1114	11	-1.2048544702879447
1114	12	-4.49734601371836
1114	13	-1.0501075329367318
1114	14	-6.047043051044145
1114	15	-2.22061572211258
1114	16	-1.397125346205744
1114	17	-1.0374507934545072
1114	18	-0.7129217008262501
1114	19	-0.6430534493736708
1114	20	-0.2772302134855167
1114	21	-0.16902526882459767
1114	22	-0.32384784753907425
1114	23	-0.6566486626339803
1114	24	-0.6022036946320348
1114	25	-0.4855287408881743
1115	1	0.14970630520176087
1115	2	0.2666445071796879
1115	3	1.0075721689644048
1115	4	-0.14438807049118552
1115	5	-0.16379517102427243
1115	6	-0.7510226313028525
1115	7	-2.4809970727647226
1115	8	-0.5963315840272081
1115	9	-0.5927163579936483
1115	10	0.20400351697821506
1115	11	-0.7408010145326926
1115	12	0.042610052609401095
1115	13	0.318616152180887
1115	14	0.8223491714532649
1115	15	0.13588109095143253
1115	16	-0.32039121301063034
1115	17	-0.905177417413082
1115	18	-0.3585679522082188
1115	19	-0.23041566726664087
1115	20	-0.1483271850239518
1115	21	-0.16952145888496872
1115	22	-0.44209172623039095
1115	23	-0.3347923841275211
1115	24	-0.5387500926581694
1115	25	-0.2966641650285453
1116	1	-0.42721599018207357
1116	2	-0.3263117151968906
1116	3	0.42417694542843876
1116	4	-0.315788872588211
1116	5	-1.2273441741841786
1116	6	-1.9059035431050937
1116	7	-5.215153967934313
1116	8	-1.9668697297137392
1116	9	-2.003010766467842
1116	10	-1.3784687197208285
1116	11	-3.152521544993977
1116	12	-1.6264071538686231
1116	13	-7.36483198926706
1116	14	-2.5950125973157796
1116	15	-2.3146455267010566
1116	16	-2.0665251860525444
1116	17	-1.6899291597117205
1116	18	-1.352735533991293
1116	19	-1.2213329043964123
1116	20	-1.1534369546321273
1116	21	-1.1255025709968152
1116	22	-1.1704073310615186
1116	23	-1.2193397528136813
1116	24	-1.1877374830838896
1116	25	-1.1898581316424668
1201	1	2.0813954438809326
1201	2	2.1243734008464585
1201	3	2.8263671140917737
1201	4	2.2848010586341836
1201	5	1.8499523093441361
1201	6	1.7886572487131502
1201	7	2.0300884243578174
1201	8	2.1425932810510595
1201	9	2.4578091452198088
1201	10	2.6017006685047512
1201	11	2.318806186035715
1201	12	2.464770229010803
1201	13	2.741629409624693
1201	14	3.229021017750515
1201	15	2.667225646033806
1201	16	2.2901567137114114
1201	17	2.1054475864451874
1201	18	1.9393298879563758
1201	19	2.0409855109964283
1201	20	2.2234647584457576
1201	21	2.188986584093623
1201	22	1.3625318436756615
1201	23	1.1892310188662591
1201	24	1.7139864837353578
1201	25	1.362935921631788
1202	1	2.061666783620897
1202	2	2.3553682517816483
1202	3	2.9510688001339993
1202	4	2.428955211066164
1202	5	1.964538049261062
1202	6	1.9513474200209409
1202	7	2.2305188443937
1202	8	2.3775854161310583
1202	9	2.475449607775417
1202	10	-0.8182802221974406
1202	11	1.2564355271028838
1202	12	2.226551018943706
1202	13	2.6435059303807087
1202	14	3.3197920256891713
1202	15	2.5864483092923436
1202	16	2.517246140309588
1202	17	2.04875410941321
1202	18	2.280719336674643
1202	19	2.4723723790378216
1202	20	2.4818891323243975
1202	21	2.53245009823819
1202	22	2.1488895869351765
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2211	19	1.362399594353473
2211	20	1.5483538462599604
2211	21	1.5060959568731143
2211	22	1.393616826540363
2211	23	1.3199803583311898
2211	24	1.5584243377421032
2211	25	1.4895932883168186
2212	1	0.8564785842312759
2212	2	0.8883899466819081
2212	3	1.4562144279813154
2212	4	0.9425604125346041
2212	5	0.5700248023240029
2212	6	0.3476884788912997
2212	7	0.7424880823078048
2212	8	1.1297692809636501
2212	9	0.78494306170969
2212	10	1.5934070483585323
2212	11	0.94148241108714
2212	12	1.310100711543999
2212	13	1.3058063417213255
2212	14	-4.190708217576784
2212	15	-0.6651942887880686
2212	16	-0.09222851580080871
2212	17	0.22232713343019483
2212	18	0.4456188236046259
2212	19	0.8540406306190462
2212	20	1.033457644863649
2212	21	0.9858978402878122
2212	22	0.9507710279542394
2212	23	0.922811266915776
2212	24	0.8550916020065671
2212	25	0.8663578594129611
2213	1	0.7208911022791789
2213	2	0.7845827718800891
2213	3	1.4559601044818251
2213	4	0.9440996828554269
2213	5	0.32175708125449987
2213	6	0.1895093924938216
2213	7	0.3373128923382822
2213	8	-5.029863768081864
2213	9	-1.5046194378904296
2213	10	0.3027126225751431
2213	11	-0.047021313659122654
2213	12	0.3732582470906394
2213	13	-7.638482949393239
2213	14	-2.23203551965765
2213	15	-0.8075551999593245
2213	16	-0.32298150530156633
2213	17	-0.2824884846985398
2213	18	0.02887697955131685
2213	19	0.4262610400532232
2213	20	0.6525743478927097
2213	21	0.6702679817492623
2213	22	0.4044964140247771
2213	23	0.2841836327747913
2213	24	0.38828428431439477
2213	25	0.4207372354299892
2214	1	-1.1020122158579966
2214	2	-0.6994522502914435
2214	3	-7.067359332725054
2214	4	-2.791136775249175
2214	5	-1.689599069256282
2214	6	-1.0879988200950628
2214	7	-0.7859704389739939
2214	8	-5.825642601178682
2214	9	-1.7784952117637438
2214	10	-0.2888800024271774
2214	11	-0.5395876507297714
2214	12	-0.20124935262663257
2214	13	-5.710690095629214
2214	14	-1.3542728178661214
2214	15	-0.7319078884555807
2214	16	-0.7255594989499876
2214	17	-0.6660472477247943
2214	18	-0.6626159035999599
2214	19	-0.3337418221992401
2214	20	-0.02925542892807087
2214	21	0.07853388281801799
2214	22	-0.29906026239758177
2214	23	-0.36296591336154904
2214	24	-0.26398454236966273
2214	25	-0.22120099017012862
2215	1	0.2019717945141437
2215	2	0.41550725907538677
2215	3	-2.4013383704637086
2215	4	-0.7005749584895824
2215	5	-0.31351442776839633
2215	6	-0.6591361453859008
2215	7	-0.2010703855011542
2215	8	0.3840384804485737
2215	9	0.4905710911513772
2215	10	0.9181399255718148
2215	11	0.22490905177845733
2215	12	0.039606337689562565
2215	13	0.5749116327582797
2215	14	0.6912801378422451
2215	15	0.34925616190198383
2215	16	0.09667566574294995
2215	17	0.19783173563277856
2215	18	0.09993999595284464
2215	19	0.185623065785947
2215	20	0.5532505512881158
2215	21	0.46318326106853647
2215	22	0.4173216699132958
2215	23	0.3644648018461929
2215	24	0.32221571852329944
2215	25	0.34083630231041084
2216	1	0.03587730993668714
2216	2	-0.16106767499180918
2216	3	0.6901706307976134
2216	4	-0.4388022260047748
2216	5	-0.6546381521119358
2216	6	-0.7766960582399314
2216	7	-1.2400246338671685
2216	8	-5.717529000344914
2216	9	-1.8468902091582216
2216	10	-1.046180679863344
2216	11	-1.299228088457756
2216	12	-0.11899818014096297
2216	13	0.22565696012899394
2216	14	0.7999064421651454
2216	15	0.23132817199256905
2216	16	-0.2698534020245056
2216	17	-0.4641921148430832
2216	18	-0.09419485096837832
2216	19	-7.454699324327407E-4
2216	20	0.11857677534499089
2216	21	-0.1382821672080361
2216	22	-0.4172259737055555
2216	23	-0.6785417341015183
2216	24	-0.58120705409452
2216	25	-0.5053854612488173
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	116770.0
3	2.0
4	616.0
5	5388.0
6	10067.0
7	8706.0
8	7311.0
9	5761.0
10	5221.0
11	4869.0
12	4962.0
13	4714.0
14	4610.0
15	4363.0
16	4166.0
17	4164.0
18	5054.0
19	6784.0
20	9537.0
21	12289.0
22	14802.0
23	16382.0
24	18807.0
25	20097.0
26	21640.0
27	22768.0
28	24351.0
29	27769.0
30	31823.0
31	35603.0
32	44540.0
33	56098.0
34	71676.0
35	91622.0
36	117833.0
37	142519.0
38	190374.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.110358643370176	19.467197002587866	13.158974288849612	20.263470065192347
2	13.629817656585857	25.01416612181583	41.169613134537556	20.186403087060754
3	14.462005299598257	34.53406274040516	30.00239336695444	21.00153859304214
4	9.346006889536623	20.4589320363453	38.31052492114643	31.88453615297165
5	10.362537506090733	43.510399124644174	32.399534967216894	13.727528402048195
6	24.83955793855018	41.935778750568126	19.551121044052366	13.67354226682933
7	25.06350355047432	37.417069446782186	22.09641603305517	15.423010969688322
8	24.73558795208928	36.619285098515334	21.164327207079126	17.480799742316265
9	27.219553205795833	20.071221870308868	19.929820484318974	32.779404439576325
10	12.456294005350777	35.13380363606506	31.73961832949017	20.670284029093995
11	29.42047942940775	30.040506376699906	23.16407688436673	17.37493730952562
12	21.21351927938642	33.51087234193432	28.526879047064924	16.74872933161433
13	25.11988271869145	28.01389810844591	27.0648319249837	19.80138724787893
14	19.663021664113543	27.302233906127586	29.76835622887051	23.26638820088837
15	21.443710793103772	34.32505832713256	24.475183951516932	19.756046928246736
16	19.874598883880516	34.103071891627735	25.747810307530898	20.274518916960847
17	18.874853917072738	33.78650364609318	26.512940726211546	20.825701710622536
18	18.334785654484257	31.90510362738565	30.37000017086131	19.390110547268783
19	20.14800095341182	31.6143158714876	30.254764282596046	17.982918892504525
20	20.818703639348584	30.598158327669765	30.102988480560413	18.480149552421242
21	22.2913644541722	30.001093518001188	29.221876511062472	18.485665516764143
22	21.064736105581687	30.5066355917868	29.2364157039776	19.192212598653917
23	20.526874674835955	30.894022790742575	29.426694675980702	19.152407858440768
24	19.87583317528419	30.80565764675931	29.886495085098787	19.432014092857717
25	19.88370136177214	30.527922693098535	30.384919960328272	19.20345598480105
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	14576.0
1	14576.0
2	14362.5
3	14149.0
4	14149.0
5	14149.0
6	18872.0
7	23595.0
8	23595.0
9	23595.0
10	21346.5
11	19098.0
12	19098.0
13	19098.0
14	18746.5
15	18395.0
16	18395.0
17	18395.0
18	25065.0
19	31735.0
20	31735.0
21	31735.0
22	40541.0
23	49347.0
24	49347.0
25	49347.0
26	64695.5
27	80044.0
28	80044.0
29	80044.0
30	92249.5
31	104455.0
32	104455.0
33	104455.0
34	108636.0
35	112817.0
36	112817.0
37	112817.0
38	116515.0
39	120213.0
40	120213.0
41	120213.0
42	124477.0
43	128741.0
44	128741.0
45	128741.0
46	129736.0
47	130731.0
48	130731.0
49	130731.0
50	120864.0
51	110997.0
52	110997.0
53	110997.0
54	96726.5
55	82456.0
56	82456.0
57	82456.0
58	69951.5
59	57447.0
60	57447.0
61	57447.0
62	46613.0
63	35779.0
64	35779.0
65	35779.0
66	27681.0
67	19583.0
68	19583.0
69	19583.0
70	14849.0
71	10115.0
72	10115.0
73	10115.0
74	7450.5
75	4786.0
76	4786.0
77	4786.0
78	3834.5
79	2883.0
80	2883.0
81	2883.0
82	2001.5
83	1120.0
84	1120.0
85	1120.0
86	861.0
87	602.0
88	602.0
89	602.0
90	469.5
91	337.0
92	337.0
93	337.0
94	192.5
95	48.0
96	48.0
97	48.0
98	28.5
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3391655267456974
2	0.34180594144411947
3	0.3541562682593194
4	0.35500801493622974
5	0.36182198835151247
6	0.3016886729616424
7	0.3109727117399651
8	0.30926921838614446
9	0.30926921838614446
10	0.41582272766762807
11	0.30875817037999825
12	0.30756572503232377
13	0.3014331489585693
14	0.30637327968464934
15	0.2985372102570742
16	0.30441426232775554
17	0.29725959024170867
18	0.29964448093705764
19	0.3002407036108949
20	0.49162818191264823
21	0.3001555289432038
22	0.30254041963855277
23	0.29768546358016384
24	0.30117762495549627
25	0.29393777820175837
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1174058.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.953741449550854
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.16543872783737	48.21278680464528
2	7.329572839077782	8.202340471468172
3	2.378363920098705	3.992350795744295
4	1.1056856425974853	2.4746899428152074
5	0.6506884217862726	1.8204225858422696
6	0.4210296648624317	1.4134911006182131
7	0.2823238008958396	1.1057951072266197
8	0.20483452726268617	0.9169006542717854
9	0.16461252108822255	0.828961779989623
>10	1.0928903563975954	12.081144123020746
>50	0.12691347310826653	4.900731454265174
>100	0.07199077967746786	7.587808842340457
>500	0.003209779229899589	1.202288071939656
>1k	0.0018341595599426224	2.486165475265742
>5k	4.585398899856556E-4	1.905632580662926
>10k+	1.5284662999521853E-4	0.8684902098838531
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAA	10155	0.8649487504024503	No Hit
GTATCAACGCAGAGTACTTTTTTTT	9758	0.8311344073291099	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	6480	0.5519318466379004	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6044	0.5147956915246096	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	4637	0.3949549340833247	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4046	0.34461670547792356	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	3833	0.3264745012597333	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	3268	0.2783508140142991	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2889	0.24606961495939722	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2097	0.1786112781480983	No Hit
AAACAAAAAAAAAAAAAAAAAAAAA	1997	0.1700938113789949	No Hit
GTGGTATCAACGCAGAGTACATGGG	1436	0.12231082280432483	No Hit
GATATAGGCTTACTAGGAGGGTGAA	1307	0.11132329067218144	No Hit
GAAAACAAAAAAAAAAAAAAAAAAA	1286	0.10953462265066974	No Hit
GTATCAACGCAGATTACTTTTTTTT	1215	0.10348722124460631	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	8.517466769103401E-5	0.0	0.0	0.0	0.0
6	8.517466769103401E-5	0.0	0.0	0.0	0.0
7	8.517466769103401E-5	0.0	0.0	0.0	0.0
8	8.517466769103401E-5	0.0	0.0	0.0	0.0
9	8.517466769103401E-5	0.0	0.0	0.0	0.0
10	8.517466769103401E-5	0.0	0.0	0.0	0.0
11	8.517466769103401E-5	0.0	0.0	0.0	0.0
12	8.517466769103401E-5	0.0	0.0	0.0	0.0
13	8.517466769103401E-5	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGACCG	40	2.7533737E-4	16.63054	5
ACGTCAT	35	0.002185444	16.268173	11
CGACGGG	65	1.893768E-7	16.090544	15
ATAACGC	185	0.0	14.882845	3
CTCAACG	45	6.7917776E-4	14.768801	3
TATAACG	180	0.0	14.768801	2
GCCACGT	65	3.373705E-6	14.614003	8
TATCACG	130	0.0	14.606506	2
TAGGGTC	40	0.0052664103	14.25475	4
CTGGTCG	150	0.0	13.93142	9
CGCATAT	110	1.8553692E-10	13.816876	8
TAACGCA	215	0.0	13.702241	4
TTAGACT	70	7.2339135E-6	13.575953	4
GTTATCA	345	0.0	13.483981	1
CCCGCCC	50	0.0015123588	13.284538	13
ATCACGC	160	0.0	13.054565	3
GTTCTAG	80	2.012839E-6	13.054007	1
GAACAAA	1115	0.0	12.857106	1
TAAGACT	75	1.4727853E-5	12.670889	4
ACCGAGT	60	4.0939433E-4	12.665468	8
>>END_MODULE