FastQCFastQC Report
Thu 26 May 2016
SRR1364284_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364284_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1174058
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTT256002.1804714928904705No Hit
GTATCAACGCAGAGTACTTTTTTTT241982.061056608787641No Hit
TATCAACGCAGAGTACTTTTTTTTT188581.606223883317519No Hit
GGTATCAACGCAGAGTACTTTTTTT167931.430338194535534No Hit
GAGTACTTTTTTTTTTTTTTTTTTT114050.9714170850162428No Hit
ACGCAGAGTACTTTTTTTTTTTTTT71770.611298590018551No Hit
GTGGTATCAACGCAGAGTACTTTTT35740.30441426232775554No Hit
ATCAACGCAGAGTACTTTTTTTTTT28450.24232192958099172No Hit
GCAGAGTACTTTTTTTTTTTTTTTT27590.2349969081595628No Hit
GTATAACGCAGAGTACTTTTTTTTT27340.23286754146728694No Hit
GTGGTATCAACGCAGAGTACATGGG22580.19232439964635478No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA17800.15161090849004052No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT16900.14394518839784745No Hit
GATATAGGCTTACTAGGAGGGTGAA16030.1365349923087275No Hit
GGTATCAACGCAGAGTACATGGGAT15220.12963584422575375No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT12050.10263547456769598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG1100.018.1386412
TATAACG4350.016.3813672
AAGACGG350.002165486616.2905375
GTCTAGG350.002167953916.287761
CGGGTTC551.1301423E-515.54210916
TAACGCA4650.015.5294934
ATAACGC4900.015.1249923
ATCTACG456.754151E-414.77963352
GTATAAC5450.014.2953731
CGCGAGT400.00527368814.2517891
GTCCGAC400.00528861614.24571912
AACCGTC759.662508E-713.9338967
GCCTATT551.9588377E-413.818747
CCACGCA903.635796E-813.7221918
CGACGGG500.001501599713.29713715
GTATCAC1650.013.2440871
ACGGGTT655.4612887E-513.15101515
GGTATCA44600.012.8883911
TAGCCTA751.4733612E-512.6704175
TCACGCA1501.8189894E-1212.66879754
Produced by FastQC (version 0.11.5)