##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364284_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1174058 Sequences flagged as poor quality 0 Sequence length 25 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.366206780244248 31.0 30.0 34.0 25.0 34.0 2 29.331539838747318 31.0 30.0 34.0 25.0 34.0 3 29.370229579799293 31.0 29.0 34.0 25.0 34.0 4 32.683045471348095 35.0 33.0 37.0 26.0 37.0 5 32.30256000981212 35.0 32.0 37.0 24.0 37.0 6 32.12923126455422 35.0 32.0 37.0 22.0 37.0 7 31.847045887000473 35.0 32.0 37.0 17.0 37.0 8 31.69492733749099 35.0 32.0 37.0 17.0 37.0 9 32.35095795948752 37.0 32.0 39.0 10.0 39.0 10 32.5025416120839 37.0 32.0 39.0 13.0 39.0 11 32.401361772587045 37.0 32.0 39.0 10.0 39.0 12 32.381910433726446 37.0 32.0 39.0 10.0 39.0 13 32.38807963490731 37.0 32.0 39.0 10.0 39.0 14 33.239743692389986 38.0 32.0 41.0 10.0 41.0 15 33.28222626139424 38.0 32.0 41.0 10.0 41.0 16 33.269236272824685 38.0 32.0 41.0 10.0 41.0 17 33.33381059538796 38.0 32.0 41.0 10.0 41.0 18 33.315549998381684 38.0 32.0 41.0 10.0 41.0 19 33.26912554575668 39.0 32.0 41.0 8.0 41.0 20 33.160078122205206 39.0 32.0 41.0 2.0 41.0 21 33.096869149565016 38.0 32.0 41.0 2.0 41.0 22 32.98386195571258 38.0 32.0 41.0 2.0 41.0 23 32.70980990717665 38.0 32.0 41.0 2.0 41.0 24 32.62568459139157 38.0 32.0 41.0 2.0 41.0 25 32.45814346480327 38.0 32.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.34534656911299777 1101 2 0.4464428558548619 1101 3 -1.253642011186745 1101 4 -0.2262638082365953 1101 5 0.45145892353091455 1101 6 0.6896218190859997 1101 7 0.7049746315291117 1101 8 0.7910863869879314 1101 9 1.491559083926461 1101 10 1.1691603211846413 1101 11 1.300841584030458 1101 12 1.3326961265213058 1101 13 1.1872618282404233 1101 14 1.4400804520137171 1101 15 1.3461768165641104 1101 16 1.3015025867539052 1101 17 1.2235217284394224 1101 18 1.2314756955023682 1101 19 1.2221506769884911 1101 20 1.4465027046176928 1101 21 1.4090921400571759 1101 22 1.3749362699160272 1101 23 1.6984610613663307 1101 24 1.7427566308050828 1101 25 1.7967124636902838 1102 1 0.8268215168304494 1102 2 0.8178717412141623 1102 3 0.8192550584268687 1102 4 0.8031157477921056 1102 5 0.9607522759256568 1102 6 1.1171090094042384 1102 7 1.2676871342912648 1102 8 1.3408402652968725 1102 9 1.8280660970517317 1102 10 1.4834211564601318 1102 11 1.449161339569109 1102 12 1.5644427115218065 1102 13 1.5089975721664288 1102 14 1.511742335015569 1102 15 1.7617372988942606 1102 16 1.6796515953693856 1102 17 1.6805148319045387 1102 18 1.6199713377078453 1102 19 1.5371147170955837 1102 20 1.8167244827248297 1102 21 1.8844915689474178 1102 22 2.01944822012522 1102 23 2.5356972222097838 1102 24 2.4095345975420273 1102 25 2.2417233108689842 1103 1 -2.292696187493032 1103 2 -2.262705995559294 1103 3 -2.174212225728546 1103 4 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0.38300868253240594 1104 17 0.351104595487044 1104 18 0.6079820520693247 1104 19 0.6722633618602174 1104 20 1.0582605643571696 1104 21 0.8939257752177667 1104 22 0.6848697021468126 1104 23 1.0930570394018773 1104 24 1.1925542468347174 1104 25 1.2304091218378588 1105 1 0.39743146662204865 1105 2 0.45741075343037707 1105 3 -0.06378820879352531 1105 4 0.21795019808001115 1105 5 0.37482826265077307 1105 6 0.49640478247382447 1105 7 0.36177642656690523 1105 8 0.2754062690254919 1105 9 0.3954345882577428 1105 10 0.15041790615476458 1105 11 0.4574156761110686 1105 12 0.7562537821408952 1105 13 0.5213562228090325 1105 14 0.3789988260422845 1105 15 0.3535273141873816 1105 16 0.42273533363930227 1105 17 0.4973308419733513 1105 18 0.5262636363537609 1105 19 0.7491433240292196 1105 20 0.9137128633697316 1105 21 0.9732612950453472 1105 22 0.7812057323647323 1105 23 1.188746145468727 1105 24 1.0998293308205902 1105 25 1.3018757820977953 1106 1 0.010028069237858261 1106 2 0.2504304086770226 1106 3 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1.264173567020336 2210 23 1.454239598179754 2210 24 1.3479838588635076 2210 25 1.361887962577896 2211 1 -0.1315274843591645 2211 2 0.07774152931149914 2211 3 0.17984968344345376 2211 4 -0.061511501890031184 2211 5 -0.6168621868630382 2211 6 -0.09925847788728959 2211 7 0.08765762882237027 2211 8 0.25401832181981376 2211 9 0.6775173398274603 2211 10 0.16337224253440752 2211 11 0.4049218140517681 2211 12 0.4957519912966575 2211 13 0.32345865788089156 2211 14 0.44450656236754327 2211 15 0.5008215526368929 2211 16 0.5265156848075989 2211 17 0.4177801951703657 2211 18 0.37613151763022756 2211 19 0.04555366024935381 2211 20 0.41753021110366717 2211 21 0.7573054738185121 2211 22 0.54534653420437 2211 23 0.9889558295966978 2211 24 0.9859671385968483 2211 25 1.1718619305393148 2212 1 -0.14036538742122318 2212 2 -0.09053796252715784 2212 3 -0.11690437888685778 2212 4 -0.15369492273613616 2212 5 -0.3523910634945686 2212 6 -0.15272918638167 2212 7 -0.14348587886313524 2212 8 -0.31946166580112845 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-0.5011431606937968 2215 6 -0.1016938293203431 2215 7 -0.42485909278077827 2215 8 -0.4101792459268836 2215 9 0.29299709078207314 2215 10 0.2805357717912713 2215 11 0.5791468113132439 2215 12 0.46332286931775 2215 13 0.2721295601337843 2215 14 -0.04910990684866334 2215 15 -0.3148256079823355 2215 16 -0.0050033614994831055 2215 17 -0.0553048009182433 2215 18 -0.08686159677596805 2215 19 -0.16425833858663452 2215 20 0.013507234044212169 2215 21 -0.05547038422517403 2215 22 0.06381360163627647 2215 23 0.21801357763243345 2215 24 0.5525268644566097 2215 25 0.6276040377766563 2216 1 -1.0081760636540231 2216 2 -0.8871332401413383 2216 3 -0.9015319957418129 2216 4 -0.9190640933738159 2216 5 -1.8716752097707392 2216 6 -2.2975945287814135 2216 7 -1.7843671767161098 2216 8 -2.3047280362032296 2216 9 -1.1442933575610041 2216 10 -1.612434071116656 2216 11 -1.305507471452497 2216 12 -2.273271557926833 2216 13 -1.892404323595148 2216 14 -2.125870599429792 2216 15 -1.7970868165593288 2216 16 -2.790334172186334 2216 17 -3.11429459566655 2216 18 -2.596910903048183 2216 19 -4.341477694165125 2216 20 -2.888739067985373 2216 21 -2.973999222661135 2216 22 -2.6379281710962097 2216 23 -2.3716458650920273 2216 24 -2.657519001843127 2216 25 -2.7069539257596382 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 49281.0 3 3.0 4 649.0 5 6741.0 6 10124.0 7 8046.0 8 6113.0 9 4697.0 10 3972.0 11 3657.0 12 3200.0 13 2811.0 14 2891.0 15 2814.0 16 2911.0 17 2967.0 18 3551.0 19 4599.0 20 6744.0 21 8717.0 22 10387.0 23 11708.0 24 13356.0 25 14878.0 26 16299.0 27 17197.0 28 19065.0 29 22023.0 30 26559.0 31 33203.0 32 40796.0 33 51111.0 34 63216.0 35 80192.0 36 107407.0 37 165927.0 38 346246.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.963179019419236 19.4111437609746 13.445306556090694 21.180370663515465 2 15.236089154787539 23.401513396385837 40.38395363732999 20.978443811496632 3 17.200527742690515 32.02629855279599 30.269654710120548 20.503518994392948 4 10.606979497619271 18.873689321215323 38.23979352816416 32.27953765300125 5 10.493674110843857 40.88282130651828 34.57260948359376 14.050895099044109 6 25.378385193059927 40.205769309400175 20.137469163565985 14.278376333973913 7 24.02326395662756 34.86194373475613 24.471198202810793 16.643594105805512 8 25.749630456359775 33.800697766550655 22.224333223996695 18.22533855309288 9 26.73798796240125 15.549701104923418 22.136895308335664 35.57541562433967 10 15.121633251250415 31.60743347449323 32.436499348165064 20.834433926091293 11 30.378062040076216 25.45229744607026 25.407647133006805 18.761993380846718 12 22.442436234996283 30.25770065223743 30.678924902828804 16.620938209937485 13 27.4327358461638 23.36162340250829 29.27519616227211 19.9304445890558 14 21.355456348952835 22.912295854873292 32.42037136887224 23.311876427301634 15 22.227257317403502 29.90071398028249 28.257758636844514 19.614270065469487 16 19.639868172568207 29.37002058845326 29.870293596284025 21.119817642694507 17 18.87652796115678 28.890838621747093 31.137441969419484 21.095191447676648 18 18.07464464060272 25.914188990034777 36.13744822733461 19.873718142027904 19 19.866491858916856 26.035024589793853 36.249181121103774 17.849302430185514 20 20.004531983279367 25.128143530977216 36.46074254671904 18.40658193902438 21 21.374971782454434 24.321863167265093 35.83206620581558 18.47109884446489 22 20.124930575195158 24.88074266652583 36.08709874166476 18.90722801661425 23 19.686447210947517 24.968376399637126 36.38583773792234 18.95933865149302 24 19.33351902533844 25.205560197855682 36.39444048358287 19.06648029322301 25 19.24252294532345 24.884974948584055 36.82762784066686 19.044874265425634 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2676.0 1 2676.0 2 3710.5 3 4745.0 4 4745.0 5 4745.0 6 17050.5 7 29356.0 8 29356.0 9 29356.0 10 23717.5 11 18079.0 12 18079.0 13 18079.0 14 14492.5 15 10906.0 16 10906.0 17 10906.0 18 19231.0 19 27556.0 20 27556.0 21 27556.0 22 38343.0 23 49130.0 24 49130.0 25 49130.0 26 69960.0 27 90790.0 28 90790.0 29 90790.0 30 101415.5 31 112041.0 32 112041.0 33 112041.0 34 115570.5 35 119100.0 36 119100.0 37 119100.0 38 117646.5 39 116193.0 40 116193.0 41 116193.0 42 119250.0 43 122307.0 44 122307.0 45 122307.0 46 126723.0 47 131139.0 48 131139.0 49 131139.0 50 122236.0 51 113333.0 52 113333.0 53 113333.0 54 99250.0 55 85167.0 56 85167.0 57 85167.0 58 72767.5 59 60368.0 60 60368.0 61 60368.0 62 49565.0 63 38762.0 64 38762.0 65 38762.0 66 29728.0 67 20694.0 68 20694.0 69 20694.0 70 15829.5 71 10965.0 72 10965.0 73 10965.0 74 8001.0 75 5037.0 76 5037.0 77 5037.0 78 4166.5 79 3296.0 80 3296.0 81 3296.0 82 2312.0 83 1328.0 84 1328.0 85 1328.0 86 971.5 87 615.0 88 615.0 89 615.0 90 527.0 91 439.0 92 439.0 93 439.0 94 235.5 95 32.0 96 32.0 97 32.0 98 18.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02768176699958605 2 8.5174667691034E-4 3 8.517466769103401E-5 4 0.004088384049169632 5 0.033132945731812226 6 0.010391309458306148 7 0.019164300230482652 8 0.026659670987293643 9 0.03424021641179567 10 0.03815825112558323 11 0.0420762858393708 12 0.008432292101412366 13 0.00255524003073102 14 0.006558449412209619 15 0.006473274744518584 16 0.008432292101412366 17 0.009198864110631672 18 0.014990741513621985 19 0.0167794095351337 20 0.015246265516695088 21 0.012179977479817862 22 0.010732008129070283 23 0.007921244095266162 24 0.006047401406063415 25 0.10817182796761318 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1174058.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.17161999698413 #Duplication Level Percentage of deduplicated Percentage of total 1 77.95722423666372 31.31667988054935 2 11.099063493568213 8.917347219720426 3 3.914550485993732 4.717615037470491 4 1.9482918222872052 3.1306415491261332 5 1.0474712948622427 2.103930940747746 6 0.6980736682106229 1.6825650077554748 7 0.4921006026509593 1.383793488698685 8 0.34815453681793723 1.1188745402620954 9 0.2614970312797363 0.9454283433818134 >10 1.8522217294564687 14.819770464402152 >50 0.2289845597311896 6.446237085802088 >100 0.1405158077695405 10.389140991922886 >500 0.007618326884017873 1.9601896112874624 >1k 0.002962682677118062 2.22397954813476 >5k 2.1162019122271869E-4 0.6101258062368861 >10k+ 0.0010581009561135934 8.233680484501512 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACTTTTTTTTTTTTTTTTTTTTT 25600 2.1804714928904705 No Hit GTATCAACGCAGAGTACTTTTTTTT 24198 2.061056608787641 No Hit TATCAACGCAGAGTACTTTTTTTTT 18858 1.606223883317519 No Hit GGTATCAACGCAGAGTACTTTTTTT 16793 1.430338194535534 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 11405 0.9714170850162428 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 7177 0.611298590018551 No Hit GTGGTATCAACGCAGAGTACTTTTT 3574 0.30441426232775554 No Hit ATCAACGCAGAGTACTTTTTTTTTT 2845 0.24232192958099172 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 2759 0.2349969081595628 No Hit GTATAACGCAGAGTACTTTTTTTTT 2734 0.23286754146728694 No Hit GTGGTATCAACGCAGAGTACATGGG 2258 0.19232439964635478 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 1780 0.15161090849004052 No Hit ACTTTTTTTTTTTTTTTTTTTTTTT 1690 0.14394518839784745 No Hit GATATAGGCTTACTAGGAGGGTGAA 1603 0.1365349923087275 No Hit GGTATCAACGCAGAGTACATGGGAT 1522 0.12963584422575375 No Hit CTTTTTTTTTTTTTTTTTTTTTTTT 1205 0.10263547456769598 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 8.517466769103401E-5 0.0 3 0.0 0.0 0.0 8.517466769103401E-5 0.0 4 0.0 0.0 0.0 8.517466769103401E-5 0.0 5 0.0 0.0 0.0 8.517466769103401E-5 0.0 6 0.0 0.0 0.0 8.517466769103401E-5 0.0 7 0.0 0.0 0.0 8.517466769103401E-5 0.0 8 0.0 0.0 0.0 8.517466769103401E-5 0.0 9 0.0 0.0 0.0 8.517466769103401E-5 0.0 10 0.0 0.0 0.0 8.517466769103401E-5 0.0 11 0.0 0.0 0.0 8.517466769103401E-5 0.0 12 0.0 0.0 0.0 8.517466769103401E-5 0.0 13 0.0 0.0 0.0 8.517466769103401E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 110 0.0 18.138641 2 TATAACG 435 0.0 16.381367 2 AAGACGG 35 0.0021654866 16.290537 5 GTCTAGG 35 0.0021679539 16.28776 1 CGGGTTC 55 1.1301423E-5 15.542109 16 TAACGCA 465 0.0 15.529493 4 ATAACGC 490 0.0 15.124992 3 ATCTACG 45 6.754151E-4 14.7796335 2 GTATAAC 545 0.0 14.295373 1 CGCGAGT 40 0.005273688 14.251789 1 GTCCGAC 40 0.005288616 14.245719 12 AACCGTC 75 9.662508E-7 13.933896 7 GCCTATT 55 1.9588377E-4 13.81874 7 CCACGCA 90 3.635796E-8 13.722191 8 CGACGGG 50 0.0015015997 13.297137 15 GTATCAC 165 0.0 13.244087 1 ACGGGTT 65 5.4612887E-5 13.151015 15 GGTATCA 4460 0.0 12.888391 1 TAGCCTA 75 1.4733612E-5 12.670417 5 TCACGCA 150 1.8189894E-12 12.6687975 4 >>END_MODULE