FastQCFastQC Report
Thu 26 May 2016
SRR1364283_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364283_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4675878
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN123090.26324467832565346No Hit
GTATCAACGCAGAGTACTTTTTTTT82690.17684379275934914No Hit
TATCAACGCAGAGTACTTTTTTTTT49630.10614049382811101No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGCG1650.012.10120816
TTCGCGC1650.012.10042817
GGTATCA33650.011.9052881
TTTAGCG802.8900304E-511.8666252
TCGCGCA1657.2759576E-1211.52409318
CGCGCCA2200.011.22658210
TACCGTA951.3611718E-511.0003857
GTATCAA77650.010.4162321
GCGCGGT1106.14043E-610.35110811
TCGCGGT1855.820766E-1110.27835517
GTATTAG5550.010.2629171
CTTCGCG1951.4551915E-1110.23904315
TGCCGTA1409.590258E-810.18680315
GTGGTAT13450.010.1637211
CGCCAGT2250.010.121312
GGACCGA2450.010.0820946
TAGCGCG856.5707404E-410.063394
TGGTATC12400.09.8760942
ACCGAGT2700.09.8519858
CGCAGAT8900.09.8203588
Produced by FastQC (version 0.11.5)