##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364283_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4675878
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.8197666833908	31.0	26.0	31.0	10.0	34.0
2	26.676976388177792	31.0	26.0	31.0	10.0	34.0
3	26.03421218432132	30.0	25.0	31.0	10.0	34.0
4	29.96411304999831	35.0	28.0	37.0	10.0	37.0
5	30.484315245179623	35.0	30.0	37.0	10.0	37.0
6	30.84720024773957	35.0	31.0	37.0	11.0	37.0
7	30.387914740290487	35.0	30.0	37.0	10.0	37.0
8	29.948374401556244	35.0	30.0	37.0	8.0	37.0
9	31.14846794548532	37.0	30.0	39.0	8.0	39.0
10	30.408784617562734	35.0	28.0	39.0	7.0	39.0
11	31.12844197389239	37.0	30.0	39.0	7.0	39.0
12	30.626054400906096	35.0	29.0	39.0	2.0	39.0
13	30.28566356949433	35.0	27.0	39.0	2.0	39.0
14	30.618149147603937	36.0	27.0	40.0	2.0	41.0
15	31.344026084512898	37.0	29.0	40.0	2.0	41.0
16	31.71868363545841	37.0	30.0	40.0	2.0	41.0
17	31.87707228460623	37.0	31.0	40.0	2.0	41.0
18	31.708429732341177	37.0	30.0	40.0	2.0	41.0
19	31.674913887830265	38.0	30.0	40.0	2.0	41.0
20	31.539354106330403	38.0	30.0	40.0	2.0	41.0
21	31.461834975164024	38.0	30.0	40.0	2.0	41.0
22	31.54956202877834	38.0	30.0	40.0	2.0	41.0
23	31.556765809544217	38.0	31.0	40.0	2.0	41.0
24	31.38386651661998	38.0	30.0	40.0	2.0	41.0
25	31.208578795255136	38.0	30.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.29028545287492946
1101	2	0.4548802558048415
1101	3	1.2569833907787142
1101	4	0.560765236958833
1101	5	0.04379263819242496
1101	6	-0.14061910378181608
1101	7	0.15612981933123038
1101	8	0.45367228866633624
1101	9	0.3445880616303505
1101	10	0.6662769444457197
1101	11	0.10455035376955735
1101	12	0.42802407192817427
1101	13	0.3403030628665853
1101	14	0.8844951880220329
1101	15	0.1629503309772531
1101	16	-0.45670022197758087
1101	17	-1.0528795482176
1101	18	-6.966992791885776
1101	19	-8.315905087759493
1101	20	-9.400516733205595
1101	21	-11.019136632809925
1101	22	-11.335376817719983
1101	23	-12.698205240239133
1101	24	-12.195367797555672
1101	25	-12.633877871914795
1102	1	1.7091569300410292
1102	2	2.0034627388358786
1102	3	2.6922874468444498
1102	4	2.040738761055728
1102	5	1.406364038199822
1102	6	1.4186620980135842
1102	7	1.6742373563764978
1102	8	1.9607124719919078
1102	9	2.079581445788559
1102	10	-1.5978446341047885
1102	11	0.7065639076267729
1102	12	1.7984637323797337
1102	13	2.317380393313311
1102	14	2.900731814525642
1102	15	2.483594969700892
1102	16	2.0368399303215448
1102	17	1.7032353261127469
1102	18	1.9308426745420384
1102	19	1.6022231649249186
1102	20	1.9504718509415788
1102	21	2.062893615784013
1102	22	1.9110676765896386
1102	23	1.9576518951859896
1102	24	2.0792105561370704
1102	25	2.15029274722896
1103	1	-1.40991903593547
1103	2	-0.9177379143013162
1103	3	-3.6646910180735333
1103	4	-1.582495819163185
1103	5	-4.6007432305715845
1103	6	-8.598535155288786
1103	7	-9.654860157258476
1103	8	-3.7142679297000463
1103	9	-9.268003455758766
1103	10	-6.475662283900839
1103	11	-10.33054606508232
1103	12	-4.195509001867212
1103	13	-1.8917393177636121
1103	14	-1.4701745495794825
1103	15	-1.8251218756212957
1103	16	-1.8913616157439144
1103	17	-1.9065403518629473
1103	18	-1.8729630653517297
1103	19	-8.179716837003468
1103	20	-3.237047275749429
1103	21	-8.71890123290028
1103	22	-4.458260866511964
1103	23	-3.4319153070978956
1103	24	-8.93306982752863
1103	25	-6.125344354988265
1104	1	1.695235516851298
1104	2	1.6948221837409818
1104	3	2.6241577181654563
1104	4	1.9833135011397403
1104	5	1.6765745374997252
1104	6	1.4561805115768962
1104	7	1.5803465590578156
1104	8	1.8353193708896747
1104	9	2.020579225530451
1104	10	2.3521745008146198
1104	11	1.8977929769103703
1104	12	2.284089611295176
1104	13	2.548045848101747
1104	14	3.07841085510686
1104	15	2.0945918534425907
1104	16	1.3856141564962918
1104	17	1.113125194021059
1104	18	1.0533800407874345
1104	19	1.5303723018793143
1104	20	-9.47744311104675
1104	21	-3.481811706457144
1104	22	-0.9166934467212471
1104	23	0.16814177949565945
1104	24	0.7987420695269591
1104	25	1.3447841684067612
1105	1	1.4067766146670806
1105	2	1.3535642155927796
1105	3	2.13733310945862
1105	4	1.550752511160141
1105	5	1.350231841471924
1105	6	1.2159386002533275
1105	7	1.5724765662741085
1105	8	1.7480377175966346
1105	9	1.5984633351348911
1105	10	2.296893631532683
1105	11	1.8391138661853539
1105	12	2.0207982356483463
1105	13	2.108602696223084
1105	14	2.8018052304048098
1105	15	2.1022204817081125
1105	16	1.6139894282624176
1105	17	0.8796525408366094
1105	18	1.470073508619489
1105	19	1.3412852666350084
1105	20	1.3631636195884447
1105	21	1.2522696720469355
1105	22	1.2688279168111478
1105	23	1.255528288497537
1105	24	1.3281106017241697
1105	25	1.4073941167221022
1106	1	1.036614803526568
1106	2	1.3593380765866812
1106	3	2.017173194584739
1106	4	1.5215332095497232
1106	5	0.7773737558993687
1106	6	0.504754365489017
1106	7	1.0384645862033288
1106	8	0.9856060644638944
1106	9	1.2423512343531975
1106	10	-2.432992939215403
1106	11	-0.2575258409877321
1106	12	0.43853141684692076
1106	13	1.433375740196965
1106	14	0.5339545890332538
1106	15	1.299251253239408
1106	16	0.7648566722610006
1106	17	-6.273011009451299
1106	18	-1.9872041459912566
1106	19	-0.2814841820173406
1106	20	0.507130349319521
1106	21	0.7872132608236413
1106	22	0.8367827986683167
1106	23	0.32444972454567633
1106	24	0.731011456236093
1106	25	0.8390992613476485
1107	1	0.7806931502608414
1107	2	1.0613301173514706
1107	3	1.6160678845347363
1107	4	1.0672051002288718
1107	5	0.42276593290552
1107	6	0.6407561003038857
1107	7	0.5125782682485145
1107	8	0.7899589852451676
1107	9	0.9438923730692892
1107	10	-2.4685537393704777
1107	11	-0.17982056628221343
1107	12	0.21095246197962325
1107	13	1.1815585262105799
1107	14	1.6045074213921708
1107	15	1.306136089538807
1107	16	-5.690700468089005
1107	17	-2.0502712066099207
1107	18	-0.1775040726728534
1107	19	0.5030944787202536
1107	20	0.9287970679633695
1107	21	1.0616072986128842
1107	22	0.6604654217181789
1107	23	0.3367974390478601
1107	24	0.562314316265212
1107	25	0.8568052881935557
1108	1	-0.5125292542826791
1108	2	-3.6221765772211754
1108	3	-0.30748023474651376
1108	4	-0.752142445265843
1108	5	-0.8788577649862717
1108	6	-0.48948147798027364
1108	7	-0.12820865850873275
1108	8	0.13140445914384813
1108	9	-0.4119771541364692
1108	10	-3.537513810545601
1108	11	-1.5137382855417343
1108	12	-6.313943832391757
1108	13	-1.878265045900232
1108	14	-6.225815366332977
1108	15	-2.788372696754518
1108	16	-7.99445616665259
1108	17	-3.552226186317629
1108	18	-2.0798442595121607
1108	19	-0.736890400555243
1108	20	-0.7323788223222252
1108	21	-0.18665210712913805
1108	22	-0.41437349476697705
1108	23	-0.3178530737317544
1108	24	0.01928529403395629
1108	25	-0.2488129686005074
1109	1	-9.199883714308655
1109	2	-6.731341070772206
1109	3	-5.647280734593249
1109	4	-5.582935535270508
1109	5	-2.6998118365942005
1109	6	-1.5542893019199333
1109	7	-1.0499242021930293
1109	8	-0.4951738376699879
1109	9	-5.655055697950068
1109	10	-1.4820410026722328
1109	11	-1.1257718719330256
1109	12	-6.271856708538937
1109	13	-8.375686844631144
1109	14	-8.377671786533366
1109	15	-4.037131538034796
1109	16	-2.257218304377485
1109	17	-1.9040025764862243
1109	18	-1.24097967249606
1109	19	-0.9513448897570278
1109	20	-0.6559379539741528
1109	21	-0.5840449937771979
1109	22	-0.8213581174872786
1109	23	-1.1452905122643777
1109	24	-0.8132835305877748
1109	25	-0.7366064484699528
1110	1	-2.5053778206402555
1110	2	-2.300842871615327
1110	3	-1.520043420889884
1110	4	-2.3994503045228655
1110	5	-5.9096929400198945
1110	6	-4.095255559498966
1110	7	-9.353058429321443
1110	8	-10.340838804839684
1110	9	-11.720095222948459
1110	10	-9.243303434629013
1110	11	-12.324535180923792
1110	12	-6.805929917489358
1110	13	-4.96147684761306
1110	14	-4.639437562913088
1110	15	-10.29196669949675
1110	16	-11.947238753886637
1110	17	-12.605953611678675
1110	18	-9.206425191144728
1110	19	-12.634451753425019
1110	20	-13.757232675398921
1110	21	-14.731174607706837
1110	22	-15.403807790353603
1110	23	-16.134879241792945
1110	24	-16.59669911021954
1110	25	-17.35101231641202
1111	1	-0.5042771704703846
1111	2	0.2803311297456723
1111	3	-2.5954813307049847
1111	4	-3.2516557373753585
1111	5	-0.9852660758470329
1111	6	-0.3951498254281667
1111	7	0.11486491566181556
1111	8	0.494650559310422
1111	9	-4.163845188814268
1111	10	-4.059703975969978
1111	11	-1.1827212699756195
1111	12	-5.7933014136373835
1111	13	-1.0603115336312925
1111	14	0.4206097589091655
1111	15	0.3387582233417703
1111	16	0.23263317826878804
1111	17	0.2198051231243241
1111	18	0.4761056761768536
1111	19	0.6772711839868926
1111	20	0.690563511984859
1111	21	0.8110560498578678
1111	22	0.33578650612342287
1111	23	0.27607058804583673
1111	24	0.4940228525019208
1111	25	0.7321559955530041
1112	1	-0.15498111174136397
1112	2	0.4534489366267209
1112	3	-2.4897564943773105
1112	4	-0.44307592043731603
1112	5	-0.4196977138013658
1112	6	-0.1492990326383321
1112	7	0.3219044362019723
1112	8	-3.2885358837886933
1112	9	-0.4877327226247985
1112	10	-3.2282818504994104
1112	11	-0.7211653981217339
1112	12	0.4772935228352715
1112	13	0.8253026040475717
1112	14	1.506577697141303
1112	15	0.9966867622606621
1112	16	0.5772478275570272
1112	17	0.3982380164433792
1112	18	0.5040693237475402
1112	19	0.5944423886090213
1112	20	0.6917634811417237
1112	21	0.6665099624030191
1112	22	0.6037791852826047
1112	23	0.36040983091550416
1112	24	0.6162771328326784
1112	25	0.7011668473629662
1113	1	-1.0941149547004834
1113	2	-0.6282344414826291
1113	3	-3.350391953132206
1113	4	-1.4929185056813665
1113	5	-1.6531508720546526
1113	6	-1.5302588219904933
1113	7	-1.331457282087655
1113	8	-9.008244787831106
1113	9	-10.002030925511015
1113	10	-3.6681383561929017
1113	11	-9.558258619789793
1113	12	-7.892386669938045
1113	13	-3.1546236161106584
1113	14	-1.4850394698802774
1113	15	-7.626009117339219
1113	16	-3.355505307623236
1113	17	-2.4198169287328746
1113	18	-1.8695017842675057
1113	19	-1.554058873108474
1113	20	-7.362014906361512
1113	21	-3.0393363800303668
1113	22	-2.358041395652105
1113	23	-2.2473078804706432
1113	24	-7.999750766638286
1113	25	-5.003037423965189
1114	1	-4.3400280336405395
1114	2	-4.465524977297118
1114	3	-0.9297962371298425
1114	4	-0.5238663974891011
1114	5	-0.5523715173666588
1114	6	-0.7004220136570147
1114	7	-0.49725441289035643
1114	8	-0.140027885065237
1114	9	-0.45222632565148757
1114	10	-3.4782275779752716
1114	11	-1.3527577639432806
1114	12	-4.916596302267987
1114	13	-1.3825073159223251
1114	14	-6.120659685952482
1114	15	-2.208020368939689
1114	16	-1.2208405218417937
1114	17	-1.099052744854749
1114	18	-0.5514203491531688
1114	19	-0.3079377433345911
1114	20	-0.13049965578439426
1114	21	0.026875080340712287
1114	22	-0.01178513078002652
1114	23	-0.516449536620879
1114	24	-0.35999278984540695
1114	25	-0.15614830553857217
1115	1	1.0127300575371976
1115	2	1.148401237964336
1115	3	1.8325023484776253
1115	4	0.793744306876679
1115	5	0.5784435896314477
1115	6	-0.021631329655754428
1115	7	-1.9817940346100222
1115	8	0.1776887524719939
1115	9	0.37925341352217146
1115	10	1.2341772891874037
1115	11	0.28104064594922207
1115	12	1.0722870346559716
1115	13	1.3169144440710987
1115	14	2.000007002212442
1115	15	1.017893612344718
1115	16	0.6806792506520587
1115	17	0.32217170045265675
1115	18	0.6879374811954229
1115	19	0.9151793466253402
1115	20	0.9121156607271494
1115	21	1.0438143389149488
1115	22	0.6062562319305655
1115	23	0.5484892481514478
1115	24	0.41598950441674276
1115	25	0.7664357117561096
1116	1	0.5878366805482287
1116	2	0.90233719870022
1116	3	1.714626221686018
1116	4	1.054003738205754
1116	5	0.29267094172786656
1116	6	-0.8676890195092071
1116	7	-4.5336943603101645
1116	8	-0.7258461621152854
1116	9	-0.8472549234237476
1116	10	-0.08517074613754971
1116	11	-1.8553169247729144
1116	12	-0.13000998112434203
1116	13	-6.513036707729633
1116	14	-1.4384436643808378
1116	15	-0.8211229371545805
1116	16	-0.40991208081170427
1116	17	-0.029908561821926583
1116	18	0.01575660538740209
1116	19	0.3536554208446283
1116	20	0.4120471250287032
1116	21	0.5215843966050713
1116	22	0.26265378064540457
1116	23	0.2393115011586282
1116	24	0.39621826156985307
1116	25	0.47676950739619883
1201	1	2.045929240045087
1201	2	2.237121832950592
1201	3	2.963404308481337
1201	4	2.471262812745696
1201	5	1.8798633626981314
1201	6	1.7127611960888274
1201	7	2.046399556491263
1201	8	2.077755010695089
1201	9	2.4467787318254572
1201	10	2.7371535304112413
1201	11	2.276522624027219
1201	12	2.686770874389527
1201	13	2.800583263020105
1201	14	3.4463661260261063
1201	15	2.8670423215131997
1201	16	2.366564814782574
1201	17	2.1291998581484037
1201	18	2.2881533729382433
1201	19	2.225877649312725
1201	20	2.3883882792859374
1201	21	2.3726772668683154
1201	22	1.3947220613413265
1201	23	0.9524541595411407
1201	24	1.4493394401161126
1201	25	1.5391891434219929
1202	1	2.187804640206142
1202	2	2.3610037360236795
1202	3	3.1376136150844722
1202	4	2.6655694506325425
1202	5	2.115575499206088
1202	6	1.861172843303855
1202	7	2.1655751054115733
1202	8	2.316651680776541
1202	9	2.640489804641309
1202	10	-1.2396925087179795
1202	11	1.2529165726543177
1202	12	2.3169893055869473
1202	13	2.8832999048624934
1202	14	3.363303902678176
1202	15	2.9349298862209032
1202	16	2.587462609906563
1202	17	2.2040811771110853
1202	18	2.362423925126617
1202	19	2.627012333181167
1202	20	2.478305461260579
1202	21	2.737218829897742
1202	22	2.4303074043832
1202	23	2.1303681581276734
1202	24	2.3327365901869825
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2211	20	0.8887717435480091
2211	21	0.9608011929650928
2211	22	0.8174952745916357
2211	23	0.7215649392859902
2211	24	0.8940824560594223
2211	25	0.856437489734649
2212	1	1.174055066806858
2212	2	1.2492602131095687
2212	3	1.977534192765038
2212	4	1.4475015422780189
2212	5	0.9812219246997245
2212	6	0.7522833886599543
2212	7	1.1917514606703357
2212	8	1.419359812973525
2212	9	1.2329131480616304
2212	10	1.9900960162371888
2212	11	1.2084794994018928
2212	12	1.6142760146631652
2212	13	1.6868587214327668
2212	14	-4.427190162365072
2212	15	-0.8752899360639823
2212	16	-0.17810010109302254
2212	17	0.3615278686654335
2212	18	0.689606221430072
2212	19	1.0892039924491463
2212	20	1.4520866862435078
2212	21	1.3304240639868112
2212	22	1.2777360656711636
2212	23	1.1751968887540514
2212	24	1.2803314913569537
2212	25	1.3699850021288498
2213	1	0.7615609503463396
2213	2	0.9223432979817261
2213	3	1.5571275057518967
2213	4	0.9755223490017784
2213	5	0.41416103244047164
2213	6	0.15976611185709189
2213	7	0.4333709108694137
2213	8	-5.444402237931506
2213	9	-1.758958830445529
2213	10	0.21201251259695297
2213	11	0.07295969194192153
2213	12	0.5535470039853543
2213	13	-8.229263755151539
2213	14	-2.3104128460621354
2213	15	-0.7626151443275901
2213	16	-0.30287233891192145
2213	17	-0.11006650749889602
2213	18	0.26919987240570364
2213	19	0.6035907209551432
2213	20	0.7736880520155616
2213	21	0.7377383166824885
2213	22	0.3737347281820149
2213	23	0.2662737081256239
2213	24	0.36081572583706745
2213	25	0.46050439352464423
2214	1	-0.8191114361107772
2214	2	-0.4003285590308323
2214	3	-7.2587308531895545
2214	4	-2.4494776645528695
2214	5	-1.4528569910397025
2214	6	-1.070957263797922
2214	7	-0.5868588424508978
2214	8	-6.42410559242229
2214	9	-1.960123296718379
2214	10	-0.06285514998393538
2214	11	-0.4791602855356736
2214	12	-0.16024412539200128
2214	13	-6.562721554042035
2214	14	-1.5775416177954895
2214	15	-0.7173673734911468
2214	16	-0.6875501544122855
2214	17	-0.5243543164618529
2214	18	-0.45628307293260306
2214	19	-0.15859897417777802
2214	20	0.1377493831389458
2214	21	0.19518961517034583
2214	22	-0.02378753973292902
2214	23	-0.17078336832923213
2214	24	0.054218885262908856
2214	25	0.10406538327421444
2215	1	0.30125944630180257
2215	2	0.5865895073264866
2215	3	-2.560534279813254
2215	4	-0.48371422637749717
2215	5	0.0435036456875757
2215	6	-0.5201133577239645
2215	7	0.1724724983865542
2215	8	0.5735518842970144
2215	9	0.8872703432705187
2215	10	1.44495409883897
2215	11	0.6314612899493106
2215	12	0.4696545125943459
2215	13	1.050490140406179
2215	14	1.2682335033211132
2215	15	0.8958706445815245
2215	16	0.613297661802175
2215	17	0.454044880395287
2215	18	0.6942850565176251
2215	19	0.5806151825417736
2215	20	0.8585113773552884
2215	21	0.9706089498096659
2215	22	0.7400672750744377
2215	23	0.5973836332130347
2215	24	0.4320811081107543
2215	25	0.5822921150056182
2216	1	0.38796362480177393
2216	2	0.18789018506601352
2216	3	1.4901957731995878
2216	4	0.01918492206675282
2216	5	-0.15888189074610537
2216	6	-0.5338499669918413
2216	7	-1.1476317194763794
2216	8	-6.497113407798825
2216	9	-1.9116836772524017
2216	10	-0.6021692260928759
2216	11	-1.1980299161329313
2216	12	0.02546685188758957
2216	13	0.6331972783153077
2216	14	1.4006519288358348
2216	15	0.8451743154540914
2216	16	0.16766167078710126
2216	17	0.09604856016875019
2216	18	0.24751903891596783
2216	19	0.4438060177165575
2216	20	0.46274492742448103
2216	21	0.2561099043061965
2216	22	0.09233184780110193
2216	23	-0.1971957497725434
2216	24	-0.155959033817048
2216	25	-0.04831738340963554
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	357872.0
3	14.0
4	2680.0
5	16147.0
6	22094.0
7	20112.0
8	18225.0
9	15589.0
10	14213.0
11	13221.0
12	12297.0
13	12121.0
14	12542.0
15	13177.0
16	13667.0
17	14836.0
18	16993.0
19	22012.0
20	29999.0
21	37694.0
22	47503.0
23	57182.0
24	66682.0
25	77252.0
26	87364.0
27	97757.0
28	107346.0
29	126368.0
30	144248.0
31	163540.0
32	206149.0
33	259025.0
34	326961.0
35	407764.0
36	505241.0
37	584223.0
38	745768.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.55244376844785	17.30403948014632	12.729454963801068	24.41406178760476
2	15.016573701315489	19.460286733937004	40.903088100342075	24.62005146440543
3	17.289870236437828	24.193419636373626	29.887098490869857	28.629611636318693
4	11.255295507675616	15.203659506420703	36.8219915443548	36.71905344154888
5	13.764422305182459	37.554841456578785	33.70349259924683	14.977243638991922
6	33.57691630435925	34.984151128252215	17.50185577322955	13.937076794158983
7	30.40252969391682	30.786372393698414	20.128694016772823	18.682403895611944
8	26.7893204352046	34.81859861545309	19.92816657461378	18.46391437472853
9	26.94113788744844	15.735486307861887	19.35886076125847	37.9645150434312
10	13.368403851930807	29.784975305410477	33.73434887385758	23.11227196880114
11	36.112013758273164	22.784373000547163	21.680862714766572	19.4227505264131
12	22.872454972172104	25.27909402972141	30.28845865109986	21.559992347006624
13	28.25795689079601	20.99604270727637	26.454623036286513	24.29137736564111
14	20.964685867097238	20.55527648645762	28.56316782653122	29.91686981991392
15	24.114821945642692	28.63535376130835	23.23278055794276	24.017043735106196
16	23.937664294195567	28.24249394299206	24.618262651082027	23.201579111730346
17	23.006869229843716	28.00033114024411	25.91084477865398	23.081954851258192
18	22.657791519252747	27.30435412970595	27.28350742760275	22.754346923438558
19	23.82714460666036	26.470227409699927	27.18078888838844	22.52183909525127
20	23.858488161645784	26.26591459124595	27.3231553300208	22.552441917087467
21	24.40597024912928	25.694222372069692	26.85228735797109	23.047520020829943
22	24.01638294709881	26.008850789366612	26.692917759896222	23.281848503638354
23	23.68753141980636	26.080776997870746	26.587332906681677	23.64435867564122
24	23.30769674574266	26.323411354093544	26.9704708462905	23.398421053873296
25	23.48337551808754	25.981111867448508	27.001609754779405	23.533902859684545
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13493.0
1	13493.0
2	7970.0
3	2447.0
4	2447.0
5	2447.0
6	4443.5
7	6440.0
8	6440.0
9	6440.0
10	8269.0
11	10098.0
12	10098.0
13	10098.0
14	15035.0
15	19972.0
16	19972.0
17	19972.0
18	32821.0
19	45670.0
20	45670.0
21	45670.0
22	68970.0
23	92270.0
24	92270.0
25	92270.0
26	131751.0
27	171232.0
28	171232.0
29	171232.0
30	217997.5
31	264763.0
32	264763.0
33	264763.0
34	313877.5
35	362992.0
36	362992.0
37	362992.0
38	414028.0
39	465064.0
40	465064.0
41	465064.0
42	509399.5
43	553735.0
44	553735.0
45	553735.0
46	585178.0
47	616621.0
48	616621.0
49	616621.0
50	612773.0
51	608925.0
52	608925.0
53	608925.0
54	565791.0
55	522657.0
56	522657.0
57	522657.0
58	458322.5
59	393988.0
60	393988.0
61	393988.0
62	325266.0
63	256544.0
64	256544.0
65	256544.0
66	200330.5
67	144117.0
68	144117.0
69	144117.0
70	107533.0
71	70949.0
72	70949.0
73	70949.0
74	50607.5
75	30266.0
76	30266.0
77	30266.0
78	22476.5
79	14687.0
80	14687.0
81	14687.0
82	10115.5
83	5544.0
84	5544.0
85	5544.0
86	3978.0
87	2412.0
88	2412.0
89	2412.0
90	1593.0
91	774.0
92	774.0
93	774.0
94	463.5
95	153.0
96	153.0
97	153.0
98	109.0
99	65.0
100	65.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3230409347720364
2	0.32466629796585794
3	0.3359796812491686
4	0.3378189080211246
5	0.3439354063557689
6	0.2865985810579318
7	0.29538837411925634
8	0.2930572611175912
9	0.2925012158144417
10	0.4017427315255017
11	0.2922445795206804
12	0.29192378415347875
13	0.28544371773600596
14	0.2898279210877615
15	0.28411776355157253
16	0.28892969405959695
17	0.2823213094952435
18	0.28379696818437095
19	0.2858928312500882
20	0.47999541476488483
21	0.28570035402976723
22	0.2880528533892458
23	0.2825993321468182
24	0.2865985810579318
25	0.27922028761229445
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4675878.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.76499109250898
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.90957661803012	52.444392434813395
2	7.519293504098898	9.288581926052581
3	2.9030081645552186	5.379128202757018
4	1.408284854857517	3.4793080606395943
5	0.8016718986369452	2.475762883921284
6	0.5416624375110209	2.007346537680895
7	0.3610355262249294	1.5609549242953442
8	0.2469784297675228	1.220369641170632
9	0.18811963286133376	1.0457286703205706
>10	0.9996826314679401	11.256351352816738
>50	0.07716147694574844	3.3022947804701794
>100	0.04066865434043759	4.455588788991941
>500	0.0021247128792480363	0.8847460218075778
>1k	6.617951732791638E-4	0.7567396392964447
>5k	3.4831324909344064E-5	0.17789566673301413
>10k+	3.4831324909344064E-5	0.26481046823275745
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNN	12309	0.26324467832565346	No Hit
GTATCAACGCAGAGTACTTTTTTTT	8269	0.17684379275934914	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4963	0.10614049382811101	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.138635781344167E-5	0.0	0.0	0.0
3	0.0	2.138635781344167E-5	0.0	0.0	0.0
4	0.0	2.138635781344167E-5	0.0	0.0	0.0
5	0.0	2.138635781344167E-5	0.0	0.0	0.0
6	0.0	2.138635781344167E-5	0.0	0.0	0.0
7	0.0	2.138635781344167E-5	0.0	2.138635781344167E-5	0.0
8	0.0	2.138635781344167E-5	0.0	2.138635781344167E-5	0.0
9	0.0	2.138635781344167E-5	0.0	2.138635781344167E-5	0.0
10	4.277271562688334E-5	2.138635781344167E-5	0.0	2.138635781344167E-5	0.0
11	4.277271562688334E-5	2.138635781344167E-5	0.0	2.138635781344167E-5	0.0
12	4.277271562688334E-5	2.138635781344167E-5	0.0	2.138635781344167E-5	0.0
13	4.277271562688334E-5	2.138635781344167E-5	0.0	2.138635781344167E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTCGCG	165	0.0	12.101208	16
TTCGCGC	165	0.0	12.100428	17
GGTATCA	3365	0.0	11.905288	1
TTTAGCG	80	2.8900304E-5	11.866625	2
TCGCGCA	165	7.2759576E-12	11.524093	18
CGCGCCA	220	0.0	11.226582	10
TACCGTA	95	1.3611718E-5	11.000385	7
GTATCAA	7765	0.0	10.416232	1
GCGCGGT	110	6.14043E-6	10.351108	11
TCGCGGT	185	5.820766E-11	10.278355	17
GTATTAG	555	0.0	10.262917	1
CTTCGCG	195	1.4551915E-11	10.239043	15
TGCCGTA	140	9.590258E-8	10.186803	15
GTGGTAT	1345	0.0	10.163721	1
CGCCAGT	225	0.0	10.1213	12
GGACCGA	245	0.0	10.082094	6
TAGCGCG	85	6.5707404E-4	10.06339	4
TGGTATC	1240	0.0	9.876094	2
ACCGAGT	270	0.0	9.851985	8
CGCAGAT	890	0.0	9.820358	8
>>END_MODULE