FastQCFastQC Report
Thu 26 May 2016
SRR1364283_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364283_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4675878
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT164180.3511212225810853No Hit
GGTATCAACGCAGAGTACTTTTTTT119020.25454043069558274No Hit
TATCAACGCAGAGTACTTTTTTTTT116670.24951463660942394No Hit
ACGCAGAGTACTTTTTTTTTTTTTT60180.12870310132129195No Hit
GTGGTATCAACGCAGAGTACATGGG58720.12558069308052947No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGA350.002169330416.28767610
ACCGAGT3300.012.6692788
GGACCGA3150.012.6692776
GGTATCA47650.012.161891
TAACGCT550.003069228312.09133154
AGGACCG4000.011.4036925
CGCAGTT1501.7826096E-1011.39649612
TGGTATC17100.011.16550352
CGCGCCA1752.0008883E-1110.85845110
TCGCGGT1752.0008883E-1110.85450317
GTGGTAT19300.010.8292771
CGAGTGG3950.010.82408810
GTTCGCG1158.8542583E-710.73674916
ATACCGT1106.0375896E-610.3657736
GAACCGC2300.010.3282166
CCAATAC4950.09.9796123
TTCGCGC1151.0305268E-59.91063217
TTGCGCG3150.09.6482418
TAGACAG7650.09.5651885
TGGACCG2303.6379788E-129.5030775
Produced by FastQC (version 0.11.5)