FastQCFastQC Report
Thu 26 May 2016
SRR1364282_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364282_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1153931
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT108010.9360178381549678No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA82100.7114810157626409No Hit
TATCAACGCAGAGTACTTTTTTTTT65640.5688381714331273No Hit
GTACTTTTTTTTTTTTTTTTTTTTT53660.4650191389259843No Hit
GGTATCAACGCAGAGTACTTTTTTT47260.409556550608312No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN32350.2803460518869846No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA29050.2517481547856848No Hit
GAGTACTTTTTTTTTTTTTTTTTTT27220.23588932093860032No Hit
GAACAAAAAAAAAAAAAAAAAAAAA23920.20729142383730048No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22850.19801877235293966No Hit
GTGGTATCAACGCAGAGTACATGGG15170.1314636663717328No Hit
AAACAAAAAAAAAAAAAAAAAAAAA14470.1253974457744874No Hit
GTATCAACGCAGATTACTTTTTTTT12460.1079787266309684No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGAGT250.006030187519.0005238
AAACGCC504.5345296E-617.11014619
CGCATAT907.2759576E-1216.8893538
CGAGGTT402.7469598E-416.6355864
GGACCGA508.7022956E-515.201746
TAACGCA1600.014.8532024
TGGTTCG456.7301013E-414.78654517
TATCACG653.387615E-614.6081612
CTCAACG801.2952114E-714.2429573
TATAACG1550.014.08980752
ATAACGC1750.013.5647213
GCCCCCG655.4198325E-513.16165118
GTACTAG655.4754193E-513.1473451
GTCAACG655.4754193E-513.1473453
TTAGCCT1155.2677933E-912.3991954
ACCGAGT853.93958E-612.2944568
GGTATCA25300.012.2350571
CAACTCA6150.012.204656
GTACAAC1251.4188117E-912.153991
CCGAGGT550.0030789912.0849333
Produced by FastQC (version 0.11.5)