FastQCFastQC Report
Thu 26 May 2016
SRR1364282_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364282_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1153931
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT242922.105151867832652No Hit
TATCAACGCAGAGTACTTTTTTTTT181461.5725376993945044No Hit
GTACTTTTTTTTTTTTTTTTTTTTT180031.5601452773172746No Hit
GGTATCAACGCAGAGTACTTTTTTT166051.438994185960859No Hit
GAGTACTTTTTTTTTTTTTTTTTTT90810.7869621320512231No Hit
ACGCAGAGTACTTTTTTTTTTTTTT76750.6651177583408368No Hit
GTGGTATCAACGCAGAGTACTTTTT34330.29750479014776443No Hit
ATCAACGCAGAGTACTTTTTTTTTT29140.25252809743390203No Hit
GCAGAGTACTTTTTTTTTTTTTTTT26490.22956311945861582No Hit
GTGGTATCAACGCAGAGTACATGGG23220.2012252032400551No Hit
GTATAACGCAGAGTACTTTTTTTTT22930.19871205470691056No Hit
GGTATCAACGCAGAGTACATGGGAT14610.12661068989393648No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA14200.12305761782983558No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT13010.11274504281451837No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTCCG307.7168155E-419.0015610
TAACGCA3050.016.5124174
TATAACG3150.016.285642
ATAACGC3150.015.6845093
TATCACG1200.015.0415972
ACCGAGT602.5640917E-514.2517888
CGGTGAG400.005288806314.24561315
AACGCCG1301.8189894E-1213.8863586
AGGACCG551.9523436E-413.8241115
GTATAAC4650.013.4879031
GGTATCA38500.013.4027071
TGACCGA500.001491371413.30917119
CTTGGAC655.443696E-513.1554983
CGTTCAA751.4824876E-512.66276614
ACAACCG701.086071E-412.2195275
CGAGAGT550.003061754912.0944
CTGGACG550.003061754912.0944
ACCTGCG550.003064739312.0924267
CAACGCC1501.4551915E-1112.0384975
TGCACGG951.0428612E-611.996304512
Produced by FastQC (version 0.11.5)