FastQCFastQC Report
Thu 26 May 2016
SRR1364281_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364281_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1018707
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48130.47246165973140464No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA37200.36516878749238No Hit
TATCAACGCAGAGTACTTTTTTTTT30540.29979179489293784No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN27250.26749595320342356No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22960.2253837462587378No Hit
GGTATCAACGCAGAGTACTTTTTTT22690.22273332763984147No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA16800.1649149362868813No Hit
GAGTACTTTTTTTTTTTTTTTTTTT14550.14282811446274543No Hit
ACGCAGAGTACTTTTTTTTTTTTTT14450.14184647793722827No Hit
GAACAAAAAAAAAAAAAAAAAAAAA13790.13536767686881507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC851.7285856E-814.5202173
TACTAGC400.00529964114.2409822
TAACGCA954.8730726E-914.00425154
ACCGTGC500.00150050513.2981378
TATAACG905.440361E-712.6586512
CTTACAC1600.012.4608593
GGTATCA10800.012.394931
CGCGCCA701.0921095E-412.21197210
CCCTATA701.0966416E-412.2065562
GTCCTAT701.0966416E-412.2065561
CCTATAC701.0966416E-412.2065563
GTCATAC550.00308175812.0832591
TGACCGA1209.855285E-911.88622819
GTATAAG1459.822543E-1111.7856411
GTATCAA28250.011.7288561
TAAGCCT752.0666228E-411.4034624
AACAGAT752.07516E-411.3984027
GTCTTAT752.0846823E-411.3927851
GCGCCAG1001.9546478E-611.38661511
TTGACCG1354.647518E-911.26990618
Produced by FastQC (version 0.11.5)