FastQCFastQC Report
Thu 26 May 2016
SRR1364281_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364281_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1018707
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT126181.2386289678975406No Hit
TATCAACGCAGAGTACTTTTTTTTT98070.9626909405746696No Hit
GGTATCAACGCAGAGTACTTTTTTT96490.9471810834714987No Hit
GTACTTTTTTTTTTTTTTTTTTTTT71230.6992196971258664No Hit
ACGCAGAGTACTTTTTTTTTTTTTT58820.577398604309188No Hit
GAGTACTTTTTTTTTTTTTTTTTTT51830.5087822111755392No Hit
GCAGAGTACTTTTTTTTTTTTTTTT17040.16727086394812246No Hit
GTGGTATCAACGCAGAGTACTTTTT16650.16344248149860557No Hit
ATCAACGCAGAGTACTTTTTTTTTT14550.14282811446274543No Hit
GTGGTATCAACGCAGAGTACATGGG13750.13497502225860822No Hit
TGCATGCACTGCCTCAGTGACCAGT11130.10925614529005886No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT402.7593455E-416.6254736
ATAACGC1550.014.7137093
TTCGCGG653.3769647E-614.61221216
AAGGCCG400.005262714314.2560055
CTTCGCG602.5718038E-514.24690715
TCGCGGT602.5718038E-514.24690717
ACGGCCC903.6441634E-813.71924315
GGTACAT1600.013.6593221
GGTATCA20500.013.3493441
CGGCCCC1102.750312E-912.95173416
TATAACG1750.012.4866822
GTGGTAT5950.012.4565821
TAACGCA1850.012.3289144
GTTATGG550.003062033312.0936291
CCGTCCA550.003066542612.0912529
GACCTAC951.0357744E-612.0026991
CGCGGTT802.8719007E-511.8718418
GCGCCGA657.9843646E-411.69838519
TAGAGGT1157.025483E-811.5695244
TTACACC1406.7484507E-1011.5400094
Produced by FastQC (version 0.11.5)