##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364281_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1018707
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.311063927115452	31.0	30.0	34.0	25.0	34.0
2	29.326860422084074	31.0	30.0	34.0	25.0	34.0
3	29.357034947241946	31.0	29.0	34.0	25.0	34.0
4	32.67742932953244	35.0	32.0	37.0	26.0	37.0
5	32.26717986624221	35.0	32.0	37.0	23.0	37.0
6	32.14543239616494	35.0	32.0	37.0	22.0	37.0
7	31.865774948046887	35.0	32.0	37.0	18.0	37.0
8	31.74182272233331	35.0	32.0	37.0	17.0	37.0
9	32.457528023268715	37.0	32.0	39.0	11.0	39.0
10	32.57125257802293	37.0	32.0	39.0	16.0	39.0
11	32.50844060166466	37.0	32.0	39.0	11.0	39.0
12	32.44543622454739	37.0	32.0	39.0	11.0	39.0
13	32.47603285341123	37.0	32.0	39.0	11.0	39.0
14	33.37036753453152	38.0	32.0	41.0	10.0	41.0
15	33.395401229205255	38.0	32.0	41.0	10.0	41.0
16	33.340563086343764	38.0	32.0	41.0	10.0	41.0
17	33.335917000668495	38.0	32.0	41.0	10.0	41.0
18	33.28660743471872	38.0	32.0	41.0	10.0	41.0
19	33.20621434818844	38.0	32.0	41.0	8.0	41.0
20	33.099670464618384	38.0	32.0	41.0	2.0	41.0
21	33.022737646840554	38.0	32.0	41.0	2.0	41.0
22	32.966350481541795	38.0	32.0	41.0	2.0	41.0
23	32.68094947811294	38.0	32.0	41.0	2.0	41.0
24	32.588272192102345	38.0	32.0	41.0	2.0	41.0
25	32.41777567053137	38.0	32.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.495900413087039
1101	2	0.8684079262396622
1101	3	-1.2758745877573219
1101	4	-0.045635399291526824
1101	5	0.745575859908385
1101	6	0.9628596252470984
1101	7	1.3850115247320076
1101	8	1.2459588347302635
1101	9	2.1563958178669687
1101	10	1.600520107328073
1101	11	1.6032205733138625
1101	12	1.8091935968723547
1101	13	1.4437991252326015
1101	14	1.7245045070035303
1101	15	1.710942753461694
1101	16	1.6929082208041422
1101	17	1.5478153543762758
1101	18	1.4677999351276583
1101	19	1.659459176777169
1101	20	1.933346076108755
1101	21	1.7282965157669636
1101	22	1.8359848318812269
1101	23	2.2555488570613065
1101	24	2.2985707292254034
1101	25	2.2978230480091675
1102	1	0.9978193252143832
1102	2	0.9973314210428263
1102	3	1.1388527067983034
1102	4	1.062839648721834
1102	5	1.4193845931307436
1102	6	1.372840564113389
1102	7	1.4522370824423163
1102	8	1.6987842413070915
1102	9	2.252438766887895
1102	10	1.8264116668907775
1102	11	1.8341112189372168
1102	12	2.025598934566645
1102	13	1.888580423329337
1102	14	1.8554415526251518
1102	15	1.9384894986658594
1102	16	1.987338742845587
1102	17	1.9129090719174542
1102	18	2.1093352523042768
1102	19	2.0744565070647596
1102	20	2.206930128065217
1102	21	2.3909912416338273
1102	22	2.5209529990308255
1102	23	2.628324712622117
1102	24	2.7136322335587764
1102	25	2.793615141406203
1103	1	-3.4789281691623906
1103	2	-3.2525652404524408
1103	3	-2.9727153006742775
1103	4	-2.757088309577721
1103	5	-6.004609265609545
1103	6	-9.14012103690872
1103	7	-12.250486222671999
1103	8	-11.601729498687863
1103	9	-13.520372201487838
1103	10	-9.885045754961155
1103	11	-12.302311712011193
1103	12	-12.86232307572963
1103	13	-11.597239386527814
1103	14	-12.942224339226406
1103	15	-11.89033362339168
1103	16	-6.506066381670603
1103	17	-4.890522303263051
1103	18	-4.482185085250279
1103	19	-3.9241638567941415
1103	20	-9.13946053979678
1103	21	-5.492480502666449
1103	22	-10.738203866135244
1103	23	-13.575040096456643
1103	24	-16.656083411085607
1103	25	-17.4558950072402
1104	1	0.5164551149940486
1104	2	0.7468274383713975
1104	3	0.7502871232359034
1104	4	0.8802119596859157
1104	5	0.9331161284774012
1104	6	0.9367239027085077
1104	7	0.7878986254708309
1104	8	1.083807120629249
1104	9	1.6047789569907458
1104	10	1.5985861331627618
1104	11	1.246561519098698
1104	12	1.3988196554958137
1104	13	1.1364260923691205
1104	14	1.2032908894864462
1104	15	1.2009204142192331
1104	16	0.8726010589274722
1104	17	0.7863774564957424
1104	18	1.0679296608303517
1104	19	1.1090399505184365
1104	20	1.2688635224832012
1104	21	1.168370929375385
1104	22	0.9065239948580768
1104	23	1.3919925752728233
1104	24	1.6523981247382835
1104	25	1.6104749857191756
1105	1	0.5756633143065386
1105	2	0.5996504424279436
1105	3	0.11920091881680506
1105	4	0.47920431635269267
1105	5	0.5916182313612026
1105	6	0.5535997524283047
1105	7	0.7451808623202112
1105	8	0.4632425889008509
1105	9	0.5748978348632114
1105	10	0.15168394903746218
1105	11	0.8042422952590158
1105	12	1.007067395102979
1105	13	0.8658632186305724
1105	14	0.7048297113188582
1105	15	0.4902596776296093
1105	16	0.7785698914815526
1105	17	0.7089400041629474
1105	18	0.6972387073756536
1105	19	0.9035960099186866
1105	20	1.0307402564642558
1105	21	0.9039136032708868
1105	22	0.8730184378287547
1105	23	1.3071435628821106
1105	24	1.373170526944513
1105	25	1.5188323599053781
1106	1	0.25489020477749946
1106	2	0.5391333005732974
1106	3	0.3692603408946553
1106	4	-0.8619682510206275
1106	5	0.1786733439235917
1106	6	0.3815544445087582
1106	7	0.808241481328487
1106	8	0.7083314249072323
1106	9	1.4158093695374099
1106	10	0.8990799886974514
1106	11	1.0309354000342879
1106	12	1.099889986245131
1106	13	0.7239275727408838
1106	14	0.32356103580868023
1106	15	0.9657006743359275
1106	16	0.5092333106845075
1106	17	0.4853272974590084
1106	18	0.4829007966559047
1106	19	-0.7793451141206589
1106	20	0.4253622754566848
1106	21	0.1688734650721102
1106	22	0.6202712278367883
1106	23	0.5054704069472606
1106	24	0.6260142978129792
1106	25	1.04794917805118
1107	1	0.04144591604590531
1107	2	-0.10000271232103586
1107	3	-0.3470116681802118
1107	4	-0.23795895233524078
1107	5	0.21844709722865474
1107	6	-0.07239449919735108
1107	7	-0.14058341147611486
1107	8	-0.40249522865968856
1107	9	0.4153075922429572
1107	10	0.4884059757108332
1107	11	0.15908227217409632
1107	12	0.12889714665435292
1107	13	0.27144181307875925
1107	14	0.25434265658930855
1107	15	0.08756134157255957
1107	16	0.02194833279213526
1107	17	-0.13051585735037463
1107	18	-0.2611680758203505
1107	19	0.0429508748947427
1107	20	-0.07478469696591361
1107	21	-0.2670180362157595
1107	22	0.03695603139696857
1107	23	0.11218310438354706
1107	24	0.4469622826557469
1107	25	1.6442852016851361E-4
1108	1	-0.21132269246068347
1108	2	-0.20481420171955733
1108	3	-0.05264056767742886
1108	4	-0.25622107218891443
1108	5	0.17565899238660876
1108	6	-0.03471962590512234
1108	7	-0.22364369178886534
1108	8	0.16838287966187693
1108	9	0.42887070863736554
1108	10	0.21076306241705822
1108	11	0.2892389976734222
1108	12	0.36651605312901836
1108	13	-0.14891390759526857
1108	14	-0.08989451016419281
1108	15	-0.3070871997002058
1108	16	-0.818394537606018
1108	17	-0.7963085334295315
1108	18	-0.9458252514475447
1108	19	-0.8464638484285061
1108	20	-0.275106506166928
1108	21	-0.6992287807401247
1108	22	-0.6149557693169747
1108	23	-0.8612842056560517
1108	24	-0.50025953556624
1108	25	-0.7813683626959858
1109	1	0.24962467134572464
1109	2	0.23096620803026724
1109	3	0.19183789269198925
1109	4	0.3400193562656071
1109	5	0.36057686706512015
1109	6	0.308146650106103
1109	7	0.539028376833997
1109	8	0.4068769321519419
1109	9	1.2446297833392599
1109	10	0.5237018191390561
1109	11	0.3967223388003802
1109	12	0.5382040061334621
1109	13	0.4893211286450878
1109	14	0.1998065799690778
1109	15	0.054063650779106354
1109	16	0.254894274645558
1109	17	0.24598418050547366
1109	18	0.23927199837709878
1109	19	0.47996348089110796
1109	20	0.6995424153902334
1109	21	0.5362498695988975
1109	22	0.42949748354718054
1109	23	0.6869625940867579
1109	24	0.39401141954247976
1109	25	0.39580532797504375
1110	1	0.24467722054071572
1110	2	0.36419798352221733
1110	3	0.1761197203604432
1110	4	0.3347970470825459
1110	5	0.25065215030900845
1110	6	0.27074261760758844
1110	7	0.22837784761515678
1110	8	0.13214433674347248
1110	9	-3.32113590688137
1110	10	-1.283529285512106
1110	11	-0.25304286692412603
1110	12	0.3281656191328608
1110	13	-0.03141903235670185
1110	14	0.1538679619010992
1110	15	0.3007513743926964
1110	16	-0.14546723400083295
1110	17	0.19804235730929065
1110	18	0.1952806820384012
1110	19	-0.17387801433847017
1110	20	0.2889366088905376
1110	21	0.1273025306006872
1110	22	0.37826667704748473
1110	23	0.39057674882736393
1110	24	0.5368725554995493
1110	25	0.5843581753214941
1111	1	-0.05061949885415373
1111	2	0.05808329615867436
1111	3	0.007924734000287259
1111	4	-0.1670928106029521
1111	5	-0.14860317533342737
1111	6	-0.30074095589026584
1111	7	-0.09333931698792597
1111	8	0.00271373560439514
1111	9	0.5927757933332067
1111	10	0.35030785336073933
1111	11	0.42922158168348545
1111	12	0.484439242353929
1111	13	0.10305901477470059
1111	14	0.05809795640760029
1111	15	0.14691930644233508
1111	16	0.14550981068432378
1111	17	0.16687966051758707
1111	18	0.06832919855880704
1111	19	0.015694666536170132
1111	20	0.2201880468257329
1111	21	0.2962769365280309
1111	22	0.34646541964652044
1111	23	0.5482824381206797
1111	24	0.4851209743577414
1111	25	0.7378991347588091
1112	1	0.14744913017834804
1112	2	-0.01849926839029692
1112	3	0.025181292382750797
1112	4	0.14914008007697532
1112	5	0.19700661194335822
1112	6	0.02743117368553527
1112	7	0.2113412633821241
1112	8	-0.3723889887989671
1112	9	0.651945810590906
1112	10	0.21017807058861138
1112	11	0.2135589255804362
1112	12	-0.22494466993921236
1112	13	-0.3059895594105768
1112	14	-1.0685151971859312
1112	15	-0.42129692614397385
1112	16	-0.47475155063468577
1112	17	-0.4629601807237478
1112	18	-0.4077763442978082
1112	19	-0.03673358617498934
1112	20	-0.025709444872617837
1112	21	-0.395913954328428
1112	22	0.021535196148654734
1112	23	0.0595376085823105
1112	24	0.17041144853359214
1112	25	0.07044656299630603
1113	1	-2.1402833920820754
1113	2	-2.290501825033161
1113	3	-2.3484918981312255
1113	4	-2.197761092452339
1113	5	-2.1452176673478363
1113	6	-1.864746821553819
1113	7	-2.399802173098525
1113	8	-2.4445590496294365
1113	9	-11.44886085431256
1113	10	-4.666600559403452
1113	11	-3.530816460119997
1113	12	-12.842847781990695
1113	13	-5.242434944229409
1113	14	-4.614669212878429
1113	15	-4.097174119126429
1113	16	-3.8910655769063993
1113	17	-3.8186729551220573
1113	18	-3.7492380387349087
1113	19	-4.242512480647353
1113	20	-15.0409413059323
1113	21	-17.19204592999624
1113	22	-12.027049834266581
1113	23	-18.4481609720401
1113	24	-19.075687467097197
1113	25	-15.148893558664632
1114	1	-0.5660413152035062
1114	2	-0.44145216291894585
1114	3	-0.46373223918298123
1114	4	-0.9626853820188188
1114	5	-0.7156955109924183
1114	6	-0.6277868110733564
1114	7	-0.6114300292978285
1114	8	-0.45060169201636313
1114	9	0.004573135141473728
1114	10	-0.8274822284173062
1114	11	-0.9607327255814084
1114	12	-0.6561208526574056
1114	13	-0.8035786248372752
1114	14	-0.7358965188533801
1114	15	-0.5018040823356387
1114	16	-0.8650063404518491
1114	17	-0.7140124238935499
1114	18	-0.9066240363986395
1114	19	-1.1310625739889808
1114	20	-1.6053066374315534
1114	21	-1.6175069066444578
1114	22	-1.751819755335589
1114	23	-1.2966080074880821
1114	24	-0.7473869397988615
1114	25	-1.2130012454297692
1115	1	-0.39140389406696485
1115	2	-0.23462399176385418
1115	3	-0.39195456940795737
1115	4	-0.17756644525039178
1115	5	-0.37955625103332835
1115	6	-0.35231885382427564
1115	7	-0.6608023011558934
1115	8	-0.3543746470170639
1115	9	0.13775074768513562
1115	10	-0.38386269283923014
1115	11	-0.3199571951692981
1115	12	-0.4215710500147729
1115	13	-0.38309956197815964
1115	14	-0.4634078477868684
1115	15	-0.6381460745624636
1115	16	-0.47820861698835415
1115	17	-0.2699727374245313
1115	18	-0.45488536873875063
1115	19	-0.5811986120342141
1115	20	-0.9464093556185595
1115	21	-0.5070734623307303
1115	22	-0.942237438313196
1115	23	-0.6749697823871337
1115	24	-0.2925206083444749
1115	25	-0.4084274298389161
1116	1	-2.4833134339215768
1116	2	-2.4830874343994616
1116	3	-2.5489785292432927
1116	4	-2.383260921862096
1116	5	-4.01568041582599
1116	6	-3.835058732344102
1116	7	-5.357817220953574
1116	8	-4.208417262202836
1116	9	-2.9535368226366465
1116	10	-4.539746063901646
1116	11	-3.344727645452828
1116	12	-4.496280237233204
1116	13	-4.413818880015651
1116	14	-4.019637878144458
1116	15	-5.650186383667002
1116	16	-5.985788053517577
1116	17	-6.756635568923706
1116	18	-5.591422312110261
1116	19	-5.056125581977955
1116	20	-5.4700080875229204
1116	21	-4.683432020606595
1116	22	-4.7573412742277945
1116	23	-4.767109058735855
1116	24	-5.094703789889849
1116	25	-4.789813668560662
1201	1	1.011384721390364
1201	2	0.9790230868188594
1201	3	0.5828769944729189
1201	4	0.9120536092377449
1201	5	1.2011990770176624
1201	6	1.284727505681495
1201	7	1.4541143837180712
1201	8	1.3117506311087546
1201	9	2.1033913400095336
1201	10	1.700978130755054
1201	11	1.7839559626902357
1201	12	1.9132957840104368
1201	13	1.6012328691647966
1201	14	1.6086715152399762
1201	15	1.7246254929152798
1201	16	1.784614153880554
1201	17	1.5197381306252993
1201	18	1.6252445942372589
1201	19	1.6561435975444638
1201	20	1.8538030381288308
1201	21	1.8604699044891788
1201	22	1.8044648106229815
1201	23	2.1151405315661975
1201	24	2.113779786865443
1201	25	2.3401460838135897
1202	1	1.3181371399601964
1202	2	1.4125696518787052
1202	3	1.4003497906604885
1202	4	1.576225059872094
1202	5	1.6082728839562392
1202	6	1.6610223491474159
1202	7	1.7582851817872083
1202	8	1.8458516953338595
1202	9	2.3968520927435115
1202	10	2.147611528680258
1202	11	2.300955105292047
1202	12	2.4659866185507155
1202	13	2.073073411228968
1202	14	2.410542927264217
1202	15	2.3904983260815484
1202	16	2.355714578229218
1202	17	2.282123546517802
1202	18	2.441589177674068
1202	19	2.2622352938333634
1202	20	2.549745351363761
1202	21	2.662116181513987
1202	22	2.6776510871764927
1202	23	2.913174860121913
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2211	13	0.39071866155455126
2211	14	0.21690564977296134
2211	15	0.06604300400736207
2211	16	0.20116733365872363
2211	17	0.19052991234929806
2211	18	0.24819267425245073
2211	19	-0.17359513489648037
2211	20	0.31651023911191345
2211	21	0.4736792093947244
2211	22	0.42272040040041503
2211	23	0.8017154652697087
2211	24	0.7848602934566955
2211	25	0.9110721297123057
2212	1	0.06323756308361439
2212	2	0.1537537792553394
2212	3	0.053305129159223696
2212	4	0.2058268705106201
2212	5	0.22671824883150293
2212	6	0.321790702812649
2212	7	0.34647584834397094
2212	8	0.36184933243684014
2212	9	0.7679337363307148
2212	10	0.4699491481988858
2212	11	0.3506041051821498
2212	12	0.3497691770736324
2212	13	0.22903800898696858
2212	14	0.2061945785445758
2212	15	0.3425919627449119
2212	16	0.3094704854717705
2212	17	0.11481876027755078
2212	18	-0.0359461284035234
2212	19	0.19001066750079332
2212	20	0.19400021453553506
2212	21	0.43851571717724624
2212	22	0.162381208618406
2212	23	0.5328270883887498
2212	24	0.47752281555387555
2212	25	0.4764232054679205
2213	1	-0.29592537408128905
2213	2	-0.2992758800981399
2213	3	-0.47057314607239675
2213	4	-0.3845367839524414
2213	5	-0.3412137144006593
2213	6	-0.12993538350989198
2213	7	-0.10787596144583134
2213	8	-0.22381564053128145
2213	9	0.5346292155015853
2213	10	-0.08215731926190983
2213	11	0.0688339378312719
2213	12	0.053791150288518
2213	13	-0.3457598828630921
2213	14	-0.20477303087283616
2213	15	-0.19711461239472783
2213	16	-0.292406603610047
2213	17	-0.41034626316503164
2213	18	-0.3792783120408245
2213	19	-0.43796977925426006
2213	20	-0.11112630811066992
2213	21	-0.4699253651073363
2213	22	-0.21016692438740137
2213	23	-0.4638314322609105
2213	24	-0.35013038845267275
2213	25	-0.17124683737928592
2214	1	-1.1031629617727923
2214	2	-1.0127303017507998
2214	3	-1.0724716335660673
2214	4	-1.0100605576929809
2214	5	-1.2727030474647947
2214	6	-0.9318919571200084
2214	7	-0.8682232257301017
2214	8	-0.7539533017481475
2214	9	-10.861773260104222
2214	10	-4.113384185134446
2214	11	-1.9497957264676842
2214	12	-1.2963612447920845
2214	13	-1.1263077277925966
2214	14	-1.171816537518012
2214	15	-1.2720842107547874
2214	16	-1.4916534994757455
2214	17	-1.2622918061712483
2214	18	-1.6338801252345796
2214	19	-1.8117935263989864
2214	20	-1.4541726909903119
2214	21	-2.1723880508956377
2214	22	-2.0392335913419792
2214	23	-2.3368841168003947
2214	24	-2.409865157312037
2214	25	-10.098282181737464
2215	1	-0.6787275732746068
2215	2	-0.32677981905358067
2215	3	-0.22942493812671216
2215	4	-0.4647493025273661
2215	5	-0.5173618541814804
2215	6	-0.5273123994857869
2215	7	-0.36833040686622454
2215	8	-0.44268055919112115
2215	9	0.4088646491567225
2215	10	0.37936322465476735
2215	11	0.4746610600469836
2215	12	0.41965574370456693
2215	13	0.3352600972327693
2215	14	0.07342803989982372
2215	15	-0.33439982003464763
2215	16	-0.06990012307890225
2215	17	-0.02547613670946447
2215	18	-0.004690173895959049
2215	19	-0.256338847206834
2215	20	0.17507381224900342
2215	21	-0.10932465736127739
2215	22	-0.23849001026928818
2215	23	-0.011713744646854707
2215	24	0.3717994173164101
2215	25	0.4420016858775959
2216	1	-1.9557636231843354
2216	2	-1.9516036337251386
2216	3	-1.9022789883848041
2216	4	-1.7578929353872788
2216	5	-3.1657000857756756
2216	6	-4.173300018820417
2216	7	-3.1634367539616868
2216	8	-4.059687800138555
2216	9	-2.581263442899246
2216	10	-3.2322339032962866
2216	11	-2.4080411210845334
2216	12	-4.004528854836181
2216	13	-3.60943498948442
2216	14	-3.7098058075896247
2216	15	-3.5131928848714047
2216	16	-4.427986124190344
2216	17	-4.78023334793556
2216	18	-4.182548744562247
2216	19	-6.007000872736775
2216	20	-4.323901285416696
2216	21	-4.556140603481332
2216	22	-4.65589537891065
2216	23	-4.118343953138588
2216	24	-4.174388648253007
2216	25	-4.436244782812707
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	42960.0
3	0.0
4	653.0
5	6060.0
6	8030.0
7	6270.0
8	5008.0
9	3959.0
10	3398.0
11	3133.0
12	2709.0
13	2470.0
14	2625.0
15	2617.0
16	2631.0
17	2944.0
18	3330.0
19	4020.0
20	5335.0
21	6991.0
22	8584.0
23	9687.0
24	11227.0
25	12659.0
26	13983.0
27	15328.0
28	17232.0
29	19594.0
30	24079.0
31	30017.0
32	36755.0
33	45118.0
34	56689.0
35	70788.0
36	92774.0
37	137815.0
38	301235.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.8522637450254	18.320481764453472	11.846158226984269	23.981096263536863
2	17.69961195222487	21.62603110468455	35.95228769526322	24.722069247827356
3	19.95906567652068	26.022646399104744	29.041969952046966	24.97631797232761
4	12.536199019509676	16.524619259939882	34.946410726100794	35.99277099444965
5	12.601395703699195	39.284728857013526	33.215077213423655	14.898798225863624
6	31.777975433140128	36.443852787333306	17.10127939305405	14.67689238647252
7	28.161351765306524	33.05816282449044	20.672249931272827	18.10823547893021
8	28.13289187904781	33.845777709700606	19.27326962880921	18.748060782442373
9	27.512368143511708	14.686225256348065	18.950018755977855	38.851387844162375
10	15.653754524618046	30.892019978042224	30.883967483634777	22.57025801370495
11	35.243580301468064	22.80463494869151	21.740364314822997	20.21142043501743
12	23.505354218778837	27.060893194428626	29.102643142398264	20.33110944439427
13	29.163284037754554	21.280965165875614	26.42337916038815	23.132371635981684
14	22.17606081786839	20.802377268693405	28.477157958391906	28.5444039550463
15	24.17388128786265	28.38239668423569	24.579222087294053	22.864499940607608
16	22.62718187351515	28.24272054348039	26.01392079480081	23.11617678820365
17	22.152094457485003	27.704819377646356	27.499344699725214	22.643741465143425
18	21.466713399171187	25.61333957742427	30.613815738447204	22.30613128495734
19	22.7774592465471	25.20774345058337	30.681528112126482	21.333269190743046
20	22.687993214042375	24.663598324716713	30.802671185402335	21.845737275838577
21	23.326147055589587	24.111041735387904	30.842188555361826	21.720622653660683
22	22.989300156192783	24.434304294172566	30.54457384698605	22.031821702648603
23	22.10839842887169	24.383510810097082	31.362583703028868	22.14550705800236
24	22.50386784154808	24.68468256212107	30.747867778720195	22.063581817610654
25	22.042460283147165	24.51813100862333	31.238847444836615	22.200561263392892
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1170.0
1	1170.0
2	1525.0
3	1880.0
4	1880.0
5	1880.0
6	5304.5
7	8729.0
8	8729.0
9	8729.0
10	8635.0
11	8541.0
12	8541.0
13	8541.0
14	7267.5
15	5994.0
16	5994.0
17	5994.0
18	9480.0
19	12966.0
20	12966.0
21	12966.0
22	19897.0
23	26828.0
24	26828.0
25	26828.0
26	37362.0
27	47896.0
28	47896.0
29	47896.0
30	55246.5
31	62597.0
32	62597.0
33	62597.0
34	70551.5
35	78506.0
36	78506.0
37	78506.0
38	83722.0
39	88938.0
40	88938.0
41	88938.0
42	97478.0
43	106018.0
44	106018.0
45	106018.0
46	114973.5
47	123929.0
48	123929.0
49	123929.0
50	123743.5
51	123558.0
52	123558.0
53	123558.0
54	117187.5
55	110817.0
56	110817.0
57	110817.0
58	98188.5
59	85560.0
60	85560.0
61	85560.0
62	71959.5
63	58359.0
64	58359.0
65	58359.0
66	45730.5
67	33102.0
68	33102.0
69	33102.0
70	25403.5
71	17705.0
72	17705.0
73	17705.0
74	12730.5
75	7756.0
76	7756.0
77	7756.0
78	6182.0
79	4608.0
80	4608.0
81	4608.0
82	3296.0
83	1984.0
84	1984.0
85	1984.0
86	1439.0
87	894.0
88	894.0
89	894.0
90	610.0
91	326.0
92	326.0
93	326.0
94	185.5
95	45.0
96	45.0
97	45.0
98	23.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.027878477324687078
2	0.0017669457459308711
3	1.9632730510343014E-4
4	0.0032394005342065973
5	0.02905644115530766
6	0.009718201602619792
7	0.018356603027170716
8	0.025031731400687342
9	0.035829733181376
10	0.03818566084261716
11	0.03504442396096228
12	0.0069696193311717695
13	0.0021596003561377315
14	0.005791655500551189
15	0.005693491847999474
16	0.00854023777199921
17	0.008834728729654356
18	0.014135565967446969
19	0.014037402314895257
20	0.013055765789378105
21	0.009718201602619792
22	0.009227383339861216
23	0.007460437593930345
24	0.005006346280137468
25	0.09845814350937021
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1018707.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.43179427568046
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.11189835945062	35.62692110641282
2	12.565590232493182	11.920169817202328
3	4.796871259787282	6.825726322834635
4	2.324252486935505	4.409738632202542
5	1.3146406861864492	3.1177883286817525
6	0.8060214736629512	2.293862683233714
7	0.5657885964729228	1.8785457818000766
8	0.39291039040086595	1.4909155845016935
9	0.2973080495281213	1.2691668817549497
>10	1.603685130929026	14.071563456526167
>50	0.14819218905314716	4.838889862144229
>100	0.06784632378292596	5.7012207898873655
>500	0.002705528213349811	0.8721372223816766
>1k	0.001040587774365312	0.7217959671822092
>5k	0.001040587774365312	3.7159856931902464
>10k+	2.081175548730624E-4	1.2455718700636098
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	12618	1.2386289678975406	No Hit
TATCAACGCAGAGTACTTTTTTTTT	9807	0.9626909405746696	No Hit
GGTATCAACGCAGAGTACTTTTTTT	9649	0.9471810834714987	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	7123	0.6992196971258664	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	5882	0.577398604309188	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	5183	0.5087822111755392	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	1704	0.16727086394812246	No Hit
GTGGTATCAACGCAGAGTACTTTTT	1665	0.16344248149860557	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	1455	0.14282811446274543	No Hit
GTGGTATCAACGCAGAGTACATGGG	1375	0.13497502225860822	No Hit
TGCATGCACTGCCTCAGTGACCAGT	1113	0.10925614529005886	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	9.816365255171507E-5	0.0	0.0	0.0	0.0
3	9.816365255171507E-5	0.0	0.0	0.0	0.0
4	9.816365255171507E-5	0.0	0.0	0.0	0.0
5	9.816365255171507E-5	0.0	0.0	0.0	0.0
6	9.816365255171507E-5	0.0	0.0	0.0	0.0
7	1.9632730510343014E-4	0.0	0.0	0.0	0.0
8	1.9632730510343014E-4	0.0	0.0	0.0	0.0
9	1.9632730510343014E-4	0.0	0.0	0.0	0.0
10	1.9632730510343014E-4	0.0	0.0	0.0	0.0
11	1.9632730510343014E-4	0.0	0.0	0.0	0.0
12	3.926546102068603E-4	0.0	0.0	0.0	0.0
13	3.926546102068603E-4	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACCGT	40	2.7593455E-4	16.625473	6
ATAACGC	155	0.0	14.713709	3
TTCGCGG	65	3.3769647E-6	14.612212	16
AAGGCCG	40	0.0052627143	14.256005	5
CTTCGCG	60	2.5718038E-5	14.246907	15
TCGCGGT	60	2.5718038E-5	14.246907	17
ACGGCCC	90	3.6441634E-8	13.719243	15
GGTACAT	160	0.0	13.659322	1
GGTATCA	2050	0.0	13.349344	1
CGGCCCC	110	2.750312E-9	12.951734	16
TATAACG	175	0.0	12.486682	2
GTGGTAT	595	0.0	12.456582	1
TAACGCA	185	0.0	12.328914	4
GTTATGG	55	0.0030620333	12.093629	1
CCGTCCA	55	0.0030665426	12.091252	9
GACCTAC	95	1.0357744E-6	12.002699	1
CGCGGTT	80	2.8719007E-5	11.87184	18
GCGCCGA	65	7.9843646E-4	11.698385	19
TAGAGGT	115	7.025483E-8	11.569524	4
TTACACC	140	6.7484507E-10	11.540009	4
>>END_MODULE