FastQCFastQC Report
Thu 26 May 2016
SRR1364280_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364280_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1489109
Sequences flagged as poor quality0
Sequence length25
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT137350.9223636416138778No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA98270.659924827531094No Hit
TATCAACGCAGAGTACTTTTTTTTT87880.5901515604297604No Hit
GTACTTTTTTTTTTTTTTTTTTTTT74780.5021794912259613No Hit
GGTATCAACGCAGAGTACTTTTTTT56960.38251061540827436No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA54870.3684753768864468No Hit
GAACAAAAAAAAAAAAAAAAAAAAA49800.33442817147703763No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN39630.26613229790431725No Hit
GAGTACTTTTTTTTTTTTTTTTTTT38790.2604913407950661No Hit
ACGCAGAGTACTTTTTTTTTTTTTT31770.21334905638203785No Hit
GTATCAACGCAGATTACTTTTTTTT18230.12242220012101196No Hit
GTGGTATCAACGCAGAGTACATGGG16790.11275198793372412No Hit
AAACAAAAAAAAAAAAAAAAAAAAA16660.11187898266681619No Hit
GAAAACAAAAAAAAAAAAAAAAAAA16160.1085212701017857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG907.2759576E-1216.8762472
TGCGCCA402.788268E-416.60360312
TCGACCG350.002156586216.30099318
CGCATAT1400.016.285058
CTCAACG551.1362732E-515.533823
TATAACG2900.015.3850272
ATAACGC3100.015.3111123
CGTGCGC508.719049E-515.19886710
TAACGCA3000.014.8892544
GTCGGTT902.3301254E-914.75875912
GTCGTGC400.00524621314.26336817
AAACCCG400.00526622114.2551875
GCGCCAC400.005323059414.23213913
GAACAAA13300.013.9181451
GAACTCG551.9607345E-413.8176189
CTGGTCG903.6401616E-813.72166259
TCACGCA1351.8189894E-1213.3756894
TGGTCGG1001.0080839E-813.29900810
GTGCGCC500.001513344913.28377811
ATCACGC1153.8198777E-1013.2074993
Produced by FastQC (version 0.11.5)