FastQCFastQC Report
Thu 26 May 2016
SRR1364280_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364280_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1489109
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT328852.2083675540205587No Hit
GTACTTTTTTTTTTTTTTTTTTTTT268031.799935397610249No Hit
TATCAACGCAGAGTACTTTTTTTTT253131.69987556317234No Hit
GGTATCAACGCAGAGTACTTTTTTT217631.4614779710551746No Hit
GAGTACTTTTTTTTTTTTTTTTTTT133250.8948303985806279No Hit
ACGCAGAGTACTTTTTTTTTTTTTT102770.6901442406163686No Hit
GTGGTATCAACGCAGAGTACTTTTT43350.2911136793881442No Hit
ATCAACGCAGAGTACTTTTTTTTTT41810.2807719246878503No Hit
GCAGAGTACTTTTTTTTTTTTTTTT40030.2688184679563417No Hit
GTATAACGCAGAGTACTTTTTTTTT31870.21402059889504393No Hit
GTGGTATCAACGCAGAGTACATGGG27680.18588296760008838No Hit
GGTATCAACGCAGAGTACATGGGAT22720.15257445895498584No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT19280.12947339650757603No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA18050.121213423597601No Hit
GGTATCAACGCAGAGTACATGGGAG16720.11228190817461985No Hit
CAACGCAGAGTACTTTTTTTTTTTT16060.1078497275887796No Hit
GGTATCAACGCAGAGTACATGGGAA15340.10301462149513568No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTACG350.002171859816.28378516
TATCACG1800.015.8330542
ATAACGC4650.015.7309053
TATAACG4450.015.5839942
TAACGCA4550.015.0337114
CGCAGTC400.00528900414.2459212
TGGTACG551.9620347E-413.81654617
AGGACCG707.245757E-613.574385
CGTGCGC500.001500074913.29931810
GTATAAC5750.012.8910591
GTATCAC2450.012.7999921
GGTATCA50850.012.7080761
GGTACGA604.097097E-412.664741518
ATAAGGC1251.4097168E-912.1597853
AGACGAA1201.0017175E-811.87439110
CGAAATC1052.7235546E-711.76051213
TTACCGC658.0310495E-411.69092416
GGCGTGC907.464114E-611.6105178
ATCCACG907.464114E-611.6105176
GTATAAA3450.011.5688991
Produced by FastQC (version 0.11.5)