##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364280_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1489109 Sequences flagged as poor quality 0 Sequence length 25 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.473079539509868 31.0 30.0 34.0 26.0 34.0 2 29.429532693711476 31.0 30.0 34.0 25.0 34.0 3 29.46391164112231 31.0 30.0 34.0 25.0 34.0 4 32.775119215584624 35.0 33.0 37.0 26.0 37.0 5 32.41849052017012 35.0 32.0 37.0 25.0 37.0 6 32.22956747961365 35.0 32.0 37.0 22.0 37.0 7 31.94643239682253 35.0 32.0 37.0 18.0 37.0 8 31.787766375732065 35.0 32.0 37.0 17.0 37.0 9 32.41473794060744 37.0 32.0 39.0 11.0 39.0 10 32.60282222456516 37.0 32.0 39.0 15.0 39.0 11 32.510700694173494 37.0 32.0 39.0 11.0 39.0 12 32.47144030423562 37.0 32.0 39.0 11.0 39.0 13 32.475832863813196 37.0 32.0 39.0 11.0 39.0 14 33.335837067669324 38.0 32.0 41.0 11.0 41.0 15 33.38290212469336 38.0 32.0 41.0 11.0 41.0 16 33.37530227807367 38.0 32.0 41.0 11.0 41.0 17 33.4518292482283 38.0 32.0 41.0 11.0 41.0 18 33.45395333719694 38.0 32.0 41.0 10.0 41.0 19 33.41721190322535 39.0 32.0 41.0 10.0 41.0 20 33.3067095827102 39.0 32.0 41.0 8.0 41.0 21 33.25057668713304 38.0 32.0 41.0 2.0 41.0 22 33.17083974376624 38.0 32.0 41.0 2.0 41.0 23 32.89072660228365 38.0 32.0 41.0 2.0 41.0 24 32.80978356856348 38.0 32.0 41.0 2.0 41.0 25 32.65058635734523 38.0 32.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.060527772236643074 1101 2 0.3896474309684841 1101 3 -1.3449536760808734 1101 4 -0.6510718437803646 1101 5 0.019107503956774963 1101 6 0.4908832721072045 1101 7 0.5853838676006795 1101 8 0.6664004152905534 1101 9 1.359196465665704 1101 10 0.9324519471137194 1101 11 0.88954634692697 1101 12 1.1454135484808816 1101 13 0.9343410207451299 1101 14 0.943853331343341 1101 15 0.8429078128834959 1101 16 1.061681927293506 1101 17 0.9449737210762308 1101 18 0.9787907977429384 1101 19 1.0074497824610944 1101 20 1.293061365762732 1101 21 1.066488975775954 1101 22 1.2137419209618443 1101 23 1.4293083149323778 1101 24 1.3102073601499598 1101 25 1.371073797098532 1102 1 0.49592253854307344 1102 2 0.5264896204154255 1102 3 0.5353707789020135 1102 4 0.530668083516872 1102 5 0.49559380255304575 1102 6 0.5316619281750903 1102 7 0.5033962531297256 1102 8 0.5018097720841439 1102 9 1.2167599557414448 1102 10 0.7219664193945263 1102 11 0.7065437963225563 1102 12 0.8813374345127869 1102 13 0.6134230606356823 1102 14 0.7060252697012643 1102 15 0.7614975759792415 1102 16 0.7500494078284703 1102 17 0.7447395710657361 1102 18 0.8657539243829078 1102 19 0.7286971598795446 1102 20 1.060212210333475 1102 21 1.0689558994536625 1102 22 0.9545345043129316 1102 23 1.2652739828138735 1102 24 1.3616060573052806 1102 25 1.3479775240707284 1103 1 -2.2075869272973776 1103 2 -2.221900185677672 1103 3 -2.066034203787485 1103 4 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-0.425264704237275 2215 2 -0.11661966384674116 2215 3 -0.1316495419972732 2215 4 -0.15748654140546847 2215 5 -0.14358317477516636 2215 6 -0.18701652064640228 2215 7 -0.1654949715785321 2215 8 -0.3211926648487342 2215 9 0.5939323771281195 2215 10 0.24280266946379925 2215 11 0.5367327060174958 2215 12 0.4412803685741409 2215 13 0.4582594536250113 2215 14 0.12665413984526452 2215 15 -0.18907304694805305 2215 16 -0.06595760254636218 2215 17 0.16913795781908902 2215 18 0.16183033119756374 2215 19 0.1357369572830649 2215 20 0.3995613115412908 2215 21 0.4203004418032137 2215 22 0.36929344337939796 2215 23 0.5980123020156256 2215 24 0.7328702654552259 2215 25 0.7324122533538784 2216 1 -0.5465680813329179 2216 2 -0.4823908074602059 2216 3 -0.4404746442727365 2216 4 -0.3090821723046915 2216 5 -1.1043090631679 2216 6 -1.5692349623159672 2216 7 -1.0846662729502263 2216 8 -1.7578691134168771 2216 9 -0.5731098566214463 2216 10 -1.0268465858638862 2216 11 -0.6158024553504582 2216 12 -1.6909136740473194 2216 13 -1.3412027646161135 2216 14 -1.6116252927598858 2216 15 -1.256577191443899 2216 16 -2.297722823541573 2216 17 -2.5848430978241126 2216 18 -2.1490790514062468 2216 19 -3.4532256486689654 2216 20 -2.1641542958278883 2216 21 -2.378701536859321 2216 22 -2.3350439052128067 2216 23 -1.837961921894106 2216 24 -1.8814332587031721 2216 25 -2.137013065504913 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 58244.0 3 4.0 4 709.0 5 8252.0 6 12692.0 7 9795.0 8 7534.0 9 5866.0 10 5131.0 11 4498.0 12 3902.0 13 3491.0 14 3518.0 15 3559.0 16 3531.0 17 3757.0 18 4423.0 19 5943.0 20 8741.0 21 11137.0 22 13263.0 23 15338.0 24 17087.0 25 19103.0 26 21054.0 27 22391.0 28 24590.0 29 28252.0 30 34035.0 31 42016.0 32 51970.0 33 63972.0 34 80413.0 35 101700.0 36 135217.0 37 207824.0 38 446157.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.82162298685038 19.660619381048853 13.554535732901998 20.96322189919877 2 14.970774057678257 23.058005974126445 41.072387931405 20.898832036790303 3 17.375347860189752 31.74103353425953 30.677105158538286 20.20651344701243 4 10.405167359619192 18.72644034172376 38.66681799469322 32.20157430396383 5 10.348760893529748 41.54236191676413 34.330212083328576 13.778665106377547 6 25.390192384540732 40.23392402002761 20.301421476280186 14.074462119151478 7 24.238506142796595 35.48676566696914 23.64682832074056 16.6278998694937 8 26.022572775099817 34.39741894920118 21.52225840601439 18.05774986968461 9 27.025309440236466 15.55158456913491 21.939774616663588 35.48333137396503 10 14.834687924499402 32.586236780475616 32.101593665645964 20.47748162937902 11 30.882416170866854 25.279340868415535 25.06147111462247 18.776771846095137 12 22.632843314701027 30.32295023945746 30.6936039237309 16.35060252211061 13 27.664929791890657 23.919780964398658 28.902411097374003 19.512878146336682 14 21.319324119219353 23.28336758404857 32.45315711004553 22.944151186686547 15 22.5558789594781 29.771554752045304 28.480371680203515 19.19219460827308 16 19.405157273676682 29.923134526332053 29.866719499786765 20.804988700204504 17 18.57375234728226 28.919726304874022 31.547876498701104 20.958644849142622 18 17.930218312924147 25.91545814549538 36.50602603850432 19.648297503076154 19 19.499725293546348 26.313654321219833 36.7922614721848 17.394358913049025 20 20.10014171631215 25.015682824117295 36.751472573527934 18.132702886042622 21 21.114258024348914 24.514401885371914 36.28884230830452 18.082497781974656 22 19.944833496423982 25.163890991418768 36.334671415436546 18.5566040967207 23 19.36930614273386 25.232992723279796 36.5792363305451 18.81846480344125 24 18.862653262835398 25.604169702765635 36.85003697045117 18.6831400639478 25 19.022947707514877 24.9540185377895 37.10051924095631 18.922514513739312 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2995.0 1 2995.0 2 4208.0 3 5421.0 4 5421.0 5 5421.0 6 18397.5 7 31374.0 8 31374.0 9 31374.0 10 26422.0 11 21470.0 12 21470.0 13 21470.0 14 17996.0 15 14522.0 16 14522.0 17 14522.0 18 24180.5 19 33839.0 20 33839.0 21 33839.0 22 49371.0 23 64903.0 24 64903.0 25 64903.0 26 93444.5 27 121986.0 28 121986.0 29 121986.0 30 134577.0 31 147168.0 32 147168.0 33 147168.0 34 151655.0 35 156142.0 36 156142.0 37 156142.0 38 153992.0 39 151842.0 40 151842.0 41 151842.0 42 156684.0 43 161526.0 44 161526.0 45 161526.0 46 164826.5 47 168127.0 48 168127.0 49 168127.0 50 155461.5 51 142796.0 52 142796.0 53 142796.0 54 123136.5 55 103477.0 56 103477.0 57 103477.0 58 87130.0 59 70783.0 60 70783.0 61 70783.0 62 57806.0 63 44829.0 64 44829.0 65 44829.0 66 34042.0 67 23255.0 68 23255.0 69 23255.0 70 17394.5 71 11534.0 72 11534.0 73 11534.0 74 8367.5 75 5201.0 76 5201.0 77 5201.0 78 4193.0 79 3185.0 80 3185.0 81 3185.0 82 2334.0 83 1483.0 84 1483.0 85 1483.0 86 1115.5 87 748.0 88 748.0 89 748.0 90 607.0 91 466.0 92 466.0 93 466.0 94 249.5 95 33.0 96 33.0 97 33.0 98 18.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026660237766342153 2 0.0014102392773128092 3 3.357712565030498E-4 4 0.004700797591042697 5 0.03330850864510254 6 0.011013297213300034 7 0.01960904137977811 8 0.027734705787151915 9 0.035860370194525724 10 0.03968816251866049 11 0.04062832203686903 12 0.007924201653471977 13 0.002417553046821959 14 0.005976728365754287 15 0.005842419863153066 16 0.009670212187287835 17 0.010274600448993323 18 0.01591555755824456 19 0.01658710007125066 20 0.014706781034833583 21 0.011550531223704913 22 0.010878988710698813 23 0.007655584648269535 24 0.005506648606650017 25 0.10429055226984726 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1489109.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.142650340613855 #Duplication Level Percentage of deduplicated Percentage of total 1 76.56496482614486 33.03215505835767 2 11.859757454567198 10.233227379737626 3 4.472381127588352 5.788511255325138 4 2.1415644019910878 3.6957105670802926 5 1.2251284967638671 2.6427645179102695 6 0.7660479830579255 1.982960416632034 7 0.5218180394552354 1.575882925234931 8 0.37470609078245737 1.2932651084100697 9 0.25980653431066164 1.0087868219374414 >10 1.543568261685513 12.522893310757594 >50 0.1589309987291447 4.797959901734113 >100 0.10140260802306085 8.222837098421719 >500 0.005891886284791073 1.8097772270759265 >1k 0.0031009927814689866 2.6727576745535284 >5k 0.0 0.0 >10k+ 9.30297834440696E-4 8.720510736831647 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 32885 2.2083675540205587 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 26803 1.799935397610249 No Hit TATCAACGCAGAGTACTTTTTTTTT 25313 1.69987556317234 No Hit GGTATCAACGCAGAGTACTTTTTTT 21763 1.4614779710551746 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 13325 0.8948303985806279 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 10277 0.6901442406163686 No Hit GTGGTATCAACGCAGAGTACTTTTT 4335 0.2911136793881442 No Hit ATCAACGCAGAGTACTTTTTTTTTT 4181 0.2807719246878503 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 4003 0.2688184679563417 No Hit GTATAACGCAGAGTACTTTTTTTTT 3187 0.21402059889504393 No Hit GTGGTATCAACGCAGAGTACATGGG 2768 0.18588296760008838 No Hit GGTATCAACGCAGAGTACATGGGAT 2272 0.15257445895498584 No Hit ACTTTTTTTTTTTTTTTTTTTTTTT 1928 0.12947339650757603 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 1805 0.121213423597601 No Hit GGTATCAACGCAGAGTACATGGGAG 1672 0.11228190817461985 No Hit CAACGCAGAGTACTTTTTTTTTTTT 1606 0.1078497275887796 No Hit GGTATCAACGCAGAGTACATGGGAA 1534 0.10301462149513568 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 6.715425130060996E-5 0.0 12 0.0 0.0 0.0 6.715425130060996E-5 0.0 13 0.0 0.0 0.0 6.715425130060996E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACTACG 35 0.0021718598 16.283785 16 TATCACG 180 0.0 15.833054 2 ATAACGC 465 0.0 15.730905 3 TATAACG 445 0.0 15.583994 2 TAACGCA 455 0.0 15.033711 4 CGCAGTC 40 0.005289004 14.24592 12 TGGTACG 55 1.9620347E-4 13.816546 17 AGGACCG 70 7.245757E-6 13.57438 5 CGTGCGC 50 0.0015000749 13.299318 10 GTATAAC 575 0.0 12.891059 1 GTATCAC 245 0.0 12.799992 1 GGTATCA 5085 0.0 12.708076 1 GGTACGA 60 4.097097E-4 12.6647415 18 ATAAGGC 125 1.4097168E-9 12.159785 3 AGACGAA 120 1.0017175E-8 11.874391 10 CGAAATC 105 2.7235546E-7 11.760512 13 TTACCGC 65 8.0310495E-4 11.690924 16 GGCGTGC 90 7.464114E-6 11.610517 8 ATCCACG 90 7.464114E-6 11.610517 6 GTATAAA 345 0.0 11.568899 1 >>END_MODULE