FastQCFastQC Report
Thu 26 May 2016
SRR1364279_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364279_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3723127
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT178140.47846877100888585No Hit
TATCAACGCAGAGTACTTTTTTTTT105430.2831759432326644No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN103620.27831443837397973No Hit
GGTATCAACGCAGAGTACTTTTTTT81850.21984208435543562No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA68890.18503263520153893No Hit
GTACTTTTTTTTTTTTTTTTTTTTT52840.14192371090215294No Hit
ACGCAGAGTACTTTTTTTTTTTTTT50130.13464488318555878No Hit
GAGTACTTTTTTTTTTTTTTTTTTT40440.10861837374873326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG1950.013.6320842
CGCATAT2050.013.438918
GGTATCA37600.013.0539381
ATAACGC2000.012.8165923
AGGACCG3200.012.4741965
TAACGCA2000.012.3557284
GTTATCA6650.011.849371
GGACCGA2600.011.6923666
TATCACG1302.6466296E-911.6846432
CGCGCCA2450.011.63255310
GTGGTAT13350.011.164961
GTATCAA89550.011.1105221
CGCAGAT8650.011.0923918
TTGACCG6000.011.09176618
CGAGTGG3000.011.08323910
TGACCGA5500.011.06295719
CGCCAGT2600.010.94874412
AAGACCG1752.0008883E-1110.8618855
TCGCGGT1951.8189894E-1210.72610417
ACGGCCC3900.010.7259615
Produced by FastQC (version 0.11.5)