##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364279_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3723127
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.244510058346115	30.0	25.0	31.0	2.0	34.0
2	25.136157052928894	30.0	24.0	31.0	2.0	34.0
3	24.557438948496788	30.0	20.0	31.0	2.0	34.0
4	28.221246011753024	35.0	26.0	37.0	2.0	37.0
5	28.759020307392145	35.0	28.0	37.0	2.0	37.0
6	28.89123121505122	35.0	28.0	37.0	2.0	37.0
7	28.40896214391827	35.0	27.0	37.0	2.0	37.0
8	27.972897513299976	35.0	26.0	37.0	2.0	37.0
9	28.936706698428498	35.0	26.0	39.0	2.0	39.0
10	28.204115787616164	35.0	23.0	39.0	2.0	39.0
11	28.854531688013864	35.0	27.0	39.0	2.0	39.0
12	28.386879631019838	35.0	25.0	39.0	2.0	39.0
13	28.000362598428687	35.0	22.0	39.0	2.0	39.0
14	28.22883640552686	34.0	17.0	40.0	2.0	41.0
15	28.914895731464437	35.0	25.0	40.0	2.0	41.0
16	29.2487323156046	36.0	26.0	40.0	2.0	41.0
17	29.407655446617856	36.0	27.0	40.0	2.0	41.0
18	29.238042108152637	36.0	26.0	40.0	2.0	41.0
19	29.18809323453108	36.0	25.0	40.0	2.0	41.0
20	29.07204266735999	36.0	25.0	40.0	2.0	41.0
21	28.960549559550344	36.0	24.0	40.0	2.0	41.0
22	29.02945695916363	36.0	25.0	40.0	2.0	41.0
23	29.01772381119419	36.0	25.0	40.0	2.0	41.0
24	28.85564070202279	36.0	25.0	40.0	2.0	41.0
25	28.653439165518662	36.0	24.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.25466380063673455
1101	2	0.29376494917048035
1101	3	1.0117419885937622
1101	4	0.4337950001319193
1101	5	-0.03179492037215326
1101	6	-0.19715966236027782
1101	7	0.08538280044400892
1101	8	0.37461497746252803
1101	9	0.19636318376998219
1101	10	0.6627967921484306
1101	11	-0.02123889690311742
1101	12	0.3370364934219161
1101	13	0.2268778817519994
1101	14	0.725816745575699
1101	15	-0.05178120496415772
1101	16	-0.5810939887286288
1101	17	-1.2506146099185713
1101	18	-6.929402603530772
1101	19	-8.322817538394517
1101	20	-9.629967903885426
1101	21	-10.99224281280286
1101	22	-11.399180352721118
1101	23	-12.589766423978979
1101	24	-12.214764050280671
1101	25	-12.785722120462061
1102	1	1.4715428397783015
1102	2	1.6745847969242362
1102	3	2.4926850791576314
1102	4	1.7983994286610425
1102	5	1.1882529707511296
1102	6	1.3519114439507973
1102	7	1.6680196242260763
1102	8	1.9063391449980962
1102	9	2.0076521878486275
1102	10	-1.6786290917034634
1102	11	0.5382952191454464
1102	12	1.584144113448346
1102	13	2.123450163877873
1102	14	2.554684554130656
1102	15	2.1863187405377573
1102	16	1.7611166426279254
1102	17	1.5938654062068913
1102	18	1.7603317867501538
1102	19	1.428854171625911
1102	20	1.8670888885913506
1102	21	1.8679501328060617
1102	22	1.833617601236714
1102	23	1.8422013051929227
1102	24	2.0031112586040116
1102	25	2.1107330778835838
1103	1	-0.6121982530792387
1103	2	-0.17963707564216236
1103	3	-2.8296332098205
1103	4	-0.588202922934677
1103	5	-3.2070468373895835
1103	6	-7.575764286893364
1103	7	-8.812341851886814
1103	8	-3.0820906349959145
1103	9	-7.622085394359832
1103	10	-5.289059812578763
1103	11	-8.516743678588199
1103	12	-2.654324490239013
1103	13	-0.5732099733206901
1103	14	-0.16340531256264157
1103	15	-0.47650441212839567
1103	16	-0.611181883286747
1103	17	-0.8367998439486897
1103	18	-0.5841414469236774
1103	19	-6.145618595024516
1103	20	-2.34375167379595
1103	21	-6.807726035445384
1103	22	-3.1642963609024974
1103	23	-2.3038184973586944
1103	24	-6.866459357042942
1103	25	-4.2069753483956305
1104	1	1.5021918711712843
1104	2	1.44349023486836
1104	3	2.326349150886138
1104	4	1.914185191795383
1104	5	1.4643497183928922
1104	6	1.3522748081063725
1104	7	1.5153413873565746
1104	8	1.6704755409201582
1104	9	1.784593404589856
1104	10	2.1019145610838734
1104	11	1.6786583816654215
1104	12	1.8924334250091945
1104	13	2.076725734212573
1104	14	2.6137814882853476
1104	15	1.6178438306076615
1104	16	0.8136745822577467
1104	17	0.5860680495055917
1104	18	0.6362870654141553
1104	19	1.2484572807508592
1104	20	-9.011753259778363
1104	21	-3.5190231511969614
1104	22	-1.248636041466444
1104	23	-0.15209798829033616
1104	24	0.4034753068803738
1104	25	0.923330970167445
1105	1	1.1394167743458645
1105	2	1.2254910837321518
1105	3	1.9677440458886721
1105	4	1.1795074076573933
1105	5	1.1890659682635558
1105	6	1.1677629878782092
1105	7	1.4468823056117692
1105	8	1.6016858535356597
1105	9	1.4780986868200863
1105	10	2.03825481063139
1105	11	1.7016820531747854
1105	12	1.8736307523619082
1105	13	1.8132043213868698
1105	14	2.5327645171958437
1105	15	1.9591629186043171
1105	16	1.4527457459859612
1105	17	0.8027781661594666
1105	18	1.4228070707853604
1105	19	1.2857918824065422
1105	20	1.1296360857073786
1105	21	1.036225351312936
1105	22	1.0960648250223741
1105	23	1.1338207891032006
1105	24	1.0410475248954754
1105	25	1.0913373750982807
1106	1	0.845412229685838
1106	2	1.0354893357859893
1106	3	1.6905197843959279
1106	4	1.3445135254689653
1106	5	0.6000081112455504
1106	6	0.3490250050080803
1106	7	0.9027466649895004
1106	8	0.8196547400939558
1106	9	1.1054365648134414
1106	10	-2.4191827002932733
1106	11	-0.2645825858743329
1106	12	0.3850797997312476
1106	13	1.4157213638619623
1106	14	0.3037835698090241
1106	15	1.0902588808486797
1106	16	0.6805031310585008
1106	17	-5.8196654625767685
1106	18	-1.87679090159709
1106	19	-0.18545440320786
1106	20	0.4341283073469526
1106	21	0.7818991845588741
1106	22	0.8702301807294184
1106	23	0.2927090953433691
1106	24	0.6755519821581935
1106	25	0.701020996842729
1107	1	0.6724101769501054
1107	2	1.0254894739815583
1107	3	1.6029126315754674
1107	4	0.970003895743492
1107	5	0.5192056171648005
1107	6	0.8126658568941707
1107	7	0.6954642747953699
1107	8	0.9566820258221043
1107	9	1.0650690010449182
1107	10	-2.0520295396618806
1107	11	-0.019640505763728555
1107	12	0.33618049707216713
1107	13	1.2025154255831403
1107	14	1.6253973417416852
1107	15	1.4512997881041159
1107	16	-5.372868423221
1107	17	-1.8599861917148175
1107	18	-0.08050064777131283
1107	19	0.7988434820506356
1107	20	1.1173114403194901
1107	21	1.316295742972848
1107	22	0.9798096090887576
1107	23	0.6326944952092148
1107	24	0.9435191754411001
1107	25	1.118851475222641
1108	1	-0.5929678313606459
1108	2	-3.000742037184395
1108	3	-0.24550076361427742
1108	4	-1.0083332237338212
1108	5	-1.3177563788761049
1108	6	-0.821052177070051
1108	7	-0.5025844789723877
1108	8	-0.3057573971421661
1108	9	-0.7819876207002778
1108	10	-3.884020605072145
1108	11	-2.057989706413128
1108	12	-6.343891198750089
1108	13	-2.396563575240023
1108	14	-6.41860110018272
1108	15	-3.3043568327281463
1108	16	-7.885857813395688
1108	17	-3.923608474021684
1108	18	-2.4070894208019986
1108	19	-1.1471932392348982
1108	20	-1.1841518421988724
1108	21	-0.5632330608846061
1108	22	-0.7125189987919995
1108	23	-0.6750604304550585
1108	24	-0.45699495907377496
1108	25	-0.7627770789471136
1109	1	-8.006931736492884
1109	2	-5.8601851788626895
1109	3	-5.094191434159558
1109	4	-4.890846507001356
1109	5	-2.2589763616598013
1109	6	-1.3676285076230528
1109	7	-0.975498189679147
1109	8	-0.5228030203426179
1109	9	-5.311275518827262
1109	10	-1.3931707727826748
1109	11	-1.0962084128158125
1109	12	-5.720899124185692
1109	13	-7.585968722320672
1109	14	-7.708864390871074
1109	15	-3.7934425877223603
1109	16	-2.1123804172129574
1109	17	-1.7150104153081571
1109	18	-1.0378107244916315
1109	19	-0.7123011379555493
1109	20	-0.4494624777178835
1109	21	-0.3382207423236494
1109	22	-0.5544522707798905
1109	23	-0.8321051631571308
1109	24	-0.5906590630839865
1109	25	-0.3844277661150315
1110	1	-2.2157125369486614
1110	2	-2.098983070900861
1110	3	-1.4196237087643162
1110	4	-2.284227791718635
1110	5	-5.208598922548564
1110	6	-3.9223543301554535
1110	7	-8.693098246651086
1110	8	-9.477386413021723
1110	9	-10.929552117602366
1110	10	-8.918825237958576
1110	11	-11.502684698245236
1110	12	-6.596888299516841
1110	13	-4.683275373794821
1110	14	-4.258860117975477
1110	15	-9.762291754507718
1110	16	-11.33963579393992
1110	17	-11.957482997561396
1110	18	-8.925560332809535
1110	19	-12.044612774474562
1110	20	-13.16472668509575
1110	21	-14.009170749445143
1110	22	-14.661503972816638
1110	23	-15.347493284507363
1110	24	-15.583362381699567
1110	25	-16.32595034124678
1111	1	-0.5358376010588124
1111	2	0.04792938422203363
1111	3	-2.5045355326487986
1111	4	-3.0978002066000805
1111	5	-1.0300637901857606
1111	6	-0.46738569481399495
1111	7	-0.0797372966372798
1111	8	0.2785002796835272
1111	9	-4.2675791030444294
1111	10	-4.067199010002604
1111	11	-1.349696573417841
1111	12	-5.5715519678010565
1111	13	-1.1979954590120059
1111	14	0.26344153508011203
1111	15	0.3096836271942607
1111	16	0.20805939681243757
1111	17	0.2834082321699327
1111	18	0.5306096281325878
1111	19	0.5282747467311033
1111	20	0.5374033500168878
1111	21	0.7845567133709679
1111	22	0.2952952539163114
1111	23	0.22512556584968024
1111	24	0.4011000793411874
1111	25	0.6109920977006311
1112	1	-0.20400828649531633
1112	2	0.373825682092491
1112	3	-2.2538867097298443
1112	4	-0.343853773375006
1112	5	-0.4908908695281333
1112	6	-0.1797111915772618
1112	7	0.2784972577561753
1112	8	-3.431380115108599
1112	9	-0.7961452188212554
1112	10	-2.9740359316505973
1112	11	-0.7261980036855178
1112	12	0.3820647326830482
1112	13	0.9291542283818011
1112	14	1.5619579018256609
1112	15	1.0421382503270173
1112	16	0.7487075656748878
1112	17	0.5721931603996779
1112	18	0.6170805372722583
1112	19	0.6890018335079944
1112	20	0.9162395809449997
1112	21	0.7988363906472706
1112	22	0.7350943601220727
1112	23	0.47309442619696185
1112	24	0.7671921664230403
1112	25	0.724168145155712
1113	1	-2.3415952153912443
1113	2	-1.7678509227543167
1113	3	-4.0764745911443825
1113	4	-2.8261890462010584
1113	5	-2.9467854457052773
1113	6	-2.8806857014386367
1113	7	-2.686909488384707
1113	8	-9.45923385051189
1113	9	-10.64545481438153
1113	10	-5.245484020257809
1113	11	-10.175588767555336
1113	12	-8.745186198405253
1113	13	-4.406996722889655
1113	14	-2.9891499769791174
1113	15	-7.726390142733248
1113	16	-4.360366761601355
1113	17	-3.7894930230047557
1113	18	-3.190304624328597
1113	19	-2.9327377236149665
1113	20	-7.860015486219815
1113	21	-4.3693284006269835
1113	22	-3.7069682734497356
1113	23	-3.645008840762145
1113	24	-8.878596715588063
1113	25	-6.413270759407588
1114	1	-4.049772769391538
1114	2	-3.8383801895892873
1114	3	-0.7114869829010075
1114	4	-0.5244122312996353
1114	5	-0.6326098151759929
1114	6	-0.734017293551549
1114	7	-0.5220852211625626
1114	8	-0.11293847877844243
1114	9	-0.4286692356225359
1114	10	-3.291393414334493
1114	11	-1.26657491437145
1114	12	-4.565042400123286
1114	13	-1.1990851649080874
1114	14	-5.575287659170879
1114	15	-2.015883759898344
1114	16	-1.0797066294311755
1114	17	-0.9731973389639634
1114	18	-0.5430293562041797
1114	19	-0.287153942035026
1114	20	-0.044219921754230285
1114	21	0.10000740292912624
1114	22	-0.03336624456430215
1114	23	-0.4335898145706807
1114	24	-0.30175755686704875
1114	25	-0.15031930951002792
1115	1	1.2863722741620975
1115	2	1.3422319550334691
1115	3	2.04669995378055
1115	4	1.0405638816046867
1115	5	0.8923837594976369
1115	6	0.24685539175612448
1115	7	-1.5781622570461202
1115	8	0.5502840239743669
1115	9	0.6521805830824725
1115	10	1.6435929916906993
1115	11	0.5875115931990962
1115	12	1.3291520025355403
1115	13	1.5602495114444466
1115	14	2.2299203828179976
1115	15	1.4351161972661473
1115	16	1.0615998409984648
1115	17	0.6877104714611946
1115	18	1.1169250491759577
1115	19	1.255041239222173
1115	20	1.2949903804376888
1115	21	1.3162886130070603
1115	22	1.0208121703706752
1115	23	0.8014042136553989
1115	24	0.7702379101281913
1115	25	1.0861982139928088
1116	1	0.9717068725095537
1116	2	1.165251833913043
1116	3	1.9578701883661687
1116	4	1.3458746938257988
1116	5	0.5640556623696469
1116	6	-0.42241293903451194
1116	7	-3.849974777368086
1116	8	-0.3121566238190354
1116	9	-0.4281073207402599
1116	10	0.45557566757260304
1116	11	-1.1841099232614702
1116	12	0.3870125240205695
1116	13	-5.709261612174039
1116	14	-0.8282432715224743
1116	15	-0.42925085228295146
1116	16	-0.02571154551613475
1116	17	0.4294005592882826
1116	18	0.5872110165679132
1116	19	0.9116383904369059
1116	20	0.9095434570401153
1116	21	1.0087807662396635
1116	22	0.9116783853241444
1116	23	0.8018900556921125
1116	24	0.8516679159978082
1116	25	0.8467386410572324
1201	1	1.9975710362393109
1201	2	2.1691609134494456
1201	3	2.827309690571287
1201	4	2.422273869272235
1201	5	1.972354790377345
1201	6	1.8565422589983065
1201	7	2.0707337815469913
1201	8	2.247976231681296
1201	9	2.5869462035145823
1201	10	2.902336799603077
1201	11	2.365455707668726
1201	12	2.6523861725146958
1201	13	2.765526267514648
1201	14	3.4456895059905044
1201	15	2.8569416251464865
1201	16	2.3611069701635934
1201	17	2.2209865052148743
1201	18	2.2007239978141264
1201	19	2.262543143960947
1201	20	2.441882507907213
1201	21	2.2170250745196434
1201	22	1.4112922769820777
1201	23	0.927815564369304
1201	24	1.3725218201003848
1201	25	1.514889696684648
1202	1	1.9126186250675055
1202	2	2.114128443581947
1202	3	2.8131631416418763
1202	4	2.2886461927963566
1202	5	1.8693911704226274
1202	6	1.7505858273730013
1202	7	1.8980527649401076
1202	8	2.205012467888686
1202	9	2.329347075541026
1202	10	-1.536237474289102
1202	11	0.8539069694282411
1202	12	1.973541271930653
1202	13	2.53889104274581
1202	14	3.0849664464282185
1202	15	2.623639944345392
1202	16	2.3351634089562054
1202	17	1.8732743821270113
1202	18	2.1459204084563126
1202	19	2.243871694663863
1202	20	2.2593765948789795
1202	21	2.4663812770124167
1202	22	1.9965216375660937
1202	23	1.7075104055187929
1202	24	1.979267362385837
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2211	17	-0.0224445180057522
2211	18	0.35693046555759267
2211	19	0.6753883727420025
2211	20	0.951525740789279
2211	21	1.0065879909110684
2211	22	0.8773524434700128
2211	23	0.6902010013981261
2211	24	0.9072673445936807
2211	25	0.9335016716402862
2212	1	0.9064764585405456
2212	2	1.0433785713037054
2212	3	1.630994751153711
2212	4	1.177966699156734
2212	5	0.9068092243614245
2212	6	0.715340813297022
2212	7	1.0470785216039005
2212	8	1.3914029992009311
2212	9	1.1469715926991952
2212	10	1.931008297635497
2212	11	1.0496098544532266
2212	12	1.315398468051221
2212	13	1.4512187072163307
2212	14	-4.226940625382799
2212	15	-0.9126194169078268
2212	16	-0.266864974440459
2212	17	0.1310632438234407
2212	18	0.5118692003116152
2212	19	0.9935645891139266
2212	20	1.340431192769291
2212	21	1.207204127481102
2212	22	1.013127034899803
2212	23	1.0436357883751946
2212	24	1.1248619216457492
2212	25	1.0483912427982816
2213	1	0.9175131259067939
2213	2	1.0001635788492713
2213	3	1.6496254611774326
2213	4	1.11081592672452
2213	5	0.5876187889001798
2213	6	0.3714776325664566
2213	7	0.6020567996538446
2213	8	-5.078690057110169
2213	9	-1.4229962161230318
2213	10	0.3960602963620694
2213	11	0.380017881571721
2213	12	0.9244790810657193
2213	13	-7.421377953884043
2213	14	-1.9299904537968295
2213	15	-0.5443583306927025
2213	16	0.07995954330399968
2213	17	0.2886301517320149
2213	18	0.5769306451141141
2213	19	0.8899914044722479
2213	20	1.046981989302246
2213	21	1.061057909651737
2213	22	0.741370077500072
2213	23	0.656267767621248
2213	24	0.7476703210299931
2213	25	0.8486835414655474
2214	1	-0.7657318141472906
2214	2	-0.4013277771575261
2214	3	-6.663489254982803
2214	4	-2.4904799674218765
2214	5	-1.5632275089497192
2214	6	-0.9946752674390531
2214	7	-0.479761794029951
2214	8	-5.932270656251308
2214	9	-1.7567602926318422
2214	10	-0.017238892952867957
2214	11	-0.42901173685578087
2214	12	-0.0980748765975612
2214	13	-6.091320890442461
2214	14	-1.398349552947682
2214	15	-0.7493633047313715
2214	16	-0.6878860606390269
2214	17	-0.5516275952668472
2214	18	-0.5053656892505529
2214	19	-0.04257201162153734
2214	20	0.16172649751359813
2214	21	0.20177009014236091
2214	22	0.022462638326707918
2214	23	-0.07317388191336605
2214	24	0.17849234682881487
2214	25	0.2262597989340165
2215	1	0.38530436419381786
2215	2	0.6229383718335377
2215	3	-2.5036129449082125
2215	4	-0.5085795998576188
2215	5	-0.046433876754967685
2215	6	-0.5098559825017865
2215	7	0.28542880461886
2215	8	0.5294004815113702
2215	9	0.831621520392158
2215	10	1.6118857430630449
2215	11	0.7276873157288897
2215	12	0.5357809479574307
2215	13	1.1070579280652488
2215	14	1.1127610698254884
2215	15	0.7879943374571603
2215	16	0.599986526898526
2215	17	0.49475097467918516
2215	18	0.5154386285727703
2215	19	0.5756015584496197
2215	20	0.8177981000992283
2215	21	0.9782789720164757
2215	22	0.7473072820223763
2215	23	0.7202774642415726
2215	24	0.5882937414238398
2215	25	0.6986025810457335
2216	1	0.5254809944595848
2216	2	0.22592645230367125
2216	3	1.401614108176716
2216	4	-2.943652162841204E-4
2216	5	-0.14661587446972035
2216	6	-0.4372491520150845
2216	7	-1.0043881535425712
2216	8	-6.088137270600175
2216	9	-1.8985017881833421
2216	10	-0.5899521595695525
2216	11	-1.0878428738264851
2216	12	0.1556608410897269
2216	13	0.7841664106943504
2216	14	1.4975836085432093
2216	15	1.0352899650051413
2216	16	0.39999032000457646
2216	17	0.2493453233998153
2216	18	0.4195045527559209
2216	19	0.5549930116843953
2216	20	0.6621992818352993
2216	21	0.46769477862353526
2216	22	0.285938537664876
2216	23	0.01022891452343444
2216	24	0.14784657704591808
2216	25	0.1091219857401704
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	434938.0
3	2.0
4	2854.0
5	19155.0
6	27899.0
7	24913.0
8	21652.0
9	18230.0
10	16148.0
11	14882.0
12	13861.0
13	13284.0
14	13565.0
15	13807.0
16	14096.0
17	14961.0
18	17311.0
19	22522.0
20	30837.0
21	38587.0
22	47112.0
23	54617.0
24	62350.0
25	68919.0
26	75956.0
27	82521.0
28	89711.0
29	103210.0
30	116577.0
31	130066.0
32	159240.0
33	195813.0
34	239746.0
35	292431.0
36	354460.0
37	401374.0
38	475520.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.12786827102896	17.06680048629997	12.335131938917662	23.470199303753407
2	15.04094446085481	20.139570158624746	39.74970838660713	25.06977699391331
3	16.892257233624246	25.91228598860475	29.510998213999805	27.6844585637712
4	10.98498907921752	15.674658528439721	35.602478665861256	37.7378737264815
5	12.969438023546537	38.97594802779219	32.8585657773255	15.196048171335763
6	33.20177365790946	35.634208784396684	16.698721428538715	14.465296129155142
7	29.802473074185222	32.48206786808762	19.58472787508211	18.13073118264505
8	27.096140608013535	34.310478379241275	19.5630719407694	19.030309071975797
9	27.22608248172626	15.979049522983289	18.697223037980823	38.097644957309626
10	13.867257778163797	30.676928040015472	31.884227238422103	23.57158694339863
11	35.07610950025676	24.115279933915758	21.080922485185738	19.727688080641745
12	23.12859665056675	27.208116794504168	28.550875320244863	21.11241123468422
13	28.097791796407538	22.0760551374692	25.966287757759805	23.859865308363457
14	21.179367017910273	21.548176844433055	27.643494072343195	29.628962065313473
15	23.718647602868245	29.383718615274905	23.100402294530888	23.797231487325956
16	23.21065491025761	29.600373512409504	23.94332008456295	23.245651492769934
17	22.673768613110763	28.90094756675181	25.417116330292234	23.008167489845192
18	21.84858306422783	27.64377803921294	27.893159166150543	22.614479730408686
19	23.28001390121784	26.647799445298304	27.671855826051583	22.400330827432274
20	23.43342954525762	26.55330341253159	27.625814883866816	22.387452158343972
21	24.013294635888673	25.949379194816203	27.71387921946683	22.323446949828295
22	23.70266596799114	26.241040361982172	27.154778630766597	22.901515039260087
23	23.049610234548933	26.402649155059194	27.377413000515087	23.170327609876786
24	23.287518814736906	26.273441471653904	27.293741047256475	23.14529866635272
25	22.70229308690412	26.24658078927533	27.94887225984978	23.102253863970773
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	18925.0
1	18925.0
2	15712.0
3	12499.0
4	12499.0
5	12499.0
6	17052.5
7	21606.0
8	21606.0
9	21606.0
10	22869.5
11	24133.0
12	24133.0
13	24133.0
14	27059.0
15	29985.0
16	29985.0
17	29985.0
18	39871.5
19	49758.0
20	49758.0
21	49758.0
22	66816.0
23	83874.0
24	83874.0
25	83874.0
26	113903.5
27	143933.0
28	143933.0
29	143933.0
30	174951.5
31	205970.0
32	205970.0
33	205970.0
34	234995.5
35	264021.0
36	264021.0
37	264021.0
38	298264.0
39	332507.0
40	332507.0
41	332507.0
42	366086.5
43	399666.0
44	399666.0
45	399666.0
46	428393.0
47	457120.0
48	457120.0
49	457120.0
50	460151.0
51	463182.0
52	463182.0
53	463182.0
54	443466.0
55	423750.0
56	423750.0
57	423750.0
58	375497.5
59	327245.0
60	327245.0
61	327245.0
62	274163.0
63	221081.0
64	221081.0
65	221081.0
66	173449.0
67	125817.0
68	125817.0
69	125817.0
70	95057.5
71	64298.0
72	64298.0
73	64298.0
74	46666.5
75	29035.0
76	29035.0
77	29035.0
78	22264.5
79	15494.0
80	15494.0
81	15494.0
82	10681.0
83	5868.0
84	5868.0
85	5868.0
86	4151.5
87	2435.0
88	2435.0
89	2435.0
90	1596.0
91	757.0
92	757.0
93	757.0
94	440.5
95	124.0
96	124.0
97	124.0
98	84.0
99	44.0
100	44.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3400367486792688
2	0.3420511843941934
3	0.353493179254965
4	0.3552927418269643
5	0.36130918982887233
6	0.3021653572386867
7	0.3124524089562349
8	0.3097396355268031
9	0.30933674838381825
10	0.4233269507056837
11	0.3089070020979677
12	0.30925617095522123
13	0.30192362495289576
14	0.3077520589547442
15	0.30001662580943383
16	0.30447524352513355
17	0.2979484718087779
18	0.2991571332377327
19	0.30141330123844823
20	0.49576605901437154
21	0.3022727938101494
22	0.30463639838232753
23	0.2995063020949863
24	0.30286369495319393
25	0.29579974037952506
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3723127.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.266234631504645
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.76913502929285	50.557107803894866
2	6.267398329626253	7.30355403206207
3	2.40038853737101	4.195848051757006
4	1.2829757411774745	2.9901666224790127
5	0.756583254542739	2.2041628713727315
6	0.49404268843665544	1.7271604321457708
7	0.358954127426045	1.4640433787387068
8	0.24796553602843538	1.1558414482207708
9	0.1945737020937625	1.0203369281384087
>10	1.0668535270867106	11.398270977012684
>50	0.09249815696043566	3.753735063893731
>100	0.062017671696931766	7.031814548947864
>500	0.004578714193771126	1.8275542230041495
>1k	0.001711236601768298	1.6433112635983909
>5k	1.849985515425187E-4	0.6836957424239479
>10k+	1.3874891365688903E-4	1.0433966123098355
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	17814	0.47846877100888585	No Hit
TATCAACGCAGAGTACTTTTTTTTT	10543	0.2831759432326644	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	10362	0.27831443837397973	No Hit
GGTATCAACGCAGAGTACTTTTTTT	8185	0.21984208435543562	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	6889	0.18503263520153893	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	5284	0.14192371090215294	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	5013	0.13464488318555878	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	4044	0.10861837374873326	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.685914286566104E-5	0.0
2	0.0	0.0	0.0	2.685914286566104E-5	0.0
3	0.0	0.0	0.0	5.371828573132208E-5	0.0
4	0.0	0.0	0.0	5.371828573132208E-5	0.0
5	0.0	0.0	0.0	5.371828573132208E-5	0.0
6	5.371828573132208E-5	0.0	0.0	5.371828573132208E-5	0.0
7	5.371828573132208E-5	0.0	0.0	5.371828573132208E-5	0.0
8	8.057742859698312E-5	0.0	0.0	5.371828573132208E-5	0.0
9	8.057742859698312E-5	0.0	0.0	5.371828573132208E-5	0.0
10	8.057742859698312E-5	0.0	0.0	5.371828573132208E-5	0.0
11	8.057742859698312E-5	0.0	0.0	5.371828573132208E-5	0.0
12	1.342957143283052E-4	0.0	0.0	5.371828573132208E-5	0.0
13	1.6115485719396625E-4	0.0	0.0	8.057742859698312E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAACG	195	0.0	13.632084	2
CGCATAT	205	0.0	13.43891	8
GGTATCA	3760	0.0	13.053938	1
ATAACGC	200	0.0	12.816592	3
AGGACCG	320	0.0	12.474196	5
TAACGCA	200	0.0	12.355728	4
GTTATCA	665	0.0	11.84937	1
GGACCGA	260	0.0	11.692366	6
TATCACG	130	2.6466296E-9	11.684643	2
CGCGCCA	245	0.0	11.632553	10
GTGGTAT	1335	0.0	11.16496	1
GTATCAA	8955	0.0	11.110522	1
CGCAGAT	865	0.0	11.092391	8
TTGACCG	600	0.0	11.091766	18
CGAGTGG	300	0.0	11.083239	10
TGACCGA	550	0.0	11.062957	19
CGCCAGT	260	0.0	10.948744	12
AAGACCG	175	2.0008883E-11	10.861885	5
TCGCGGT	195	1.8189894E-12	10.726104	17
ACGGCCC	390	0.0	10.72596	15
>>END_MODULE