FastQCFastQC Report
Thu 26 May 2016
SRR1364279_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364279_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3723127
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT402181.0802210077711558No Hit
TATCAACGCAGAGTACTTTTTTTTT299600.8046999202552049No Hit
GGTATCAACGCAGAGTACTTTTTTT282810.75960341938376No Hit
ACGCAGAGTACTTTTTTTTTTTTTT165190.44368618099785473No Hit
GTACTTTTTTTTTTTTTTTTTTTTT119980.3222559961022012No Hit
GAGTACTTTTTTTTTTTTTTTTTTT108300.2908845172351091No Hit
GTGGTATCAACGCAGAGTACATGGG49680.13343622175660408No Hit
GTGGTATCAACGCAGAGTACTTTTT46200.12408924003935401No Hit
ATCAACGCAGAGTACTTTTTTTTTT45590.12245083232454868No Hit
TGCATGCACTGCCTCAGTGACCAGT41760.11216378060700052No Hit
GCAGAGTACTTTTTTTTTTTTTTTT41600.11173403432114995No Hit
GTCCCAGGGTGTGCTTGTCAAAGAG38240.10270936231828784No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC402.75887E-416.62783
TATCACG1150.016.52232
CGTTGCG350.002176910416.27904712
TATAACG2750.015.5459822
TAACGCA2800.014.9316864
ATAACGC3000.014.8858393
AGGACCG3550.014.1889185
GGTATCA68450.013.4783481
TCGACCG500.001503954913.29562718
GTGGTAT20450.012.9164261
GGACCGA2750.012.7843146
TTGACCG5300.012.18467118
TGGTATC20100.012.1471792
TGACCGA5500.011.57965619
ACCGAGT3450.011.2917568
GTATAAC4800.011.2829981
TACAACG1354.7348294E-911.2596422
CGCGAAT1104.995891E-711.22358218
CCGTAAG855.3188538E-511.1782021
CGACCGT600.005852136811.0899719
Produced by FastQC (version 0.11.5)