##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364279_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3723127
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.676919159620393	31.0	30.0	34.0	26.0	34.0
2	29.70720821503	31.0	30.0	34.0	26.0	34.0
3	29.730578086645984	31.0	30.0	34.0	26.0	34.0
4	33.10367548568716	35.0	33.0	37.0	27.0	37.0
5	32.65452642362187	35.0	33.0	37.0	26.0	37.0
6	32.507249416955155	35.0	33.0	37.0	25.0	37.0
7	32.22811282021806	35.0	32.0	37.0	22.0	37.0
8	32.11845499764043	35.0	32.0	37.0	20.0	37.0
9	32.85969562682122	37.0	32.0	39.0	17.0	39.0
10	33.006813358770735	37.0	32.0	39.0	17.0	39.0
11	32.93079231516948	37.0	32.0	39.0	17.0	39.0
12	32.876892730223815	37.0	32.0	39.0	17.0	39.0
13	32.914520777830035	37.0	32.0	39.0	17.0	39.0
14	33.84530906412808	38.0	33.0	41.0	17.0	41.0
15	33.88335960605158	38.0	33.0	41.0	17.0	41.0
16	33.848528938174816	38.0	33.0	41.0	17.0	41.0
17	33.83088140694637	38.0	33.0	41.0	17.0	41.0
18	33.78680501632096	38.0	33.0	41.0	17.0	41.0
19	33.69646348351802	39.0	32.0	41.0	16.0	41.0
20	33.60296304692265	39.0	32.0	41.0	11.0	41.0
21	33.55885254518581	38.0	32.0	41.0	10.0	41.0
22	33.487593896206064	38.0	33.0	41.0	10.0	41.0
23	33.22721760498634	38.0	32.0	41.0	2.0	41.0
24	33.135862676723086	38.0	32.0	41.0	2.0	41.0
25	32.95790688848379	38.0	32.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.288201085304415
1101	2	0.4769951470809346
1101	3	-1.5634237260188613
1101	4	-0.4429637564125102
1101	5	0.3943172043178649
1101	6	0.7499860116725756
1101	7	0.9075164856897544
1101	8	0.974422654767185
1101	9	1.7214719890688812
1101	10	1.429313809858769
1101	11	1.2981139647655482
1101	12	1.4157447155618428
1101	13	1.2400530436870625
1101	14	1.5230223803470935
1101	15	1.3728431790375382
1101	16	1.3829293382669832
1101	17	1.3084129691835003
1101	18	1.2703527344320094
1101	19	1.2673017560904754
1101	20	1.406444910137445
1101	21	1.3114369525464369
1101	22	1.423201158805135
1101	23	1.7103238785994606
1101	24	1.7039440457698163
1101	25	1.73676026811534
1102	1	0.6556670364452657
1102	2	0.6361676223141686
1102	3	0.8348342017102119
1102	4	0.7702992478502821
1102	5	0.9417941795000857
1102	6	1.0752955184773754
1102	7	1.1494337854715155
1102	8	1.1532513317080237
1102	9	1.7956817046585698
1102	10	1.39344225113895
1102	11	1.4325008862211206
1102	12	1.5589973427924662
1102	13	1.4277856870431478
1102	14	1.3932399869605234
1102	15	1.4318813998617799
1102	16	1.3988363082980158
1102	17	1.3717595869429076
1102	18	1.520251825881779
1102	19	1.2943514687935078
1102	20	1.574412666682818
1102	21	1.7105410936541716
1102	22	1.7296201121931034
1102	23	2.0262570400606066
1102	24	1.891359654617915
1102	25	1.8083056744744468
1103	1	-2.3966671123438346
1103	2	-2.3186535589816586
1103	3	-2.320078599704985
1103	4	-2.277582801023147
1103	5	-4.96516146960359
1103	6	-9.210131339013284
1103	7	-10.03350630529809
1103	8	-10.762488913672904
1103	9	-11.455927342837708
1103	10	-9.402189637218914
1103	11	-11.959127662349882
1103	12	-11.19538365945494
1103	13	-12.093155186655117
1103	14	-12.0791771553043
1103	15	-12.094561996712095
1103	16	-6.583826302226111
1103	17	-4.606683296284917
1103	18	-3.798950505742045
1103	19	-3.363518198264373
1103	20	-7.875708664478161
1103	21	-4.858681266818792
1103	22	-10.385163038969466
1103	23	-12.050067303515224
1103	24	-12.985169230020091
1103	25	-13.4254146681157
1104	1	0.30576769821810856
1104	2	0.5239801544948506
1104	3	0.5833246351811461
1104	4	0.6655004645771072
1104	5	0.63879084123068
1104	6	0.7766376674471971
1104	7	0.5706025470280593
1104	8	0.7947593763683933
1104	9	1.1190268944852875
1104	10	0.8397180055861568
1104	11	0.8546993695853402
1104	12	0.8075885340531954
1104	13	0.6304115826712788
1104	14	0.790565278714034
1104	15	0.743438888451962
1104	16	0.40277816427210666
1104	17	0.46501023127667196
1104	18	0.6365500194908407
1104	19	0.9303250362865825
1104	20	1.074057704010606
1104	21	0.9414381008297568
1104	22	0.7245651196836249
1104	23	1.179923951490487
1104	24	1.366939242950032
1104	25	1.5076962188689649
1105	1	0.23206192741206166
1105	2	0.3126464582291817
1105	3	-0.11193860053770521
1105	4	0.1661442691754118
1105	5	0.400657376018998
1105	6	0.49741194088201723
1105	7	0.564858645522456
1105	8	0.2890647533588968
1105	9	0.44048411025814005
1105	10	0.15147154250851713
1105	11	0.7597229680715571
1105	12	0.8772561671242443
1105	13	0.7516711325405723
1105	14	0.5841172799910908
1105	15	0.387054924481248
1105	16	0.6404410425899414
1105	17	0.6181257967190561
1105	18	0.6752452231678134
1105	19	0.740736621203105
1105	20	0.9551949085655522
1105	21	0.9430209564497645
1105	22	0.7683433774895505
1105	23	1.2553632639763137
1105	24	1.1010227435876487
1105	25	1.1830800240567854
1106	1	-0.09707633512945435
1106	2	0.056222756079208125
1106	3	-0.12364983983153266
1106	4	-1.3640078021407938
1106	5	-0.3593496157656588
1106	6	-0.07154453525851778
1106	7	0.2140458505565448
1106	8	0.34132834364030984
1106	9	0.8332476131422553
1106	10	0.2762748985651484
1106	11	0.2820092816530959
1106	12	0.4189446831179495
1106	13	0.22833605126107415
1106	14	-0.2557708514544643
1106	15	0.26714581768173673
1106	16	0.11430924744923487
1106	17	-0.01900057978854619
1106	18	0.08863449077836805
1106	19	-1.3424656161679849
1106	20	-0.11542211643007505
1106	21	-0.4766609173485179
1106	22	0.07598190222298484
1106	23	0.010476006503843394
1106	24	0.14297345150584562
1106	25	0.46589771327195706
1107	1	0.07630479868470985
1107	2	-0.019779006142101707
1107	3	-0.15144759015952047
1107	4	0.02666931211094692
1107	5	0.13679559345712278
1107	6	0.07352387990101761
1107	7	-0.03789460507272935
1107	8	-0.17759514020621836
1107	9	0.6057846605650496
1107	10	0.4195934540843069
1107	11	0.4007594591432877
1107	12	0.3552300864310922
1107	13	0.1269001081727481
1107	14	0.22482547305971678
1107	15	0.17457923589144286
1107	16	0.0643586865346748
1107	17	-0.19353232080518978
1107	18	-0.059502443111448144
1107	19	0.25545094134695745
1107	20	0.10776296950403008
1107	21	-0.013292872629037333
1107	22	0.13392845880701287
1107	23	0.3402629681618663
1107	24	0.5671826121712584
1107	25	0.24000517870294402
1108	1	-0.09552020386390225
1108	2	0.005590694460892109
1108	3	0.07252166827866091
1108	4	-0.09454151347131301
1108	5	0.16227667212015717
1108	6	0.060784938205607375
1108	7	-0.31423224492295176
1108	8	0.025862448641365887
1108	9	0.32598760599741183
1108	10	0.2344814445776109
1108	11	0.28192439453032136
1108	12	0.2967413893261579
1108	13	-0.12427339358693246
1108	14	0.008954860282585742
1108	15	-0.21973515759263051
1108	16	-0.7421648420805056
1108	17	-0.7303442912078353
1108	18	-0.7994730161759165
1108	19	-0.6464529844253448
1108	20	-0.09602236297926225
1108	21	-0.28515743569077756
1108	22	-0.07117636894499668
1108	23	-0.5797071788663004
1108	24	-0.28045068013072694
1108	25	-0.4456087928530508
1109	1	0.24889429344976222
1109	2	0.24903514220347844
1109	3	0.14128179769775784
1109	4	0.1521740316621134
1109	5	0.2526070470199784
1109	6	0.4732685381937358
1109	7	0.5519311912134768
1109	8	0.464541542707682
1109	9	1.3063043265887018
1109	10	0.6789662912634782
1109	11	0.7756727990540497
1109	12	0.850204537534772
1109	13	0.8952018060331213
1109	14	0.45260352898750256
1109	15	0.20993743583549218
1109	16	0.6125927939431222
1109	17	0.5716973247498913
1109	18	0.4201722686351488
1109	19	0.6582809049747596
1109	20	0.9474393010653799
1109	21	0.9231111386213087
1109	22	0.6119756984954208
1109	23	1.0110512289225895
1109	24	0.7467418753028028
1109	25	0.7921486353876261
1110	1	0.11476810059973985
1110	2	0.11264716328309632
1110	3	0.06310489981996525
1110	4	0.2226625660589221
1110	5	0.1731283402830428
1110	6	0.4093004424808697
1110	7	0.37950276221475576
1110	8	0.24236806314636183
1110	9	-3.2927399690956776
1110	10	-1.245792290135185
1110	11	-0.18769281837936092
1110	12	0.3010349035794988
1110	13	-0.0853907021566016
1110	14	0.2540734050735267
1110	15	0.35334435792636043
1110	16	-0.0022384266807193853
1110	17	0.048347374600339776
1110	18	0.19730515568827656
1110	19	0.015805474124448438
1110	20	0.5180702653154867
1110	21	0.22847219829941423
1110	22	0.42260808704405406
1110	23	0.6942900837865693
1110	24	0.7768643649766389
1110	25	0.7827012632184136
1111	1	0.17337700895181385
1111	2	0.19802058025551972
1111	3	0.15810351898593922
1111	4	-0.009556236955241104
1111	5	0.2550165321795248
1111	6	0.16444543005336953
1111	7	0.3771869062949236
1111	8	0.4062998893979888
1111	9	1.1252645284779987
1111	10	0.6548748499602866
1111	11	0.8042454780659725
1111	12	0.8435184322022096
1111	13	0.5877986299142606
1111	14	0.3665865229967551
1111	15	0.48947920623187713
1111	16	0.4026071171706249
1111	17	0.43375748230539557
1111	18	0.4064170821490052
1111	19	0.46850007309079444
1111	20	0.6801430944471463
1111	21	0.6751617473584801
1111	22	0.6148288626782517
1111	23	0.9326587270185129
1111	24	0.7894536260331719
1111	25	1.0137396504042755
1112	1	-0.262120968508583
1112	2	-0.2980501583315629
1112	3	-0.2194778400416304
1112	4	-0.17981860187644827
1112	5	-0.08122542889461215
1112	6	-0.35675499673547506
1112	7	-0.18989309466013538
1112	8	-0.637092233204335
1112	9	-0.06047976688758183
1112	10	-0.1616291739016873
1112	11	0.02342145287325792
1112	12	-0.6111050683195032
1112	13	-0.47429772641391565
1112	14	-1.2041161079511866
1112	15	-0.544446495212604
1112	16	-0.5979441913554098
1112	17	-0.6827557526637236
1112	18	-0.7314747760583344
1112	19	-0.2637628877234093
1112	20	-0.2760300277458896
1112	21	-0.5258638766063299
1112	22	-0.19157046914305198
1112	23	-0.0634710520495787
1112	24	-0.03281885367947979
1112	25	-0.07743030957266939
1113	1	-2.65019883388225
1113	2	-2.5171705016783044
1113	3	-2.7082377392369494
1113	4	-2.662014455169192
1113	5	-2.638942011421225
1113	6	-2.569367388757499
1113	7	-3.1283559267740735
1113	8	-2.9753807691173364
1113	9	-12.149366472505303
1113	10	-5.421869130167519
1113	11	-3.971420569001886
1113	12	-14.001240134260044
1113	13	-6.142020677581243
1113	14	-4.941041578298773
1113	15	-4.379254716564638
1113	16	-4.188087469977834
1113	17	-4.187863463511533
1113	18	-4.495061944799431
1113	19	-5.265733713532537
1113	20	-15.925255165566998
1113	21	-18.065197775823222
1113	22	-13.041982459310056
1113	23	-18.964854932515628
1113	24	-20.068862615292552
1113	25	-16.89869936933932
1114	1	-0.3539583084529454
1114	2	-0.294169471919691
1114	3	-0.2722263062210999
1114	4	-0.7415989238253005
1114	5	-0.3950012017922333
1114	6	-0.2639116574684479
1114	7	-0.40758201205280287
1114	8	-0.2614981513314767
1114	9	0.4349436769273467
1114	10	-0.3411750078345932
1114	11	-0.397042434965563
1114	12	-0.4001866745149627
1114	13	-0.3892165426319565
1114	14	-0.5038264785379667
1114	15	-0.36628893475452884
1114	16	-0.5009970792471137
1114	17	-0.5502469418538709
1114	18	-0.5228697055086826
1114	19	-0.779436519293192
1114	20	-1.0139305588710457
1114	21	-1.1648008246274486
1114	22	-1.4718489243887483
1114	23	-1.0095555260279347
1114	24	-0.6191987521191109
1114	25	-0.9378739410183492
1115	1	-0.1403609320018937
1115	2	-0.0678200273954559
1115	3	-0.06136347308052592
1115	4	0.003364124248740552
1115	5	-0.2646999925710105
1115	6	-0.2229209099384235
1115	7	-0.533699514893808
1115	8	-0.32791114359336504
1115	9	0.26318903368414226
1115	10	-0.1670133869389261
1115	11	0.04140640305826082
1115	12	-0.044581396172347354
1115	13	-0.17241059756723587
1115	14	-0.2463088466864889
1115	15	-0.21133085056007417
1115	16	-0.041454272714950946
1115	17	-0.07017868221021217
1115	18	-0.13276955860808926
1115	19	-0.17004061335997278
1115	20	-0.49453552224668584
1115	21	0.036552525487330456
1115	22	-0.5374848931137848
1115	23	-0.16493188637031864
1115	24	0.21923956600761585
1115	25	0.3136908149326345
1116	1	-0.6313293826581976
1116	2	-0.4932777730884048
1116	3	-0.48154693330738496
1116	4	-0.33559074002315015
1116	5	-1.2104582138055129
1116	6	-1.0253032476386785
1116	7	-2.0942008363117566
1116	8	-1.3123636705752055
1116	9	-0.332148360724581
1116	10	-1.4501924090594827
1116	11	-1.1197460356681823
1116	12	-1.986988566636768
1116	13	-1.694215124833807
1116	14	-1.1348007471575698
1116	15	-2.2675549070628342
1116	16	-2.4984750860279448
1116	17	-3.1236023038907597
1116	18	-2.3467479496090533
1116	19	-1.8436569400654044
1116	20	-2.280850536703557
1116	21	-1.4877407828473501
1116	22	-1.3541551189522885
1116	23	-1.458816173471213
1116	24	-2.0787024786099195
1116	25	-1.5056504487181428
1201	1	0.8075508716215651
1201	2	0.8686740254119094
1201	3	0.4582238530080538
1201	4	0.9076623573168021
1201	5	1.1497367579581308
1201	6	1.2057493215356914
1201	7	1.3783988146030666
1201	8	1.3580976168557122
1201	9	2.088769832837869
1201	10	1.7517014923316694
1201	11	1.7617789447758625
1201	12	1.8862430791137257
1201	13	1.685559193770544
1201	14	1.7084627150913647
1201	15	1.7171638615972356
1201	16	1.6373155808697817
1201	17	1.6072548183443445
1201	18	1.6448481416548404
1201	19	1.7325832711197577
1201	20	1.8842983548479708
1201	21	1.9424824922679633
1201	22	1.9141284453648169
1201	23	2.319328045032101
1201	24	2.2620633558575847
1201	25	2.3700901975004314
1202	1	0.7363166306301494
1202	2	0.8081190717762716
1202	3	0.7866182705242117
1202	4	0.9011618680835625
1202	5	0.9194044998028019
1202	6	0.8454787981120546
1202	7	0.9857941476412648
1202	8	0.9721195431598169
1202	9	1.6453925719858873
1202	10	1.2853817495482431
1202	11	1.350528952920527
1202	12	1.3515557722422216
1202	13	1.363775264593862
1202	14	1.4054678564969194
1202	15	1.410299671491245
1202	16	1.3171388249994394
1202	17	1.39709275115041
1202	18	1.3799731828853012
1202	19	1.4075293349835363
1202	20	1.578306135863258
1202	21	1.6357870965434884
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2211	4	-0.13835119097972637
2211	5	-0.7735599322956119
2211	6	-0.07665303788738242
2211	7	0.0922005375706334
2211	8	0.05774210354750764
2211	9	0.5347739170048413
2211	10	-0.11400406631323534
2211	11	0.23548936917142527
2211	12	0.28232705183811646
2211	13	0.2737945828789208
2211	14	0.21229043932750358
2211	15	0.15249427045109343
2211	16	0.109696067253374
2211	17	0.07183108890517786
2211	18	0.054217422301405804
2211	19	-0.17034379787103404
2211	20	0.2426329368004687
2211	21	0.4371214463945634
2211	22	0.2517756858241853
2211	23	0.6719665878352856
2211	24	0.656141809742941
2211	25	0.8395414463906619
2212	1	0.13290379084314097
2212	2	0.09699996707491465
2212	3	0.13246400040842943
2212	4	0.2900451594232436
2212	5	0.013615139907528828
2212	6	0.11364729293767795
2212	7	0.11638138287283084
2212	8	0.04051840775287374
2212	9	0.615577297865606
2212	10	0.15093200176141153
2212	11	0.3401490976786121
2212	12	0.20459591381465003
2212	13	-0.04096462654924693
2212	14	0.00871769330653649
2212	15	0.2224680728074091
2212	16	0.24383076939007964
2212	17	-0.03894546078994665
2212	18	0.07863320734490742
2212	19	0.2870897916661832
2212	20	0.3980313396634827
2212	21	0.5065652993000356
2212	22	0.14931520585888336
2212	23	0.48547960591695016
2212	24	0.6493691161959276
2212	25	0.6689563865211454
2213	1	-0.08101298455423134
2213	2	-0.06045396701188466
2213	3	-0.149288894243913
2213	4	-0.11849484620130113
2213	5	-0.13679271739970744
2213	6	0.17890412454984528
2213	7	0.04959806746791173
2213	8	0.08122551638199482
2213	9	0.6678866959555734
2213	10	0.1714866162434987
2213	11	0.3453214603922419
2213	12	0.2851350578158218
2213	13	-0.07501183620879459
2213	14	0.13717373219611773
2213	15	0.1731418223519512
2213	16	-0.09445756478145029
2213	17	-0.0923962813587238
2213	18	-0.19890174696586627
2213	19	-0.081783783512833
2213	20	0.25053797846587145
2213	21	0.07206055475005257
2213	22	0.2251612495693962
2213	23	-0.11492954574676162
2213	24	0.033284775356889895
2213	25	0.34483342727794764
2214	1	-0.7799113925783772
2214	2	-0.6000462169643228
2214	3	-0.5206172839310419
2214	4	-0.3675172920758669
2214	5	-0.6601283833418172
2214	6	-0.5079071710767984
2214	7	-0.517805916267772
2214	8	-0.5248056749457568
2214	9	-10.356931543275458
2214	10	-3.7198788690874807
2214	11	-1.5247784053981341
2214	12	-0.9490569906392174
2214	13	-0.634606700359889
2214	14	-0.7025480319199744
2214	15	-0.7135029778899238
2214	16	-0.8258191195369307
2214	17	-0.7596288938580429
2214	18	-1.024068578660696
2214	19	-1.2330182424445795
2214	20	-1.0023789121283144
2214	21	-1.4739848023182027
2214	22	-1.2638836499625512
2214	23	-1.6540818351369033
2214	24	-2.0131489231894086
2214	25	-9.7686969887327
2215	1	-0.5118318427483857
2215	2	-0.21417466657243978
2215	3	-0.11584043112140208
2215	4	-0.2784610294466958
2215	5	-0.36394464621492517
2215	6	-0.2619553407312907
2215	7	-0.3641569488606855
2215	8	-0.3903351754524458
2215	9	0.43738277663182856
2215	10	0.38584117223852843
2215	11	0.45096075540970304
2215	12	0.5158389825144383
2215	13	0.2833648450060906
2215	14	0.09123325777865432
2215	15	-0.3318761122841991
2215	16	-0.009923253420403455
2215	17	-0.06965659103085642
2215	18	-0.0048872718445309715
2215	19	-0.04326826773183967
2215	20	0.18589483334494616
2215	21	0.013853182153631849
2215	22	-0.0887779954788428
2215	23	0.30004402581281653
2215	24	0.4897385033960475
2215	25	0.581718353200479
2216	1	-0.4926595270387857
2216	2	-0.47832040300907863
2216	3	-0.5004978371711601
2216	4	-0.29094616036901044
2216	5	-1.0167074039498551
2216	6	-1.4797740987827162
2216	7	-0.9541669475698065
2216	8	-1.784445011975695
2216	9	-0.4781464340574715
2216	10	-0.9176535529988641
2216	11	-0.48020130341484446
2216	12	-1.562259286666798
2216	13	-1.1981267857909152
2216	14	-1.3390964589844074
2216	15	-1.0305629328517227
2216	16	-2.1235041943268733
2216	17	-2.463799566249431
2216	18	-1.9103075999971502
2216	19	-3.241664079571759
2216	20	-2.1454765702653944
2216	21	-2.3222612354628573
2216	22	-2.0748650966138555
2216	23	-1.6995756439300038
2216	24	-1.8931249266363395
2216	25	-1.8585064250404741
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	127911.0
3	2.0
4	1764.0
5	18799.0
6	25819.0
7	20305.0
8	16112.0
9	12920.0
10	11409.0
11	10258.0
12	9335.0
13	8487.0
14	8539.0
15	8715.0
16	9047.0
17	9696.0
18	10957.0
19	13629.0
20	18167.0
21	24357.0
22	29799.0
23	35772.0
24	40999.0
25	47024.0
26	51940.0
27	57621.0
28	63853.0
29	73505.0
30	90158.0
31	110437.0
32	135928.0
33	168572.0
34	209605.0
35	259423.0
36	338636.0
37	490523.0
38	1153104.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.691623902139824	18.060184405314487	12.417201240043664	24.830990452502025
2	16.92043023460383	20.253284574485946	36.997892876472065	25.828392314438158
3	19.39520031199664	24.462003208601825	28.655048981807447	27.487747497594086
4	12.437146921488447	15.712920444118778	34.260783332008224	37.58914930238455
5	13.266211707718925	38.36553861960916	33.08860414621846	15.279645526453455
6	33.796477742179064	35.5368957057423	16.15988421210696	14.506742339971671
7	29.54266795473424	32.18858835172841	19.557072748236877	18.711670945300472
8	27.987652198209624	34.31063479952285	18.2732152644192	19.428497737848325
9	26.97093250543076	14.556155465486967	18.516868604499635	39.95604342458263
10	15.708557568631518	30.159874875158632	30.66547402909889	23.466093527110964
11	35.90474790691576	22.48861462664117	20.97781071408707	20.628826752356005
12	23.714512884015654	26.50236513620015	28.435813614911236	21.347308364872962
13	29.47997792112038	20.721538860305426	25.52869112802393	24.269792090550265
14	22.12987428808419	20.418885716611104	27.250027331013083	30.20121266429162
15	24.372236498884327	28.211060370537805	23.535084192014793	23.881618938563076
16	23.47986998038854	28.18949490749359	24.70039537200667	23.6302397401112
17	22.97434437260427	27.405748945006003	26.473324970384954	23.14658171200477
18	22.067091031391097	25.833077238821208	29.110531824130764	22.98929990565693
19	23.499087303239637	25.006171952338292	28.923098414097037	22.571642330325037
20	23.325847465595007	24.898927353951706	29.257609662142016	22.517615518311274
21	24.019116289327417	24.29816189319065	29.067214586015645	22.615507231466285
22	23.615229972127846	24.51742170761531	28.949620599176086	22.91772772108076
23	22.805588699682335	24.560908220107564	29.524110628618583	23.109392451591518
24	23.271896147096832	24.64902853462144	28.958835912294806	23.120239405986922
25	22.621644012200097	24.608986264270353	29.57447821140542	23.194891512124137
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2446.0
1	2446.0
2	3170.5
3	3895.0
4	3895.0
5	3895.0
6	9817.5
7	15740.0
8	15740.0
9	15740.0
10	17716.5
11	19693.0
12	19693.0
13	19693.0
14	20195.5
15	20698.0
16	20698.0
17	20698.0
18	30783.0
19	40868.0
20	40868.0
21	40868.0
22	61340.5
23	81813.0
24	81813.0
25	81813.0
26	117604.0
27	153395.0
28	153395.0
29	153395.0
30	179199.5
31	205004.0
32	205004.0
33	205004.0
34	233791.5
35	262579.0
36	262579.0
37	262579.0
38	289130.0
39	315681.0
40	315681.0
41	315681.0
42	347801.5
43	379922.0
44	379922.0
45	379922.0
46	416699.5
47	453477.0
48	453477.0
49	453477.0
50	461738.0
51	469999.0
52	469999.0
53	469999.0
54	458078.5
55	446158.0
56	446158.0
57	446158.0
58	395820.0
59	345482.0
60	345482.0
61	345482.0
62	292908.0
63	240334.0
64	240334.0
65	240334.0
66	188655.0
67	136976.0
68	136976.0
69	136976.0
70	103636.0
71	70296.0
72	70296.0
73	70296.0
74	50354.5
75	30413.0
76	30413.0
77	30413.0
78	23965.5
79	17518.0
80	17518.0
81	17518.0
82	12273.0
83	7028.0
84	7028.0
85	7028.0
86	4835.0
87	2642.0
88	2642.0
89	2642.0
90	1809.5
91	977.0
92	977.0
93	977.0
94	534.0
95	91.0
96	91.0
97	91.0
98	46.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.026859142865661042
2	0.0011280840003577638
3	2.685914286566104E-4
4	0.004270603715640106
5	0.03561522343986655
6	0.012301487432472757
7	0.020278652863574085
8	0.02704715686572067
9	0.034164829725120846
10	0.0377102365833881
11	0.03937550344105909
12	0.00797716543110133
13	0.0030082240009540366
14	0.0067147857164152605
15	0.00719825028799716
16	0.008165179431160956
17	0.009588714003040993
18	0.014584514576053946
19	0.01724356971975439
20	0.0149605425761732
21	0.011764304575159536
22	0.011146544289249332
23	0.008836658002802483
24	0.006607349144952616
25	0.11119685146383672
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3723127.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.5292726657319
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.82945774660456	28.833536685326504
2	9.220002054438975	6.920399421592973
3	4.27506960226897	4.813207583056027
4	2.42391788896132	3.6387150149669854
5	1.514020226209154	2.8410038945418217
6	1.06923102109709	2.4076477520046975
7	0.7591882685953656	1.9944248475718271
8	0.5593077035297171	1.6792329047848875
9	0.42567002228603007	1.4377577698800539
>10	2.5224629870276356	17.36095263741663
>50	0.22446989870966313	5.878420782520854
>100	0.15775676183283152	11.467871889147826
>500	0.013312370330500714	3.4072963877585383
>1k	0.0057152584643813216	3.7149057088374953
>5k	0.0	0.0
>10k+	4.1818964373521874E-4	3.604626720593006
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	40218	1.0802210077711558	No Hit
TATCAACGCAGAGTACTTTTTTTTT	29960	0.8046999202552049	No Hit
GGTATCAACGCAGAGTACTTTTTTT	28281	0.75960341938376	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	16519	0.44368618099785473	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	11998	0.3222559961022012	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	10830	0.2908845172351091	No Hit
GTGGTATCAACGCAGAGTACATGGG	4968	0.13343622175660408	No Hit
GTGGTATCAACGCAGAGTACTTTTT	4620	0.12408924003935401	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	4559	0.12245083232454868	No Hit
TGCATGCACTGCCTCAGTGACCAGT	4176	0.11216378060700052	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	4160	0.11173403432114995	No Hit
GTCCCAGGGTGTGCTTGTCAAAGAG	3824	0.10270936231828784	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	2.685914286566104E-5	0.0	0.0	0.0	0.0
7	1.342957143283052E-4	0.0	0.0	0.0	0.0
8	1.6115485719396625E-4	0.0	0.0	0.0	0.0
9	1.6115485719396625E-4	0.0	0.0	0.0	0.0
10	1.6115485719396625E-4	0.0	0.0	0.0	0.0
11	1.880140000596273E-4	0.0	0.0	2.685914286566104E-5	2.685914286566104E-5
12	2.1487314292528833E-4	0.0	0.0	2.685914286566104E-5	2.685914286566104E-5
13	2.1487314292528833E-4	0.0	0.0	5.371828573132208E-5	2.685914286566104E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAACGC	40	2.75887E-4	16.6278	3
TATCACG	115	0.0	16.5223	2
CGTTGCG	35	0.0021769104	16.279047	12
TATAACG	275	0.0	15.545982	2
TAACGCA	280	0.0	14.931686	4
ATAACGC	300	0.0	14.885839	3
AGGACCG	355	0.0	14.188918	5
GGTATCA	6845	0.0	13.478348	1
TCGACCG	50	0.0015039549	13.295627	18
GTGGTAT	2045	0.0	12.916426	1
GGACCGA	275	0.0	12.784314	6
TTGACCG	530	0.0	12.184671	18
TGGTATC	2010	0.0	12.147179	2
TGACCGA	550	0.0	11.579656	19
ACCGAGT	345	0.0	11.291756	8
GTATAAC	480	0.0	11.282998	1
TACAACG	135	4.7348294E-9	11.259642	2
CGCGAAT	110	4.995891E-7	11.223582	18
CCGTAAG	85	5.3188538E-5	11.178202	1
CGACCGT	60	0.0058521368	11.08997	19
>>END_MODULE