FastQCFastQC Report
Thu 26 May 2016
SRR1364278_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364278_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1757878
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT79450.4519653809877591No Hit
TATCAACGCAGAGTACTTTTTTTTT50800.28898478734019084No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN46520.2646372501390882No Hit
GGTATCAACGCAGAGTACTTTTTTT36220.2060438779027896No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA33500.19057067669087388No Hit
GTACTTTTTTTTTTTTTTTTTTTTT25970.14773493951229835No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19470.11075853955735268No Hit
GTGGTATCAACGCAGAGTACATGGG17640.10034826080080643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCA802.1827873E-1117.83250218
AAGACGT350.002164859316.2918875
CGTTCGC902.2519089E-914.79437215
TTCGCGC954.825779E-914.0157217
ACGGCCC1700.013.42682515
CGGCCCC2000.012.83940216
CGCGCAG1051.9630534E-812.6808919
GTTCGCG1051.9630534E-812.6808916
TAACGCA905.3796066E-712.671834
CGCACCT751.4730851E-512.6714685
GGTATCA22500.012.23230651
TCGTTCG1103.7540303E-812.10483214
CCGATCA1103.7554855E-812.104485519
TCCCGAT1052.6810994E-711.77511217
CCCGATC1052.6810994E-711.77511218
TGCACGG1800.011.59397512
CTTCGCG1156.965456E-811.57820415
ATTTGCG1900.011.51291416
TTGACCG2400.011.49205718
TTGCGCG1751.8189894E-1211.41280118
Produced by FastQC (version 0.11.5)