##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364278_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1757878
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.05337173569497	30.0	26.0	31.0	7.0	34.0
2	26.00945287443156	30.0	26.0	31.0	7.0	34.0
3	25.386568920027443	30.0	24.0	31.0	7.0	34.0
4	29.234898553824554	35.0	28.0	37.0	7.0	37.0
5	29.76050783956566	35.0	30.0	37.0	7.0	37.0
6	29.956022545364355	35.0	30.0	37.0	2.0	37.0
7	29.634223194101068	35.0	30.0	37.0	2.0	37.0
8	29.127731276004365	35.0	28.0	37.0	2.0	37.0
9	30.165140584272628	35.0	28.0	39.0	2.0	39.0
10	29.337147970450737	35.0	27.0	39.0	2.0	39.0
11	30.038023116507517	35.0	28.0	39.0	2.0	39.0
12	29.500290691390415	35.0	27.0	39.0	2.0	39.0
13	29.170202368992616	35.0	27.0	39.0	2.0	39.0
14	29.34112037354128	36.0	25.0	40.0	2.0	41.0
15	30.122988626059374	36.0	27.0	40.0	2.0	41.0
16	30.457399774045754	36.0	27.0	40.0	2.0	41.0
17	30.617418273623084	37.0	28.0	40.0	2.0	41.0
18	30.42441341207979	37.0	27.0	40.0	2.0	41.0
19	30.400815642496237	37.0	27.0	40.0	2.0	41.0
20	30.27252687615409	37.0	27.0	40.0	2.0	41.0
21	30.19302761625096	37.0	27.0	40.0	2.0	41.0
22	30.266669814401226	37.0	27.0	40.0	2.0	41.0
23	30.273292572067003	37.0	28.0	40.0	2.0	41.0
24	30.158773817068077	37.0	28.0	40.0	2.0	41.0
25	29.949279187747955	37.0	27.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.09072881887924922
1101	2	0.3015608483449306
1101	3	0.9287219565294471
1101	4	0.2723524278305973
1101	5	-0.37064147704801087
1101	6	-0.49750788301681226
1101	7	-0.29569465124160743
1101	8	0.0300705439411324
1101	9	-0.1744774289722244
1101	10	0.11071767309310943
1101	11	-0.41240054739291665
1101	12	-0.11141086356749952
1101	13	-0.19769868689838432
1101	14	0.20411542470444388
1101	15	-0.45696835373719225
1101	16	-1.0319349149190522
1101	17	-1.832809876429927
1101	18	-7.633740977157572
1101	19	-8.918154727183385
1101	20	-10.218770498096596
1101	21	-11.727647629721176
1101	22	-12.161788899773669
1101	23	-13.589764387346932
1101	24	-13.060706984598276
1101	25	-13.558430351468957
1102	1	1.4497220167410276
1102	2	1.6019973466479662
1102	3	2.3532129861799156
1102	4	1.6200471265420475
1102	5	1.0172427199997927
1102	6	1.0682122439818222
1102	7	1.1820936780218645
1102	8	1.463334618274363
1102	9	1.4623076682045806
1102	10	-1.9770201518410815
1102	11	0.12988290518013912
1102	12	1.0726931248940943
1102	13	1.6547463224075472
1102	14	2.2167567392450245
1102	15	1.869053514018578
1102	16	1.367734498307275
1102	17	1.1598524776144643
1102	18	1.371612196142248
1102	19	0.9987494669131749
1102	20	1.5280481099641037
1102	21	1.5757132168094472
1102	22	1.3727008922589299
1102	23	1.4123819276458534
1102	24	1.5870043743537465
1102	25	1.693876139836778
1103	1	-0.7801582889841079
1103	2	-0.3032086332587802
1103	3	-2.653997512305878
1103	4	-0.21153485682573603
1103	5	-3.8752872035689236
1103	6	-7.351265715927809
1103	7	-8.328337509787584
1103	8	-2.3093375924644697
1103	9	-8.34839751854733
1103	10	-4.864997847365768
1103	11	-9.116120075159234
1103	12	-3.0709400049429476
1103	13	0.041541496180560245
1103	14	0.8735322043221778
1103	15	0.05083018084547675
1103	16	-0.2163447210360907
1103	17	-0.22477954661281174
1103	18	-0.1535908995650992
1103	19	-5.602711860779944
1103	20	-1.5066680367455056
1103	21	-8.84051521178258
1103	22	-3.3492238301368786
1103	23	-1.6187289698208573
1103	24	-5.728950055295822
1103	25	-3.213968882515765
1104	1	1.2499334687455068
1104	2	1.2190961298058056
1104	3	2.106240336469515
1104	4	1.4893977089714419
1104	5	1.260314233453613
1104	6	1.0228308755933853
1104	7	1.0457122426679533
1104	8	1.4944840069022653
1104	9	1.4997314949751086
1104	10	1.7673576424069246
1104	11	1.2980051818701384
1104	12	1.4577934198826945
1104	13	1.8401124362874484
1104	14	2.118945488817978
1104	15	1.259306178166284
1104	16	0.3717970327122053
1104	17	0.2113483615076781
1104	18	0.2770381022475732
1104	19	0.8431257856506846
1104	20	-9.376350046328778
1104	21	-3.774507258509235
1104	22	-1.4698461339631628
1104	23	-0.42015943488593877
1104	24	-0.0154716457631352
1104	25	0.5646205121829873
1105	1	0.6005602053034629
1105	2	0.5664587161165926
1105	3	1.4834550459104072
1105	4	0.613670683792197
1105	5	0.31829954088123813
1105	6	0.2819158684093672
1105	7	0.6506230904783799
1105	8	0.871057666754524
1105	9	0.6814313512947408
1105	10	1.2902132261046901
1105	11	0.8919850684522608
1105	12	1.1033933833953498
1105	13	1.1383130281983043
1105	14	1.774819320541198
1105	15	1.11656435154854
1105	16	0.6160001406911313
1105	17	-0.028431229279451742
1105	18	0.315711475678782
1105	19	0.20086216781616528
1105	20	0.23455462659497783
1105	21	0.20284973322862854
1105	22	0.23853497632005727
1105	23	0.29274053639632314
1105	24	0.24424958361274918
1105	25	0.2291398040626973
1106	1	0.49979115124702034
1106	2	0.7705484390898434
1106	3	1.2342485452359533
1106	4	0.8429589435835574
1106	5	0.14049466714252645
1106	6	-0.11526956810067546
1106	7	0.3461273993252121
1106	8	0.3910765161186518
1106	9	0.485756809128123
1106	10	-3.0859632981903715
1106	11	-0.6989803135124326
1106	12	0.04956077752966337
1106	13	0.9469875055702346
1106	14	0.028765140400182077
1106	15	0.7034171556859725
1106	16	0.29833687280043364
1106	17	-6.4541523647180234
1106	18	-2.3961670401694093
1106	19	-0.9831717139158549
1106	20	-0.23817399557540853
1106	21	0.15977610879042814
1106	22	0.1652314485437536
1106	23	-0.36213573519945186
1106	24	0.06430141936410294
1106	25	0.1629686400555599
1107	1	0.5487483926750123
1107	2	0.7934830100629533
1107	3	1.3681955220184427
1107	4	0.8328075915326885
1107	5	0.40432370344231217
1107	6	0.5395526976507057
1107	7	0.3876798607813221
1107	8	0.5125074488778694
1107	9	0.6180633249721481
1107	10	-2.570468801099178
1107	11	-0.4527184679938756
1107	12	0.03388439915541852
1107	13	0.8306810132554823
1107	14	1.2473823250049776
1107	15	1.138725065639445
1107	16	-5.532727532703063
1107	17	-2.022744902408977
1107	18	-0.3784292621563381
1107	19	0.354574181696524
1107	20	0.7480211444531797
1107	21	0.9856385448937459
1107	22	0.5396413637795447
1107	23	0.17149136111174457
1107	24	0.5257003523115564
1107	25	0.8414531195586221
1108	1	-0.8156164799249872
1108	2	-3.4583159955249414
1108	3	-0.46261711209253775
1108	4	-1.143043262096068
1108	5	-1.3162824033792866
1108	6	-0.8892474633548666
1108	7	-0.5014060754593146
1108	8	-0.19000942147500055
1108	9	-0.9483570231203515
1108	10	-3.9328671952906227
1108	11	-1.9797344401711854
1108	12	-6.49831985204802
1108	13	-2.169108801115133
1108	14	-6.489153728949262
1108	15	-3.250016272347736
1108	16	-8.264247848073207
1108	17	-3.925014617627962
1108	18	-2.4561460622381937
1108	19	-1.1070231422132863
1108	20	-1.0889626459152488
1108	21	-0.4920323087543146
1108	22	-0.634252374474432
1108	23	-0.6366506654927839
1108	24	-0.38906008433400885
1108	25	-0.693118676087856
1109	1	-8.803725458449403
1109	2	-6.767529175873751
1109	3	-5.983212554539993
1109	4	-5.566417857897964
1109	5	-3.005849079821271
1109	6	-2.177979702915529
1109	7	-1.6955802358650267
1109	8	-1.1133504397860854
1109	9	-6.2298800063089
1109	10	-2.3248523931731633
1109	11	-1.9687373288016552
1109	12	-6.8282284826226665
1109	13	-8.634764704786868
1109	14	-8.771575091190478
1109	15	-4.636501525548589
1109	16	-3.084671803752851
1109	17	-2.7079416955311224
1109	18	-2.1037832697697922
1109	19	-1.789347347139369
1109	20	-1.7225471904136391
1109	21	-1.3563317784232112
1109	22	-1.5484082580962983
1109	23	-1.8814198238669384
1109	24	-1.643203401761113
1109	25	-1.5621638047186366
1110	1	-2.6464379227093247
1110	2	-2.4315212084343507
1110	3	-1.6744210175245193
1110	4	-2.5835035837880618
1110	5	-5.726813025165168
1110	6	-4.24912878968717
1110	7	-8.975429427686162
1110	8	-10.139780303829077
1110	9	-11.482161535153857
1110	10	-9.351684496184298
1110	11	-12.302696143137354
1110	12	-7.003699093912026
1110	13	-5.184169519223154
1110	14	-4.643006421371378
1110	15	-10.236156200251017
1110	16	-11.923635296444463
1110	17	-12.338774812976833
1110	18	-9.284352743355115
1110	19	-12.631491979508848
1110	20	-13.500615190743876
1110	21	-14.271877490135898
1110	22	-15.466352220312643
1110	23	-16.110901010328362
1110	24	-16.54347494568499
1110	25	-17.04679296197877
1111	1	-0.5403729942309319
1111	2	0.1892874871175927
1111	3	-2.6866894800116974
1111	4	-3.0156531509699604
1111	5	-0.930613583045222
1111	6	-0.337466042012629
1111	7	0.16699712002756684
1111	8	0.4296521338992889
1111	9	-3.990028335527313
1111	10	-3.7292404859027073
1111	11	-0.99294448044731
1111	12	-5.757854378211849
1111	13	-1.0948395516124734
1111	14	0.3788016171730604
1111	15	0.31813624877326774
1111	16	0.16297442957188224
1111	17	0.2773714345882041
1111	18	0.5963845458549812
1111	19	0.5964896242810482
1111	20	0.7540328491837371
1111	21	0.8776522308088097
1111	22	0.4204984084550638
1111	23	0.33666518029602344
1111	24	0.5379680502692281
1111	25	0.688082898182369
1112	1	-0.2956730556539888
1112	2	0.21494856343235114
1112	3	-2.709196911799708
1112	4	-0.582357560422178
1112	5	-0.5659529843537392
1112	6	-0.37577032369685526
1112	7	0.08437607818606097
1112	8	-3.5477065893047133
1112	9	-0.9382629625617973
1112	10	-3.473223361777407
1112	11	-1.0458471593061773
1112	12	0.225570060128792
1112	13	0.6977593216787454
1112	14	1.4323173965703724
1112	15	0.7484621375116554
1112	16	0.5455398742562281
1112	17	0.3163150584681631
1112	18	0.4227175090428297
1112	19	0.6240806552090383
1112	20	0.5717248991920485
1112	21	0.7458785559371677
1112	22	0.5352674894044256
1112	23	0.10168570403430266
1112	24	0.40179444048693824
1112	25	0.3117098568270258
1113	1	-1.119185776110541
1113	2	-0.6180878921174404
1113	3	-3.1106858896483978
1113	4	-1.3720135889849665
1113	5	-1.3980011164283823
1113	6	-1.0653784390108818
1113	7	-0.898501397958011
1113	8	-9.022603834322968
1113	9	-8.755272827160276
1113	10	-3.028251156872223
1113	11	-8.930376229783139
1113	12	-7.211244267916449
1113	13	-2.4836413202505803
1113	14	-1.1293599398617928
1113	15	-7.447700233695187
1113	16	-3.180898319756963
1113	17	-1.812590216978915
1113	18	-1.0701889855769977
1113	19	-1.000064255616408
1113	20	-6.989950032560127
1113	21	-2.5067547189424033
1113	22	-1.7701954024757534
1113	23	-1.695453087481745
1113	24	-7.6561409132985965
1113	25	-4.302531980637049
1114	1	-3.945638516698917
1114	2	-3.8687937564345667
1114	3	-0.4366093074440265
1114	4	-0.043814143536600625
1114	5	-0.027518014319181106
1114	6	-0.25298987141750473
1114	7	-0.1708364659249888
1114	8	0.3993279946955077
1114	9	0.2512124332822303
1114	10	-3.2832404919534675
1114	11	-0.9040784835997542
1114	12	-4.176879328519984
1114	13	-0.8901491538657744
1114	14	-5.731729881569894
1114	15	-1.673228560612671
1114	16	-0.5546835028229786
1114	17	-0.2940971150749192
1114	18	-0.024172305571639185
1114	19	0.14180149556928612
1114	20	0.42163133286332766
1114	21	0.4249517766992845
1114	22	0.4256226456811518
1114	23	-0.05617359418308254
1114	24	0.11248467019377273
1114	25	0.2623990866877399
1115	1	1.1745052707049162
1115	2	1.3433125141233155
1115	3	2.1273523732446122
1115	4	0.8883764705823438
1115	5	0.834619380886565
1115	6	0.2995086226365231
1115	7	-1.8970329916097022
1115	8	0.5102394598936009
1115	9	0.8308706232463301
1115	10	1.4271216337964638
1115	11	0.612376821300046
1115	12	1.4275907108304615
1115	13	1.5558064416366086
1115	14	2.337815709157926
1115	15	1.366396345034122
1115	16	1.1897963714927293
1115	17	0.6923631337740481
1115	18	1.0865210753591157
1115	19	1.4359638243704893
1115	20	1.4671013732724347
1115	21	1.5478965177453894
1115	22	1.1892507950667586
1115	23	0.932154662868399
1115	24	0.9715273331932366
1115	25	1.1900636582633162
1116	1	1.710562263236035
1116	2	1.8719486936815848
1116	3	2.770888561289027
1116	4	2.2047486106703644
1116	5	1.4696592174369378
1116	6	0.882254997494325
1116	7	-2.7657477378470645
1116	8	0.8533265803936594
1116	9	0.9053819447327172
1116	10	1.6894233238286773
1116	11	0.185518811720371
1116	12	1.6880216889179422
1116	13	-4.421826385356642
1116	14	0.5639623862944205
1116	15	1.2421852075574726
1116	16	1.5110534168742795
1116	17	1.7459354255385122
1116	18	1.892237998991316
1116	19	2.3731905086826153
1116	20	2.3952725871888596
1116	21	2.579038796050181
1116	22	2.3696182022481906
1116	23	2.3317737530460576
1116	24	2.5427011284021077
1116	25	2.493692345306041
1201	1	1.9463969748019956
1201	2	2.049777759675095
1201	3	2.861309243483092
1201	4	2.2210409369197883
1201	5	1.8257010991286187
1201	6	1.6359197698683268
1201	7	1.7945701053950351
1201	8	1.9946633624173629
1201	9	2.335407685558664
1201	10	2.590652665883219
1201	11	2.2430014335540633
1201	12	2.4356248076614904
1201	13	2.433993155287748
1201	14	3.196926809239816
1201	15	2.5659841555411127
1201	16	2.0867013520123727
1201	17	1.8791259200856842
1201	18	2.036024760496968
1201	19	2.0267080485062827
1201	20	2.183573070917422
1201	21	2.0846841198001336
1201	22	1.2962237707225164
1201	23	0.7475875735069053
1201	24	1.2889110093974203
1201	25	1.3947635118504316
1202	1	1.760892318629292
1202	2	1.9848133791113405
1202	3	2.7279085846660003
1202	4	2.1256455896730273
1202	5	1.663542276336738
1202	6	1.4912189161416762
1202	7	1.625729190345968
1202	8	1.9419908218681954
1202	9	2.110262985160862
1202	10	-1.7200609766458186
1202	11	0.7094858806834203
1202	12	1.8499044971739913
1202	13	2.2399890747251305
1202	14	2.9156483849296926
1202	15	2.371165102277228
1202	16	2.1662196632521535
1202	17	1.737016109638077
1202	18	1.9478184983151188
1202	19	2.1031378981637623
1202	20	2.1264196622798472
1202	21	2.2674486439814565
1202	22	2.0822716660412652
1202	23	1.6645287472955594
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2211	17	-0.07821718878284756
2211	18	0.36628828228591104
2211	19	0.6522772815331415
2211	20	0.9301510127496009
2211	21	0.9721814811958005
2211	22	0.7093584326707365
2211	23	0.5843355712718541
2211	24	0.8430249180876004
2211	25	0.7837212208081397
2212	1	1.140481846623313
2212	2	1.2436921085823975
2212	3	1.9573500864713687
2212	4	1.4484799990437622
2212	5	1.0487954941046098
2212	6	0.8656537862101032
2212	7	1.1707101908856927
2212	8	1.5024785375815242
2212	9	1.5651146565995617
2212	10	2.387918569322327
2212	11	1.4504980242417673
2212	12	1.5317559544510075
2212	13	1.860185612399171
2212	14	-4.28408761874061
2212	15	-0.600275914822511
2212	16	0.09610944955881351
2212	17	0.5761470406320939
2212	18	0.8429522538788916
2212	19	1.2869404547358698
2212	20	1.4062489513221834
2212	21	1.3286036256503984
2212	22	1.379242301337566
2212	23	1.3478786542875518
2212	24	1.257279696439742
2212	25	1.2914639851431957
2213	1	1.2689003238613488
2213	2	1.4085429760534502
2213	3	2.0209504572986887
2213	4	1.426599792997603
2213	5	0.9103862875496098
2213	6	0.5428729995264305
2213	7	0.9010608736401302
2213	8	-4.950555345719398
2213	9	-1.1197009404533382
2213	10	0.65678231816349
2213	11	0.4839380052821767
2213	12	1.1229857488927806
2213	13	-7.697754762471003
2213	14	-1.824996424495403
2213	15	-0.40962187859017263
2213	16	0.1550361414584671
2213	17	0.29667149641511514
2213	18	0.5427403525524177
2213	19	0.9597536539579679
2213	20	1.2116224622622873
2213	21	1.2793337790697485
2213	22	0.9062429914109664
2213	23	0.710369331326234
2213	24	0.7751058955294035
2213	25	0.9675470170050708
2214	1	-0.530341046428429
2214	2	-0.1668277180094968
2214	3	-6.7896132830549405
2214	4	-2.1037810676547224
2214	5	-1.1228626214742796
2214	6	-0.5426785528106954
2214	7	-0.17244297301678557
2214	8	-5.979516451083313
2214	9	-1.4976543049404292
2214	10	0.3232325732200323
2214	11	0.1224782970811269
2214	12	0.5179191012513122
2214	13	-5.7852385613271124
2214	14	-0.9918949607443466
2214	15	-0.16966302057759464
2214	16	-0.19555423417025608
2214	17	-0.04247046722297654
2214	18	-0.03428196638122216
2214	19	0.4205641273286389
2214	20	0.6475646256649448
2214	21	0.6605131034374558
2214	22	0.39284449677963806
2214	23	0.3924119239848842
2214	24	0.5488558806659682
2214	25	0.6477738924732677
2215	1	0.8719522101777848
2215	2	1.2712465989070125
2215	3	-1.8909198189432317
2215	4	0.2721982994535175
2215	5	0.47366353330984623
2215	6	0.27191929833085027
2215	7	0.8862770710513921
2215	8	1.4333970223340557
2215	9	1.7825045865095959
2215	10	2.291428345028166
2215	11	1.5599199361606146
2215	12	1.3280684196385444
2215	13	1.8105784380792507
2215	14	2.157529853276177
2215	15	1.9315472498748
2215	16	1.702130582096462
2215	17	1.3580948248089229
2215	18	1.4896215435635334
2215	19	1.6203218593149664
2215	20	1.639309062324962
2215	21	1.9765875594040416
2215	22	1.689002436271462
2215	23	1.4698978451648834
2215	24	1.387475701719218
2215	25	1.521826881247648
2216	1	1.6901183062093672
2216	2	1.6010797580331797
2216	3	2.6253999022242205
2216	4	1.5217230597725688
2216	5	1.4368646776756258
2216	6	0.9273285967292502
2216	7	0.8099816757116756
2216	8	-4.760218845648815
2216	9	-0.20246613626220267
2216	10	1.4211827555651233
2216	11	0.9383483663913559
2216	12	1.9595001466965023
2216	13	2.479797642500543
2216	14	3.455729249252702
2216	15	2.698389355636717
2216	16	2.305995327840865
2216	17	2.2079887369080566
2216	18	2.261275984140564
2216	19	2.607925353061038
2216	20	2.5570663006090975
2216	21	2.5016191393888256
2216	22	2.3474756802300405
2216	23	2.2269804800111856
2216	24	2.322616856907004
2216	25	2.251342297871279
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	167753.0
3	5.0
4	1094.0
5	7618.0
6	11917.0
7	10807.0
8	9500.0
9	7781.0
10	6984.0
11	6595.0
12	5959.0
13	5765.0
14	5932.0
15	5883.0
16	6144.0
17	6519.0
18	7421.0
19	9508.0
20	13219.0
21	16419.0
22	19708.0
23	23209.0
24	26373.0
25	29722.0
26	32941.0
27	36405.0
28	39507.0
29	45833.0
30	52158.0
31	59105.0
32	73400.0
33	91822.0
34	115736.0
35	143541.0
36	180548.0
37	209450.0
38	265597.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.98783715762044	17.92369916597326	13.192563499373938	22.895900177032363
2	14.827524806043165	20.710948673491632	40.60589181334944	23.855634707115765
3	16.73537904632963	27.10102631922917	29.58043686347346	26.58315777096774
4	10.914085871319166	16.43691276048876	36.840897584230575	35.808103783961506
5	12.835393716046784	39.33929614198403	32.85898008915101	14.966330052818178
6	31.353035711535092	36.58856343180339	17.81372175957911	14.244679097082411
7	29.250504079995526	33.15123312744124	20.115787203499977	17.482475589063263
8	26.801830971210073	34.93502425643182	19.556338080401385	18.70680669195672
9	27.430637769089056	16.83144873764705	19.041101283206263	36.69681221005763
10	13.703213557680172	31.12780949976611	32.05001596412156	23.118960978432156
11	34.35658042552123	24.667816497890993	21.882832464493777	19.092770612093997
12	23.13327456803225	27.56046978008655	28.932782194684307	20.373473457196894
13	27.72452216853874	22.93368521399513	26.271531154388782	23.070261463077347
14	21.24186996508364	22.15294735160547	28.34495310253543	28.260229580775466
15	23.55177156718389	29.903421962561104	23.38661507927226	23.15819139098274
16	22.961142366168545	29.909920395165855	24.35138862026845	22.77754861839714
17	22.079103032695564	29.574386640873644	25.82255185463872	22.523958471792067
18	21.60474423301281	28.01773097186606	28.20502434585664	22.17250044926449
19	23.019351231701336	27.2407379939071	28.090606209280832	21.649304565110732
20	23.285675569669035	26.84914448158317	27.96887131946406	21.89630862928374
21	23.986880636958883	26.466358098821964	27.68096602691991	21.865795237299245
22	23.76397536080492	26.606770701105702	27.33491288594303	22.294341052146343
23	22.779394146842375	27.04204461178618	27.65850875691711	22.520052484454332
24	23.04935002105172	27.015288454187697	27.46616036046932	22.469201164291263
25	22.649967341047883	26.78165279968511	28.25139121431403	22.31698864495298
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8632.0
1	8632.0
2	6949.0
3	5266.0
4	5266.0
5	5266.0
6	7567.5
7	9869.0
8	9869.0
9	9869.0
10	10155.0
11	10441.0
12	10441.0
13	10441.0
14	11924.5
15	13408.0
16	13408.0
17	13408.0
18	19442.5
19	25477.0
20	25477.0
21	25477.0
22	35411.0
23	45345.0
24	45345.0
25	45345.0
26	61785.5
27	78226.0
28	78226.0
29	78226.0
30	95691.0
31	113156.0
32	113156.0
33	113156.0
34	127229.0
35	141302.0
36	141302.0
37	141302.0
38	155196.0
39	169090.0
40	169090.0
41	169090.0
42	183233.5
43	197377.0
44	197377.0
45	197377.0
46	208575.5
47	219774.0
48	219774.0
49	219774.0
50	215213.0
51	210652.0
52	210652.0
53	210652.0
54	195485.0
55	180318.0
56	180318.0
57	180318.0
58	158745.0
59	137172.0
60	137172.0
61	137172.0
62	113459.5
63	89747.0
64	89747.0
65	89747.0
66	70885.5
67	52024.0
68	52024.0
69	52024.0
70	39733.0
71	27442.0
72	27442.0
73	27442.0
74	19908.0
75	12374.0
76	12374.0
77	12374.0
78	9461.0
79	6548.0
80	6548.0
81	6548.0
82	4549.0
83	2550.0
84	2550.0
85	2550.0
86	1863.5
87	1177.0
88	1177.0
89	1177.0
90	805.0
91	433.0
92	433.0
93	433.0
94	247.0
95	61.0
96	61.0
97	61.0
98	39.0
99	17.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3217515663771889
2	0.3233443959137096
3	0.33500618359180784
4	0.33739542789658894
5	0.342401463582797
6	0.28596978857463373
7	0.29467346425633634
8	0.2927962008740083
9	0.2934788421039458
10	0.40258766535561624
11	0.2933650685656229
12	0.2925686537973625
13	0.28505960026805044
14	0.2911464845683261
15	0.2841494119614672
16	0.2898380888776127
17	0.28153262058004025
18	0.28517337380637336
19	0.28545780765218065
20	0.5051545101537195
21	0.28722129749618575
22	0.2879039387261232
23	0.2831823368857225
24	0.2873919578036701
25	0.27948469689022787
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1757878.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.918168546276895
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.88313872534644	51.70925351607658
2	7.8758048511141485	9.59559214715514
3	2.8180684838093355	5.150147126149443
4	1.3454408262852635	3.27847164098751
5	0.754221104555942	2.2972884184249005
6	0.47400170766445343	1.732518955123575
7	0.32948700123945207	1.4050221272718602
8	0.22494535040954577	1.0962607015960022
9	0.17256340735971923	0.9461022061013294
>10	0.986081707701859	11.279380186771165
>50	0.08805483514799335	3.738741138376589
>100	0.04503061466633264	4.83300499988259
>500	0.0021075897996878476	0.8870033505795865
>1k	8.621958271450286E-4	1.2910833031694315
>5k	1.9159907269889525E-4	0.7601301823343057
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	7945	0.4519653809877591	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5080	0.28898478734019084	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	4652	0.2646372501390882	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3622	0.2060438779027896	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	3350	0.19057067669087388	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2597	0.14773493951229835	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1947	0.11075853955735268	No Hit
GTGGTATCAACGCAGAGTACATGGG	1764	0.10034826080080643	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	5.688676916145489E-5	0.0
12	0.0	0.0	0.0	5.688676916145489E-5	0.0
13	0.0	0.0	0.0	5.688676916145489E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCGCA	80	2.1827873E-11	17.832502	18
AAGACGT	35	0.0021648593	16.291887	5
CGTTCGC	90	2.2519089E-9	14.794372	15
TTCGCGC	95	4.825779E-9	14.01572	17
ACGGCCC	170	0.0	13.426825	15
CGGCCCC	200	0.0	12.839402	16
CGCGCAG	105	1.9630534E-8	12.68089	19
GTTCGCG	105	1.9630534E-8	12.68089	16
TAACGCA	90	5.3796066E-7	12.67183	4
CGCACCT	75	1.4730851E-5	12.671468	5
GGTATCA	2250	0.0	12.2323065	1
TCGTTCG	110	3.7540303E-8	12.104832	14
CCGATCA	110	3.7554855E-8	12.1044855	19
TCCCGAT	105	2.6810994E-7	11.775112	17
CCCGATC	105	2.6810994E-7	11.775112	18
TGCACGG	180	0.0	11.593975	12
CTTCGCG	115	6.965456E-8	11.578204	15
ATTTGCG	190	0.0	11.512914	16
TTGACCG	240	0.0	11.492057	18
TTGCGCG	175	1.8189894E-12	11.412801	18
>>END_MODULE