FastQCFastQC Report
Thu 26 May 2016
SRR1364278_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364278_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1757878
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT191251.0879594602128249No Hit
TATCAACGCAGAGTACTTTTTTTTT148360.8439721072793447No Hit
GGTATCAACGCAGAGTACTTTTTTT145620.8283851325291062No Hit
GTACTTTTTTTTTTTTTTTTTTTTT66400.3777281472320605No Hit
ACGCAGAGTACTTTTTTTTTTTTTT65950.375168242619795No Hit
GAGTACTTTTTTTTTTTTTTTTTTT49370.2808499793501028No Hit
GTGGTATCAACGCAGAGTACATGGG30340.17259445763585413No Hit
GTGGTATCAACGCAGAGTACTTTTT27610.15706436965477696No Hit
ATCAACGCAGAGTACTTTTTTTTTT23790.13533362383510117No Hit
GCAGAGTACTTTTTTTTTTTTTTTT19410.11041721894238395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG2250.014.3563292
TAACGCA2450.014.35016254
ATAACGC2350.014.1509073
ACCGAGT1301.8189894E-1213.8849698
CGCCAGT1001.01154E-813.29617612
AAGACCG751.4728428E-512.6716775
CGATACA604.0808265E-412.6709571
GGACCGA1352.7284841E-1112.6669896
GGTATCA38900.012.4592291
CCGAGTG1155.322363E-912.391629
GTGGTAT12050.012.3817841
TGGTATC12300.011.894952
CACGTCA802.8677825E-511.87462710
CTTCGCG1459.822543E-1111.79038415
CGAGTGG1052.7215538E-711.76153510
TCCCGGA658.0207095E-411.6929382
CGGTTTG1406.8394E-1011.53272614
ACGGCCC2650.011.46905315
AACGCCG1251.8311766E-811.40029056
TCGCGGT1354.7457434E-911.25666317
Produced by FastQC (version 0.11.5)