##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364278_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1757878
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.435563787703128	31.0	30.0	34.0	26.0	34.0
2	29.48255624110433	31.0	30.0	34.0	25.0	34.0
3	29.498594896801713	31.0	30.0	34.0	25.0	34.0
4	32.83819980681253	35.0	33.0	37.0	26.0	37.0
5	32.41764388654958	35.0	32.0	37.0	25.0	37.0
6	32.32842324666444	35.0	32.0	37.0	23.0	37.0
7	32.056054515728626	35.0	32.0	37.0	19.0	37.0
8	31.943085356321657	35.0	32.0	37.0	18.0	37.0
9	32.69200592987682	37.0	32.0	39.0	15.0	39.0
10	32.78398557806628	37.0	32.0	39.0	17.0	39.0
11	32.71953400634174	37.0	32.0	39.0	16.0	39.0
12	32.67997949800839	37.0	32.0	39.0	16.0	39.0
13	32.7022034521167	37.0	32.0	39.0	16.0	39.0
14	33.598642795461345	38.0	32.0	41.0	16.0	41.0
15	33.62478966117103	38.0	32.0	41.0	16.0	41.0
16	33.56185241524156	38.0	32.0	41.0	13.0	41.0
17	33.551749325038486	38.0	32.0	41.0	11.0	41.0
18	33.5032357194299	38.0	32.0	41.0	11.0	41.0
19	33.432744479423484	39.0	32.0	41.0	10.0	41.0
20	33.3509151374555	39.0	32.0	41.0	10.0	41.0
21	33.288345380054814	38.0	32.0	41.0	8.0	41.0
22	33.22544340392223	38.0	32.0	41.0	2.0	41.0
23	32.95247736191021	38.0	32.0	41.0	2.0	41.0
24	32.87622064784928	38.0	32.0	41.0	2.0	41.0
25	32.70071984517697	38.0	32.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.4744523359802244
1101	2	0.7193607518858407
1101	3	-1.5537632244048218
1101	4	-0.30593917523952285
1101	5	0.6975423226464343
1101	6	1.0788395232699344
1101	7	1.2472140975505042
1101	8	1.392943862672773
1101	9	2.09913135650536
1101	10	1.7493070983737908
1101	11	1.577246265598653
1101	12	1.8455198957609085
1101	13	1.583552069390521
1101	14	1.843742007317367
1101	15	1.7746114734836382
1101	16	1.846754228906569
1101	17	1.8308936244364773
1101	18	1.80131050196254
1101	19	1.7779248566207713
1101	20	2.1321537985013705
1101	21	1.8496860905102182
1101	22	2.0423290595545325
1101	23	2.2994634327860197
1101	24	2.363096162771548
1101	25	2.414058423581892
1102	1	0.8106326664241976
1102	2	0.809515025713555
1102	3	0.8483428680071547
1102	4	0.7610395139327508
1102	5	1.0222001927060802
1102	6	1.03530893268821
1102	7	1.2365434514663747
1102	8	1.3418250085165155
1102	9	2.0325924976842416
1102	10	1.5243450599219557
1102	11	1.6055570124058391
1102	12	1.7142645704340111
1102	13	1.3264186303836638
1102	14	1.4683894052257926
1102	15	1.560219881669049
1102	16	1.6358608116056246
1102	17	1.5582088028676537
1102	18	1.6510772585945048
1102	19	1.5929250232231738
1102	20	1.7455767163283085
1102	21	1.886978373010777
1102	22	1.8171644801780076
1102	23	2.1517211333872126
1102	24	2.1985822371904646
1102	25	2.053816659690824
1103	1	-1.532000607622816
1103	2	-1.5328442493909975
1103	3	-1.639149320619488
1103	4	-1.5643270197402757
1103	5	-4.525634254248686
1103	6	-8.958341021645303
1103	7	-9.409945035771042
1103	8	-10.302707030131756
1103	9	-10.460177775834001
1103	10	-9.252249661993151
1103	11	-12.99079447536035
1103	12	-10.898243240939344
1103	13	-12.354310110663302
1103	14	-11.79474091251684
1103	15	-11.77942824600484
1103	16	-6.328202063941173
1103	17	-4.293509699271464
1103	18	-3.3536239864182313
1103	19	-3.761946870253727
1103	20	-9.645136161621988
1103	21	-5.9531504545621665
1103	22	-11.49515105123573
1103	23	-13.639616654431851
1103	24	-13.75022322356039
1103	25	-16.55484074549112
1104	1	0.4386711114177331
1104	2	0.6656105298330672
1104	3	0.7363219974786617
1104	4	0.757082648779658
1104	5	0.9147044858741253
1104	6	0.962472853959504
1104	7	0.9860394511304165
1104	8	1.113015439036431
1104	9	1.361683889669493
1104	10	1.2964535984742085
1104	11	1.2274287315231192
1104	12	1.2367609979960932
1104	13	1.2511784910500694
1104	14	1.2506320903825028
1104	15	1.2652609065359428
1104	16	0.8339477554942292
1104	17	0.7502895110218901
1104	18	0.9004474993637714
1104	19	1.2332287059838762
1104	20	1.402344599231796
1104	21	1.3481629128387809
1104	22	1.032348164209921
1104	23	1.645848715104954
1104	24	1.688918778434953
1104	25	1.8147382181477099
1105	1	0.5735846614000053
1105	2	0.6896880498489644
1105	3	0.21042503800642365
1105	4	0.6187424515325688
1105	5	0.689074397385447
1105	6	0.8940919774519713
1105	7	0.892769944669844
1105	8	0.8752227123479344
1105	9	0.8881055838693612
1105	10	0.5892244064006746
1105	11	1.0843843716708363
1105	12	1.3359177553623809
1105	13	0.9857127972875048
1105	14	0.9681132628454634
1105	15	0.7695666191197432
1105	16	1.1707398993478648
1105	17	0.9810411204643188
1105	18	0.9949020422555321
1105	19	1.1513620667591127
1105	20	1.4370558186538744
1105	21	1.4186766486498001
1105	22	1.0991258437662594
1105	23	1.7143936929730472
1105	24	1.5998801534210045
1105	25	1.8457668976107726
1106	1	0.01868435686496639
1106	2	0.27541625379908297
1106	3	0.1351903928751561
1106	4	-1.053890038880759
1106	5	-0.08271623330249867
1106	6	0.2210835600328025
1106	7	0.4484707581377094
1106	8	0.5773146383406171
1106	9	1.1341980517812615
1106	10	0.5905648318072565
1106	11	0.7357253272157891
1106	12	0.8022888404378037
1106	13	0.6509529205152234
1106	14	0.02990336339504296
1106	15	0.5841590899768576
1106	16	0.5943235168942635
1106	17	0.4495311096848411
1106	18	0.56506868241528
1106	19	-0.6454764236424353
1106	20	0.5256788137000257
1106	21	0.3199324875348637
1106	22	0.7647622748747764
1106	23	0.6582092755067563
1106	24	0.7990075456703778
1106	25	1.1230742659450144
1107	1	0.2561404894759498
1107	2	0.04545901825341048
1107	3	-0.06909028782672166
1107	4	0.05566755195694384
1107	5	0.27215662038660327
1107	6	0.1797947440846599
1107	7	-0.020696952640459898
1107	8	-0.12029666982816067
1107	9	0.8119255945604209
1107	10	0.6800661876816321
1107	11	0.5428464617973532
1107	12	0.5234389513195055
1107	13	0.41061045194150836
1107	14	0.5201636559815555
1107	15	0.4326584517453185
1107	16	0.40964483853355205
1107	17	0.2339710835673472
1107	18	0.3376703729935713
1107	19	0.6471726784216685
1107	20	0.5186095611662225
1107	21	0.4466052718126008
1107	22	0.3650216851135397
1107	23	0.7917395488580894
1107	24	0.9472991439550924
1107	25	0.6311658654061958
1108	1	0.038051542585588294
1108	2	0.054561672818277174
1108	3	0.09590687913161489
1108	4	-0.0799967745947896
1108	5	0.2103009925758812
1108	6	0.2481757790184247
1108	7	-0.1367935038586694
1108	8	0.3246412107864032
1108	9	0.6187290451762095
1108	10	0.378555505618408
1108	11	0.505269566675878
1108	12	0.4340217374301716
1108	13	2.964910789913233E-4
1108	14	0.009932852372635637
1108	15	-0.08538107377087556
1108	16	-0.6550742366689875
1108	17	-0.733783882942916
1108	18	-0.7780851997007332
1108	19	-0.5433505800299159
1108	20	0.061375700575034386
1108	21	-0.20176768388480326
1108	22	0.08942512955165682
1108	23	-0.44096683122895897
1108	24	0.047462610941714445
1108	25	-0.106062522153902
1109	1	0.1854092597800161
1109	2	0.15643541758779733
1109	3	0.13961281471404519
1109	4	0.15083893291676276
1109	5	0.26782370327946836
1109	6	0.562043427661628
1109	7	0.654866499648854
1109	8	0.5385038590406879
1109	9	1.3520756967756853
1109	10	0.5802669672345075
1109	11	0.6291906784145169
1109	12	0.790786563100184
1109	13	0.8956983798249283
1109	14	0.4716663495190332
1109	15	0.28282401367795273
1109	16	0.5160945112384638
1109	17	0.534521526318386
1109	18	0.39751010022356326
1109	19	0.6979367857990226
1109	20	1.0583953963098267
1109	21	1.0107504153490012
1109	22	0.5452758019952668
1109	23	1.0195751481230602
1109	24	0.70051738959841
1109	25	0.6863085269953331
1110	1	-0.06957869321626475
1110	2	-0.09373646217181175
1110	3	-0.11537928996725455
1110	4	0.08236234509889329
1110	5	0.027992603315432518
1110	6	0.2037084099303783
1110	7	0.2322095446037693
1110	8	0.07977172670679522
1110	9	-3.368158026483904
1110	10	-1.4073344406374702
1110	11	-0.41522120100346527
1110	12	-0.1289691944565945
1110	13	-0.3089234657248028
1110	14	-0.029603947475905557
1110	15	0.14042489487902543
1110	16	-0.3529760522234824
1110	17	-0.13620845378199675
1110	18	-0.04829961595301313
1110	19	-0.09023131521550454
1110	20	0.2054778530445205
1110	21	0.005997684963695349
1110	22	0.3258292382144674
1110	23	0.45023512844169744
1110	24	0.4894461979272151
1110	25	0.46187733156626365
1111	1	0.29697674323266554
1111	2	0.3190818118579486
1111	3	0.29700386067188944
1111	4	0.16198272155266835
1111	5	0.4998435778765611
1111	6	0.2671240420983878
1111	7	0.4149267893217754
1111	8	0.6405031374286949
1111	9	1.3041522770074536
1111	10	0.7705762755007086
1111	11	1.0981667676906994
1111	12	0.9706942332783441
1111	13	0.8551699215523101
1111	14	0.6244254103857116
1111	15	0.6954622405687161
1111	16	0.6399899332301402
1111	17	0.694213010768415
1111	18	0.5181863736501313
1111	19	0.8236012861884134
1111	20	1.0878720690731996
1111	21	0.8768827432298849
1111	22	0.9237795476995814
1111	23	1.2349585513355308
1111	24	1.1535424668161767
1111	25	1.3966246718385165
1112	1	-0.5034233102048908
1112	2	-0.5450800697869056
1112	3	-0.444603580206536
1112	4	-0.42357324248546036
1112	5	-0.3284460838144838
1112	6	-0.43319736170220935
1112	7	-0.3808301230320943
1112	8	-0.7056725365298853
1112	9	-0.04929258713609386
1112	10	-0.3184346726693832
1112	11	-0.15421431742316827
1112	12	-1.0729834357995394
1112	13	-0.77734743435337
1112	14	-1.5752163470469576
1112	15	-0.8905979650754787
1112	16	-1.0426395712319447
1112	17	-1.1670331590796437
1112	18	-1.2796384817445272
1112	19	-0.8192181174705553
1112	20	-0.8112694395031781
1112	21	-0.9229755267091271
1112	22	-0.6641676716640958
1112	23	-0.275782950008697
1112	24	-0.4183903103446909
1112	25	-0.42414443221354503
1113	1	-2.010791550273712
1113	2	-1.9336883792788377
1113	3	-1.9350885620782563
1113	4	-1.7552555626169024
1113	5	-1.462124649038124
1113	6	-1.5470216866033297
1113	7	-1.9562898828165842
1113	8	-2.016937320051575
1113	9	-11.308493043063422
1113	10	-4.312385098989083
1113	11	-2.865444714021031
1113	12	-13.014657922685917
1113	13	-4.793586839483638
1113	14	-4.117109178876262
1113	15	-3.5931318364960383
1113	16	-3.288194069396276
1113	17	-3.1885460274240174
1113	18	-3.6112011690465557
1113	19	-4.235717708975539
1113	20	-16.067906506563087
1113	21	-18.243599324744864
1113	22	-13.300166452491347
1113	23	-19.70444300821398
1113	24	-20.497301687667235
1113	25	-16.714637589997224
1114	1	-0.244653240702565
1114	2	-0.20821316570885884
1114	3	-0.14777009806797992
1114	4	-0.5056059296422077
1114	5	-0.22374409914597848
1114	6	-0.12600063078241064
1114	7	-0.25756146464071605
1114	8	0.0817653864356167
1114	9	0.7203137052288042
1114	10	9.520984089306239E-5
1114	11	-0.04030411454033356
1114	12	-0.07617343962967738
1114	13	-0.08608325131487504
1114	14	-0.0968856682529875
1114	15	0.0037945906535767904
1114	16	-0.12816234624235534
1114	17	-0.15651928243930513
1114	18	-0.2172751631823573
1114	19	-0.5221996377626112
1114	20	-0.8268038976113168
1114	21	-1.143311667683264
1114	22	-1.1324622648660316
1114	23	-0.6804291359733057
1114	24	-0.39302871886046375
1114	25	-0.656604618679637
1115	1	-0.46212454303617534
1115	2	-0.29365508806874274
1115	3	-0.40321320612873635
1115	4	-0.11732435956251663
1115	5	-0.6277520856882752
1115	6	-0.48391687795446003
1115	7	-0.8412237770326669
1115	8	-0.5698280579089605
1115	9	-0.2088528423384446
1115	10	-0.5965055339746748
1115	11	-0.4091589815777432
1115	12	-0.4182203510377107
1115	13	-0.5849258696388233
1115	14	-0.8453873975765376
1115	15	-0.7255493356466474
1115	16	-0.5375157793227743
1115	17	-0.6039204802089202
1115	18	-0.6775367160905574
1115	19	-0.7158010347723192
1115	20	-1.0773274057190747
1115	21	-0.4269765737958693
1115	22	-1.1367694291459465
1115	23	-0.7578244369984084
1115	24	-0.26047212546375675
1115	25	-0.1940206271235212
1116	1	-2.4815578188284206
1116	2	-2.364713675780589
1116	3	-2.276754851087695
1116	4	-2.309720879597883
1116	5	-4.157670049356824
1116	6	-3.7699206977946425
1116	7	-5.426184069385787
1116	8	-4.3320075581716
1116	9	-3.0885348589046906
1116	10	-4.5646250146225
1116	11	-3.8300678263983485
1116	12	-5.226154951850447
1116	13	-4.602736841259645
1116	14	-4.194532176609094
1116	15	-5.9566160979883485
1116	16	-6.363134328105765
1116	17	-7.1266759749888635
1116	18	-6.027009124834937
1116	19	-5.870034870005721
1116	20	-5.902191331969089
1116	21	-5.147011345513153
1116	22	-4.808249746119635
1116	23	-5.043768638972995
1116	24	-5.9589539645728635
1116	25	-5.489615941051866
1201	1	1.0034446060319944
1201	2	1.133906765170952
1201	3	0.6455916676832096
1201	4	1.164997401362612
1201	5	1.3481399161977237
1201	6	1.457490919152022
1201	7	1.623136845346174
1201	8	1.5929339342988982
1201	9	2.2696145373672394
1201	10	2.116625964648051
1201	11	1.8445504757019577
1201	12	2.0580327199105
1201	13	1.9104690729127327
1201	14	2.0034018423172526
1201	15	2.0818847165160435
1201	16	1.8470426245286262
1201	17	1.8383881283723653
1201	18	1.8596536454748218
1201	19	2.01370364299526
1201	20	2.1739853470913104
1201	21	2.2806857832034098
1201	22	2.2541494978213237
1201	23	2.5741240951503883
1201	24	2.5389293188124924
1201	25	2.625574628106399
1202	1	0.9105484324143127
1202	2	0.9763954524380019
1202	3	0.9868582378449524
1202	4	1.0140612655870171
1202	5	1.073359844844866
1202	6	1.2026125951462348
1202	7	1.261422781083013
1202	8	1.489671991814312
1202	9	1.8168806743966357
1202	10	1.516175279045143
1202	11	1.65642035362702
1202	12	1.6986812786056333
1202	13	1.5507923264205346
1202	14	1.679832147903518
1202	15	1.6999659402288998
1202	16	1.558648291723415
1202	17	1.5177796914102188
1202	18	1.6665650699103267
1202	19	1.7242811907822926
1202	20	2.0873292427748
1202	21	2.0154790764909976
1202	22	1.970168099715707
1202	23	2.297045686797773
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2211	11	0.14060933209979254
2211	12	0.3199001180490839
2211	13	0.27092850049080397
2211	14	0.12071921421089371
2211	15	0.04549331131108403
2211	16	0.13511161768236946
2211	17	-0.027578447424716046
2211	18	0.02183857918517873
2211	19	-0.2161546343206453
2211	20	0.33769790571911784
2211	21	0.5344957020980772
2211	22	0.3433836534961543
2211	23	0.5812329185303966
2211	24	0.6408856675485026
2211	25	0.8055828684536195
2212	1	-0.07073538273732893
2212	2	-0.12160806963497706
2212	3	-0.08227324333087438
2212	4	-0.031406032015411256
2212	5	-0.3010820730070769
2212	6	-0.11418004496711376
2212	7	-0.16689444010781074
2212	8	-0.18316489564804428
2212	9	0.33796809737686573
2212	10	-0.08055009338474406
2212	11	0.15033152336356892
2212	12	-0.044368811128563834
2212	13	-0.30687131970252324
2212	14	-0.1736655761171022
2212	15	0.12326104729383047
2212	16	0.029295864807792782
2212	17	-0.11321086774273681
2212	18	-0.02058586587617839
2212	19	0.02511423879406749
2212	20	0.3200620594828649
2212	21	0.497529963306782
2212	22	0.14365133713098288
2212	23	0.4157928818936085
2212	24	0.4674914485153394
2212	25	0.48916500639681004
2213	1	-0.30647909365930914
2213	2	-0.1918442977913628
2213	3	-0.3543663962692598
2213	4	-0.16496590459580318
2213	5	-0.2992850939950671
2213	6	0.002512760843444539
2213	7	-0.1787316385492197
2213	8	-0.1333541430498144
2213	9	0.5490134540193452
2213	10	0.04110496750202941
2213	11	0.23887979016407002
2213	12	0.05855459072417801
2213	13	-0.19212047802209042
2213	14	-0.16298059113839258
2213	15	-0.014663713794398348
2213	16	-0.2417977272682137
2213	17	-0.18080460271272614
2213	18	-0.26036615765872995
2213	19	-0.14188491778288892
2213	20	0.16351298411152015
2213	21	-0.17124735209858954
2213	22	0.09376042449148514
2213	23	-0.10123032269572718
2213	24	-0.0928349074091841
2213	25	0.3137183755581958
2214	1	-1.2916477639169806
2214	2	-1.0233262416908175
2214	3	-1.0294314981135173
2214	4	-0.9190129206126372
2214	5	-1.2099950905467516
2214	6	-1.0078999670130102
2214	7	-0.9931800862120959
2214	8	-1.0351793728385168
2214	9	-11.197660109281049
2214	10	-4.4612803165084784
2214	11	-2.3366787777142655
2214	12	-1.5941202329954898
2214	13	-1.3095321000390037
2214	14	-1.3145079576944774
2214	15	-1.4881616150774093
2214	16	-1.6253754154690263
2214	17	-1.3705309966056411
2214	18	-1.932867614275338
2214	19	-1.9771766572803848
2214	20	-1.602731561007925
2214	21	-2.1493101114655246
2214	22	-2.008860296325647
2214	23	-2.4580288577556004
2214	24	-2.879288967505026
2214	25	-10.49440895056934
2215	1	-0.5588798117502058
2215	2	-0.2731516268862606
2215	3	-0.24888112722418398
2215	4	-0.2669958923266478
2215	5	-0.3407941471454805
2215	6	-0.28473368986481873
2215	7	-0.5751658817122198
2215	8	-0.7146625344093529
2215	9	0.34318879142115577
2215	10	0.3064122009931154
2215	11	0.4186848114890296
2215	12	0.34363694279054613
2215	13	0.20438440573959582
2215	14	0.0037439689512623886
2215	15	-0.46639626742548046
2215	16	-0.14581096308565122
2215	17	-0.17028658936006025
2215	18	-0.0376151046889035
2215	19	-0.10513427909626927
2215	20	0.14295949111981088
2215	21	-0.10222962821303838
2215	22	-0.16378962145146403
2215	23	0.17818272297633797
2215	24	0.4704104704117924
2215	25	0.5559686238625616
2216	1	-2.4798637521552465
2216	2	-2.404015482800034
2216	3	-2.4420698480760734
2216	4	-2.434172703283597
2216	5	-4.055767845495737
2216	6	-4.734448309147137
2216	7	-3.468092202030398
2216	8	-5.059216041280191
2216	9	-3.260611278135361
2216	10	-3.777188997577472
2216	11	-2.9885233378054075
2216	12	-4.243084822618261
2216	13	-4.032456240662178
2216	14	-4.332542831325831
2216	15	-3.838944139649975
2216	16	-4.913503572563727
2216	17	-5.440693229638214
2216	18	-4.6342735490299525
2216	19	-6.088887052117439
2216	20	-5.064303526325229
2216	21	-5.433116085256202
2216	22	-5.311236404557377
2216	23	-4.980261381161327
2216	24	-4.9081018256450015
2216	25	-4.976659209925799
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	69084.0
3	3.0
4	1051.0
5	9664.0
6	12830.0
7	10251.0
8	8018.0
9	6583.0
10	5606.0
11	5207.0
12	4648.0
13	4207.0
14	4145.0
15	4328.0
16	4492.0
17	4648.0
18	5512.0
19	6639.0
20	9018.0
21	11433.0
22	13878.0
23	16473.0
24	19049.0
25	21520.0
26	23956.0
27	26221.0
28	29412.0
29	34216.0
30	41726.0
31	51400.0
32	63669.0
33	78568.0
34	97636.0
35	122842.0
36	161488.0
37	236256.0
38	532201.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.114909041248666	18.983720175942732	13.295796607469033	23.605574175339566
2	17.179079778298487	21.05959579238153	37.61962705768779	24.141697371632194
3	19.609972489527134	25.736001856786256	29.111780353107957	25.542245300578653
4	12.80421480438092	17.044391752436564	35.25660426304639	34.89478918013613
5	13.107694163666359	38.70424208695177	33.361216717055925	14.826847032325945
6	31.764599459976722	36.923437533779904	16.908952712120712	14.403010294122668
7	28.776479437487446	32.698431844910765	20.35221897842613	18.172869739175656
8	27.456757584137637	35.02376782302218	18.62607529910357	18.893399293736607
9	27.007373205281482	15.68181805248821	18.96206074173637	38.348748000493934
10	15.975309049459865	30.61579986376386	30.48201074068868	22.926880346087593
11	35.04991896419857	23.307922387733544	21.67593874057617	19.966219907491713
12	23.89478151639139	27.090852406937955	28.566316522467332	20.448049554203322
13	29.298794536445506	21.585477549023512	25.880716565310642	23.235011349220343
14	22.408213978509306	21.251188997055383	27.792322606190524	28.54827441824479
15	24.340530306478186	28.91449194891727	23.744606821346974	23.00037092325757
16	23.069496586812278	28.711624750176508	25.189717939104796	23.029160723906422
17	22.241382154940577	28.257531493941862	26.968531375037763	22.532554976079794
18	21.607184207397108	26.16945630856954	29.758317009296025	22.46504247473733
19	23.113406414091042	25.736302763030704	29.55492123061062	21.595369592267634
20	23.03946446127517	25.225644503513816	29.812270145286384	21.922620889924627
21	23.72975470105115	24.83762798032875	29.471261176588392	21.96135614203171
22	23.458005230678378	24.904833399423225	29.502243547986097	22.134917821912296
23	22.465660372206063	25.10314404438437	30.138655634293205	22.292539949116364
24	22.89066082292531	25.43108401629785	29.54292294021202	22.135332220564827
25	22.410206620864805	25.094256355648692	30.250239876090117	22.245297147396386
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1138.0
1	1138.0
2	1589.0
3	2040.0
4	2040.0
5	2040.0
6	5266.5
7	8493.0
8	8493.0
9	8493.0
10	8848.5
11	9204.0
12	9204.0
13	9204.0
14	9159.0
15	9114.0
16	9114.0
17	9114.0
18	15379.0
19	21644.0
20	21644.0
21	21644.0
22	33209.5
23	44775.0
24	44775.0
25	44775.0
26	64166.5
27	83558.0
28	83558.0
29	83558.0
30	98817.0
31	114076.0
32	114076.0
33	114076.0
34	128780.0
35	143484.0
36	143484.0
37	143484.0
38	153403.0
39	163322.0
40	163322.0
41	163322.0
42	175869.0
43	188416.0
44	188416.0
45	188416.0
46	202936.0
47	217456.0
48	217456.0
49	217456.0
50	215693.5
51	213931.0
52	213931.0
53	213931.0
54	200386.5
55	186842.0
56	186842.0
57	186842.0
58	164950.5
59	143059.0
60	143059.0
61	143059.0
62	119799.0
63	96539.0
64	96539.0
65	96539.0
66	75976.0
67	55413.0
68	55413.0
69	55413.0
70	42607.0
71	29801.0
72	29801.0
73	29801.0
74	21511.0
75	13221.0
76	13221.0
77	13221.0
78	10412.5
79	7604.0
80	7604.0
81	7604.0
82	5308.5
83	3013.0
84	3013.0
85	3013.0
86	2092.5
87	1172.0
88	1172.0
89	1172.0
90	847.0
91	522.0
92	522.0
93	522.0
94	281.0
95	40.0
96	40.0
97	40.0
98	20.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.027760743350789985
2	0.001080848614067643
3	1.1377353832290979E-4
4	0.004209620917947662
5	0.031799703961253284
6	0.01001207137241606
7	0.01905706766908739
8	0.02616791381426925
9	0.032368571652867835
10	0.036123098417523855
11	0.03583866457171658
12	0.007167732914343316
13	0.002730564919749835
14	0.00534735630117676
15	0.0058024504544683985
16	0.00779348737511932
17	0.009044996296671328
18	0.013937258444556448
19	0.015871408596045914
20	0.013994145213717902
21	0.01058093906403061
22	0.009727637526608787
23	0.008077921220926595
24	0.005688676916145489
25	0.10029137403164497
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1757878.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.810129482995684
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.27454385754301	31.36892710348862
2	12.910025189850662	11.053597000124856
3	5.046966324090559	6.4818384549190675
4	2.5850005565494842	4.426568341579973
5	1.516364992888663	3.245789084452275
6	0.9214353364442982	2.366805963803286
7	0.6334002670553076	1.8981163213041237
8	0.46323591345958975	1.5864951549103057
9	0.3591056336231553	1.383602280613244
>10	1.9924789361382453	16.072623347567436
>50	0.1801188593882542	5.363915384330452
>100	0.1099580712692306	8.319718331756611
>500	0.004821422203319639	1.3810835316575367
>1k	0.0018749975235131929	1.5100297647486112
>5k	2.678567890733132E-4	0.7588276544954957
>10k+	4.017851836099699E-4	2.782062280248201
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	19125	1.0879594602128249	No Hit
TATCAACGCAGAGTACTTTTTTTTT	14836	0.8439721072793447	No Hit
GGTATCAACGCAGAGTACTTTTTTT	14562	0.8283851325291062	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	6640	0.3777281472320605	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	6595	0.375168242619795	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	4937	0.2808499793501028	No Hit
GTGGTATCAACGCAGAGTACATGGG	3034	0.17259445763585413	No Hit
GTGGTATCAACGCAGAGTACTTTTT	2761	0.15706436965477696	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	2379	0.13533362383510117	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	1941	0.11041721894238395	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	5.688676916145489E-5	0.0	0.0	0.0	0.0
8	5.688676916145489E-5	0.0	0.0	0.0	0.0
9	5.688676916145489E-5	0.0	0.0	0.0	0.0
10	5.688676916145489E-5	0.0	0.0	0.0	0.0
11	5.688676916145489E-5	0.0	0.0	0.0	0.0
12	1.1377353832290978E-4	0.0	0.0	5.688676916145489E-5	0.0
13	1.1377353832290978E-4	0.0	0.0	5.688676916145489E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAACG	225	0.0	14.356329	2
TAACGCA	245	0.0	14.3501625	4
ATAACGC	235	0.0	14.150907	3
ACCGAGT	130	1.8189894E-12	13.884969	8
CGCCAGT	100	1.01154E-8	13.296176	12
AAGACCG	75	1.4728428E-5	12.671677	5
CGATACA	60	4.0808265E-4	12.670957	1
GGACCGA	135	2.7284841E-11	12.666989	6
GGTATCA	3890	0.0	12.459229	1
CCGAGTG	115	5.322363E-9	12.39162	9
GTGGTAT	1205	0.0	12.381784	1
TGGTATC	1230	0.0	11.89495	2
CACGTCA	80	2.8677825E-5	11.874627	10
CTTCGCG	145	9.822543E-11	11.790384	15
CGAGTGG	105	2.7215538E-7	11.761535	10
TCCCGGA	65	8.0207095E-4	11.692938	2
CGGTTTG	140	6.8394E-10	11.532726	14
ACGGCCC	265	0.0	11.469053	15
AACGCCG	125	1.8311766E-8	11.4002905	6
TCGCGGT	135	4.7457434E-9	11.256663	17
>>END_MODULE