FastQCFastQC Report
Thu 26 May 2016
SRR1364277_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364277_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3078675
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT70810.23000154287152755No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN69500.2257464656061455No Hit
TATCAACGCAGAGTACTTTTTTTTT50920.16539582775057451No Hit
GGTATCAACGCAGAGTACTTTTTTT39260.12752239193809026No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA31860.10348607761455822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGG851.7149432E-814.53179414
GGTATCA36850.013.8482631
ACATGGT21100.012.4774723
CTCAACG3200.012.17744354
ATAACGC1251.4024408E-912.1657633
TAACGCA1501.4551915E-1112.0388394
TCGACCG802.866077E-511.87597818
GTATCAA97850.011.5569881
CATGGTA18650.011.1606014
TACATGT23100.010.9435062
TATAACG1502.1227606E-910.7707152
CTCGACG1201.5314217E-610.288321513
CGCGCCA1303.8597682E-710.22794310
CAACGCG2350.010.1039216
GTACTAG1606.148184E-910.0968861
ACATGTG21000.09.9570973
CGTCACT2105.456968E-129.9554731
TCAACTC22000.09.6771534
CAACTCA22000.09.6703715
TTTACGC900.00110934789.50453
Produced by FastQC (version 0.11.5)