##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364277_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3078675 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.668803300120995 30.0 23.0 31.0 7.0 33.0 2 25.309504251017078 30.0 25.0 31.0 7.0 34.0 3 24.93042006707431 30.0 23.0 31.0 7.0 34.0 4 28.009725287664335 33.0 26.0 35.0 7.0 37.0 5 28.39456194629183 33.0 27.0 35.0 2.0 37.0 6 29.03321818639512 35.0 28.0 35.0 2.0 37.0 7 28.989296369379684 35.0 29.0 36.0 2.0 37.0 8 27.189841409047723 33.0 22.0 35.0 2.0 37.0 9 27.076720991985187 32.0 17.0 37.0 2.0 39.0 10 28.758876464712905 34.0 27.0 37.0 2.0 39.0 11 29.24148408000195 35.0 27.0 38.0 2.0 39.0 12 27.884609125679066 34.0 20.0 38.0 2.0 39.0 13 27.61715640657101 33.0 18.0 37.0 2.0 39.0 14 28.10411914216343 33.0 18.0 39.0 2.0 40.0 15 27.613025408657947 33.0 17.0 38.0 2.0 40.0 16 28.42390866200557 34.0 23.0 39.0 2.0 40.0 17 28.99038547427059 34.0 25.0 39.0 2.0 41.0 18 29.552010199192836 36.0 27.0 39.0 2.0 41.0 19 30.20679220768675 36.0 28.0 40.0 2.0 41.0 20 30.226662768885966 36.0 28.0 40.0 2.0 41.0 21 30.390848335728844 36.0 29.0 40.0 2.0 41.0 22 30.277848425052987 36.0 29.0 40.0 2.0 41.0 23 29.93520784103551 36.0 28.0 40.0 2.0 41.0 24 29.883724004644854 36.0 28.0 40.0 2.0 41.0 25 29.599113904520614 36.0 27.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.747534216310445 1101 2 -0.8868574166969729 1101 3 -3.2730185626157784 1101 4 -3.2981671830901718 1101 5 -4.1922318479103815 1101 6 -2.1220821457621177 1101 7 -1.6605139089866263 1101 8 -5.464468879137655 1101 9 -6.4292461473699944 1101 10 -6.853645376308005 1101 11 -3.7251453130158403 1101 12 -0.9936461436352566 1101 13 -0.40321348289536374 1101 14 -7.843388958609669 1101 15 -9.650785228656684 1101 16 -4.752050983594199 1101 17 -2.653806299989977 1101 18 -7.237054982469605 1101 19 -3.8073124407865393 1101 20 -2.64209044171378 1101 21 -2.5417555167166554 1101 22 -2.575959985601653 1101 23 -7.57380335329611 1101 24 -4.296773444132548 1101 25 -3.3300296246892707 1102 1 1.5796875713809406 1102 2 1.1697466325513695 1102 3 1.581840859550752 1102 4 1.6494324928806243 1102 5 1.137004808335302 1102 6 0.527106144952814 1102 7 0.36425437957865725 1102 8 1.842799614543761 1102 9 -3.6685729507983247 1102 10 -0.8231908498176281 1102 11 -0.12245384815785343 1102 12 -4.287549875073797 1102 13 -6.454200126384297 1102 14 -6.316633579410041 1102 15 -1.2720864109476189 1102 16 0.21497961880202254 1102 17 -4.455476573443512 1102 18 -1.778003560437078 1102 19 -1.0218574311681827 1102 20 -0.5164892343125977 1102 21 -0.3913749778250093 1102 22 -0.35005712953206825 1102 23 -0.10089882977584352 1102 24 -0.11850176428804104 1102 25 0.08587761597996035 1103 1 -0.9674950036613055 1103 2 0.8914813530138623 1103 3 1.7353350119587674 1103 4 2.3822252970568982 1103 5 2.159736088706641 1103 6 1.3504553218674538 1103 7 1.280985376585818 1103 8 2.745668598645654 1103 9 3.945686209824437 1103 10 2.245492529497227 1103 11 1.8458718542553392 1103 12 3.1012687809230286 1103 13 3.192493254497503 1103 14 -2.7043017956137803 1103 15 1.6423521511011607 1103 16 2.0759695668800724 1103 17 2.0751483696725366 1103 18 1.797311228702128 1103 19 1.4528805898927182 1103 20 1.4807379877895563 1103 21 1.3371218878106426 1103 22 1.3716882874589622 1103 23 1.7037343309013409 1103 24 1.356236538260454 1103 25 1.2881511287168053 1104 1 1.6029436073674717 1104 2 0.9933490855137812 1104 3 1.6355186646648932 1104 4 1.8885760356732355 1104 5 1.1733496334441966 1104 6 0.5055578053847753 1104 7 0.31472242376273485 1104 8 1.7267200879949982 1104 9 -3.719126197047416 1104 10 -0.9656147666422612 1104 11 -0.25718232329296953 1104 12 1.3205527656860632 1104 13 1.5500156428038139 1104 14 1.320039135006752 1104 15 -3.8799798277016713 1104 16 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2.1338983019176965 1106 4 2.39830850344093 1106 5 1.578296981946199 1106 6 1.0718101042004555 1106 7 0.9700085806471925 1106 8 2.612610164469533 1106 9 3.5594285798581176 1106 10 1.7437359780865407 1106 11 0.9297016790081614 1106 12 2.0074500823365398 1106 13 -4.173047788300153 1106 14 0.30429517418978946 1106 15 2.351728571838926 1106 16 1.770679329208381 1106 17 1.4517433388474927 1106 18 1.1586749632991058 1106 19 1.0515515917654668 1106 20 0.6293015810094573 1106 21 0.21166981348322622 1106 22 0.3046323379798146 1106 23 0.6743430433529234 1106 24 0.6441628186458459 1106 25 0.6216000098019201 1107 1 2.1185149273991826 1107 2 1.4487740656934847 1107 3 2.2668611593794807 1107 4 2.1379979437070595 1107 5 1.7801686411080446 1107 6 0.7483275265792777 1107 7 0.3701118925788016 1107 8 2.2153440639463717 1107 9 3.559092949358501 1107 10 2.024239719351396 1107 11 0.9148165535569248 1107 12 2.5029820732970585 1107 13 2.762997110827069 1107 14 -3.062331537561956 1107 15 1.4100902040108032 1107 16 1.570820301258017 1107 17 1.330635454785373 1107 18 1.4115088180734752 1107 19 1.0354392545688 1107 20 1.1068522477554552 1107 21 0.6734992896271699 1107 22 0.4935156661180429 1107 23 0.9848290299979965 1107 24 1.2581141614047837 1107 25 1.2532212337860322 1108 1 1.5682069626797244 1108 2 1.0736517686295244 1108 3 1.3864320947599786 1108 4 1.7502304184607098 1108 5 1.105062840238901 1108 6 0.2898748120067509 1108 7 0.05248590615863691 1108 8 -4.529986292966665 1108 9 -5.670860286174715 1108 10 -2.173934310744741 1108 11 -0.47885950514888265 1108 12 0.6338355747534941 1108 13 1.9420396437953293 1108 14 2.2112220256697483 1108 15 -3.6032260995652976 1108 16 -0.06509450223013857 1108 17 0.5387060287376606 1108 18 0.4078883021427515 1108 19 0.24086732043453551 1108 20 0.14706894514888447 1108 21 -0.1347287924867473 1108 22 -0.11827222914714142 1108 23 0.1593684570431222 1108 24 0.09648677726558219 1108 25 0.5223815204940543 1109 1 0.7265912854636873 1109 2 0.44093410037487146 1109 3 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1.6177849256231553 1110 15 2.435873384664781 1110 16 1.9102447413427939 1110 17 1.270472696819315 1110 18 0.7624088864007277 1110 19 0.041525673198002266 1110 20 0.07774783082830083 1110 21 -0.13801390326025142 1110 22 -0.35947180994696026 1110 23 0.06243837917070394 1110 24 0.20730523017391178 1110 25 0.44081137359095024 1111 1 1.274466705846212 1111 2 1.1401035962138764 1111 3 1.3115140768740332 1111 4 2.0458462797583614 1111 5 -1.4767577529703217 1111 6 -0.1050947577431458 1111 7 -0.428875162965646 1111 8 1.9056980285877891 1111 9 3.4573912315711155 1111 10 2.0218244957594287 1111 11 1.6062989047247136 1111 12 2.7324735163821714 1111 13 3.007537710352622 1111 14 3.3001505769551187 1111 15 3.6176325212716733 1111 16 2.6413317674927264 1111 17 2.0307576071665636 1111 18 1.4222266351385713 1111 19 1.0181656074610963 1111 20 1.1592484794117723 1111 21 0.8287596652899971 1111 22 0.8216107918943827 1111 23 1.1296251289841202 1111 24 1.0123322846318565 1111 25 1.0222439874006177 1112 1 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13 3.458263667229101 1203 14 3.3450419922429084 1203 15 4.304785389121733 1203 16 3.50563677746759 1203 17 2.7001180463384316 1203 18 2.3721100944701625 1203 19 2.0201755383164617 1203 20 2.028963230781386 1203 21 1.8125747373684113 1203 22 1.531222829977974 1203 23 2.099556459161324 1203 24 1.962158033364151 1203 25 2.2672027059859765 1204 1 0.5731862013497455 1204 2 0.6800210294307121 1204 3 -2.179408742833271 1204 4 0.2940536555376454 1204 5 -2.3734124512674732 1204 6 -0.9930626116392389 1204 7 -0.6829740277810039 1204 8 1.809125308069735 1204 9 3.223963029959851 1204 10 1.779132901668202 1204 11 1.006006526968406 1204 12 2.0707241646487375 1204 13 2.209473119780455 1204 14 -6.021943496650113 1204 15 0.1001267893448059 1204 16 -3.883566641375552 1204 17 -1.1206532265493827 1204 18 0.1386940784215831 1204 19 0.28375941770426394 1204 20 0.061114801559337906 1204 21 0.40659592505624076 1204 22 0.43231048527431426 1204 23 0.5921036870635419 1204 24 -0.01365949924420562 1204 25 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2.0552851673487353 2204 5 2.409332566920476 2204 6 2.2345754157872655 2204 7 2.2239834787111477 2204 8 3.9252339392642455 2204 9 -2.0634971253063767 2204 10 0.9112252952717199 2204 11 2.114633413520668 2204 12 3.8635968502450027 2204 13 4.045734128704126 2204 14 4.158200351942845 2204 15 4.703760598072108 2204 16 4.203401290993856 2204 17 3.3524803973076516 2204 18 3.1109757804181477 2204 19 2.5521298661068634 2204 20 2.4920882729084752 2204 21 2.17100757736873 2204 22 2.0174741582203737 2204 23 2.401912193699193 2204 24 2.452077020490112 2204 25 2.395246388310042 2205 1 -3.0157791336006134 2205 2 -3.576372175051212 2205 3 -0.08128087541184215 2205 4 -1.7726629879730815 2205 5 -2.5322184267210694 2205 6 -0.8623171903511029 2205 7 -0.6515799070569415 2205 8 -4.530366727287216 2205 9 1.0635504655591355 2205 10 0.4874620424996152 2205 11 0.37852570419406817 2205 12 2.0929696353584184 2205 13 -4.138278644124494 2205 14 -0.04196999548343072 2205 15 2.3289118966895117 2205 16 1.785589538483336 2205 17 1.7832455225857409 2205 18 0.4061446041224528 2205 19 0.6107219250758469 2205 20 0.5828668342286463 2205 21 0.5300300672370319 2205 22 0.6296090702632711 2205 23 0.7840343147615982 2205 24 0.44537270472351054 2205 25 0.9860510201080963 2206 1 -6.792631660477294 2206 2 -5.30504134128326 2206 3 -2.118063068603746 2206 4 -0.754166919998621 2206 5 -0.42196155332580787 2206 6 -1.0400648199704747 2206 7 -1.5746823554196752 2206 8 -2.849321276312871 2206 9 -5.764549468674286 2206 10 -2.4259455408468504 2206 11 -1.6430161999695692 2206 12 -7.39814497588327 2206 13 -6.2829515881816995 2206 14 -7.4400836343976415 2206 15 -2.104471485848137 2206 16 -6.021449404231877 2206 17 -2.5294960739984624 2206 18 -1.7195255414385322 2206 19 -1.8100531444018415 2206 20 -1.8526377207920106 2206 21 -1.6788227176164447 2206 22 -1.7325970864553462 2206 23 -1.3013741486142791 2206 24 -1.2375313303984967 2206 25 -1.503090122485414 2207 1 -7.1208320601917805 2207 2 -5.265786531061387 2207 3 -1.1063936933390934 2207 4 -0.23025564169916635 2207 5 0.2613208481034057 2207 6 -0.20934892073695366 2207 7 -0.1679998702046106 2207 8 1.4106931161687477 2207 9 -1.8373011675802786 2207 10 -0.7401999894652533 2207 11 -0.5376542375495248 2207 12 -4.372454276762436 2207 13 -4.84969244216834 2207 14 -1.2269027964356738 2207 15 -5.134727942991358 2207 16 -1.1894420829890606 2207 17 -0.2998587616159263 2207 18 -0.15127411383466693 2207 19 -0.3627159459144451 2207 20 -0.4045198569038 2207 21 -0.5099434136891929 2207 22 -0.48434734677666924 2207 23 -0.5481119213412562 2207 24 -0.6025493992926485 2207 25 -0.11348653410508192 2208 1 -3.7133245428850543 2208 2 -4.293050062213492 2208 3 -1.3080419413957145 2208 4 -0.17667017644797767 2208 5 -2.875770473879033 2208 6 -1.0502068726455285 2208 7 -0.5295247353281738 2208 8 -5.101124766802979 2208 9 -8.112584214724183 2208 10 -3.8488021088139774 2208 11 -1.3698006945050878 2208 12 0.10994670508000226 2208 13 1.0591736289149267 2208 14 1.0769086110168544 2208 15 1.4996129753286311 2208 16 -4.9341740462872 2208 17 -7.034101302320572 2208 18 -2.8368398576371163 2208 19 -2.30535128160691 2208 20 -1.2984852845442845 2208 21 -1.2080131457733465 2208 22 -0.9039124669568928 2208 23 -0.6962110216815915 2208 24 -0.5642009225745461 2208 25 -0.3487934625963973 2209 1 0.7644048662250569 2209 2 0.7655018302856433 2209 3 -2.423708348815925 2209 4 0.19135429353704225 2209 5 0.4739789812233184 2209 6 0.12431766215110684 2209 7 0.18569465198603297 2209 8 1.716963914688744 2209 9 -3.6557201846900043 2209 10 -0.5705778510269823 2209 11 0.05823554958985255 2209 12 -4.349823465734605 2209 13 0.2693725765663437 2209 14 1.4120304448494778 2209 15 2.326140008973063 2209 16 -3.020984282681564 2209 17 -0.23696657577757563 2209 18 0.2286781431886311 2209 19 0.17894642454203336 2209 20 0.2890553427361233 2209 21 0.184179515151488 2209 22 0.033036934375825666 2209 23 0.588695733608958 2209 24 0.10592782449693061 2209 25 0.1984645260351634 2210 1 -0.38389340734873656 2210 2 -3.3641993789678644 2210 3 -1.0155240468673377 2210 4 -3.384588151607826 2210 5 -3.5366599957957057 2210 6 -1.4944395121238294 2210 7 -0.767541148076436 2210 8 -5.1932705070093235 2210 9 -4.53966071088594 2210 10 -1.769342386827521 2210 11 -0.7438855449832182 2210 12 0.9011264885066979 2210 13 -7.169430267801346 2210 14 -1.5198037409230167 2210 15 -5.256420416002776 2210 16 -1.3936899155982019 2210 17 -0.7234512877031705 2210 18 -0.2487170561414267 2210 19 -0.6511708816559469 2210 20 -0.4711945078887467 2210 21 -0.7152425411316159 2210 22 -0.45483244965177505 2210 23 -0.13974526765944972 2210 24 -0.16842897643259747 2210 25 -0.08349340506985214 2211 1 0.4377405482179846 2211 2 0.2471705959771313 2211 3 -3.406728371079158 2211 4 -2.807468805577887 2211 5 -0.6605118861202861 2211 6 -0.8103443380494362 2211 7 -1.0258891727092525 2211 8 -4.927946827576779 2211 9 0.3865224133101819 2211 10 0.11927579005011424 2211 11 -5.784135638718894 2211 12 -0.42459505888816906 2211 13 0.7253242372295183 2211 14 1.3302472238577714 2211 15 -4.2446554254670765 2211 16 -0.7937735605004299 2211 17 -6.081054503900461 2211 18 -2.110649085423546 2211 19 -1.2057273339046652 2211 20 -0.6358988844116666 2211 21 -0.7265502536843194 2211 22 -0.5011675734840217 2211 23 -0.3034530465619447 2211 24 -0.21097042251688336 2211 25 -0.045404847593225384 2212 1 2.2346019073563177 2212 2 1.4275717565467332 2212 3 2.3710910192781043 2212 4 2.6712322882554105 2212 5 -0.8819612359122004 2212 6 0.44062045799132576 2212 7 1.0978075110815695 2212 8 -1.3683029566270513 2212 9 2.9081144208774212 2212 10 2.1882845824946706 2212 11 1.896878185849996 2212 12 3.3621359850593997 2212 13 3.4344013652860887 2212 14 3.7676091339015834 2212 15 4.305453011988632 2212 16 3.4325324489964864 2212 17 2.8262361489509154 2212 18 2.518608993834331 2212 19 2.143399639942711 2212 20 1.9320035217838551 2212 21 1.5264915970511872 2212 22 1.6570204252950198 2212 23 1.7675378358493639 2212 24 1.736293274187659 2212 25 2.2738863784426044 2213 1 2.5133714592789644 2213 2 2.0507174271529713 2213 3 2.599828273513623 2213 4 2.9804759786897 2213 5 1.8353598755351896 2213 6 -0.11215735675119021 2213 7 1.0489105260329161 2213 8 -5.6889932094565125 2213 9 1.1462803289665828 2213 10 1.2884730945290563 2213 11 1.319780190316937 2213 12 3.0693038659262086 2213 13 -1.7585750465570555 2213 14 1.4393674527077742 2213 15 -3.7380878025983897 2213 16 0.3185876412734281 2213 17 1.4392465355273494 2213 18 1.7501339456238334 2213 19 1.5583116446672491 2213 20 1.9794717009418719 2213 21 1.6337394519805493 2213 22 1.6800650580412437 2213 23 2.022613016636445 2213 24 2.0759218994163504 2213 25 2.361353634635151 2214 1 2.093331465756073 2214 2 1.2870536026178918 2214 3 0.6505795887717554 2214 4 1.7030234172661771 2214 5 1.5220789229627485 2214 6 0.16703296160544667 2214 7 0.6245562608865534 2214 8 2.405572105568357 2214 9 3.4298849104574316 2214 10 1.7017159763737553 2214 11 1.710074635272381 2214 12 2.787947321620493 2214 13 1.2877390491183363 2214 14 2.6562323999210378 2214 15 3.7017070622751795 2214 16 2.5722664121512793 2214 17 1.7977023892753614 2214 18 1.591442025631217 2214 19 1.3175484182477994 2214 20 0.6103275404538024 2214 21 0.5637441619042534 2214 22 0.6493185158755601 2214 23 -5.897184701615828 2214 24 -2.225981744819709 2214 25 -0.8434821335453933 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 306327.0 3 4.0 4 2351.0 5 14605.0 6 20912.0 7 19238.0 8 16396.0 9 13519.0 10 11955.0 11 11043.0 12 10135.0 13 9348.0 14 9206.0 15 9110.0 16 8962.0 17 9593.0 18 11992.0 19 16760.0 20 24279.0 21 33454.0 22 42832.0 23 52624.0 24 62686.0 25 71501.0 26 81308.0 27 89952.0 28 96999.0 29 110891.0 30 119319.0 31 130325.0 32 162439.0 33 196898.0 34 231802.0 35 261683.0 36 285601.0 37 274728.0 38 247898.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.905450685283895 18.904555208580945 15.346549484042605 21.84344462209255 2 14.61081900531327 21.624658252499906 41.35210698833947 22.412415753847355 3 15.83945623428564 29.43881007282344 31.0038300699574 23.71790362293352 4 9.373879716596816 17.02045352331167 39.836267525306184 33.76939923478533 5 10.755844158382237 40.65985107407959 34.42178262261667 14.162522144921514 6 28.68746674607966 37.302093355667154 20.175277687543964 13.835162210709221 7 27.9393331784591 34.231126006261654 21.40675762106663 16.422783194212613 8 25.142756605119885 33.339328361369866 23.358551549095697 18.159363484414556 9 26.50989904624921 15.787868409827716 23.41481169865659 34.28742084526649 10 14.944454490804324 32.16403479190188 31.326070629711268 21.565440087582534 11 33.08712724116348 24.776800041966577 23.4305135464397 18.705559170430245 12 22.426419197397493 28.339243431417177 30.50923725033325 18.725100120852083 13 27.192848980857914 23.23647869522704 27.590652767699726 21.98001955621532 14 21.52541875869529 22.844463848767916 29.695685180780597 25.934432211756196 15 22.495051763038504 29.312329458911307 26.698063875019347 21.494554903030846 16 22.43563636411028 29.728606979925075 26.27239914479399 21.563357511170654 17 20.895024913390415 29.45146230344845 27.361559060520985 22.29195372264015 18 20.850556534618594 28.340893785181514 29.386878470094267 21.421671210105625 19 22.781506921612397 27.952713066164176 28.792128791640113 20.47365122058331 20 23.908511507931077 27.02735737068856 28.2573536745394 20.806777446840965 21 25.238188170202037 26.5874134212921 27.115087819211897 21.05931058929397 22 24.00995179842004 27.124808663121332 27.250444253442435 21.614795285016196 23 22.609286968977074 27.429014748197574 27.785118080398092 22.176580202427264 24 22.350138594775725 27.652679895432385 28.275940943995458 21.721240565796432 25 22.225321209486943 26.997888308082196 28.703370333286305 22.073420149144553 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 11272.0 1 11272.0 2 9078.5 3 6885.0 4 6885.0 5 6885.0 6 9307.5 7 11730.0 8 11730.0 9 11730.0 10 12478.0 11 13226.0 12 13226.0 13 13226.0 14 16974.5 15 20723.0 16 20723.0 17 20723.0 18 33542.0 19 46361.0 20 46361.0 21 46361.0 22 70316.0 23 94271.0 24 94271.0 25 94271.0 26 133286.0 27 172301.0 28 172301.0 29 172301.0 30 207883.5 31 243466.0 32 243466.0 33 243466.0 34 273066.5 35 302667.0 36 302667.0 37 302667.0 38 326483.5 39 350300.0 40 350300.0 41 350300.0 42 369704.5 43 389109.0 44 389109.0 45 389109.0 46 393195.0 47 397281.0 48 397281.0 49 397281.0 50 370240.5 51 343200.0 52 343200.0 53 343200.0 54 302493.0 55 261786.0 56 261786.0 57 261786.0 58 221206.0 59 180626.0 60 180626.0 61 180626.0 62 146025.0 63 111424.0 64 111424.0 65 111424.0 66 85779.5 67 60135.0 68 60135.0 69 60135.0 70 45812.5 71 31490.0 72 31490.0 73 31490.0 74 23206.0 75 14922.0 76 14922.0 77 14922.0 78 11739.5 79 8557.0 80 8557.0 81 8557.0 82 6369.0 83 4181.0 84 4181.0 85 4181.0 86 3070.0 87 1959.0 88 1959.0 89 1959.0 90 1320.5 91 682.0 92 682.0 93 682.0 94 389.5 95 97.0 96 97.0 97 97.0 98 60.5 99 24.0 100 24.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24962037240046447 2 0.2618009370914436 3 0.26748520061390046 4 0.3330978424159744 5 0.2779767270010638 6 0.3081845274346919 7 0.3091589726099702 8 0.3074374528003118 9 0.31974794351466135 10 0.31084801091378594 11 0.3107180848904155 12 0.3129268272877131 13 0.3109779369371564 14 0.308087082917164 15 0.3053261549205421 16 0.3076973048470527 17 0.30669037816593175 18 0.30578089600233865 19 0.2974981120124729 20 0.3448561475309996 21 0.30396193167515245 22 0.3079571568937936 23 0.3125370492176017 24 0.31364142041625054 25 0.3109129739254712 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3078675.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.9190974889478 #Duplication Level Percentage of deduplicated Percentage of total 1 92.4245461700837 69.24363584888256 2 4.26341286355637 6.3882208792082755 3 1.3142129568245897 2.9537894590073934 4 0.605325083807463 1.8140163586510722 5 0.3501107637956469 1.3114991222368022 6 0.2091702806186202 0.9402509187274184 7 0.14958080938080243 0.7844521468333236 8 0.10020408382755214 0.6005759620053661 9 0.0791469491793165 0.533665620136621 >10 0.4266881687201499 5.896013994299792 >50 0.043106369417741465 2.265425611924137 >100 0.0315330041707896 4.460278266389737 >500 0.0019044621115420308 1.0154101459984168 >1k 9.31070364991192E-4 1.1864366036714762 >5k 1.2696414068061708E-4 0.60632906202757 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7081 0.23000154287152755 No Hit NNNNNNNNNNNNNNNNNNNNNNNNN 6950 0.2257464656061455 No Hit TATCAACGCAGAGTACTTTTTTTTT 5092 0.16539582775057451 No Hit GGTATCAACGCAGAGTACTTTTTTT 3926 0.12752239193809026 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 3186 0.10348607761455822 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 6.496301168522173E-5 9 0.0 0.0 0.0 0.0 6.496301168522173E-5 10 0.0 0.0 0.0 0.0 6.496301168522173E-5 11 0.0 0.0 0.0 0.0 6.496301168522173E-5 12 0.0 0.0 0.0 3.2481505842610864E-5 6.496301168522173E-5 13 0.0 3.2481505842610864E-5 0.0 3.2481505842610864E-5 6.496301168522173E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGACGG 85 1.7149432E-8 14.531794 14 GGTATCA 3685 0.0 13.848263 1 ACATGGT 2110 0.0 12.477472 3 CTCAACG 320 0.0 12.1774435 4 ATAACGC 125 1.4024408E-9 12.165763 3 TAACGCA 150 1.4551915E-11 12.038839 4 TCGACCG 80 2.866077E-5 11.875978 18 GTATCAA 9785 0.0 11.556988 1 CATGGTA 1865 0.0 11.160601 4 TACATGT 2310 0.0 10.943506 2 TATAACG 150 2.1227606E-9 10.770715 2 CTCGACG 120 1.5314217E-6 10.2883215 13 CGCGCCA 130 3.8597682E-7 10.227943 10 CAACGCG 235 0.0 10.103921 6 GTACTAG 160 6.148184E-9 10.096886 1 ACATGTG 2100 0.0 9.957097 3 CGTCACT 210 5.456968E-12 9.955473 1 TCAACTC 2200 0.0 9.677153 4 CAACTCA 2200 0.0 9.670371 5 TTTACGC 90 0.0011093478 9.5045 3 >>END_MODULE