FastQCFastQC Report
Thu 26 May 2016
SRR1364277_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364277_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3078675
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT240180.7801408073278276No Hit
TATCAACGCAGAGTACTTTTTTTTT199600.6483308566185129No Hit
GGTATCAACGCAGAGTACTTTTTTT155310.5044702672415893No Hit
ACGCAGAGTACTTTTTTTTTTTTTT79150.257091118744265No Hit
GTACTTTTTTTTTTTTTTTTTTTTT78750.2557918585105606No Hit
GTGGTATCAACGCAGAGTACATGGG58100.1887175489455691No Hit
GAGTACTTTTTTTTTTTTTTTTTTT56800.1844949531860297No Hit
GGTATCAACGCAGAGTACATGGGAT49360.16032871283912722No Hit
GGTATCAACGCAGAGTACATGGGAG36200.11758305115025132No Hit
GGTATCAACGCAGAGTACATGGGAA32640.10601963507028186No Hit
GGTCGGGAGTGGGTAATTTGCGCGC32040.1040707447197252No Hit
ATCAACGCAGAGTACTTTTTTTTTT31370.10189448382827027No Hit
GCAGAGTACTTTTTTTTTTTTTTTT31070.10092003865299194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTA504.6820005E-617.054719
GGTATCA62200.013.7895481
TATAACG1950.013.1256182
ATAACGC2050.012.4841283
TGCACCG701.1141853E-412.187485
TCGACCG1103.5508492E-812.1542418
CGACCGT1103.5517587E-812.15404119
AGGACCG1650.012.0643745
AAACGCA2000.011.8489395
TCGACGG1052.546676E-711.82273914
TAACGCA2200.011.6331254
GCGTACA1501.8553692E-1011.36943813
ATAAACG2200.011.2020873
TAAACGC2800.011.1714944
ACCGTCC855.4488875E-511.151588
GGTCGAA1303.0266165E-811.01931615
AGGATCG700.001519717410.8333165
GTCGAAA1355.2712494E-810.61119216
AAGTTCG1355.278889E-810.61014914
AACGGAC1451.3316821E-810.53801215
Produced by FastQC (version 0.11.5)