FastQCFastQC Report
Thu 26 May 2016
SRR1364276_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364276_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2984283
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66760.22370532553380496No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN65390.21911460809849467No Hit
TATCAACGCAGAGTACTTTTTTTTT46970.15739123936972466No Hit
GTACTTTTTTTTTTTTTTTTTTTTT38100.12766885714256992No Hit
GGTATCAACGCAGAGTACTTTTTTT36330.12173778425169464No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA32340.10836773858243337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31150.013.5447771
CGCGGGT655.4463897E-513.15625615
TATAACG1750.013.0327112
TAACGCA1501.8189894E-1212.671334
ACATGGT17900.012.4766593
ACGGCCC1457.2759576E-1212.45055615
TACATGT23800.012.41780952
ATAACGC1551.8189894E-1212.2625783
GCCCCCG1701.8189894E-1211.73704918
AACGTAG1001.9376403E-611.39691455
CATGGTA12250.011.2490394
CTCAACG2550.011.1805864
ACGCGTC855.3155833E-511.17870514
TTAGACG600.00586171411.0874153
GTATCAA79150.010.961421
ACATGTG21250.010.8228073
CAACGTA803.760046E-410.6914354
CATGTGA14500.010.5521594
GCGGGTA1002.395105E-510.4520916
TTAGACT4250.010.2861394
Produced by FastQC (version 0.11.5)