##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364276_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2984283 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.92290007348499 30.0 24.0 31.0 7.0 34.0 2 25.58075524338677 30.0 25.0 31.0 7.0 34.0 3 25.17701437832806 30.0 24.0 31.0 7.0 34.0 4 28.233622615549532 33.0 26.0 35.0 7.0 37.0 5 28.7978010128396 35.0 28.0 35.0 7.0 37.0 6 29.369165390815816 35.0 29.0 36.0 2.0 37.0 7 29.30706337167085 35.0 29.0 36.0 2.0 37.0 8 27.641298429136913 33.0 25.0 35.0 2.0 37.0 9 27.482869084466856 33.0 17.0 37.0 2.0 39.0 10 29.185994759880348 34.0 27.0 38.0 2.0 39.0 11 29.643941945184153 35.0 28.0 39.0 2.0 39.0 12 28.275596851907142 34.0 23.0 39.0 2.0 39.0 13 28.05529502396388 34.0 22.0 38.0 2.0 39.0 14 28.568731584772625 34.0 22.0 39.0 2.0 41.0 15 28.131230181588005 34.0 18.0 39.0 2.0 40.0 16 28.921544639030547 34.0 25.0 39.0 2.0 41.0 17 29.461662650626632 35.0 27.0 39.0 2.0 41.0 18 29.997911056022502 36.0 27.0 40.0 2.0 41.0 19 30.637435189625112 36.0 29.0 40.0 2.0 41.0 20 30.664971787193103 37.0 29.0 40.0 2.0 41.0 21 30.83656141190363 37.0 30.0 40.0 2.0 41.0 22 30.72572339821659 37.0 30.0 40.0 2.0 41.0 23 30.366261175632474 37.0 29.0 40.0 2.0 41.0 24 30.339067709061105 37.0 29.0 40.0 2.0 41.0 25 30.064218440409306 36.0 29.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.34133354296078977 1101 2 -0.45703261680825946 1101 3 -2.8523046824220266 1101 4 -2.8637556822017807 1101 5 -3.467308498729782 1101 6 -1.564057239370122 1101 7 -1.1027264653099742 1101 8 -4.6304831409549365 1101 9 -5.86715673049272 1101 10 -5.898477575322168 1101 11 -2.906273535941466 1101 12 -0.42864308556194786 1101 13 0.029814732095186258 1101 14 -7.055978504029127 1101 15 -9.404876511886808 1101 16 -4.262059410099329 1101 17 -2.2070579164837767 1101 18 -6.8737096265652795 1101 19 -3.3931084640148015 1101 20 -2.193639041924765 1101 21 -2.036498559583645 1101 22 -1.9771694101184032 1101 23 -6.848001955488133 1101 24 -3.416364860076264 1101 25 -2.4548295391011017 1102 1 1.8920098132648278 1102 2 1.3526948674324117 1102 3 1.7567620927591534 1102 4 1.933826462082429 1102 5 1.3792382139620045 1102 6 0.8964357265640714 1102 7 0.6913152593224225 1102 8 2.0478191013665246 1102 9 -3.1507881446426396 1102 10 -0.42320699064008593 1102 11 0.3305053336633783 1102 12 -4.042387076838022 1102 13 -6.080158379825772 1102 14 -5.9368011028448215 1102 15 -0.8846505825308242 1102 16 0.5719473947763376 1102 17 -3.9959873646767825 1102 18 -1.263597730995798 1102 19 -0.4792464698868777 1102 20 -0.11820054038725303 1102 21 0.08014104527385157 1102 22 0.047065074778434024 1102 23 0.325008400164176 1102 24 0.423121259554307 1102 25 0.5395462151215398 1103 1 -0.8914861962632052 1103 2 0.910627166781186 1103 3 1.921043712305888 1103 4 2.625387181194153 1103 5 2.2526886362228993 1103 6 1.5642121228285646 1103 7 1.4560644051371625 1103 8 2.8078914818884435 1103 9 3.961879628748399 1103 10 2.299781808951309 1103 11 1.9118358511672113 1103 12 3.177096260789444 1103 13 3.371262317148794 1103 14 -2.389107105856194 1103 15 1.8453854708254163 1103 16 2.329018156082764 1103 17 2.2950343639048754 1103 18 2.1159902298839093 1103 19 1.6338678466199994 1103 20 1.6537079541029662 1103 21 1.456776338722097 1103 22 1.6089766933675023 1103 23 1.9625858865641987 1103 24 1.7544427947730767 1103 25 1.7430730075081442 1104 1 1.7862585767146975 1104 2 1.2413384125949776 1104 3 1.883949447974949 1104 4 2.21057742840285 1104 5 1.3728504014175833 1104 6 0.8433947137775242 1104 7 0.7496666477965448 1104 8 2.1276258618612083 1104 9 -3.1911790887930245 1104 10 -0.36516529442771173 1104 11 0.4638884457947796 1104 12 1.902136190295174 1104 13 2.189633864185513 1104 14 1.8227002571111193 1104 15 -3.3542295307760313 1104 16 -4.145694952718316 1104 17 -1.0244267887748002 1104 18 -0.3148842306116215 1104 19 0.1674621486111718 1104 20 0.5192578651973676 1104 21 0.0474765680276974 1104 22 0.04715195383554871 1104 23 -0.12658033615894482 1104 24 -6.345010211911333 1104 25 -3.2022832618740438 1105 1 -2.113797714524871 1105 2 0.07318836817301033 1105 3 1.1590463159626836 1105 4 1.4966385165128635 1105 5 1.2222883669264704 1105 6 1.0712329967366934 1105 7 0.3983258768558855 1105 8 2.660746882758847 1105 9 3.020240939370151 1105 10 1.721687733816232 1105 11 1.5457249518751475 1105 12 2.2908548008180887 1105 13 2.2543681224790753 1105 14 2.870394317911277 1105 15 3.31537987193283 1105 16 2.746247288185714 1105 17 1.6332785443197437 1105 18 1.803205519326049 1105 19 0.8015854906884314 1105 20 1.033805024473832 1105 21 1.0204062759664119 1105 22 0.9659687140868414 1105 23 1.4737456473575214 1105 24 1.5029543137217232 1105 25 1.5507710382780076 1106 1 2.26355340631714 1106 2 1.5399386060791471 1106 3 2.1643436449743554 1106 4 2.3920187012568164 1106 5 1.511268125228142 1106 6 0.9892192733088407 1106 7 1.1518139028431058 1106 8 2.5827497304774525 1106 9 3.672364707803407 1106 10 1.8042540434448142 1106 11 1.0040430806807485 1106 12 2.0689889727258475 1106 13 -3.947082139038802 1106 14 0.5018579594135559 1106 15 2.479724717776808 1106 16 2.0572782624918524 1106 17 1.6788942024790288 1106 18 1.4223968531029954 1106 19 1.0947521823978512 1106 20 0.8984452316847147 1106 21 0.4369088768350373 1106 22 0.5436043205865104 1106 23 0.9022352116851948 1106 24 0.8423108581082097 1106 25 0.7807287518032417 1107 1 1.9379010118631719 1107 2 1.3260684769584437 1107 3 2.161669213565151 1107 4 2.158088904800394 1107 5 1.6521993114769167 1107 6 0.9508759092719075 1107 7 0.44181523456735405 1107 8 2.128971580947802 1107 9 3.4879537761234687 1107 10 1.7870479052182162 1107 11 0.8374600001901626 1107 12 2.4342579886352773 1107 13 2.7633239121653794 1107 14 -3.117360575810114 1107 15 1.3390558529314482 1107 16 1.5773358999811435 1107 17 1.3415841462646085 1107 18 1.3914328145147472 1107 19 1.032759983822615 1107 20 1.1216890828630426 1107 21 0.7026982003792916 1107 22 0.5322015495416608 1107 23 1.1081068034552395 1107 24 1.2568760718905487 1107 25 1.307142019691426 1108 1 1.8331464244758813 1108 2 1.1376541896304495 1108 3 1.4208304307868573 1108 4 1.7917557947546712 1108 5 1.0684670842571435 1108 6 0.4072054121368822 1108 7 0.20848018458292472 1108 8 -3.8914168480072995 1108 9 -5.354818518987152 1108 10 -1.932502387285112 1108 11 -0.4820781109766692 1108 12 0.6550132447786794 1108 13 1.8945356836305223 1108 14 2.189591174956348 1108 15 -3.7164829624066478 1108 16 -0.21594040069378906 1108 17 0.4091898628898285 1108 18 0.37608042010741727 1108 19 0.21266091141183807 1108 20 0.15185618851772986 1108 21 -0.20870858461775654 1108 22 -0.13037720029021216 1108 23 0.26178982058414846 1108 24 0.23641360313427384 1108 25 0.5382724764930202 1109 1 0.9415643232398203 1109 2 0.7729935606373743 1109 3 1.0485551154666553 1109 4 -1.0203986353461723 1109 5 -2.18548014231283 1109 6 -0.5890478952539908 1109 7 -0.5337666098601446 1109 8 1.6306363572399256 1109 9 -3.455525132972511 1109 10 -0.6157067334190423 1109 11 0.0987598975496553 1109 12 -6.732586198165336 1109 13 -1.4439036275443549 1109 14 0.42377598062459754 1109 15 1.9881688208228887 1109 16 1.4923666384427428 1109 17 1.1303397767549832 1109 18 0.7843685967371705 1109 19 0.29334524728716005 1109 20 0.22094989004797938 1109 21 0.1718119615779088 1109 22 0.04293376841233254 1109 23 0.3216510698130186 1109 24 0.17858541948159257 1109 25 0.4400791182485939 1110 1 0.26446222016173593 1110 2 0.17476844723659113 1110 3 -3.145872463217003 1110 4 -2.6293040554037823 1110 5 -0.6277356608264633 1110 6 -0.6861233190546265 1110 7 -0.7902548721786182 1110 8 -4.558480300522046 1110 9 -8.088978386660148 1110 10 -3.382477485923708 1110 11 -1.359958225540872 1110 12 0.97773905018758 1110 13 1.37985216770036 1110 14 1.5284033818110707 1110 15 2.218567319975005 1110 16 1.6176216405763881 1110 17 1.053448281978774 1110 18 0.628690722088713 1110 19 -0.20788674059534884 1110 20 -0.23001212608020793 1110 21 -0.3697730727937909 1110 22 -0.646795775349716 1110 23 -0.08200151052761484 1110 24 0.0675320391178289 1110 25 0.2637219620311093 1111 1 0.9807248892344695 1111 2 0.8222176209348184 1111 3 1.0497008171272384 1111 4 1.6605644551012944 1111 5 -1.4853214822868317 1111 6 -0.30761156546905255 1111 7 -0.6942795206953427 1111 8 1.481356155036746 1111 9 2.8930565265977997 1111 10 1.4209857957406307 1111 11 1.1726258115801258 1111 12 2.415471922266555 1111 13 2.5191349622263637 1111 14 2.812089866961472 1111 15 3.0913295004861467 1111 16 2.061364420318167 1111 17 1.5333072486367882 1111 18 0.9060253801492841 1111 19 0.5828115140365107 1111 20 0.594105173337411 1111 21 0.34802823370055336 1111 22 0.4281090019847298 1111 23 0.7245203077847364 1111 24 0.462877745134076 1111 25 0.5210035931046768 1112 1 1.9985476029861218 1112 2 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15 4.66207093434646 1203 16 3.9536922214852055 1203 17 3.073832736721805 1203 18 2.76747491606012 1203 19 2.2142681767588854 1203 20 2.1699737640739123 1203 21 2.045953840622193 1203 22 1.8527586286312214 1203 23 2.3985012668402774 1203 24 2.313206027064645 1203 25 2.5422519870328344 1204 1 0.614348967238012 1204 2 0.6099419318408259 1204 3 -2.150064577925459 1204 4 0.3172073672080131 1204 5 -2.137335118470755 1204 6 -0.9686644708966945 1204 7 -0.6213215186954173 1204 8 1.6886258411200181 1204 9 3.0575423971863103 1204 10 1.6804880769972677 1204 11 1.0102078244691874 1204 12 2.0839390700464264 1204 13 2.212950742610019 1204 14 -6.240190777623852 1204 15 0.06353914168859376 1204 16 -3.8238586900560456 1204 17 -1.161655948890477 1204 18 0.07875387058027528 1204 19 0.15606358125165798 1204 20 0.039858653542953704 1204 21 0.24283315660551708 1204 22 0.3010721255032358 1204 23 0.5354420727894862 1204 24 -0.11636234813947155 1204 25 -0.04811798344507068 1205 1 1.2822494011719456 1205 2 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2.0242150879139906 2204 6 1.8076662281056315 2204 7 1.8454257117277102 2204 8 3.492143298665294 2204 9 -1.9945408748741826 2204 10 0.656659716166466 2204 11 1.65292291619663 2204 12 3.52873709961888 2204 13 3.7675691728025633 2204 14 3.823727064723766 2204 15 4.2842829877120465 2204 16 3.8763229493751794 2204 17 2.95526806815505 2204 18 2.6864429790591338 2204 19 2.144645911359234 2204 20 2.1213433964724224 2204 21 1.7622864362219524 2204 22 1.7280103480436892 2204 23 2.240178066620672 2204 24 2.0814946889145958 2204 25 2.1482770613959516 2205 1 -2.7434394325008498 2205 2 -3.265566738542386 2205 3 -0.02745240030735374 2205 4 -1.9382262559541203 2205 5 -2.400666981150671 2205 6 -0.6065211766677052 2205 7 -0.3704253667040973 2205 8 -4.0764116125934 2205 9 1.4466081374201138 2205 10 0.8142798769102448 2205 11 0.7308812045460229 2205 12 2.346706353088635 2205 13 -4.213914448536318 2205 14 0.059961165884146794 2205 15 2.5108076736606506 2205 16 2.1149341914051263 2205 17 1.9973748907319653 2205 18 0.7464038017603158 2205 19 0.8944584475749338 2205 20 0.8169205054708684 2205 21 0.7238572703015898 2205 22 0.8307254891383806 2205 23 1.0086877513071713 2205 24 0.6817294199584971 2205 25 1.1067626555726378 2206 1 -6.9432502863514145 2206 2 -5.442571497328906 2206 3 -2.106140247708602 2206 4 -0.7867330344462609 2206 5 -0.6269210075001723 2206 6 -1.112129380494487 2206 7 -1.565030584256867 2206 8 -2.827698413739782 2206 9 -5.701014064055954 2206 10 -2.5282650870115013 2206 11 -1.9000264887745786 2206 12 -7.773759808998921 2206 13 -6.597213428217941 2206 14 -7.8930619308952 2206 15 -2.4119394898752553 2206 16 -6.310208582438509 2206 17 -3.011304537828181 2206 18 -2.216238665092092 2206 19 -2.198795724512248 2206 20 -2.1497910019914386 2206 21 -2.1222375549823767 2206 22 -2.1294447589367813 2206 23 -1.714517560002431 2206 24 -1.7162357397105623 2206 25 -1.9783784260886108 2207 1 -6.849030816074773 2207 2 -4.980188788320181 2207 3 -0.7895176881046524 2207 4 0.3694201915846733 2207 5 0.601210796509843 2207 6 0.20514712388548162 2207 7 0.21338091258203207 2207 8 1.6605607028031528 2207 9 -1.4969377050562613 2207 10 -0.25008861057902365 2207 11 -0.18732524276131102 2207 12 -4.016688157861463 2207 13 -4.3766448772504525 2207 14 -0.7928915631567754 2207 15 -4.582008884002985 2207 16 -0.7749384706568065 2207 17 0.08797933142477987 2207 18 0.16202784037862727 2207 19 -0.025976161307745826 2207 20 0.06372610854925043 2207 21 -0.1457503986774391 2207 22 -0.0973102511387971 2207 23 -0.14360835818200002 2207 24 -0.2614519338175043 2207 25 0.27417623474613606 2208 1 -3.579343224260665 2208 2 -4.138477984085416 2208 3 -1.3037965290774949 2208 4 -0.05157127724676158 2208 5 -2.7990429176084177 2208 6 -1.1226575137365309 2208 7 -0.7508013144266599 2208 8 -5.005147757323744 2208 9 -8.381919868441805 2208 10 -3.8249499272608283 2208 11 -1.526304833446904 2208 12 0.01185794604382906 2208 13 0.9310016333673197 2208 14 0.9911262946016244 2208 15 1.3527178516483538 2208 16 -4.343154981727473 2208 17 -6.912510145901244 2208 18 -2.716305855964208 2208 19 -2.2349560300642253 2208 20 -1.305179516930302 2208 21 -1.176282284947039 2208 22 -0.908169470051206 2208 23 -0.7080789608612257 2208 24 -0.6650644341560223 2208 25 -0.4801058177310189 2209 1 0.6266081692432159 2209 2 0.5551713904777138 2209 3 -2.438825629598714 2209 4 0.018575829637924812 2209 5 0.2394310276818743 2209 6 -0.08045068199307437 2209 7 -0.06028566385277756 2209 8 1.3469303380978879 2209 9 -3.922530136685797 2209 10 -0.9615012547392894 2209 11 -0.2321387454132342 2209 12 -4.583834342392549 2209 13 -0.11062242637475705 2209 14 0.9580469968816203 2209 15 1.8991307740876913 2209 16 -3.119436459279658 2209 17 -0.5127045467205704 2209 18 -0.040011994506993886 2209 19 -0.09911939297655081 2209 20 0.020204309623505168 2209 21 -0.06564245758000098 2209 22 -0.150211304471771 2209 23 0.2836920335829056 2209 24 -0.22037214605076372 2209 25 0.05336718221037273 2210 1 -0.23467924421601438 2210 2 -2.881689575449059 2210 3 -0.7627105841406525 2210 4 -3.148504686722813 2210 5 -3.3475126883633237 2210 6 -1.5309290470489358 2210 7 -0.7246876530268835 2210 8 -4.882217920383752 2210 9 -4.362228589308135 2210 10 -1.7415478722910365 2210 11 -0.8134191820960162 2210 12 0.908427976473881 2210 13 -7.021405311866431 2210 14 -1.5732079706030362 2210 15 -5.245008436386989 2210 16 -1.3133266265835495 2210 17 -0.7388056188053369 2210 18 -0.27679803936845815 2210 19 -0.6587147399423472 2210 20 -0.4963564170711905 2210 21 -0.7230288223425241 2210 22 -0.4928378229612207 2210 23 -0.13754273692673635 2210 24 -0.27110341925300574 2210 25 -0.10542266420144486 2211 1 0.3320953819387107 2211 2 0.27817199742388965 2211 3 -3.436300726954123 2211 4 -2.864463938929859 2211 5 -0.798721156947682 2211 6 -0.7075904129256116 2211 7 -0.8495875905875288 2211 8 -4.68392568529427 2211 9 0.5087149480491959 2211 10 0.30207116560025327 2211 11 -5.840151417584671 2211 12 -0.3778031709733263 2211 13 0.956961079494409 2211 14 1.4805464556087884 2211 15 -3.764648492722639 2211 16 -0.5595464301966864 2211 17 -5.864463920894536 2211 18 -1.89019868040727 2211 19 -0.9536885709198692 2211 20 -0.4675184106352326 2211 21 -0.5282752086315128 2211 22 -0.16552049818324832 2211 23 -0.03467760351700022 2211 24 -0.0695149041805827 2211 25 0.09372808753954232 2212 1 1.9841631194043892 2212 2 1.2172439634569443 2212 3 2.1013757436906495 2212 4 2.4575074678669893 2212 5 -0.9123211642175235 2212 6 0.4932576772288364 2212 7 0.9366517840381654 2212 8 -1.724177480517458 2212 9 2.556920441359626 2212 10 1.9251450196955453 2212 11 1.681363142461354 2212 12 3.1605665561401644 2212 13 3.2150951518352926 2212 14 3.5999344466312273 2212 15 4.044055816704272 2212 16 3.1416834141185674 2212 17 2.4648209833978 2212 18 2.1963679595042755 2212 19 1.76222670674359 2212 20 1.5434142805751954 2212 21 1.4091210934297855 2212 22 1.350612350517153 2212 23 1.5297848501006115 2212 24 1.6479030641113646 2212 25 1.9612930691927986 2213 1 1.9655780134043184 2213 2 1.500561999666136 2213 3 2.0516924841434445 2213 4 2.386456825387775 2213 5 1.0857172045691428 2213 6 -0.6170525844419714 2213 7 0.5405795456189892 2213 8 -6.370252418429132 2213 9 0.42033062024599843 2213 10 0.5098253997840878 2213 11 0.4319525316561865 2213 12 2.0547914418616493 2213 13 -2.4737928372227636 2213 14 0.5741317975292617 2213 15 -4.100803449753638 2213 16 -0.5135419035217161 2213 17 0.501067968964513 2213 18 0.6917275319046645 2213 19 0.512861987310842 2213 20 0.8774143885548931 2213 21 0.6072476456379725 2213 22 0.5554201466514819 2213 23 0.8719788176347159 2213 24 0.8414017863515859 2213 25 1.2075110802067286 2214 1 2.404728471914492 2214 2 1.6416062934790467 2214 3 1.3292875702985754 2214 4 2.1162666021884817 2214 5 1.957365907407734 2214 6 0.7075598955840867 2214 7 0.9194221763783545 2214 8 2.3725554347661912 2214 9 3.252442871271409 2214 10 1.7782362880012421 2214 11 1.811151385635199 2214 12 2.981831328684443 2214 13 1.4781060869831109 2214 14 2.968513902910267 2214 15 3.9068418638754636 2214 16 2.619678201716802 2214 17 1.9778508661788479 2214 18 1.7017371499315956 2214 19 1.5292329887052034 2214 20 1.1103347573985936 2214 21 0.8938633214951963 2214 22 0.8886626430638067 2214 23 -5.738469972733007 2214 24 -1.930258366519066 2214 25 -0.40812737358094253 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 283598.0 3 8.0 4 2139.0 5 13089.0 6 18949.0 7 17569.0 8 14894.0 9 12178.0 10 10812.0 11 9954.0 12 9349.0 13 8805.0 14 8406.0 15 8250.0 16 8287.0 17 8856.0 18 10903.0 19 14991.0 20 21636.0 21 30353.0 22 38916.0 23 48118.0 24 57214.0 25 66143.0 26 75426.0 27 83462.0 28 90071.0 29 104122.0 30 113451.0 31 122740.0 32 153029.0 33 190008.0 34 227533.0 35 260114.0 36 288488.0 37 284757.0 38 267665.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.3047244107713 18.596701024694447 14.94508705955858 22.153487504975672 2 14.792334855069955 21.842256528579345 41.18777428129608 22.177634335054623 3 15.655488014857832 29.31492499354102 31.35807869504787 23.671508296553274 4 9.411960081515144 17.170690317084762 39.75819773809596 33.65915186330413 5 10.505712113255107 40.41732144039131 35.283895439146114 13.793071007207466 6 28.624415996805773 37.83557602435194 19.948685356337315 13.591322622504972 7 27.600725023215734 33.75290259676751 22.132701565158825 16.513670814857935 8 24.54483797939386 33.891438665548165 24.14318093824611 17.420542416811855 9 25.695172004421995 16.120992482001608 23.977631879244147 34.20620363433225 10 14.726154125375382 31.673189807431505 32.04538694352343 21.555269123669675 11 32.93840431513615 25.036709510393102 23.840092061973785 18.184794112496963 12 21.836135548217424 27.9388302118256 31.382117919891105 18.842916320065875 13 26.589865607669307 23.539881958450533 28.051386825202584 21.818865608677577 14 21.15651473411776 22.546999521762494 29.984785796312497 26.31169994780725 15 21.664446302589692 29.664512174137485 26.96908876796008 21.701952755312742 16 22.105216813826186 29.797046234257873 26.344197326392997 21.753539625522944 17 20.971708683661667 29.521478775967537 27.674756397321897 21.8320561430489 18 20.71699356070281 28.53418608090123 29.463716778469358 21.285103579926602 19 22.469870387975778 28.081205029896893 28.745681307458646 20.70324327466868 20 23.31562301532201 27.35573397741825 28.409309062251943 20.919333945007796 21 24.360656442905384 26.97277681586475 27.554584829974406 21.11198191125546 22 23.527148118348414 27.324967449951572 27.549339286110385 21.59854514558963 23 22.200177863580112 27.634831147233534 28.387625642201126 21.777365346985224 24 22.230807779164387 27.616147852925486 28.365959383332196 21.787084984577927 25 21.918569263649513 27.362980584217787 28.941544275437987 21.77690587669471 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 10784.0 1 10784.0 2 8796.0 3 6808.0 4 6808.0 5 6808.0 6 9798.0 7 12788.0 8 12788.0 9 12788.0 10 14088.0 11 15388.0 12 15388.0 13 15388.0 14 19937.5 15 24487.0 16 24487.0 17 24487.0 18 38599.0 19 52711.0 20 52711.0 21 52711.0 22 76579.5 23 100448.0 24 100448.0 25 100448.0 26 136496.5 27 172545.0 28 172545.0 29 172545.0 30 205070.0 31 237595.0 32 237595.0 33 237595.0 34 265464.5 35 293334.0 36 293334.0 37 293334.0 38 316029.0 39 338724.0 40 338724.0 41 338724.0 42 355028.0 43 371332.0 44 371332.0 45 371332.0 46 374023.5 47 376715.0 48 376715.0 49 376715.0 50 352077.0 51 327439.0 52 327439.0 53 327439.0 54 290117.5 55 252796.0 56 252796.0 57 252796.0 58 214374.5 59 175953.0 60 175953.0 61 175953.0 62 141213.5 63 106474.0 64 106474.0 65 106474.0 66 81589.5 67 56705.0 68 56705.0 69 56705.0 70 42356.5 71 28008.0 72 28008.0 73 28008.0 74 20284.5 75 12561.0 76 12561.0 77 12561.0 78 9366.0 79 6171.0 80 6171.0 81 6171.0 82 4427.0 83 2683.0 84 2683.0 85 2683.0 86 1956.5 87 1230.0 88 1230.0 89 1230.0 90 864.5 91 499.0 92 499.0 93 499.0 94 291.0 95 83.0 96 83.0 97 83.0 98 52.5 99 22.0 100 22.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24454785286784128 2 0.25600789201292234 3 0.2615703671535173 4 0.32238899594978093 5 0.2702156598419118 6 0.2986982132726689 7 0.29923435545489485 8 0.29685522452126695 9 0.3068073637788373 10 0.3008427820015729 11 0.29809505331766456 12 0.3001726042737904 13 0.2978939999993298 14 0.2940069691781912 15 0.29471065579236283 16 0.2957829401568149 17 0.29290117592735004 18 0.2949787268834758 19 0.28670203194536176 20 0.33287727738957734 21 0.29256608706345877 22 0.2970562778396017 23 0.3005412020240708 24 0.3016469952749119 25 0.29889926659100363 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2984283.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.51885481379198 #Duplication Level Percentage of deduplicated Percentage of total 1 92.62485062832933 70.87547497378299 2 4.253857803299303 6.510006552983508 3 1.2669107841943072 2.9082768707337454 4 0.5649636981543669 1.7292150077654795 5 0.3271775942621077 1.2517627416833976 6 0.19110218545638624 0.877375223012134 7 0.12656558103595808 0.6779257323799589 8 0.09558513390151585 0.5851251986693565 9 0.0755282424018103 0.5201401153216507 >10 0.4025951201025127 5.738605086327688 >50 0.040675770601627914 2.1840232991562956 >100 0.027945742318123832 3.895507662917604 >500 0.0015519571912347425 0.8468808632320255 >1k 6.035389073681831E-4 0.963754578354252 >5k 8.621984390974045E-5 0.4359260936798823 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6676 0.22370532553380496 No Hit NNNNNNNNNNNNNNNNNNNNNNNNN 6539 0.21911460809849467 No Hit TATCAACGCAGAGTACTTTTTTTTT 4697 0.15739123936972466 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 3810 0.12766885714256992 No Hit GGTATCAACGCAGAGTACTTTTTTT 3633 0.12173778425169464 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 3234 0.10836773858243337 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.350888638912596E-5 0.0 0.0 0.0 0.0 2 3.350888638912596E-5 3.350888638912596E-5 0.0 0.0 0.0 3 3.350888638912596E-5 3.350888638912596E-5 0.0 0.0 0.0 4 3.350888638912596E-5 3.350888638912596E-5 0.0 3.350888638912596E-5 0.0 5 6.701777277825192E-5 6.701777277825192E-5 0.0 3.350888638912596E-5 0.0 6 6.701777277825192E-5 6.701777277825192E-5 0.0 3.350888638912596E-5 0.0 7 6.701777277825192E-5 6.701777277825192E-5 0.0 3.350888638912596E-5 0.0 8 6.701777277825192E-5 1.0052665916737789E-4 0.0 3.350888638912596E-5 0.0 9 6.701777277825192E-5 1.0052665916737789E-4 0.0 3.350888638912596E-5 0.0 10 6.701777277825192E-5 1.0052665916737789E-4 0.0 3.350888638912596E-5 0.0 11 6.701777277825192E-5 1.0052665916737789E-4 0.0 6.701777277825192E-5 0.0 12 6.701777277825192E-5 1.0052665916737789E-4 0.0 1.3403554555650385E-4 0.0 13 6.701777277825192E-5 1.0052665916737789E-4 0.0 1.3403554555650385E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3115 0.0 13.544777 1 CGCGGGT 65 5.4463897E-5 13.156256 15 TATAACG 175 0.0 13.032711 2 TAACGCA 150 1.8189894E-12 12.67133 4 ACATGGT 1790 0.0 12.476659 3 ACGGCCC 145 7.2759576E-12 12.450556 15 TACATGT 2380 0.0 12.4178095 2 ATAACGC 155 1.8189894E-12 12.262578 3 GCCCCCG 170 1.8189894E-12 11.737049 18 AACGTAG 100 1.9376403E-6 11.3969145 5 CATGGTA 1225 0.0 11.249039 4 CTCAACG 255 0.0 11.180586 4 ACGCGTC 85 5.3155833E-5 11.178705 14 TTAGACG 60 0.005861714 11.087415 3 GTATCAA 7915 0.0 10.96142 1 ACATGTG 2125 0.0 10.822807 3 CAACGTA 80 3.760046E-4 10.691435 4 CATGTGA 1450 0.0 10.552159 4 GCGGGTA 100 2.395105E-5 10.45209 16 TTAGACT 425 0.0 10.286139 4 >>END_MODULE