FastQCFastQC Report
Thu 26 May 2016
SRR1364276_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364276_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2984283
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT210430.7051274962863776No Hit
TATCAACGCAGAGTACTTTTTTTTT172650.5785309235082599No Hit
GGTATCAACGCAGAGTACTTTTTTT135370.45360979504959814No Hit
GTACTTTTTTTTTTTTTTTTTTTTT105700.35418892913306144No Hit
ACGCAGAGTACTTTTTTTTTTTTTT70050.23472974915582737No Hit
GAGTACTTTTTTTTTTTTTTTTTTT65140.21827688593876654No Hit
GTGGTATCAACGCAGAGTACATGGG39820.1334323856014996No Hit
GGTATCAACGCAGAGTACATGGGAT33660.112790911585798No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTGCG400.005374480514.2121979
GGTATCA51400.013.7935441
CGGTTTG2350.013.40793414
GGACCGA1700.013.3824656
TATAACG2700.012.9911662
ATAACGC2700.012.6385713
TAACGCA2950.011.8892244
CGTCTTA1052.544657E-711.82342215
GTCGGTT2650.011.79821312
TATCACG2050.011.5610252
CGAGTGG1657.2759576E-1211.48441210
GGACCGT752.1176068E-411.3750956
CGAAATC2950.011.24053313
AGGACCG2200.011.2027455
CGTAAGA600.00597053211.0600472
TGCCGCG600.005997401711.05337512
AGACGAA2950.010.91992410
ACGAAAT2950.010.91955812
TTGCGCG4200.010.91392818
TTCAACG2000.010.9020462
Produced by FastQC (version 0.11.5)