##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364276_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2984283 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.969497530897705 31.0 28.0 33.0 25.0 34.0 2 29.038355946805314 31.0 28.0 34.0 25.0 34.0 3 29.10780076822473 31.0 28.0 34.0 25.0 34.0 4 32.56288528936431 35.0 32.0 37.0 26.0 37.0 5 31.828625502340092 35.0 32.0 37.0 20.0 37.0 6 31.84562992182712 35.0 32.0 37.0 21.0 37.0 7 31.403274756449036 35.0 32.0 37.0 17.0 37.0 8 31.221395557995002 35.0 32.0 37.0 16.0 37.0 9 31.539645536298 37.0 30.0 39.0 10.0 39.0 10 31.48155888700904 37.0 30.0 39.0 10.0 39.0 11 32.11742150459591 37.0 31.0 39.0 15.0 39.0 12 31.96885114447926 37.0 31.0 39.0 10.0 39.0 13 32.064810877520664 37.0 32.0 39.0 10.0 39.0 14 32.47651915049612 38.0 31.0 40.0 10.0 41.0 15 32.77635365010624 38.0 31.0 40.0 10.0 41.0 16 32.81062821454936 38.0 32.0 40.0 10.0 41.0 17 32.83253029287102 38.0 32.0 40.0 10.0 41.0 18 32.73258099181612 38.0 32.0 40.0 8.0 41.0 19 32.620725313249444 38.0 31.0 40.0 2.0 41.0 20 32.28435775025358 38.0 31.0 40.0 2.0 41.0 21 31.82632846817812 38.0 30.0 40.0 2.0 41.0 22 31.870043491183644 38.0 30.0 40.0 2.0 41.0 23 31.762006820398735 38.0 30.0 40.0 2.0 41.0 24 31.762122090967914 38.0 31.0 40.0 2.0 41.0 25 31.64993098844848 38.0 31.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9113638693522859 1101 2 0.9550838501740913 1101 3 0.9392694812098483 1101 4 0.9654086669075639 1101 5 1.3105595918029636 1101 6 1.1852185288615615 1101 7 1.422677475716828 1101 8 1.3914772001087563 1101 9 2.317496738853528 1101 10 2.4481216149272953 1101 11 1.536594412518955 1101 12 1.633265728186597 1101 13 1.4213971120355282 1101 14 1.936753430269107 1101 15 1.6407881891473153 1101 16 1.6166144977236598 1101 17 1.5777476504460566 1101 18 1.6681181310101678 1101 19 1.5506495950644918 1101 20 2.264849423888254 1101 21 2.688789024436666 1101 22 2.639893102775769 1101 23 2.4980245495551223 1101 24 2.391238477748555 1101 25 2.473857437741806 1102 1 0.5731276712090612 1102 2 0.808023631300852 1102 3 0.7263187684485679 1102 4 0.6775608224307774 1102 5 1.050427784087475 1102 6 0.8375056192426555 1102 7 1.2037896693003027 1102 8 1.2190386170517193 1102 9 2.302645084341293 1102 10 2.2574161102212145 1102 11 1.5807424466288644 1102 12 1.6000616233444696 1102 13 1.3021104613253058 1102 14 1.847278669916463 1102 15 1.3997638896472537 1102 16 1.2828424958046156 1102 17 1.4706883937704802 1102 18 1.3273463415764297 1102 19 1.3726646775128089 1102 20 1.8508551944014364 1102 21 2.5022512388009375 1102 22 2.3763035308601808 1102 23 2.2597396401553844 1102 24 2.255246739284569 1102 25 2.213368614603091 1103 1 0.408475941448021 1103 2 0.4714315316245994 1103 3 0.5511679829293712 1103 4 0.539718742622405 1103 5 0.765673353082839 1103 6 0.5754050454315802 1103 7 0.883834397740781 1103 8 0.8703757286880993 1103 9 1.7445901384012927 1103 10 1.9309906215815644 1103 11 1.1149089887456292 1103 12 1.2329336612439654 1103 13 1.0738785116111416 1103 14 1.7967400600482932 1103 15 1.1892048028404503 1103 16 1.0098909877223576 1103 17 1.0161863389308579 1103 18 0.7573880314000334 1103 19 0.7925992597644225 1103 20 1.4354028057881187 1103 21 2.0567550099582235 1103 22 1.6730562244437799 1103 23 1.6992921340317402 1103 24 1.5673874562298558 1103 25 1.365305669420671 1104 1 0.5207389170253833 1104 2 0.7120883627185997 1104 3 0.5364814060434853 1104 4 0.467786372069682 1104 5 0.8103444907970214 1104 6 0.6012827601576873 1104 7 0.8384109701470841 1104 8 0.8902683629661503 1104 9 1.7072347427772172 1104 10 1.793258654100086 1104 11 1.2236227539834168 1104 12 0.8835798366768266 1104 13 1.0664496363449913 1104 14 1.5123432799424918 1104 15 0.6665380678669592 1104 16 0.8476878975094806 1104 17 0.38243685237556235 1104 18 0.8957334388792049 1104 19 1.0933350929851855 1104 20 1.630354850230649 1104 21 2.291013260370434 1104 22 2.236126851971047 1104 23 2.0534031425809722 1104 24 1.8226795324452318 1104 25 1.699563686044641 1105 1 -0.16473401015419142 1105 2 0.19775147913927782 1105 3 0.19303619255465776 1105 4 0.5482893762590422 1105 5 0.5795797657040538 1105 6 0.3769819408971671 1105 7 0.20232245629901513 1105 8 0.5521044125361527 1105 9 1.468089927154729 1105 10 1.557813679401292 1105 11 0.7200803389031201 1105 12 0.9813517851684175 1105 13 0.6084246406467244 1105 14 0.7318323043798429 1105 15 0.7526141505866377 1105 16 0.22310038974002566 1105 17 0.6193636246014691 1105 18 0.33897870082836334 1105 19 0.5482215096948693 1105 20 1.227588537886259 1105 21 1.906709772280081 1105 22 1.0682984366589885 1105 23 1.5190346193217685 1105 24 1.2849866304790716 1105 25 0.864626245522679 1106 1 0.14575217697955978 1106 2 0.22083230998622128 1106 3 -0.21382301709610374 1106 4 0.056546758504445904 1106 5 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1107 17 0.6564437312227369 1107 18 0.7876537981256746 1107 19 0.9116613440639938 1107 20 1.3160774086602736 1107 21 1.9538483474650334 1107 22 1.6501688896550952 1107 23 1.5913925508114666 1107 24 1.1754822967357548 1107 25 1.365540966543584 1108 1 -0.012094029961506436 1108 2 0.13038767263927298 1108 3 0.23080371875201777 1108 4 -0.7680954973600898 1108 5 0.22992879682047018 1108 6 0.2976772359315021 1108 7 0.45962771470438213 1108 8 0.6718321347676799 1108 9 1.452553636802783 1108 10 1.264471049251938 1108 11 0.6397048009378885 1108 12 0.8391968657448636 1108 13 0.5291585308180728 1108 14 0.9353241447853122 1108 15 0.7826666545371808 1108 16 0.7171889255279069 1108 17 0.4873150966782518 1108 18 0.3712735822393398 1108 19 0.6025543599821432 1108 20 1.3189652191414005 1108 21 1.670968053849812 1108 22 1.5700469420076892 1108 23 1.0914662585955917 1108 24 1.1218063183682112 1108 25 0.8361794449138955 1109 1 0.2865407463363887 1109 2 0.4251997311345761 1109 3 0.008141707294999634 1109 4 0.5508485503096949 1109 5 0.6779897266992876 1109 6 0.49833780711298203 1109 7 0.5501419384945301 1109 8 0.7608605858874604 1109 9 1.8162768183811835 1109 10 2.0397494260794033 1109 11 0.9845256565288061 1109 12 0.8557313498435057 1109 13 0.7856460017039595 1109 14 1.0634201804585999 1109 15 0.8363792279833433 1109 16 0.7235087501700121 1109 17 0.1841677716516017 1109 18 0.7469585279384745 1109 19 0.9555945639626842 1109 20 1.3777312189051436 1109 21 1.6316171629982463 1109 22 1.5963160428766763 1109 23 1.2389437043319695 1109 24 1.1539373229758816 1109 25 1.406437047175153 1110 1 0.11966380524952669 1110 2 0.1006463849048842 1110 3 0.1062404074083716 1110 4 0.18062448799686592 1110 5 0.24237294813607946 1110 6 0.03595327245560398 1110 7 0.06461947440226012 1110 8 0.34652166060428513 1110 9 1.126403669977094 1110 10 0.8980603058697056 1110 11 -0.05203410335485614 1110 12 0.25380664982376544 1110 13 -0.058426671408891906 1110 14 0.7291790441470027 1110 15 0.04983429741901091 1110 16 0.1842093845381001 1110 17 -0.08273152530014016 1110 18 0.2497682843589999 1110 19 0.16171265450329741 1110 20 0.9212093624064899 1110 21 1.0110097228214983 1110 22 1.2029892152634112 1110 23 0.9658019920886751 1110 24 0.6630929980089881 1110 25 1.0442552710482147 1111 1 -0.18466164393553797 1111 2 -0.1023076389330484 1111 3 -0.36351309268647825 1111 4 -0.1717856965701472 1111 5 -0.07017898982491388 1111 6 -0.4582035687647199 1111 7 0.015988489049004073 1111 8 0.019122439986880124 1111 9 0.9338540816594545 1111 10 0.9368786879128734 1111 11 -0.0524289739739352 1111 12 0.04767471229648734 1111 13 -0.10184580672750698 1111 14 0.04226123092385592 1111 15 0.01980718177676266 1111 16 0.009755945058152804 1111 17 -0.7419952363428521 1111 18 -0.34264291501813204 1111 19 -0.009352013955925997 1111 20 0.4235163711020711 1111 21 1.1264677443165212 1111 22 1.1071332408214722 1111 23 0.8404137798451394 1111 24 0.7456126410063639 1111 25 0.8305186791216883 1112 1 0.1025643760548327 1112 2 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1.6996158797054584 1203 13 1.6370744136111597 1203 14 1.9104638862959646 1203 15 1.669971089978013 1203 16 1.5130425732298818 1203 17 1.639442170445733 1203 18 1.7057601544200338 1203 19 1.8200148086203285 1203 20 2.5211858529439333 1203 21 2.9483351212943845 1203 22 2.7896331519479283 1203 23 2.726557675417883 1203 24 2.5119463281410326 1203 25 2.3529014803141166 1204 1 0.5958004521134761 1204 2 0.66412044094632 1204 3 0.5668225764251495 1204 4 0.5867920380217839 1204 5 0.8201541719938277 1204 6 0.830173032255054 1204 7 1.0898574600613493 1204 8 0.9912341532516287 1204 9 1.7350652926636272 1204 10 2.054934123286273 1204 11 1.0640783276372154 1204 12 0.9016830111264049 1204 13 0.9029500592502302 1204 14 1.1147001867137547 1204 15 1.0853710616760281 1204 16 1.0443569386609042 1204 17 1.220149143486978 1204 18 1.1588880532148025 1204 19 1.342818869732561 1204 20 1.1922089641902396 1204 21 2.1578903009757866 1204 22 2.0415291204190673 1204 23 1.9826939277557223 1204 24 1.8367779229635524 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2203 23 2.7231561738455063 2203 24 2.401407361040821 2203 25 2.578656412730602 2204 1 0.9618031707559105 2204 2 1.1069018811316411 2204 3 0.9924514763856322 2204 4 0.97030651169122 2204 5 1.2698990586306067 2204 6 1.1460225732568716 2204 7 1.3985626771917872 2204 8 1.2995778160715155 2204 9 2.4391531089852165 2204 10 2.583365099232921 2204 11 1.6047824314290402 2204 12 1.8955763594896275 2204 13 1.3876159186305372 2204 14 2.261954526301288 2204 15 1.9756705107284844 2204 16 1.8660781109711735 2204 17 1.7798973134566722 2204 18 1.8956362087463887 2204 19 1.9166549576253544 2204 20 2.598366670584344 2204 21 2.7547500256570885 2204 22 2.5971618946054384 2204 23 2.6578406884590358 2204 24 2.3345326633422907 2204 25 2.570483558535315 2205 1 0.5279889674155775 2205 2 0.6073539843784665 2205 3 0.6702953692581239 2205 4 0.618840576743132 2205 5 0.9136432923888833 2205 6 0.47219935449752626 2205 7 0.49514919206944086 2205 8 0.9923770555840115 2205 9 1.5736842889990612 2205 10 1.6841295966272085 2205 11 1.1059473779825275 2205 12 1.33884661435199 2205 13 1.2512774954501964 2205 14 1.3926506437700112 2205 15 0.3185753070717823 2205 16 0.2774084233939149 2205 17 0.27219518076935856 2205 18 0.7717255068877051 2205 19 0.7670448199062463 2205 20 1.4242165843945465 2205 21 1.5804890664508235 2205 22 1.0373555532408112 2205 23 0.4245403679021429 2205 24 0.49536385222077683 2205 25 0.6577693156030513 2206 1 -1.239400966386146 2206 2 -3.274678285676295 2206 3 -1.0392626313359763 2206 4 -2.045376932195392 2206 5 -0.41179872733327727 2206 6 -0.7971745067358249 2206 7 -0.6752832755959766 2206 8 -0.017725517780959876 2206 9 1.2426906453265758 2206 10 0.6909506450316414 2206 11 0.38917315081922865 2206 12 0.2057896228645717 2206 13 0.15810228708001972 2206 14 0.6646850716629586 2206 15 -0.18927458894314242 2206 16 0.3669776939370948 2206 17 0.5012764030428372 2206 18 -0.1615546110372037 2206 19 0.3201601992371934 2206 20 0.9833632311280276 2206 21 1.4568674480693034 2206 22 1.1517388841871359 2206 23 0.8844873708784924 2206 24 0.701588494430819 2206 25 0.6798482684607414 2207 1 0.4342193010621429 2207 2 0.4929326433973813 2207 3 0.39220166949100843 2207 4 0.48106766188333694 2207 5 0.7394435030663757 2207 6 0.5029579134803015 2207 7 0.5342624528068569 2207 8 0.6733003397212336 2207 9 1.1137530092238705 2207 10 1.5533286871217058 2207 11 0.43294240208379264 2207 12 0.6011176051579348 2207 13 0.7283400071692512 2207 14 1.6695325813559094 2207 15 0.9953821309209943 2207 16 0.25585861257394527 2207 17 0.34719167347798674 2207 18 0.8041219306637188 2207 19 1.0348097397527596 2207 20 1.4666182657375906 2207 21 1.6727592856033553 2207 22 1.6949022102438924 2207 23 0.8649162009269951 2207 24 1.3996043590014473 2207 25 1.0528949910504863 2208 1 0.23845607878332586 2208 2 0.4882763579777709 2208 3 0.47255089485578594 2208 4 0.4015437759736713 2208 5 0.4324973484420056 2208 6 0.5749803837188807 2208 7 0.9415216070537689 2208 8 1.0104244092434378 2208 9 1.8480829644412609 2208 10 1.869341634867368 2208 11 1.1457867799275654 2208 12 1.0643228401738476 2208 13 0.9415985061188721 2208 14 1.6881908738337685 2208 15 1.3750169384159534 2208 16 1.1140358971777786 2208 17 1.024138056694433 2208 18 0.8069386600810518 2208 19 1.060394768073273 2208 20 1.7881086491524805 2208 21 2.1914012005967223 2208 22 1.8848676710691983 2208 23 1.8817845481511704 2208 24 1.5701422968587728 2208 25 1.4792489727798142 2209 1 0.3246607728131252 2209 2 0.21282519807937206 2209 3 0.2804352371297014 2209 4 0.2932775193665762 2209 5 0.5433464770488641 2209 6 0.18521852886156154 2209 7 0.5654825758989759 2209 8 0.5218961436424507 2209 9 1.352833715174114 2209 10 1.2989412870584403 2209 11 0.38632118847523955 2209 12 0.7412803001355961 2209 13 0.40664301367487354 2209 14 0.8686295687026231 2209 15 0.6169266226627954 2209 16 0.3734450988165605 2209 17 -0.012234134617692405 2209 18 0.09507623665679432 2209 19 0.02970241837961396 2209 20 0.8759605349993613 2209 21 1.473852776713528 2209 22 1.4040096783677534 2209 23 1.208407063216324 2209 24 0.7602730861274232 2209 25 0.6731288402918949 2210 1 0.06281343340286227 2210 2 0.2743407442004262 2210 3 0.46361981845374345 2210 4 0.5173640472508936 2210 5 0.27692142090761607 2210 6 0.45205862392013785 2210 7 0.5490974578388368 2210 8 0.5673417158669523 2210 9 1.610603802641485 2210 10 1.6173627517454463 2210 11 0.8814358593279756 2210 12 0.8789689654505075 2210 13 0.7827758974818124 2210 14 1.356639621603243 2210 15 0.8958878757869826 2210 16 0.8774309787724022 2210 17 0.697760295853378 2210 18 0.6374464775339703 2210 19 0.8093068430829504 2210 20 1.421142099660404 2210 21 1.8021182351293632 2210 22 1.7879381188401915 2210 23 1.8356202572519713 2210 24 1.6722850338570971 2210 25 1.591340407073723 2211 1 0.34708882610778247 2211 2 0.3122225427799279 2211 3 0.13127639640017463 2211 4 0.24519362518942955 2211 5 0.38511046637211166 2211 6 0.14601167289188766 2211 7 0.5120248988487148 2211 8 0.5147681454740969 2211 9 1.505454966020416 2211 10 1.444726614159194 2211 11 0.7590016337700014 2211 12 0.13565096442557234 2211 13 -0.021466684722337703 2211 14 0.6310149883683991 2211 15 0.46996307317080976 2211 16 0.45273575462960025 2211 17 0.15452663654787813 2211 18 0.06639955292160948 2211 19 0.0808956588400278 2211 20 0.7867777701744636 2211 21 1.5507945540995607 2211 22 1.4325393258705752 2211 23 1.3939642335828104 2211 24 1.2714536701387011 2211 25 1.0617304042018887 2212 1 0.43474377556926314 2212 2 0.4030432993714115 2212 3 0.27508599360715635 2212 4 0.480315707006973 2212 5 0.35932782665349805 2212 6 0.3658071421366884 2212 7 0.7399715752457858 2212 8 0.5157131552246028 2212 9 -9.045334170247035 2212 10 -1.628056647165991 2212 11 -0.4689306077936166 2212 12 0.40523171989162776 2212 13 0.3906414842574861 2212 14 1.068288105028941 2212 15 0.7735320595291881 2212 16 0.6642739049682191 2212 17 0.7218628453140141 2212 18 0.9428800888768407 2212 19 1.1643729298316572 2212 20 1.659267581471351 2212 21 2.031593062523406 2212 22 1.8476718895441842 2212 23 1.6611485969883013 2212 24 1.4224476202658245 2212 25 1.4333538983088587 2213 1 0.5581357778728311 2213 2 0.6133058700081335 2213 3 0.36740143830355265 2213 4 0.7157375477009253 2213 5 0.6473132853872556 2213 6 0.42166864017506356 2213 7 0.6905078744215416 2213 8 0.9787571029624296 2213 9 1.5403261256173408 2213 10 -8.885702509743826 2213 11 -2.6234288621218056 2213 12 -0.6932673622809205 2213 13 0.3592841794307944 2213 14 0.8034453954583398 2213 15 0.5422251452290823 2213 16 0.9719170680535569 2213 17 0.6102208340121393 2213 18 0.5802563621374688 2213 19 1.0487572655765334 2213 20 1.5740176084662743 2213 21 1.5622862921962266 2213 22 1.8007664077747556 2213 23 1.4794655534004981 2213 24 1.3984131387483494 2213 25 1.1414955676750154 2214 1 -2.0479078034936435 2214 2 -2.2910693144646075 2214 3 -2.192985521891046 2214 4 -2.1356621551071306 2214 5 -1.9625683467257602 2214 6 -2.114789279684892 2214 7 -10.469814456703208 2214 8 -3.776919059597496 2214 9 -3.040248513324979 2214 10 -2.5577763904395816 2214 11 -2.978182660987997 2214 12 -11.816491080640667 2214 13 -5.376916241930388 2214 14 -13.181780755397103 2214 15 -6.917652924651737 2214 16 -4.860771501252216 2214 17 -3.9233715244909106 2214 18 -3.3982051097768746 2214 19 -3.7284673484301614 2214 20 -3.5890339014112875 2214 21 -12.953922373188036 2214 22 -15.015320378379357 2214 23 -9.337032670572306 2214 24 -7.902598026305135 2214 25 -15.602649649264924 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 126096.0 3 1.0 4 2074.0 5 19019.0 6 23594.0 7 19194.0 8 15847.0 9 12092.0 10 10507.0 11 9487.0 12 8913.0 13 8676.0 14 8320.0 15 8300.0 16 8796.0 17 9441.0 18 10900.0 19 13368.0 20 17236.0 21 22873.0 22 28767.0 23 34176.0 24 39470.0 25 45075.0 26 51344.0 27 56724.0 28 63056.0 29 72840.0 30 85412.0 31 103043.0 32 126701.0 33 153630.0 34 185141.0 35 221734.0 36 272876.0 37 366317.0 38 723243.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.98665799657107 20.01980938079764 13.27781567220335 23.715716950427947 2 15.732441619522259 22.265163541747306 39.01640025510093 22.985994583629505 3 17.926138648366084 28.599743988419164 30.263785754496958 23.210331608717798 4 11.903261185350049 18.059312739441935 37.61255216077027 32.42487391443774 5 12.236620892589478 40.0485699608163 33.99260559710658 13.722203549487643 6 28.985935098318265 38.37607401824989 19.06349931322479 13.574491570207059 7 26.955629811919625 33.6719436938386 22.34895397713869 17.02347251710309 8 26.61996180338701 34.606117961627056 20.550475077497822 18.223445157488115 9 27.131818274733916 15.490104154274729 20.449705458641056 36.928372112350296 10 14.716542467527157 31.282500487570147 31.993314089671355 22.007642955231344 11 33.50824658042871 24.116112281369613 23.780605908692134 18.59503522950955 12 22.9854955613395 28.01106255409885 29.94379249366899 19.05964939089266 13 28.11050611664706 22.761560508177478 27.021549590799378 22.106383784376085 14 22.111741757862475 21.801712866064975 28.98099950473119 27.105545871341363 15 23.70193765654975 29.536219014668553 24.743832254065058 22.01801107471664 16 22.15284356259008 29.1008143708321 26.520917866933484 22.225424199644333 17 21.20486279939696 28.798840026848005 27.984620369833596 22.011676803921443 18 20.77683683973493 27.29300033241116 30.5053252804049 21.424837547449012 19 22.235659650385596 26.9976362189959 30.23397879902232 20.532725331596186 20 22.776054661801716 26.276374143714175 29.992833608686663 20.954737585797442 21 23.636865756480518 25.57937897491447 29.340432320545684 21.44332294805933 22 23.21625213721328 26.30704290110073 29.017005883996987 21.459699077689002 23 22.062228930561318 26.285332441469777 29.675128870038815 21.97730975793009 24 21.955406593642266 26.652096636695568 29.619182040930127 21.773314728732046 25 21.821743533242056 26.361358352584325 30.259239972897824 21.557658141275798 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1464.0 1 1464.0 2 2223.5 3 2983.0 4 2983.0 5 2983.0 6 8337.0 7 13691.0 8 13691.0 9 13691.0 10 13806.5 11 13922.0 12 13922.0 13 13922.0 14 15311.0 15 16700.0 16 16700.0 17 16700.0 18 30212.0 19 43724.0 20 43724.0 21 43724.0 22 66284.5 23 88845.0 24 88845.0 25 88845.0 26 129788.5 27 170732.0 28 170732.0 29 170732.0 30 201398.0 31 232064.0 32 232064.0 33 232064.0 34 257986.0 35 283908.0 36 283908.0 37 283908.0 38 302677.0 39 321446.0 40 321446.0 41 321446.0 42 339103.5 43 356761.0 44 356761.0 45 356761.0 46 369164.0 47 381567.0 48 381567.0 49 381567.0 50 364308.0 51 347049.0 52 347049.0 53 347049.0 54 309183.5 55 271318.0 56 271318.0 57 271318.0 58 231543.0 59 191768.0 60 191768.0 61 191768.0 62 156784.0 63 121800.0 64 121800.0 65 121800.0 66 93197.5 67 64595.0 68 64595.0 69 64595.0 70 48647.0 71 32699.0 72 32699.0 73 32699.0 74 23541.0 75 14383.0 76 14383.0 77 14383.0 78 10997.0 79 7611.0 80 7611.0 81 7611.0 82 5345.0 83 3079.0 84 3079.0 85 3079.0 86 2212.5 87 1346.0 88 1346.0 89 1346.0 90 1062.0 91 778.0 92 778.0 93 778.0 94 413.5 95 49.0 96 49.0 97 49.0 98 25.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.028415535657978816 2 0.012264252418420102 3 0.0014408821147324166 4 0.0 5 0.004490190776142879 6 0.0015414087738997943 7 9.047399325064011E-4 8 0.04342751676030725 9 0.005093350731147146 10 0.0032503619797452183 11 0.004590717435310258 12 0.005428439595038406 13 0.0016754443194562981 14 0.0015078998875106683 15 2.680710911130077E-4 16 5.361421822260154E-4 17 0.002546675365573573 18 9.047399325064011E-4 19 0.002714219797519203 20 0.7787130107968983 21 0.019133574128190924 22 0.008611783802005373 23 0.009013890438674885 24 0.002513166479184447 25 7.036866141716453E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2984283.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.636700460612495 #Duplication Level Percentage of deduplicated Percentage of total 1 71.29253915343213 30.396786369613743 2 14.031803822145267 11.965396329737708 3 5.583775106123863 7.142212399176836 4 2.8104956272932555 4.793210408270551 5 1.5799835018144568 3.3682641649786302 6 0.9977783095340624 2.552518494582007 7 0.6772035780627732 2.0211608276102235 8 0.4826763462576925 1.6463781435849694 9 0.3517468525995694 1.349759267302595 >10 1.906394999717615 14.848988392810526 >50 0.16582587695310708 4.938112602940334 >100 0.11046442824324068 8.739207038819757 >500 0.006428026815869357 1.875305406735515 >1k 0.0023899074058909542 1.6945949889598597 >5k 1.648212004062727E-4 0.4750192762658279 >10k+ 3.296424008125454E-4 2.193085888610966 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 21043 0.7051274962863776 No Hit TATCAACGCAGAGTACTTTTTTTTT 17265 0.5785309235082599 No Hit GGTATCAACGCAGAGTACTTTTTTT 13537 0.45360979504959814 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 10570 0.35418892913306144 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 7005 0.23472974915582737 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 6514 0.21827688593876654 No Hit GTGGTATCAACGCAGAGTACATGGG 3982 0.1334323856014996 No Hit GGTATCAACGCAGAGTACATGGGAT 3366 0.112790911585798 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.0105331833475577E-4 0.0 2 3.350888638912596E-5 0.0 0.0 2.0105331833475577E-4 0.0 3 3.350888638912596E-5 0.0 0.0 2.0105331833475577E-4 0.0 4 3.350888638912596E-5 0.0 0.0 2.0105331833475577E-4 0.0 5 6.701777277825192E-5 0.0 0.0 2.3456220472388176E-4 0.0 6 6.701777277825192E-5 0.0 0.0 3.0157997750213366E-4 0.0 7 1.0052665916737789E-4 0.0 0.0 3.0157997750213366E-4 0.0 8 1.675444319456298E-4 0.0 0.0 3.0157997750213366E-4 0.0 9 1.675444319456298E-4 0.0 0.0 3.0157997750213366E-4 0.0 10 1.675444319456298E-4 0.0 0.0 3.0157997750213366E-4 0.0 11 1.675444319456298E-4 0.0 0.0 3.0157997750213366E-4 0.0 12 2.3456220472388176E-4 0.0 0.0 3.685977502803856E-4 0.0 13 2.3456220472388176E-4 0.0 0.0 5.026332958368894E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTTGCG 40 0.0053744805 14.212197 9 GGTATCA 5140 0.0 13.793544 1 CGGTTTG 235 0.0 13.407934 14 GGACCGA 170 0.0 13.382465 6 TATAACG 270 0.0 12.991166 2 ATAACGC 270 0.0 12.638571 3 TAACGCA 295 0.0 11.889224 4 CGTCTTA 105 2.544657E-7 11.823422 15 GTCGGTT 265 0.0 11.798213 12 TATCACG 205 0.0 11.561025 2 CGAGTGG 165 7.2759576E-12 11.484412 10 GGACCGT 75 2.1176068E-4 11.375095 6 CGAAATC 295 0.0 11.240533 13 AGGACCG 220 0.0 11.202745 5 CGTAAGA 60 0.005970532 11.060047 2 TGCCGCG 60 0.0059974017 11.053375 12 AGACGAA 295 0.0 10.919924 10 ACGAAAT 295 0.0 10.919558 12 TTGCGCG 420 0.0 10.913928 18 TTCAACG 200 0.0 10.902046 2 >>END_MODULE