FastQCFastQC Report
Thu 26 May 2016
SRR1364275_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364275_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2905923
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN56870.19570374025739842No Hit
GTATCAACGCAGAGTACTTTTTTTT48420.16662519963536543No Hit
TATCAACGCAGAGTACTTTTTTTTT34810.1197898223731324No Hit
GTACTTTTTTTTTTTTTTTTTTTTT34430.11848214835699362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA2450.013.962384
ATAACGC2400.013.4614143
TATAACG2400.013.4607182
AAGACCG655.4660377E-513.1511825
GGTATCA25350.013.043731
ATCACGC1600.012.4716043
TCACGCA1701.8189894E-1211.7379814
TTGCGCG4700.011.32037318
TATCACG1850.011.2992982
TGCGCGC4900.011.24611419
TTTGCGC4750.011.20140517
ATTTGCG4900.010.85906716
ACATGGT13450.010.7385183
GTATCAA63850.010.7293861
TACATGT16650.010.7286272
GCCCCCG1701.382432E-1010.61879418
CATGGTA9100.010.1286934
CGTGCGC856.605759E-410.05730710
AAAGGCG1451.6233571E-79.8255965
CAACTCA14450.09.7938645
Produced by FastQC (version 0.11.5)