FastQCFastQC Report
Thu 26 May 2016
SRR1364275_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364275_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2905923
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT144280.49650317644342257No Hit
TATCAACGCAGAGTACTTTTTTTTT119870.41250232714356166No Hit
GGTATCAACGCAGAGTACTTTTTTT94950.32674644166414596No Hit
GTACTTTTTTTTTTTTTTTTTTTTT90180.3103316915141936No Hit
GAGTACTTTTTTTTTTTTTTTTTTT49710.17106440879541543No Hit
ACGCAGAGTACTTTTTTTTTTTTTT47220.16249570274229566No Hit
GTGGTATCAACGCAGAGTACATGGG31000.10667866973763586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG2650.014.3127772
TCGTGCG400.00536496314.2159789
CGGTTTG1700.012.91202414
TATCACG2350.012.9119522
GGTATCA40300.012.6808581
ATTAACG604.1657983E-412.6405583
ATAACGC3300.012.3532723
TAACGCA3050.011.8122794
ACCGAGT1552.7284841E-1111.6217588
ATCACGC2700.011.5871783
CGGTGTT2150.011.09729114
ACGGGTT951.2914979E-511.05228915
CCGCGAA951.2937333E-511.05056514
GCGCCAC1553.274181E-1011.00516113
GTCGGTT2250.010.95081512
TCGACCG803.6121692E-410.7379318
GGTCGGT2150.010.57860411
AAGACCG1355.864058E-810.5343435
GCGCCGA1951.4551915E-1110.27904519
CCGTCTA750.002701794310.109149
Produced by FastQC (version 0.11.5)