FastQCFastQC Report
Thu 26 May 2016
SRR1364274_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364274_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2901870
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT149560.5153917990812821No Hit
TATCAACGCAGAGTACTTTTTTTTT107140.3692101989406831No Hit
GGTATCAACGCAGAGTACTTTTTTT82820.2854021717030742No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA78200.2694814033709298No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN59420.20476451391688807No Hit
GTACTTTTTTTTTTTTTTTTTTTTT57910.19956097275205298No Hit
ACGCAGAGTACTTTTTTTTTTTTTT40750.14042669037551647No Hit
GAGTACTTTTTTTTTTTTTTTTTTT37430.12898579192038237No Hit
GAACAAAAAAAAAAAAAAAAAAAAA30360.1046221918969492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44400.014.5963011
CCGTATA400.00529044714.2459819
CGCGGTA551.9669213E-413.8133313
ACATGGT17300.012.9087943
AACTGGC4200.012.89613117
GATAGTC2700.012.3115557
CTCAACG4450.012.1724724
GGACCGA802.8751247E-511.8722676
CATGGTA14100.011.794594
CGTCACT2500.011.78319551
TACATGT20700.011.7060732
CGACCGT2600.011.69527719
TCAATAC1552.5465852E-1111.6489243
GTGGTAT10250.011.5885091
GGCGAGT1406.7848305E-1011.538444516
TCGACCG2800.011.538444518
GTATCAA117600.011.47421
TTGCGCG11000.011.40269818
TAGATAG3700.011.2945015
TGCGCGC11200.011.28411519
Produced by FastQC (version 0.11.5)