FastQCFastQC Report
Thu 26 May 2016
SRR1364274_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364274_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2901870
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT491981.6953895246858062No Hit
TATCAACGCAGAGTACTTTTTTTTT403291.3897590174611543No Hit
GGTATCAACGCAGAGTACTTTTTTT321741.108733334022544No Hit
ACGCAGAGTACTTTTTTTTTTTTTT172010.5927557058034991No Hit
GTACTTTTTTTTTTTTTTTTTTTTT152160.5243515388353027No Hit
GAGTACTTTTTTTTTTTTTTTTTTT116510.40149972259267297No Hit
ATCAACGCAGAGTACTTTTTTTTTT67140.2313680488788264No Hit
GCAGAGTACTTTTTTTTTTTTTTTT66160.22799091620231093No Hit
GTGGTATCAACGCAGAGTACATGGG62540.21551620162171292No Hit
GTGGTATCAACGCAGAGTACTTTTT43560.1501101014173619No Hit
GGTATCAACGCAGAGTACATGGGAT43210.14890398260432067No Hit
GGTCGGGAGTGGGTAATTTGCGCGC40930.14104698005079483No Hit
GGTATCAACGCAGAGTACATGGGAG32420.11172106262513483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86500.014.561481
CGAGTAT1300.014.00432318
GTGGTAT20200.013.960741
TGGTATC20800.013.2875652
ACGGCCC3000.013.0952715
CGCGCCA1800.012.61384810
GCCCCCG3250.012.38277118
TCGCGGT959.382529E-712.10345917
CCTCTAG4250.011.8013171
TCGACCG2200.011.75961118
TATCACG1451.03682396E-1011.7497982
CGACCGT2050.011.68504519
ATCACGC1552.7284841E-1111.6013983
GCCTACG907.7787245E-611.5684752
ACGGTGA3450.011.5169910
ATCGACG1105.2154064E-711.1860452
GTTCGAC2400.011.17888916
TCACGCA1701.2732926E-1111.1346624
CGGTGAT3850.011.05740211
TGGACCG600.006043977611.0418735
Produced by FastQC (version 0.11.5)