FastQCFastQC Report
Thu 26 May 2016
SRR1364273_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364273_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3162967
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN66410.20996109033069266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA2050.012.5158634
ATAACGC1800.011.6145143
TACATGT14850.011.5168822
GGTATCA17150.011.3014531
GTATCAA40600.010.6710471
TATAACG1909.094947E-1210.5015772
TGACCGA4900.010.47129319
GTATAAC4100.010.42791
TTGACCG5000.010.26186818
AGGACCG1803.965397E-1010.025155
ACATGGT13250.09.9689593
GGACCGA1451.6242302E-79.8253226
GTCTTAG3700.09.7577581
CCAATAC3150.09.6536233
ACATGTG14000.09.6385383
TTGCGCG4650.09.60389818
TTACGCT900.00111120019.5027854
GTCCTAC2201.6370905E-119.5009761
GCTATAA1901.0513759E-99.5009761
TGGACCG1106.860324E-59.4975115
Produced by FastQC (version 0.11.5)