FastQCFastQC Report
Thu 26 May 2016
SRR1364273_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364273_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3162967
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47920.15150331950981466No Hit
TATCAACGCAGAGTACTTTTTTTTT41540.13133238506756473No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG1500.013.9032682
TAACGCA1750.013.5410724
TATAACG1850.012.297732
ATAACGC2100.011.7354113
GGTATCA26150.011.7072381
CGATTCG907.694889E-611.5798299
TTAACGA803.869353E-410.65836812
AACTGGC6100.010.64629117
TACGATT909.7398864E-510.5319457
TGTTCGG1002.4734883E-510.4216810
TGCGCCG750.00270916110.10587210
CGTTCGC1252.4040673E-69.932363515
GGTCGGT2400.09.86885911
AAACGCA1451.6724698E-79.8056045
GTAATAC1451.67287E-79.8054493
ATCACGC2151.0913936E-119.6990343
GTATCAA51250.09.6538441
CGAGTAT800.00432483439.55034918
TTGACCG3600.09.55034918
TACTGGA8300.09.4795012
Produced by FastQC (version 0.11.5)