FastQCFastQC Report
Thu 26 May 2016
SRR1364272_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364272_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2453070
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN50110.20427464360984396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGGT1450.013.76087117
CGCGCCA957.4112904E-812.99760910
CGTAAGA550.003065085512.0931342
GGTATCA17500.011.8907591
AAGACCG802.874544E-511.872335
ACATGGT15800.011.7877023
TTGCGCG4200.011.76310618
GTATCAA43800.011.4107741
CGCGGTT2050.011.12308918
TTCGCGG1900.011.00179916
GCTTATA3200.010.9863961
GACCCGG1752.0008883E-1110.8549246
TGGACCG1408.38736E-910.8547035
TAGACAG3550.010.7018195
CGCTATT803.7787613E-410.68553411
TACATGT12550.010.675272
TTTGCGC4550.010.65009317
ATTTGCG4600.010.53433116
CCTACAC2550.010.4339323
CATGGTA11650.010.3587654
Produced by FastQC (version 0.11.5)