FastQCFastQC Report
Thu 26 May 2016
SRR1364272_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364272_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2453070
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT59030.24063724231269387No Hit
TATCAACGCAGAGTACTTTTTTTTT50850.20729127175335396No Hit
GGTATCAACGCAGAGTACTTTTTTT40170.16375398989837225No Hit
GTGGTATCAACGCAGAGTACATGGG31460.12824746134435625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25350.013.2011551
TTTATAC1600.012.4439643
TACGATA701.11122055E-412.1907522
GCTTATA3500.012.1887641
TTATACT2550.011.8978714
ACCGAGT1407.039489E-1011.5127838
GTGGTAT8400.011.5116091
GGACCGA1657.2759576E-1211.4922616
TCGCGGT1001.8242445E-611.45395617
ACCAGTT2900.011.43782812
TAGCCTA600.00596407711.0615275
GACCGAT600.005964982311.0613017
CGATACT1553.237801E-1011.0103274
CTTCGCG1053.2774715E-610.90852815
CCGTCCA2200.010.7693829
TTCGCGC1002.278042E-510.49945917
TCGCGCA1002.2795914E-510.49881318
TGGTATC8000.010.4298662
GTATTGG2550.010.4095751
ACCGTCC1553.787136E-910.3986438
Produced by FastQC (version 0.11.5)