FastQCFastQC Report
Thu 26 May 2016
SRR1364271_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364271_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1156788
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN22720.19640591015812747No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG953.0559022E-1014.9975375
CGGCGGA456.751111E-414.78047618
CGACCTA456.751111E-414.78047618
GGACCGA851.718763E-814.5270256
TACGGTG400.005283766414.247665
ACATGGT6100.013.085493
AACTATC801.9940362E-613.06488519
GGTATCA8300.012.819951
CGCGGCG604.0843102E-412.6689816
ACACCTA1301.9826984E-1012.4210376
TTGCGCG4000.012.11471118
CCCTAGA1052.7154783E-711.7625221
CAACGGA658.002573E-411.69545654
GTCCTAT907.4514337E-611.611721
ATTTGCG4200.011.53781916
TTTGCGC4200.011.53681917
AATTTGC4150.011.44787215
TAGGTTG752.0760967E-411.3981285
TGCGCGC4450.011.31667119
TTACACC2650.011.11624
Produced by FastQC (version 0.11.5)