##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364271_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1156788 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.753264210901218 30.0 23.0 31.0 7.0 33.0 2 25.414802885230483 30.0 25.0 31.0 7.0 34.0 3 24.975919528902445 30.0 23.0 31.0 7.0 34.0 4 28.034027842612474 33.0 26.0 35.0 7.0 37.0 5 28.351647838670527 33.0 27.0 35.0 7.0 37.0 6 29.179104555026505 35.0 28.0 35.0 2.0 37.0 7 29.146535060875458 35.0 29.0 35.0 2.0 37.0 8 27.460027247862183 33.0 23.0 35.0 2.0 37.0 9 27.294053880226972 33.0 17.0 37.0 2.0 39.0 10 28.991036386961138 34.0 27.0 37.0 2.0 39.0 11 29.50025588093929 35.0 27.0 38.0 2.0 39.0 12 28.078307347586595 34.0 22.0 38.0 2.0 39.0 13 27.878664889331493 33.0 21.0 37.0 2.0 39.0 14 28.385837335795323 34.0 21.0 39.0 2.0 40.0 15 27.888256102241723 33.0 17.0 38.0 2.0 40.0 16 28.681977164355093 34.0 25.0 39.0 2.0 40.0 17 29.241079610092775 34.0 27.0 39.0 2.0 40.0 18 29.783730467466814 36.0 27.0 39.0 2.0 41.0 19 30.414330024170376 36.0 28.0 40.0 2.0 41.0 20 30.439698544590712 36.0 29.0 40.0 2.0 41.0 21 30.59312250818646 36.0 30.0 40.0 2.0 41.0 22 30.480422514756377 36.0 29.0 40.0 2.0 41.0 23 30.124839642181627 36.0 28.0 40.0 2.0 41.0 24 30.05958827373728 36.0 29.0 40.0 2.0 41.0 25 29.74518840098618 36.0 28.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -1.2417249558291168 1101 2 -1.3348580810644002 1101 3 -3.570316979522218 1101 4 -4.268666208988751 1101 5 -4.737188077393796 1101 6 -2.8689329597016524 1101 7 -2.2698665604657435 1101 8 -6.121565047973416 1101 9 -7.168466299876872 1101 10 -7.610657737684786 1101 11 -4.483583697679556 1101 12 -1.8238973711678028 1101 13 -1.1730384345627591 1101 14 -8.029656682903653 1101 15 -10.240475106914843 1101 16 -5.255251171980003 1101 17 -3.369615772044412 1101 18 -8.317928389816274 1101 19 -4.619960522864183 1101 20 -3.4563075483387635 1101 21 -3.675375703375842 1101 22 -3.468869925746283 1101 23 -8.451731769529903 1101 24 -5.3208380245620255 1101 25 -4.2083447278931665 1102 1 1.3771846279002844 1102 2 0.8537992251942939 1102 3 1.4432747935728152 1102 4 1.3645035883458227 1102 5 0.9391108103014787 1102 6 0.16192107108649623 1102 7 -0.11521927431628853 1102 8 1.2740150432105644 1102 9 -3.7331938840392027 1102 10 -1.277590391238995 1102 11 -0.6713117304566687 1102 12 -4.535879509039091 1102 13 -7.385680049611835 1102 14 -7.118633442327365 1102 15 -1.9492945329657836 1102 16 -0.3520916869593016 1102 17 -4.691230220577626 1102 18 -2.009283210946254 1102 19 -1.3808649768784775 1102 20 -0.9634002332287821 1102 21 -0.9161057130159058 1102 22 -0.661203542769087 1102 23 -0.6370838469838489 1102 24 -0.7238715747631872 1102 25 -0.47471054030944515 1103 1 -0.8504666223182866 1103 2 0.9957977014513801 1103 3 2.050352079811667 1103 4 2.6503833625321214 1103 5 2.326139948762247 1103 6 1.555575993275692 1103 7 1.6892748305471983 1103 8 3.0912736173868716 1103 9 4.144068312148942 1103 10 2.6039385973454223 1103 11 2.178815039957737 1103 12 3.3173134817435113 1103 13 3.40066964938649 1103 14 -2.8755324381558047 1103 15 1.7013749147398585 1103 16 2.358745310018193 1103 17 2.4018476776563276 1103 18 2.087738308799956 1103 19 1.7433450498494736 1103 20 1.7925151990395953 1103 21 1.5559204903663044 1103 22 1.574981292242029 1103 23 2.0333318100990567 1103 24 1.87323737285503 1103 25 1.91611390452357 1104 1 1.091381748609031 1104 2 0.4801210097220583 1104 3 1.0713921800622934 1104 4 1.427463563033374 1104 5 0.830444460040443 1104 6 -0.01580871850375587 1104 7 -0.2398166481495423 1104 8 1.3481219883141904 1104 9 -3.996338956021482 1104 10 -1.1069385956370326 1104 11 -0.7227513760823605 1104 12 0.8714864140743401 1104 13 0.9948989726947595 1104 14 0.8456016470572294 1104 15 -4.198600022603497 1104 16 -5.6406093265732835 1104 17 -2.149336532869988 1104 18 -1.46205492428275 1104 19 -1.0932965541605526 1104 20 -0.8973030966997513 1104 21 -1.282212341713894 1104 22 -1.0982831187855382 1104 23 -1.3916305959159807 1104 24 -7.713498216117401 1104 25 -4.550214416278436 1105 1 -2.6487633892719558 1105 2 -0.3213317513533447 1105 3 0.7443916962693571 1105 4 1.3225959149581783 1105 5 1.0910236292988564 1105 6 0.9808925048012398 1105 7 0.0787692942099838 1105 8 2.5895160031044284 1105 9 2.9475590327821664 1105 10 1.5824380026074891 1105 11 1.5924293551361046 1105 12 2.058476530875776 1105 13 2.2200727925855617 1105 14 2.718587340608959 1105 15 3.281290778419379 1105 16 2.7513375944302396 1105 17 1.4371870693139925 1105 18 1.6110897298053004 1105 19 0.573226356890661 1105 20 0.6692737227072563 1105 21 0.7968007206953942 1105 22 0.45444478224424856 1105 23 0.9941327602247334 1105 24 1.0312851137602692 1105 25 1.235958781592874 1106 1 2.698204818012748 1106 2 2.0453102291048246 1106 3 2.7162699904239744 1106 4 2.951025914660242 1106 5 2.1250373939882152 1106 6 1.5109515105145377 1106 7 1.51039700916089 1106 8 3.0765635467557466 1106 9 4.22540940314623 1106 10 2.574210685189666 1106 11 1.742707358731213 1106 12 2.582370368829263 1106 13 -3.9141556286179053 1106 14 0.7353335280794298 1106 15 2.7730882890848143 1106 16 2.5761229733848197 1106 17 2.081677292014806 1106 18 1.9891767472715038 1106 19 1.759305037795876 1106 20 1.677435068360996 1106 21 1.0565755370739822 1106 22 1.1967846091854142 1106 23 1.3570902531274491 1106 24 1.396979370811799 1106 25 1.478505036311983 1107 1 1.9635568061937647 1107 2 1.5518908588265568 1107 3 2.2578120550211622 1107 4 2.0812918900206334 1107 5 1.7433594226691262 1107 6 0.9166215922187533 1107 7 0.2880374391912177 1107 8 2.2098908972598004 1107 9 3.426658270509801 1107 10 1.8952251800410558 1107 11 0.8174145619611686 1107 12 2.371181802595 1107 13 2.660610437932384 1107 14 -3.815827864530231 1107 15 1.0242025910382075 1107 16 1.2653917076014913 1107 17 0.9161676273330883 1107 18 1.1938285900311705 1107 19 0.7979806516872365 1107 20 0.8850783257606416 1107 21 0.3286596556536594 1107 22 0.4414451752961597 1107 23 0.8653557247722148 1107 24 1.065968243714309 1107 25 1.1882982523625643 1108 1 1.15464584356452 1108 2 0.6918971860679655 1108 3 0.9961894105271796 1108 4 1.3946968539758622 1108 5 0.6067705869303666 1108 6 -0.012854076706386053 1108 7 -0.4519178209628798 1108 8 -4.518122182944818 1108 9 -5.860606300941647 1108 10 -2.611817781732835 1108 11 -0.9944696182753816 1108 12 0.24713711352325873 1108 13 1.5891931651491902 1108 14 1.943254607351136 1108 15 -3.9915461862321564 1108 16 -0.6093616227439789 1108 17 0.13836023129562136 1108 18 0.04859789470834741 1108 19 -0.16283732002179363 1108 20 -0.20316716339468144 1108 21 -0.43991758404821724 1108 22 -0.4972713906521484 1108 23 -0.34632467532816236 1108 24 -0.11961521484567683 1108 25 -0.015931528048959365 1109 1 0.6190221251101669 1109 2 0.5204159047004957 1109 3 0.8352253511106547 1109 4 -1.302317584089547 1109 5 -2.9049957959061423 1109 6 -1.1272933941656476 1109 7 -0.9308881926388075 1109 8 1.4042906719512267 1109 9 -4.135200179186505 1109 10 -1.017780622808175 1109 11 -0.42094537617509786 1109 12 -6.908891941747054 1109 13 -1.7425832918761124 1109 14 0.10722310576729299 1109 15 1.5324344281777869 1109 16 1.146725026837018 1109 17 0.6980984372232122 1109 18 0.5144689316081958 1109 19 -0.10493192301111165 1109 20 -0.2463595255030171 1109 21 -0.17745414632278766 1109 22 -0.25884972517492955 1109 23 -0.06106862624008613 1109 24 -0.46239189627690536 1109 25 -0.20913684406162503 1110 1 0.8979317971998952 1110 2 0.7366613401399391 1110 3 -2.7645359054576275 1110 4 -2.1940427537790654 1110 5 0.12064854167309491 1110 6 0.14460916293453252 1110 7 -0.039214121424567594 1110 8 -4.7049100816566565 1110 9 -7.791615923951511 1110 10 -2.825898751127802 1110 11 -0.5746950106790578 1110 12 1.9190665890014529 1110 13 2.223898000877462 1110 14 2.456320791858005 1110 15 3.192206585754132 1110 16 2.6667570485481917 1110 17 1.9972232338938625 1110 18 1.5870510038027703 1110 19 0.7873837179482912 1110 20 0.7273810626782833 1110 21 0.5995563657690965 1110 22 0.15071590939716017 1110 23 0.6151818433454359 1110 24 0.8604279257679224 1110 25 1.1662481687555761 1111 1 1.1224301095308107 1111 2 0.9517693613704097 1111 3 1.1302594309548617 1111 4 1.89972720656284 1111 5 -1.8037299731213317 1111 6 -0.30207430945289104 1111 7 -0.6582494389873972 1111 8 1.7153909640641771 1111 9 3.2800870652146656 1111 10 1.6652768260720592 1111 11 1.3823652372620998 1111 12 2.6753339589345444 1111 13 2.7856542933235 1111 14 3.1578560171596983 1111 15 3.4423662684893763 1111 16 2.47145725663254 1111 17 1.7745346592290545 1111 18 1.2501067115497726 1111 19 0.8921469474854824 1111 20 0.9099209029293114 1111 21 0.5620784729566672 1111 22 0.6379370323713189 1111 23 0.8600608771678964 1111 24 0.8199839557192021 1111 25 0.720756047856586 1112 1 2.0769044112616477 1112 2 1.3179548567581136 1112 3 2.1651085205874665 1112 4 2.36619650929768 1112 5 1.8134765007543692 1112 6 0.9567378134055424 1112 7 0.6547892343439479 1112 8 -3.680525659405209 1112 9 1.5882217684188191 1112 10 0.916305377315922 1112 11 1.043357385535682 1112 12 2.2447385106982374 1112 13 2.4937277304899013 1112 14 2.6230252487106327 1112 15 3.4232426171116934 1112 16 2.338172600648182 1112 17 1.7016698466010176 1112 18 1.2966053868060605 1112 19 1.2521276687147491 1112 20 0.9307044535355935 1112 21 0.8221261764329526 1112 22 0.5615152230298612 1112 23 1.2616695259390838 1112 24 1.0996810063995035 1112 25 1.1052028775834124 1113 1 0.945871247465341 1113 2 1.2292538855798227 1113 3 -6.134174465810027 1113 4 -2.7368387867274926 1113 5 0.07613379254200225 1113 6 0.24442625758274872 1113 7 1.0681101557597934 1113 8 2.7088061222297632 1113 9 -5.57070784768295 1113 10 -0.5473648796877697 1113 11 1.0119855754668166 1113 12 -4.0731673600990845 1113 13 1.275922054416533 1113 14 2.508531570118155 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-2.071192603902656 2204 10 0.681589145734744 2204 11 1.9455098733715488 2204 12 3.812010660470147 2204 13 4.110665683048627 2204 14 4.196422966410658 2204 15 4.72695262047208 2204 16 4.151365334850919 2204 17 3.2877145811536046 2204 18 3.1620735160842237 2204 19 2.562107552436217 2204 20 2.456017130473686 2204 21 2.0829864350445177 2204 22 2.011751830974184 2204 23 2.4179631131272714 2204 24 2.5031771879891984 2204 25 2.5012228925900644 2205 1 -3.404142330711082 2205 2 -3.8079458997612825 2205 3 -0.31819233362986665 2205 4 -2.4353328756554156 2205 5 -2.975148417337138 2205 6 -1.2235127519584985 2205 7 -0.8260516407301424 2205 8 -5.172084405858211 2205 9 0.8796923403497559 2205 10 0.46158022265515797 2205 11 0.4777005327264874 2205 12 2.2700591916272863 2205 13 -4.678704156942363 2205 14 -0.5324895440934547 2205 15 2.1419611537083867 2205 16 1.7362502444505914 2205 17 1.5949734630830683 2205 18 0.5295692494660713 2205 19 0.6666306008374256 2205 20 0.5432203081296016 2205 21 0.2339727385505057 2205 22 0.5816494321385122 2205 23 0.7305156336806746 2205 24 0.29814214540964556 2205 25 0.784100975600694 2206 1 -6.6923757032731 2206 2 -5.6363561053958335 2206 3 -2.2740055375326627 2206 4 -0.7883256673240737 2206 5 -0.6061041931459208 2206 6 -1.255400172867823 2206 7 -1.8063772243473188 2206 8 -2.899962056749928 2206 9 -5.613017059046037 2206 10 -2.432396171615814 2206 11 -1.6145549140535032 2206 12 -7.7725927157891554 2206 13 -6.412587705164938 2206 14 -7.384342387787164 2206 15 -2.2288937180070576 2206 16 -6.254643440985529 2206 17 -2.8728119690332115 2206 18 -1.9615490479414106 2206 19 -2.1120093555815806 2206 20 -2.273817197485698 2206 21 -2.013747003611357 2206 22 -2.1637998231678566 2206 23 -1.6212597659283894 2206 24 -1.4997232447082958 2206 25 -1.6191532682627674 2207 1 -6.765705086124861 2207 2 -5.064678243312489 2207 3 -0.8817637489759882 2207 4 0.24728615873832283 2207 5 0.8013875039246621 2207 6 0.2254891339705658 2207 7 0.1925643042314107 2207 8 1.7725570956904733 2207 9 -1.706572382725497 2207 10 -0.3960982595025797 2207 11 0.004746289650597646 2207 12 -4.174227871639946 2207 13 -4.908126237389272 2207 14 -0.6314571764721855 2207 15 -4.778562674330857 2207 16 -0.6352685235854238 2207 17 0.22945898533729547 2207 18 0.4660216396761676 2207 19 0.0600928015817459 2207 20 0.1715110956438508 2207 21 0.002250776866869586 2207 22 0.0887196823349079 2207 23 0.02666072061062863 2207 24 -0.021686824894246115 2207 25 0.47337101789523217 2208 1 -4.216534527554668 2208 2 -4.966621526092641 2208 3 -1.6620442460545455 2208 4 -0.1877008945022176 2208 5 -3.293692639972946 2208 6 -1.372603023854765 2208 7 -0.8895082623772375 2208 8 -5.475139046183958 2208 9 -8.347042753941775 2208 10 -4.062364578588788 2208 11 -1.6807087108438203 2208 12 -0.06223162046843811 2208 13 1.0096988120306811 2208 14 1.0031278665422114 2208 15 1.3882085639989015 2208 16 -4.3694333547787565 2208 17 -7.216158061856465 2208 18 -3.027012749962225 2208 19 -2.429809251085622 2208 20 -1.3423470561767026 2208 21 -1.4442054688616608 2208 22 -1.0799736619193823 2208 23 -0.8031167386392362 2208 24 -0.74952222647131 2208 25 -0.3511953597336053 2209 1 0.5590596512307542 2209 2 0.6026624757466692 2209 3 -2.6323905914732855 2209 4 -0.11504847548989972 2209 5 0.3681357387991362 2209 6 0.013763245113150191 2209 7 0.011919574429377633 2209 8 1.4447538770247306 2209 9 -4.113441684753425 2209 10 -1.070306706926491 2209 11 -0.36876528726481084 2209 12 -4.869920755535077 2209 13 -0.1419843873908455 2209 14 1.1182634983145832 2209 15 2.051688901955089 2209 16 -3.466275458194474 2209 17 -0.31277420007170065 2209 18 0.07376904575470178 2209 19 -0.06939607261962166 2209 20 0.012367850330743835 2209 21 0.026570429821685337 2209 22 -0.041544220979226054 2209 23 0.4905844923665228 2209 24 0.07690841844653562 2209 25 0.08516011555677139 2210 1 -0.4368889977980146 2210 2 -3.0355171120078595 2210 3 -0.915446021143083 2210 4 -3.14637775096093 2210 5 -3.603775024693647 2210 6 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-6.567142129335377 2211 18 -2.4009583919491035 2211 19 -1.5985065331849633 2211 20 -1.1469393710782967 2211 21 -1.0697912233133948 2211 22 -0.7819417968772093 2211 23 -0.5196115840000637 2211 24 -0.6562701177552839 2211 25 -0.26872113292948185 2212 1 1.7088009976662555 2212 2 0.9231833384410528 2212 3 1.903779456665017 2212 4 2.047728946117253 2212 5 -1.6557701418849327 2212 6 0.0036602810980497225 2212 7 0.6180087564835368 2212 8 -1.5840961739138102 2212 9 2.4608371618432727 2212 10 1.702122140314998 2212 11 1.3755071206267004 2212 12 2.815934450052133 2212 13 2.993350241052571 2212 14 3.415842904406208 2212 15 3.8130501308174374 2212 16 2.6759006399317613 2212 17 2.0665716956452265 2212 18 1.9067645543203007 2212 19 1.3006143914277182 2212 20 1.368234579265863 2212 21 1.0094813785681183 2212 22 1.1483554402288192 2212 23 1.1767335387196738 2212 24 1.153861538409103 2212 25 1.5640567757202533 2213 1 2.7719681093483572 2213 2 2.3100818583062335 2213 3 2.8512710838226525 2213 4 3.251162916579762 2213 5 2.032961076629718 2213 6 0.28154992667515444 2213 7 1.4077605165595521 2213 8 -5.883225548788605 2213 9 1.2437429888023352 2213 10 1.44046466394596 2213 11 1.4328026619764564 2213 12 3.147633186358778 2213 13 -1.4054215226161766 2213 14 1.824260483440728 2213 15 -3.6693475414963324 2213 16 0.5773238428703991 2213 17 1.8259771337056314 2213 18 1.9415970833654335 2213 19 1.86176490445731 2213 20 2.125422934439282 2213 21 1.8428609533785192 2213 22 2.0184372672656323 2213 23 2.401346732682761 2213 24 2.2072784387127875 2213 25 2.4702699686801353 2214 1 2.0298131211580497 2214 2 1.2705225036529626 2214 3 0.7564248598008554 2214 4 1.617500911306017 2214 5 1.6779047775007605 2214 6 0.18160491361575737 2214 7 0.21287242748071122 2214 8 1.914947385139584 2214 9 2.441186720915958 2214 10 1.1858345136066646 2214 11 0.8180049264386469 2214 12 2.561066246007112 2214 13 1.0038718951970935 2214 14 2.021904168065632 2214 15 3.1126604815981835 2214 16 1.6100629423342774 2214 17 1.3036886772140477 2214 18 0.49519997767803403 2214 19 0.2806810368758619 2214 20 0.08957832253460651 2214 21 -0.031362668511281555 2214 22 -0.34778825417302883 2214 23 -6.092620325141006 2214 24 -2.37159345421264 2214 25 -0.9330248737947748 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 109757.0 3 5.0 4 869.0 5 4989.0 6 6639.0 7 6141.0 8 5552.0 9 4772.0 10 4347.0 11 3813.0 12 3729.0 13 3513.0 14 3370.0 15 3309.0 16 3453.0 17 3768.0 18 4528.0 19 5959.0 20 8700.0 21 12047.0 22 15871.0 23 19948.0 24 23623.0 25 27852.0 26 31679.0 27 35073.0 28 37870.0 29 43694.0 30 46456.0 31 51465.0 32 62892.0 33 75778.0 34 89529.0 35 99927.0 36 107199.0 37 100869.0 38 87803.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.22032194902185 16.51678189599903 13.849786002668468 24.41311015231065 2 16.079913906343688 20.23599505065454 38.88199554281634 24.802095500185427 3 16.964360761396733 26.191534586846004 30.362891311110474 26.481213340646793 4 10.36925536766861 15.12615170112738 37.384483054624404 37.120109876579605 5 11.996145426940762 38.47961010577545 34.44062761600549 15.0836168512783 6 32.785145888594165 34.185693729304276 18.967510965482568 14.061649416618991 7 30.509457464596814 31.27954238717658 20.693041757430183 17.51795839079643 8 26.496814071998248 32.08386822193926 23.330562458664414 18.08875524739808 9 27.3986009984942 14.003105231995159 22.403009179592853 36.19528458991779 10 15.793661869886211 29.36423660803678 31.798138210036054 23.043963312040958 11 36.362005180071634 22.00464264786955 22.216404947072427 19.41694722498639 12 23.515404632745444 24.860828665394145 30.58328002260722 21.0404866792532 13 28.792656879537876 20.217939293912053 26.982881770836947 24.006522055713127 14 22.279016228042043 19.608436293536695 28.575812052194628 29.53673542622664 15 23.47324489362532 27.164049735417635 25.603945549338867 23.758759821618177 16 24.527333686987937 26.945100323489946 24.952412652513548 23.575153337008565 17 23.574708718256314 27.00750805672908 25.916577532690642 23.50120569232396 18 23.28227104531637 26.180452444289582 27.185055937952086 23.35222057244197 19 24.959004316247466 25.72951515886911 26.157759711556793 23.153720813326633 20 25.359715037363195 25.3694265196302 25.890291465861537 23.38056697714507 21 26.13456114469371 24.95609800070382 25.295612573740623 23.61372828086185 22 25.494552168297695 25.403971673008748 24.973432610711903 24.128043547981658 23 24.52952200649437 25.32103459215994 25.80421180741565 24.345231593930034 24 24.55144997512149 25.431559821984283 25.639429753312655 24.377560449581573 25 24.162859842410477 25.43822542756343 26.27627575565823 24.122638974367867 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2637.0 1 2637.0 2 1677.0 3 717.0 4 717.0 5 717.0 6 979.0 7 1241.0 8 1241.0 9 1241.0 10 1429.0 11 1617.0 12 1617.0 13 1617.0 14 2209.5 15 2802.0 16 2802.0 17 2802.0 18 4951.5 19 7101.0 20 7101.0 21 7101.0 22 12276.0 23 17451.0 24 17451.0 25 17451.0 26 27013.5 27 36576.0 28 36576.0 29 36576.0 30 48844.5 31 61113.0 32 61113.0 33 61113.0 34 76136.0 35 91159.0 36 91159.0 37 91159.0 38 105134.0 39 119109.0 40 119109.0 41 119109.0 42 131137.0 43 143165.0 44 143165.0 45 143165.0 46 151170.0 47 159175.0 48 159175.0 49 159175.0 50 156235.0 51 153295.0 52 153295.0 53 153295.0 54 141608.5 55 129922.0 56 129922.0 57 129922.0 58 113768.5 59 97615.0 60 97615.0 61 97615.0 62 80440.0 63 63265.0 64 63265.0 65 63265.0 66 49117.5 67 34970.0 68 34970.0 69 34970.0 70 26452.5 71 17935.0 72 17935.0 73 17935.0 74 13051.0 75 8167.0 76 8167.0 77 8167.0 78 6283.5 79 4400.0 80 4400.0 81 4400.0 82 3181.5 83 1963.0 84 1963.0 85 1963.0 86 1460.0 87 957.0 88 957.0 89 957.0 90 661.5 91 366.0 92 366.0 93 366.0 94 213.5 95 61.0 96 61.0 97 61.0 98 35.0 99 9.0 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22199400408717934 2 0.23305912578622878 3 0.23781367026628908 4 0.3020432438787401 5 0.24498871011801646 6 0.27386176205147356 7 0.2722192830492709 8 0.26979878767760385 9 0.2810368019032009 10 0.27377531578819975 11 0.2710954816267112 12 0.2743804396311165 13 0.27351597699837826 14 0.26850209372849654 15 0.26651382967319853 16 0.26971234141433004 17 0.2674647385692106 18 0.2678969698855797 19 0.26003035992766177 20 0.30377216914421656 21 0.2664273834099247 22 0.26953944888778236 23 0.2743804396311165 24 0.2750720097373071 25 0.27299729941873535 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1156788.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.61348788039611 #Duplication Level Percentage of deduplicated Percentage of total 1 90.72376078266005 69.5066374718628 2 5.430458799789183 8.32092778885278 3 1.6304480362092852 3.747429325852071 4 0.6887495759157567 2.1107002914819915 5 0.372698904423621 1.4276881498547995 6 0.2383624408088785 1.0957066782031586 7 0.15098788016331366 0.8097395688885111 8 0.11653143293357648 0.7142303619793402 9 0.09118113410689342 0.6287134241537325 >10 0.5007859414664305 7.051688640324658 >50 0.03889256069966501 2.058741103305635 >100 0.016688406817347214 2.173021155519957 >500 3.4057800449681076E-4 0.15716604652487182 >1k 1.1352600149893692E-4 0.19760999319563297 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNN 2272 0.19640591015812747 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGACCG 95 3.0559022E-10 14.997537 5 CGGCGGA 45 6.751111E-4 14.780476 18 CGACCTA 45 6.751111E-4 14.780476 18 GGACCGA 85 1.718763E-8 14.527025 6 TACGGTG 40 0.0052837664 14.24766 5 ACATGGT 610 0.0 13.08549 3 AACTATC 80 1.9940362E-6 13.064885 19 GGTATCA 830 0.0 12.81995 1 CGCGGCG 60 4.0843102E-4 12.66898 16 ACACCTA 130 1.9826984E-10 12.421037 6 TTGCGCG 400 0.0 12.114711 18 CCCTAGA 105 2.7154783E-7 11.762522 1 CAACGGA 65 8.002573E-4 11.6954565 4 GTCCTAT 90 7.4514337E-6 11.61172 1 ATTTGCG 420 0.0 11.537819 16 TTTGCGC 420 0.0 11.536819 17 AATTTGC 415 0.0 11.447872 15 TAGGTTG 75 2.0760967E-4 11.398128 5 TGCGCGC 445 0.0 11.316671 19 TTACACC 265 0.0 11.1162 4 >>END_MODULE