FastQCFastQC Report
Thu 26 May 2016
SRR1364271_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364271_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1156788
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT17290.14946558920044123No Hit
TATCAACGCAGAGTACTTTTTTTTT15060.13018807249037853No Hit
GGTCGGGAGTGGGTAATTTGCGCGC14830.12819980843508058No Hit
GTGGTATCAACGCAGAGTACATGGG13580.11739402552585262No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACTA350.002232015616.2168679
GGACCGA601.5266869E-615.7718536
GTCGGTT601.5335827E-615.76503612
GACCGCC551.1701608E-515.4850937
TCGGGTA400.004978021614.37620517
TGGACCG400.005416000714.1946695
TGGTCGG707.5866956E-613.51347210
TACTAGC500.001542729913.2495032
ACCGCCC500.001543735613.2483578
ACCGTGC500.001543735613.2483578
TACTCCG751.5381042E-512.6174835
AGGACCG1052.1025699E-812.6174825
TCTAGAC1001.5169098E-712.3015153
GGACCGC854.1159947E-612.2463816
CTGGTCG701.1344125E-412.1626519
GGTCGGT701.1353283E-412.16159911
GTATAAA951.0872645E-611.9549551
GGTATCA10700.011.6756341
CGAGTGG658.3138223E-411.64237710
CGGTTTG751.9114162E-411.49995614
Produced by FastQC (version 0.11.5)